• Journal of Life Science
• /
• v.18 no.2
• /
• pp.284-286
• /
• 2008

Octopus minor (O. minor) is widely distributed along the coastal regions of Korea, but most of them are caught in southern waters which are associated with one of the important fisheries stock. At present, O. minor from China has been introduced to the fishery markets in Korea. Here, we attempt to discriminate their origin for Korea or China using molecular techniques. Based on the O. minor mitochondrial DNA sequence, we developed a PCR-based origin discrimination system. The assay specificity was assessed by testing four individuals of O. minor from Sangdong, China, as well as 20 additional O. minor from Namhae, Muan, Yeosu and Jindo, Korea. Only four isolates of O. minor originated from China tested as positive in our distinction system. All PCR-positive products yielded identical sequences from Chinese O. minor, whereas Korean O. minor appeared to be PCR amplification. This result suggested that the primers used in this study are O. minor species specific, especially originated from China. The detection system appeared to be positive results in the use of 0.1 ng of Chinese O. minor DNA as template, however, the Korean O. minor even using $1{\mu}g$ of DNA showed no amplification. Consequently, the assay provides a simple, rapid and accurate method for the detection of Chinese O. minor.

• Journal of Radiation Protection and Research
• /
• v.41 no.1
• /
• pp.7-14
• /
• 2016

Background: The objective of this study was to investigate Korean people's awareness about impact of the Fukushima nuclear power plant accident in Japan and radioactive contamination caused by it. Materials and Methods: The respondents of the survey were 600 adults who resided in the Seoul metropolitan area. Results and discussion: The survey results show that the majority of respondents were concerned about impact of radiation leakage that might have an effect on our environment. They were worried about radioactive contamination of foodstuffs, particularly fishery products and preferred to acquire information through TV(49.8%) or the Internet(31.3%). Meanwhile, respondents mentioned that the information on the Fukushima nuclear accident and radioactive contamination had not been sufficient and they didn't know well about the follow-up measures of the government on the accident. Most respondents answered that information on radioactive contamination levels and safety of foods and environment was most needed. Conclusion: The results of this study could be useful to enhance awareness on radioactivity and improve risk communication on nuclear power plant accidents.

• Journal of fish pathology
• /
• v.27 no.2
• /
• pp.115-125
• /
• 2014

The effects of waterborne arsenic (As) exposure on antioxidant defense were studied in liver and gills of tilapia, Oreochromis niloticus under thermal stress. Tilapia were exposed to different As concentrations (0, 200 and $400{\mu}g/L$) at three water-temperatures (WT; 20, 25 and $30^{\circ}C$) for 10 days. In antioxidant response, glutathione (GSH) levels, glutathione reductase (GR), glutathione peroxidase (GPx) and glutathione S-treansferase (GST) activities were significantly decreased depend on WT in the gills after As exposure. Also, the range of fluctuation in these enzymes activities was most significantly increased at $30^{\circ}C$ in the liver of tilapia exposed to As. The present findings suggest that a simultaneous stress by temperature change and As exposure could accelerate the alteration in antioxidant enzymes activities of tilapia.

• Journal of Aquaculture
• /
• v.1 no.2
• /
• pp.121-133
• /
• 1988

Strongylocentrotus pulcherrimus is one of the very important fishery stock in this country since its high demand for raw gonad. Although the demand has been continuously increasing, the natural stock of this species seems to be decreasing. Since fresh macroalgae are not always available, the substitution of terrestrial vegetables for algae as a principal food was examined for the intensive indoor culture of the sea urchin. The results from the studies on selective and non-selective feeding habits and nutritional efficiencies using 13 food stuffs (6 macroalgae, 5 vegetables and 2 animal products) indicate that algae can be substituted with vegetables in sea urchin culture. Although the growth of body weight was the highest when sea urchins were fed Undaria Pinnatifida, the gonad index of the sea urchins fed on spinach, radish leaf and lettuce was higher than that of this brown alga. Considering the different food efficiencies of body and gonad growth, spinach and radish leaf will be adequate during gonad growth season, while lettuce and Chinese cabbage will be suitable for test growth season. In particular, radish leaf, which is usually not eaten by humans, could be highly beneficial and very economical in the sea urchin culture.

• The Journal of Fisheries Business Administration
• /
• v.46 no.3
• /
• pp.143-161
• /
• 2015

This study presents a comparative analysis on mackerel distribution process and price formation process, and investigation of price and margin between traditional markets and Large-scale discount store distribution channel. Through this, the study investigated distribution efficiency of each channel, and examined whether a difference of distribution efficiency leads to a difference of performance through the investigation of a difference of function and role between members of a wholesale market and vendor of Large scale discount store. The following are the results of this study. As a consequence of investigating supply and sum by distribution channel of mackerel, it appeared that mackerels shipped from port market are distributed into 9 consumption sites(Wholesale market, Large scale discount store, Institutional Food Service, etc.). In the comparison of distribution efficiency between traditional retail store and Large scale discount store 52.0% margin is formed in traditional retail store distribution channel and 43.1% margin is formed in Large scale discount store, and a distribution cost rate consists of 19.4% cost in a traditional retail store for fishery products and 18.1% cost in a Large-scale discount store. To analyze a difference of performance, the study examine a difference of role and function between vendor and Wholesale market company, wholesaler and middleman. Wholesale market company and middleman of wholesale market for consumer have slightly high or similar score in collection function, sorting function, evaluation function and financial function which are traditional and original. However, it was confirmed that vendor has a better score in other functions, that is, newly-demanded functions(ex : market frontier function, product development function, Integral Distribution Function, etc.).

• Fisheries and Aquatic Sciences
• /
• v.24 no.10
• /
• pp.319-329
• /
• 2021

In the search for antibiotic alternatives from safe and effective medicinal plants against fish pathogenic bacteria, we found that a combined extract (CE) of 1:1 (w/w) ratio of Angelica gigas Nakai roots and aerial parts of Artemisia iwayomogi Kitamura showed antibacterial activity against the fish pathogenic bacteria. By antibacterial activity-guided fractionations and isolations, five compounds were isolated and identified as decursinol angelate (1), decursin (2), xanthotoxin (3), demethylsuberosin (4), and 2,4-dihydroxy-6-methoxyacetophenone (5) through spectroscopic analyses, such as nuclear magnetic resonance (NMR) and mass spectrometry (MS). Among the compounds, 1 and 2 showed the highest antibacterial activities against Streptococcus iniae and Vibrio anguillarum, showing minimum inhibitory concentrations (MICs) of 62.5-250 ㎍/mL. Compounds 3, 4, and 5 were also found to be active, with MICs of 31.25-1,000 ㎍/mL for those strains. Furthermore, active compounds, 1 and 2 in CE were simultaneously quantified using high-performance liquid chromatography-tandem MS (HPLC-MS/MS). The average contents of 1 and 2 in CE was 3.68% and 6.14%, respectively. The established method showed reliable linearity (r² > 0.99), good precision, accuracy, and specificity with intra- and inter-day variations of < 2 % and recoveries of 90.13%-108.57%. These results may be helpful for establishing the chemical profile of CE for its commercialization as an antibiotic alternative in aquaculture.

• Journal of the Society of Disaster Information
• /
• v.18 no.1
• /
• pp.194-201
• /
• 2022

Purpose: This study investigated the relationship between climate factors and the catches in Korean offshore fisheries in recent three decades (1981 to 2010). Method: This study focused on seven types of fish species preferred in Korean cuisine. In the study, 10-year moving averages were used so that long-term trends could be easily identified. Results: Both air temperature and sea surface temperature (SST) on the coast of Korea rose in the period (p < 0.05). The rise in SST was significantly correlated with the rise in air temperature (p < 0.05), but not with precipitation. In the 2010s compared to in the 1981, catches of anchovy and squid greatly increased (p < 0.05), while catches of Alaska / walleye pollock has been almost extinct over the past 30 years. As such, cold-water fish species decreased or disappeared, and their fishing ground was replaced by warm-water fish species. Conclusions: These findings indicate that fish species caught in offshore fisheries of Korea have changed due to climate change, especially warming. This suggests that the warming of the Korean Peninsula may have a significant impact on the supply of fishery products and food security to Koreans in the near future.

• Fisheries and Aquatic Sciences
• /
• v.25 no.12
• /
• pp.601-618
• /
• 2022

The genus Semisulcospira is an economically and ecologically valuable freshwater resource. Among the species, Semisulcospira coreana, Semisulcospira forticosta and Semisulcospira tegulata are endemic to the Korean peninsula and Semisulcospira gottschei is widespread in Asia. Therefore, maintenance and conservation of wild populations of these snails are important. We investigated the genetic diversity and population structure of Semisulcospira based on the mitochondrial cytochrome c oxidase subunit I (COI), NADH dehydrogenase subunit 4 (ND4), and combined mitochondrial DNA (COI + ND4) sequences. All four species and various genetic makers showed a high level of haplotype diversity and a low level of nucleotide diversity. In addition, Fu's Fs and Tajima's D neutrality tests were performed to assess the variation in size among populations. Neutrality tests of the four species yielded negative Fu's Fs and Tajima's D values, except for populations with one haplotype. The minimum spanning network indicated a common haplotype for populations of S. coreana, S. tegulata and S. gottschei, whereas S. forticosta had a rare haplotype. Also, genetic differences and gene flows between populations were assessed by analysis of molecular variance and using the pairwise fixation index. Our findings provided insight into the degree of preservation of the species' genetic diversity and could be utilized to enhance the management of endemic species.

• Korean Journal of Food Science and Technology
• /
• v.45 no.5
• /
• pp.531-536
• /
• 2013

We developed a simple, sensitive, and specific analytical method for prometryn using gas chromatography-mass spectrometry (GC-MS). Prometryn is a selective herbicide used for the control of annual grasses and broadleaf weeds in cotton and celery crops. On the basis of high specificity, sensitivity, and reproducibility, combined with simple analytical operation, we propose that our newly developed method is suitable for use as a Ministry of Food and Drug Safety (MFDS, Korea) official method in the routine analysis of individual pesticide residues. Further, the method is applicable in clams. The separation condition for GC-MS was optimized by using a DB-5MS capillary column ($30m{\times}0.25mm$, 0.25 ${\mu}m$) with helium as the carrier gas, at a flow rate of 0.9 mL/min. We achieved high linearity over the concentration range 0.02-0.5 mg/L (correlation coefficient, $r^2$ >0.998). Our method is specific and sensitive, and has a quantitation limit of 0.04 mg/kg. The average recovery in clams ranged from 84.0% to 98.0%. The reproducibility of measurements expressed as the coefficient of variation (CV%) ranged from 3.0% to 7.1%. Our analytical procedure showed high accuracy and acceptable sensitivity regarding the analytical requirements for prometryn in fishery products. Finally, we successfully applied our method to the determination of residue levels in fishery products, and showed that none of the analyzed samples contained detectable amounts of residues.

• Journal of fish pathology
• /
• v.34 no.1
• /
• pp.71-80
• /
• 2021

Cephalexin, a semi-synthetic cephalosporin antibiotic, has long been used in fish aquaculture in various countries under legal authorization. The drug is thus widely available for use in other aquatic species except fishes like the crustacean whiteleg shrimp. This study aims to develop a sensitive method for laboratory residue studies to adopt in withdrawal period determinations. Through repeated trials from the existing methods developed for other food animal tissues, it was possible to achieve a sensitive high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method. The results showed that at a concentration of 0.1 mg/kg, the recovery rate was 81.79%, and C.V. value was 8.2%, which meet the recovery rate and C.V. recommended by Codex guideline. After satisfactory validation of analytical procedures, applicability to the shrimp tissue was confirmed in experimentally cephalexin-treated whiteleg shrimp. As a result, most muscle samples were detected below the limit of quantification (0.05 mg/kg) after day 3, and most hepatopancreas samples were detected below the limit of quantification after day 14. In particular, the limit of quantification 0.05 ppm with the presently developed method suggests sufficient sensitive over the current legal maximum residue limit of 0.2 mg/kg set for fishes.