• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2003년도 생물공학의 동향(XII)
• /
• pp.40-44
• /
• 2003

Soil-borne infectious disease including Pythium aphanidermatum and Rhizoctonia solani causes severe damage to plants, such as cucumber. This soil-borne infectious disease was not controlled effectively by chemical pesticide. Since these diseases spread through the soil, chemical agents are usually ineffective. Instead, biological control, including antagonistic microbe can be used as a preferred control method. An efficient method was developed to select an antagonistic strain to be used as a biological control agent strain. In this new method, surface tension reduction potential of an isolate was included in the ‘decision factor’ in addition to the other factors, such as growth rate, and pathogen inhibition rate. Considering these 3 decision factors by a statistical method, an isolate from soil was selected and was identified as Bacillus sp. GB16. In the pot test, this strain showed the best performance among the isolated strains. The lowest disease incidence rate and fastest seed growth was observed when Bacillus sp. GB16 was used. Therefore this strain was considered as plant growth promoting rhizobacteria (PGPR). The action of surface tension reducing component was deduced as the enhancement of wetting, spreading, and residing of antagonistic strain in the rhizosphere. This result showed that new selection method was significantly effective in selecting the best antagonistic strain for biological control of soil-borne infectious plant pathogen. The antifungal substances against P. aphanidermatum and R. solani were partially purified from the culture filtrates of Bacillus sp. GB16. In this study, lipopeptide possessing antifungal activity was isolated from Bacillus sp. GB16 cultures by various purification procedures and was identified as a surfactin-like lipopeptide based on the Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), high performance liquid chromatography mass spectroscopy (HPLC-MS), and quadrupole time-of-flight (Q-TOF) ESI-MS/MS data. The lipopeptide, named GB16-BS, completely inhibited the growth of Pythium aphanidermatum, Rhizoctonia solani, Penicillium sp., and Botrytis cineria at concentrations of 10 and 50 mg/L, respectively. A novel method to prevent the foaming and to provide oxygen was developed. During the production of surface active agent, such as lipopeptide (surfactin), large amount of foam was produced by aeration. This resulted in the carryover of cells to the outside of the fermentor, which leads to the significant loss of cells. Instead of using cell-toxic antifoaming agents, low amount of hydrogen peroxide was added. Catalase produced by cells converted hydrogen peroxide into oxygen and water. Also addition of corn oil as an oxygen vector as well as antifoaming agent was attempted. In addition, Ca-stearate, a metal soap, was added to enhance the antifoam activity of com oil. These methods could prevent the foaming significantly and maintained high dissolved oxygen in spite of lower aeration and agitation. Using these methods, high cell density, could be achieved with increased lipopeptide productivity. In conclusion to produce an effective biological control agent for soil-borne infectious disease, following strategies were attempted i) effective screening of antagonist by including surface tension as an important decision factor ii) identification of antifungal compound produced from the isolated strain iii) novel oxygenation by $H_2O_2-catalase$ with vegetable oil for antifungal lipopeptide production.

• 한국항공우주학회지
• /
• 제48권5호
• /
• pp.323-334
• /
• 2020

경계층 천이는 극초음속 비행체의 열공력 성능에 직접적인 영향을 미치기 때문에, 성능 해석과 설계를 위해 천이지점의 정확한 예측 능력이 필수적으로 요구된다. 본 연구에서는 극초음속 경계층 천이 예측을 위한 γ-Re_θt 모델을 개선하고 검증하였다. 천이 시작 위치 결정에 영향을 주는 간헐도 수송방정식 생성항의 계수를 압축성 경계층 상사해를 기반으로 마하수와 벽면온도, 자유류 정체온도 등에 대한 함수 형태로 구성하고 적용하였다. 기본 모델의 Re_θc와 F_length 상관관계식에 마하수에 따른 천이 시작 운동량두께 레이놀즈수와 천이구간의 길이 변화를 반영할 수 있도록 마하수에 대한 관계식을 추가로 결정하여 적용하였다. 실용적인 사용을 고려하여 제안 모델을 상용 CFD 코드에 적용하였으며, 검증을 위해 모델을 사용하여 극초음속 평판과 원뿔 경계층 해석을 수행하였다. 실험 결과와의 비교를 통해 마하수와 단위 레이놀즈수 변화에 대한 개선된 예측성능을 확인하였다.

• 한국운동역학회지
• /
• 제13권3호
• /
• pp.253-264
• /
• 2003

This study aims at finding the structure of spike technique by analysing comparatively the spike action by excellent and by non-excellent players throughout the section from a flying jump to the time of landing for the correct analysis of spike action and tries to help athletes and coaches to execute a scientific training. For the objected person of this study, six of H College athletes three of excellent athletes and three of non-excellent athletes, presently registered as athlete with the Korea Volleyball federation) were chosen, and the factors of analysis were analysed upon performance time of action by section, human body centered displacement, change of articulation angle, speed change of articulation of the upper limbs, uniformity of the articulation of the upper limbs upon impact, etc. The conclusion of this study is as follow: 1. In the time required for taking action, it shows to take $1.067{\pm}0.057$ seconds for the group of excellent athletes and $1.034{\pm}0.033$ seconds for the group of non-excellent athletes. Although there was not big difference between two groups in the performance time of action, it showed that the group of excellent athletes takes longer compared to the group of non-excellent athletes. And it was found by the result of this study that the group of excellent athletes stays longer in the duration of flight. 2. In the displacements of horizontal movement and vertical movement, it was found that the group of excellent athletes have moved more than the group of non-excellent athletes in the horizontal movement of the center of human body 3. In the angles of wrist and knee, it was found that the excellent athletes have shown little than the non-excellent athletes in the entire sections, but that in the angle of elbow, the non-excellent athletes have shown bigger than the excellent athletes.. 4. In the speed of the articulation of the upper limbs upon impact, it was found that the group of excellent athletes have shown bigger than the group of non-excellent athletes, and that in the maximum value of the articulation of the upper limbs, the maximum value for the hand was indicated upon impact and that forearm and upper arm have shown the maximum value just before the impact. 5. In the uniformity of articulation of the upper limbs at the time of impact, the group of excellent athletes showed bigger than the group of non-excellent athletes in all the articulations.

• 한국항공우주학회지
• /
• 제43권4호
• /
• pp.296-303
• /
• 2015

무인기 운용 환경을 고려하여 다양한 조성의 $NaBH_4$ 용액을 사용해 수소발생시스템의 성능 평가를 수행하였다. 먼저, 자발가수분해와 30분의 수소발생실험을 수행하였다. 수소의 손실, 안정한 수소 발생, $NaBO_2$의 석출, 전환 효율과 무인기의 운용을 고려하여 $NaBH_4$ 용액의 조성을 1 wt% NaOH + 25 wt% $NaBH_4$+74wt% $H_2O$로 결정하였다. 200 W급 연료전지 시스템을 위해 장시간 수소발생실험도 수행되었다. 비록 $NaBO_2$의 석출로 인해서 수소 발생률이 감소하였지만, 200 W 연료전지를 위한 수소를 3시간동안 발생(전환 효율: 87.4%)시켰다. 600 Wh의 에너지를 갖는 200 W급 연료전지 시스템의 에너지 밀도는 263 Wh/kg이었다. 기존 배터리 무인기에 비해 약 1.5배 이상의 체공 시간을 달성할 수 있다.

• 한국항공우주학회지
• /
• 제43권6호
• /
• pp.548-556
• /
• 2015

F-22, F-16 및 F-15와 같은 고성능 전투기는 전투성능의 극대화를 위해 고기동성(highly maneuverable)을 보유하고 있다. 이로 인해 고기동 시에 조종사는 고중력가속도(high gravity acceleration)에 노출되고 피로도(fatigue)의 증가로 임무효율의 저하가 발생하며, 심할 경우에는 의식상실(Gravity-induced Loss Of Consciousness, GLOC)에 직면할 수 있다. 선진 항공업체에서는 조종사가 고중력가속도에 견딜 수 있는 내성을 향상시켜 의식상실에 진입하는 것을 방지하는 다양한 기술을 항공기에 적용하고 있다. 특히, 가속도방호복(Anti-G Suit)은 GLOC으로 인한 의식 상실을 방지할 수 있을 뿐만 아니라, 전투 기동 시에 조종사의 피로를 감소시킴으로써 임무성공률을 향상시킬 수 있다. 본 논문에서는 초음속 고등훈련기 모델을 기반으로 하여 고기동 시에 중력가속도의 증가에 따라 AGS에 최적의 공기압을 제공할 수 있는 제어알고리즘을 개발하고 검증하였다. 이러한 결과는 추후에 체계개발이 진행될 한국형전투기개발사업(Korean Fighter eXperimental, KF-X)에서 핵심기술을 개발하는데 기여하리라 기대한다.

• 농약과학회지
• /
• 제16권3호
• /
• pp.195-201
• /
• 2012

본 연구는 경기도에서 유통 중인 수입과실류에 대한 잔류농약 분석을 수행하였다. 총 22품목 124건의 시료를 구입하여 GC/NPD-ECD, TOF/MS, UPLC/PDA 및 HPLC/FLD, LC/MS/MS를 이용하여 218종 잔류농약을 동시 다성분 분석법으로 분석한 결과 18건의 과실에서 잔류농약이 0.003~0.3 mg/kg 범위로 검출되었으나 기준초과 시료는 없었다. 과실의 부위별 특성에 따른 잔류농약정도를 알아보기 위해 농약이 검출된 과실류 중 14건에 대하여 분리 실험을 실시하였으며, 그 결과 과피 14건에서 0.03~1.5 mg/kg, 과육의 경우 2건에서 검출한계 미만으로 나타났다. 실험결과 모두 잔류농약허용기준 이내에서 검출되어 정상적인 방법으로 섭취할 경우 위해도는 매우 낮은 수준이었다. 그러나 미량이라도 장기간 섭취시 인체에 유해할 수 있으며, 검출빈도가 높은 성분들이 검출되므로 수입과실에 대한 지속적이고 체계적인 모니터링이 필요하겠다.

• 한국통신학회논문지
• /
• 제34권11C호
• /
• pp.1085-1097
• /
• 2009

본 논문에서는 VDL 모드-2 규격을 따르는 송신기와 수신기의 구조 및 설계방법 그리고 개발한 모뎀의 성능시험 결과에 대해 기술한다. VDL 모드-2에서 송신기 필터는 올림 코사인 필터를 사용하고 수신기에서는 일반 저역 통과 필터(LPF)를 사용(비 정합필터)하기 때문에 ISI 경감 효과는 없으나 스펙트럼 특성은 더 좋다. 이는 정합필터를 적용했을 경우보다 1~2 dB 정도의 BER 성능은 저하되나 협 대역 통신에서 이웃 채널에 간섭을 최소화하는 것이 더 중요하기 때문이다. 송신기에서 변조신호 생성 시 발생되는 아날로그 방식의 단점(I/Q 이득 불균형, DC 오프셋 등)을 최소화하기 위해 디지털 방식으로 생성하였으며 수신기도 디지털 IF 샘플링 기법을 통하여 디지털 하향변환기를 적용하였다. 본 논문은 기 제안된 일부 구조 및 알고리듬을 포함하며 모뎀 구성에 필요한 전반적인 구조와 설계 방법 그리고 모의실험 결과가 추가되었다. 개발된 모뎀은 VDR 장비에 통합되어 각종 기능 실험과 환경시험을 거친 후 지상통신시험을 실시하였고 비행통신시험결과 시속 870 km/h로 310 km까지 메시지 송수신이 정상적으로 이루어짐을 확인하였다.

• 한국항공우주학회지
• /
• 제50권7호
• /
• pp.445-454
• /
• 2022

항공기가 빙점 이하의 습도가 높은 구름대를 지날 때 액적이 항공기와 충돌하면 날개, 동체 등 항공기 구성품에 결빙이 발생한다. 특히 항공기의 날개에 결빙이 증식되면 공력 성능의 저하와 비행 안정성의 감소 등의 치명적인 안전 문제를 초래할 수 있다. 본 연구에서는 항공기 날개에 적용되는 고양력 장치인 다중 익형의 결빙 증식량이 최소가 되도록 형상 최적설계를 수행하였다. 3차원 Reynolds-Averaged Navier-Stokes 지배 방정식을 이용하여 공력해석을 수행하였고, 다물리 전산해석을 통해 결빙의 형상 및 증식량을 예측하였다. 최적설계의 목적함수는 결빙 증식량 최소화로 설정하였고, 설계변수는 Slat과 Flap의 전개 각도와 위치를 정의하는 형상 변수 6개를 선정하였다. 설계 과정에서 목적함수의 평가는 크리깅 근사모델을 사용하여 대체하였고 유전자 알고리즘을 적용하여 최적 형상을 도출하였다. 최적화를 수행한 결과, Slat과 Flap에 최적의 전개 각도와 위치를 적용하였을 때 결빙 증식량이 약 8% 감소하였다.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2022년도 추계학술대회
• /
• pp.312-312
• /
• 2022

Peanut hull as by-product has been discarded during peanut processing. However, peanut hull contains plenty of polyphenols that shows various physiological activities. The objectives of this study were to investigate anti-inflammatory and enzyme inhibitory activities of polyphenols from 'Sinpalkwang' peanut (Arachis hypogaea L.) hull. Compounds were isolated from methanol extracts of peanut hull by preparative-high performance liquid chromatography after identifying and quantifying polyphenols using Ultra performance liquid chromatography (UPLC) and UPLC-Quadrupole time-of-flight-mass spectrometry profiling. The structures of compounds were elucidated by one-dimensional [¹H, ¹³C] nuclear magnetic resonance (NMR) and two-dimensional NMR (correlated spectroscopy, heteronuclear single quantum coherence and heteronuclear multiple bond correlation). Three compounds were identified as 5,7-dihydroxy-4H-chromen-4-one (peak 2), luteolin (peak 4) and eriodictyol (peak 5). Significant differences in inflammatory mediator such as nitric oxide (NO), interleukin-6 (IL-6) and interleukin-1β (IL-lβ) in lipopolysaccharide stimulated Raw 264.7 macrophages and in enzyme (xanthine oxidase [XO] and α-glucosidase [AG]) inhibitory activities were observed between three compounds (p < 0.05). Peak 5 treated Raw 264.7 macrophages showed lower content of NO (16.4 uM), IL-6 (7.0 ng/mL), and IL-1β (60.6 pg/mL) than peak 2 (NO: 28.3 uM, IL-6: 11.3 ng/mL, IL-1β: 66.9 pg/mL) and peak 4 (NO: 24.7 uM, IL-6: 9.3 ng/mL, IL-1β: 62.6 pg/mL). Peak 5 showed higher XO inhibitory activity (84.7%) and higher AG inhibitory activity (52.4%) than peak 2 (XO inhibitory activity: 45.4%, AG inhibitory activity: 21.6%) and peak 4 (XO inhibitory activity: 37.9%, AG inhibitory activity: 37.5%) at concentration of 0.5mg/mL. This study suggests that peanut hull could be a potential source of anti-inflammatory and physiological materials while creating new use of discarded peanut hull as by-products concomitantly.

• 한국식품저장유통학회지
• /
• 제30권2호
• /
• pp.235-246
• /
• 2023

Yellowball (Citrus hybrid cv. Yellowball ) is a new citrus hybrid between Haruka (C. tamurana × natsudaidai ) and Kiyomi (C. unshiu × sinensis) and is known to possess strong antioxidant activity. However, detailed information on the antioxidant components of its peel has not yet been reported. This study evaluated the antioxidant activity of the peel and identified the antioxidant components by fractionating a methanolic extract of Yellowball peels using liquid-liquid extraction with n-hexane, ethyl ether (ether), ethyl acetate (EA), butanol, and water. The phenolic contents and antioxidant activities of the n-hexane, ether, and EA fractions were higher than those of the other fractions, and these fractions were further separated by semi-preparative high-performance liquid chromatography (HPLC). Four antioxidant peaks, EA1, EA2, EA3, and He1, were isolated and analyzed using ultra-performance liquid chromatography-quadrupole-time- of-flight mass spectrometry (UPLC-Q-TOF MS). Sinapoyl glucoside and hesperidin were identified in EA2 and EA3, respectively, and a polymethoxylated flavone (PMF) complex (5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone, natsudaidain, tetrameth- oxyflavone, and tangeretin) was identified in He1. A compound in EA1 with m/z 223.0246 [M-H] could not be identified and was named unknown2. The antioxidant activity of unknown2 (IC₅₀=69.17 ㎍/mL) was similar to that of Trolox, which was noted as a major antioxidant in Yellowball peel. Further studies on the antioxidant capacity of Yellowball peel are required; however, these results provide a foundation for using Yellowball peel as an antioxidant.