Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Thermal effects on antioxidant enzymes response in Tilapia, Oreochromis niloticus exposed Arsenic

Arsenic에 노출된 틸라피아, Oreochromis niloticus의 항산화 효소반응에 미치는 수온의 영향

  • Min, EunYoung (Institute of Fisheries science, PuKyong National University) ;
  • Jeong, Ji Won (National Fishery Products Quality Management Service) ;
  • Kang, Ju-Chan (Department of Aquatic life medicine, Pukyong National University)
  • 민은영 (부경대학교 수산과학연구소) ;
  • 정지원 (국립수산물품질관리원) ;
  • 강주찬 (부경대학교 수산생명의학과)
  • Received : 2014.07.10
  • Accepted : 2014.08.04
  • Published : 2014.08.30
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Abstract

The effects of waterborne arsenic (As) exposure on antioxidant defense were studied in liver and gills of tilapia, Oreochromis niloticus under thermal stress. Tilapia were exposed to different As concentrations (0, 200 and $400{\mu}g/L$) at three water-temperatures (WT; 20, 25 and $30^{\circ}C$) for 10 days. In antioxidant response, glutathione (GSH) levels, glutathione reductase (GR), glutathione peroxidase (GPx) and glutathione S-treansferase (GST) activities were significantly decreased depend on WT in the gills after As exposure. Also, the range of fluctuation in these enzymes activities was most significantly increased at $30^{\circ}C$ in the liver of tilapia exposed to As. The present findings suggest that a simultaneous stress by temperature change and As exposure could accelerate the alteration in antioxidant enzymes activities of tilapia.

본 연구에서는 수온변화에 따른 비소 (As) 노출의 영향을 틸라피아 Oreochromis niloticus의 간과 아가미에서 항산화 방어기작 (antioxidant defense system)을 통해 알아보고자 한다. 틸라피아를 수온이 각각, 20, 25 및 $30^{\circ}C$ 일때, 비소 농도 0, 200 및 $400{\mu}g/L$에서 10일간 노출시킨 후, glutathione (GSH) levels, glutathione reductase (GR), glutathione peroxidase (GPx) and glutathione S-treansferase (GST) 효소 활성을 측정하였다. 비소 노출 이후, 틸라피아의 간과 아가미에서 이들 항산화 효소는 수온 변화에 따라 유의하게 변화하였다. 특히, 다른 온도구간에 비하여 수온이 $30^{\circ}C$ 일 때, 비소에 노출된 틸라피아의 간에서 이들 효소의 변동폭은 가장 유의하게 증가하였다. 즉, 본 연구는 틸라피아의 간과 아가미에서 GSH 및 항산화 효소인 GR, GPx 및 GST에 미치는 비소의 영향은 수온 상승이 동반되었을 때, 어류의 산화 스트레스에 대한 방어 기작의 감소를 촉진시켰음을 보여준다.

Keywords

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