• Title/Summary/Keyword: Floral stalk

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Multiple Shoot Formation from Flower Stalk Tissue Culture of Phalaenopsis Hybrids (팔레높시스 화경절편조직 배양에 의한 다신초 형성)

  • Choi, Jin Young;Koh, Jae Chul
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.3
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    • pp.165-171
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    • 2009
  • This experiment was conducted to extend utility of in-vitro culture material stalk and to mass produce uniform plants in Phalaenopsis. The optimal concentration of hyponex medium and plant hormone were examined. The most effective concentration of the hyponex in the flower stalk tissue culture of Phalaenopsis was $4mg{\cdot}L^{-1}$. The most effective concentration of TDZ and BA on the formation of multiple shoots in Phalaenopsis flower stalk culture was $0.3mg{\cdot}L^{-1}$ and $5mg{\cdot}L^{-1}$, respectively. TDZ was more effective for formation of the shoots and multiple shoots than BA at the basic medium with hyponex $4g{\cdot}L^{-1}$.

Somatic Embryogenesis from Various Parts of Muscari comosum var. plumosum

  • Xudong He;Ko Jeong-Ae;Choi Jeong-Ran;Kim Hyung-Moo;Kim Myung-Jun;Choi So-Ra;Kim Young-Gon;Kim Dong-Hee;Kim Hyun-Soon
    • Korean Journal of Plant Resources
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    • v.19 no.3
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    • pp.427-431
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    • 2006
  • In vitro high-frequency plant regeneration of Muscari comosum var. plumosum through somatic embryogenesis was obtained via two developmental pathways: direct embryos and multiple shoots regenerated from embryogenic callus. Flower bud with pedicel, receptacle, petal and ovary wall, floral stalk and leaf as explants were cultured in MS medium supplemented with various plant growth regulators. Embryos formed directly from pedicel, receptacle and floral stalk. Depending on explant sources, the optimal medium was MS medium supplemented with 0.2 mg/L IBA and 0.3 mg/L BA, 3.0 mg/L IBA and 3.0 mg/L BA, and MS-free medium for pedicel, receptacle, and floral stalk, respectively. Multiple shoots regenerated from embryogenic cal]i which was initiated from petal, ovary and leaf were observed in MS medium with different concentrations and combinations of hormone. The most suitable medium for each type of explant was 3.0 mg/L IBA and 3.0 mg/L BA(petal and ovary) and 5.0 mg/L IBA and 5.0 mg/L BA (leaf) Furthermore, the combination of 0.1 mg/L 2,4-D and 1.0 mg/L BA was also good for all sources of explants not only for direct embryo formation, but also, for embryogenic callus induction.

Structure, Ontogeny and Biology of Nectaries in Luffa acutanqqula (L.) Roxb. var. amara (Lam.) Cl.

  • Haran Iyer, K.R.P.;Subramanian, R.B.;Inamdar, J.A.
    • Journal of Plant Biology
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    • v.32 no.2
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    • pp.101-107
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    • 1989
  • Luffa acutanqula var. amara exhibits floral and extrafloral nectaries. The floral nectaries are restricted to the torus of the male flowers, while the extrafloral nectaries are observed on foliage leaves, probract, outer surface of calyx and pedicels. The floral nectaries develop from a group of epidermal and sub-epidermal initials which differentiate into secretory and subsecretory zones respectively during further divisions. The extrafloral nectary initiates from a single papillate nectary initial which gives rise to mature nectary comprising stalk layer, secretory and subsecretory tissues. Both the floral and extrafloral nectaries are vascularized. Interactions between insect-visitors and the plant with special reference to their functions are also discussed.

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Volatile Flavor Composition of White-flowered Lotus by Solid-phase Microextraction (Solid-Phase Microextraction에 의한 백련의 휘발성 향기 성분 분석)

  • Choi, Hyang-Sook
    • The Korean Journal of Food And Nutrition
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    • v.30 no.2
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    • pp.363-370
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    • 2017
  • This study investigated the chemical composition of headspace gas from white-flowered lotus (Nelumbo nucifera Gaertner). Volatile flavor compositions of headspace from white-flowered lotus (floral leaf, stamen, flower stalk, stem) were investigated through the solid-phase microextraction method using polydimethylsiloxane-divinylbenzene fiber. The headspace was directly transferred to a gas chromatography-mass spectrometry. Sixty-three volatile flavor constituents were detected in the headspace of lotus floral leaves, and undecanoic acid (7.81%) was the most abundant component. Fifty-three volatile flavor constituents were detected in the headspace of lotus stamina, and isobutylidene phthalide (7.94%) was the most abundant component. Forty-four volatile flavor constituents were detected in the headspace of lotus flower stalks, and 3-butyl dihydrophthalide (11.23%) was the most abundant component. Fifty-nine volatile flavor constituents were detected in the headspace of lotus stems, and ligustilide (16.15%) was the most abundant component. The content of phthalides was higher in the headspace of flower stalks and stems, while alcohols and acids were the predominant compounds in lotus floral leaves.

Effect of Treatment of Natural Cool Air from an Abandoned Mine on Flower Bud Formation and Flowering of Doritaenopsis 'Happy Valentine' (고온기 폐광 자연냉풍 처리가 호접란의 화아분화와 개화에 미치는 영향)

  • Chae, Soo Cheon
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.1
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    • pp.44-48
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    • 2008
  • This experiment was conducted to examine the effect of injecting cool air from an abandoned mine during the summer time for the growth of Doritaenopsis. The air temperature of abandoned mine in Boryeong was $12{\sim}14^{\circ}C$. The day and night temperatures were set at $22^{\circ}C$ and $20^{\circ}C$, respectively, from June to August in the experimental plastic house. This temperature range was within the suitable range for floral induction in Doritaenopsis. Average outside temperature was $28.4{\sim}32.8^{\circ}C$. The 3% of the crop developed flower stalk in 20 days after the treatment initiation, 65% in 45 days, and 100% in 90 days. The flower stalk length was short (48.7cm) in 30 days and long (62.4cm) in 60 days of treatment. The flower stalk length became longer as time passed. Flower spike and number of florets per stalk displayed the same tendency. Number of nodes was 6~7 and was not affected by the period. The first blooming appeared on 15th of September at 45 days and blooming tended to appear late as the period is lengthened. When the cool air from an abandoned mine was injected, the crop formed flower stalk three months earlier and bloomed four months earlier than the untreated control.

Regrowth of Buds and Flower Bud Formation in Kiwifruit as Affected by Early Defoliation (조기낙엽에 따른 참다래(골드러쉬) 무착과 유목 액아의 발아와 착화)

  • Kwack, Yong-Bum;Kim, Hong Lim;Chae, Won-Byoung;Lee, Jae Han;Lee, Eung Ho;Kim, Jin Gook;Lee, Yong Bok
    • Korean Journal of Environmental Agriculture
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    • v.32 no.3
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    • pp.201-206
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    • 2013
  • BACKGROUND: Kiwifruit, which was introduced to Korea in late 1970s, is a warm-temperate fruit tree, whose leaves are easily damaged by wind because of their large size. To produce high quality fruits, efficient windbreak is necessary to protect leaves until harvest. In Korea, typhoons from July onwards usually influence the production of kiwifruit. Damages from typhoons include low fruit quality in the current year and low flowering ratio the following year. This study was conducted to investigate the effect of early defoliation of kiwifruit vines from July to October on the regrowth of shoot axillary buds the current year and bud break and flowering the following year. METHODS AND RESULTS: Scions of kiwifruit cultivar 'Goldrush' were veneer grafted onto five-year-old Actinidia deliciosa rootstocks, planted in Wagner pots (13L) and grown in a rain shelter. Kiwifruit leaves in the proximity of leaf stalk were cut by lopping shears to simulate mechanical damage from typhoon since only leaf stalks were left when kiwifruit vines were damaged by typhoons. Kiwifruit vines were defoliated from July 15 to October 14 with one monthintervals and degrees of defoliation were 0, 25, 50, 75 and 100%. All experiments were conducted in the rain shelter and replicated at least five times. Defoliation in July 15 resulted in a high regrowth ratio of 20-40% regardless of degree of defoliation but that in August 16 showed only 5.8% of regrowth ratio in the no defoliation treatment; however, more than 25% of defoliation in August 16 showed 17-23% of regrowth ratio. In September 15, regrowth ratio decreased further to less than 10% in all treatments and no regrowth was observed in October 14. Percent bud break of all defoliation treatments were not significant in comparison to 64.7% in no defoliation except for 42.1% and 42.9% in 100% defoliation in July 15 and August 16, respectively. Floral shoot in the no defoliation treatment was 70.2% and defoliation of 50% or less resulted in the same or increased floral shoot ratio in July 15, August 16, and September 15; however, defoliation in October 14 showed no difference in all treatments. In flower number per floral shoot, 2-3 flowers appeared in no defoliation and only 1 flower was observed when the vines were defoliated more than 50% in July 15 and September 15. In October 14, contrary to the floral shoot ratio, flower number decreased with increased defoliation. CONCLUSION(S): Therefore, it is suggested that dormancy of 'Goldrush' axillary buds, was started in August and completed in October. The effect of defoliation on bud break of axillary buds the following year was insignificant, except for 100% defoliation in July 15 and August 16. From July 15 to September 15, floral bud ratio was significantly reduced when more than 50% of leaves were defoliated compared to no defoliation. Also, the number of flowers per flower-bearing shoot the following year decreased by less than 50% when compared to no defoliation, and this decrease was more prominent in September 15 than July 15 and August 16.

A New Cymbidium Cultivar 'Orange Bowl' with Orange Colored Flower and Medium Sized Plant (오렌지색계 중형 심비디움 'Orange Bowl' 육성)

  • Kim, Mi-Seon;Rhee, Hye-Kyung;Park, Sang-Gun;Jung, Hyang-Young;Choi, Sung-Yul;Lim, Jin-Hee
    • Horticultural Science & Technology
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    • v.29 no.6
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    • pp.651-654
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    • 2011
  • Cymbidium 'Orange Bowl' (Lucky Rainbow 'Randevous' ${\times}$ 'Eastern Star') was developed from a cross between hybrids at the National Institute of Horticultural & Herbal Science, Rural Development Administration in 2006. A cross was made between the pink colored flower C. Lucky Rainbow 'Randevous' as maternal line and pure yellow colored flower, C. 'Eastern Star' as paternal line in 1995. The seed germination, cultivation, selection, and characteristic trials were conducted from 1996 to 2006. The line was named as Wongyo F1-18 and phenotype was characterized in 2006 as a new 'Orange Bowl'. The 'Orange Bowl' has having light yellow basal color (RHS, YO21D) and orange line (RHS, OR30B) on both of sepal and petal with red lip (RHS, OR30B). 'Orange Bowl' has about 10.9 flowers per flower stalk and flower size of 7.4 cm. General appearance of the petals and sepals is slightly incurved shape. The plant size is intermediate having erect peduncle. Blooming is started from the late of January (mid winter) under optimal culture condition. Leaf attitude and twisting is half- erect and very weak respectively. This hybrid has attractive floral arrangement, long flower stalk (71.8 cm) and vigorous growth. We expect that 'Orange bowl' has a great potential for exporting to Chinese market.

A Dark Purple Cymbidium Cultivar 'Purple Star' with Medium Sized Plant for Pot Flower (진자주색계 중형 심비디움 'Purple Star' 육성)

  • Kim, Mi-Seon;Rhee, Hye-Kyung;Park, Sang-Gun;Shin, Hak-Ki;Jung, Hyang-Young;Lim, Jin-Hee
    • Korean Journal of Breeding Science
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    • v.43 no.6
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    • pp.559-563
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    • 2011
  • Cymbidium 'Purple Star' (Miake 'Pieta' ${\times}$ 'Allstar Mariane') was developed from a cross between hybrids at the National Institute of Horticultural & Herbal Science (NIHHS), Rural Development Administration (RDA) in 2008. A cross was made between purple colored flower C. Miake 'Pieta' as maternal line and dark pink colored flower, C. 'Allstar Mariane' as paternal line in 1995. The seed germination, cultivation, selection, and characteristic trials were conducted from 1996 to 2002. The line was named as Wongyo 'F1-21' and phenotype was characterized in 2007 as a new cultivar. The 'Purple Star' has dark purple basal color (RHS, GP186A) on both of sepal and petal with red lip (RHS, RP59A). 'Purple Star' has about 10.7 flowers per flower stalk and flower size of 7.6 cm. General appearance of petals and sepals is slightly incurved shape. The plant size is intermediate having erect peduncle. Blooming is started from the late of January (mid-winter) under optimal culture condition. Leaf attitude and twisting is erect and weak respectively. This hybrid has attractive floral arrangement, long flower stalk (78.4 cm) and vigorous growth. 'Purple Star' has a great potential for exporting to Chinese market.

Double Flower Freesia 'Sweet Lemon' with Strong Fragrance (향기가 강한 연노랑색 겹꽃 프리지아 '스윗레몬(Sweet Lemon)' 육성)

  • Choi, Youn Jung;Joung, Hyang Young;Goo, DaeHoe;Kang, Yun Im;Lee, Young Ran
    • FLOWER RESEARCH JOURNAL
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    • v.26 no.4
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    • pp.216-220
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    • 2018
  • Freesia (Freesia hybrida Hort.) 'Sweet Lemon' was developed for the cut flowers in National Institute of Horticultural Herbal Science, Rural Development Administration, Korea in 2015. This hybrid was crossed and selected from 'Teresa' and 'Yvonne' in 2007. Morphological characteristics of the selected freesia hybrid were investigated for 5 years from 2009 to 2014, and then it was named 'Sweet Lemon' in 2015. 'Sweet Lemon' has double and lemon petals (RHS color chart Y2B). The average flower diameter was 6.8 cm and the average yield is 6.7. The growth of the plant is vigorous and the average plant height is 99.0cm, which is 34.7cm higher than the standard cultivar 'Yvonne'. The average floret number per stalk of 'Sweet Lemon' was 10.7, and stalk length was 7.7 cm. The average days to first flowering of 'Sweet Lemon', 126 days, was approximately 20 days earlier than the control cultivar. It's average vase life and yield is 8.7 days and 5.6 cormlets per plant, respectively. Totally 58 of volatile compounds were identified using gas chromatography-mass spectrometry (GC-MS), the major components were linalool, alpha-terpineol, alpha-selinene, and limonene.

Occurrence of Sword bean Scab Caused by Cladosporium cucumerinum in Korea

  • Kwon, Jin-Hyeuk;Kang, Soo-Woong;Park, Chang-Seuk
    • Mycobiology
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    • v.28 no.1
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    • pp.54-56
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    • 2000
  • A black scab disease occurred on sword bean (Canavalia gladiata) in plastic film houses around Chinju area during the spring season of 1999. The disease started from flower bud, then moved to flower stalk, pod, petiole, cirrus, stem and leaves. The lesions started with small dark brown spots then were gradually expanded. Severely infected plants reached 37.4% of whole plant covered with scab. Numerous conidia were produced on the diseased flower disk, pod, floral axis, stem and leaves. Most of the conidia were appeared to be readily dispersed in the air, but the mycelia were not suggested causing of sooty mold by ectoparasitism. A fungus was isolated from the diseased stem, and inoculated to healthy plants to satisfy the Koch's postulates and proved the fungus was the causal agent of the disease. The isolated fungus grew on potato dextrose agar, forming greenish black to pale brown colonies. Conidia were ellipsoidal, fusiform or subspherical, mostly one-celled but occasionally septated. The conidia were $3.9{\sim}34.1{\times}2.7{\sim}5.1\;{\mu}m$ in size and formed in long branched chains on the erected conidiophores which were pale olivaceous brown and variable in length between $7.2{\sim}210.7\;{\mu}m$ in size. Ramoconidia were $7.6{\sim}29.2{\times}3.2{\sim}14.4\;{\mu}m$ in size. The fungus was identified as Cladosporium cucumerinum based on the above morphological characteristics. The optimum temperature for mycelial growth and conidial formation was about 15 to $25^{\circ}C$. Cladosporium scab of sword bean caused by the fungi has not been reported in Korea previously.

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