• Journal of Environmental Health Sciences
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• v.39 no.4
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• pp.376-382
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• 2013

Objectives: The objectives of this study were to identify the virucidal efficacy against the viral hemorrhagic septicemia virus (VHSV) of an aquatic disinfectant tablet composed of calcium hypochlorite. Methods: Virucidal efficacy was determined through the viability of VHSV contacted with the disinfectant in a viral stock cultured in a fathead minnow cell line. An aquatic disinfectant tablet and VHSV were reacted under distilled water (DW), hard water (HW) or organic matter suspension (OM) conditions. Results: Under DW and HW conditions, VHSV was inactivated with 24,000- and 2000-fold dilutions of the aquatic disinfectant tablet, respectively. With the investigation of the antiviral effect of the disinfectant under OM conditions, VHSV was inactivated with a 16,000-fold dilution of the aquatic disinfectant tablet. Conclusions: The results from this study showed that the aquatic disinfectant tablet was a highly effective disinfectant against VHSV. In the future, a controlled field trial is required to determine whether the use of an aquatic disinfectant tablet will be able to reduce VHSV in a cultured marine fish farm.

• Journal of Korean Society of Rural Planning
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• v.26 no.2
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• pp.117-126
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• 2020

In this study, demands of smart technology development were analyzed for rural village communities. Questionnaire items were derived by grasping the current status of information and communication technology. 49 villages in 8 regions were selected and surveys and statistical analysis were conducted. The main results of the study are as follows. First, 92% of community leaders use smartphones, search for information (38%), communicate with the Internet (36%) using smartphones, use KakaoTalk (31%), and Facebook (24%). Second, in the rural and urban exchange activities, promote support information service (51%) and promote method suggestion service (48.5%) showed that the demand for services in promote field was high. It is linked to the creation of economic opportunities. Third, in the income and production activities, demand for distribution services technology (39.3%) was high in the field of production and distribution, and cold chains that help maintain freshness until food, such as meat, fish, and vegetables are delivered to consumers when agricultural products are distributed. The constant temperature control system needs to be actively introduced. Fourth, autonomy activities showed the highest demand for air conditioning and control systems (34.2%) of community building, and the lowest demand for electronic voting (9.4%) and videoconferencing (9.4%) services. Lastly, in the general activity area of the community, the demand for technology of emergency services (37.1%) and health self-diagnosis service (35.4%), which are technologies in the welfare sector, ranked first and second respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.52 no.5
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• pp.474-481
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• 2019

This study evaluated the chronic effects of $NO_3-N$ on juvenile blackhead seabream Acanthopagrus schlegelii. The experiment used six identically configured recirculating aquaculture systems (435 L), with three tanks (70 L) each. The $NO_3-N$ concentrations studied were 0 (control), 62.5, 125, 250, 500, and 1,000 mg/L $NO_3-N/L$. Thirty juvenile blackhead seabream ($18.8{\pm}2.2g$) were stocked in each tank. Growth and hematological changes were evaluated after 120 days. At the end of the experiment, the growth, survival, and cortisol levels indicated that blackhead seabream were healthy in 500 mg $NO_3-N/L$. However, insulin-like growth factor I (IGF-1) and the IGF-1 receptor were significantly lower at 250, 500, and 1,000 mg $NO_3-N/L$ than in controls (62.5 and 125 mg $NO_3-N/L$). Juveniles were likely affected at a much lower $NO_3-N$ concentration than 250 mg/L $NO_3-N/L$ in terms of IGF-1 and the IGF-1 receptor. Therefore, for the sake of long-term fish welfare, the $NO_3-N$ should be maintained at lower than 250 mg/L for blackhead seabream in recirculating aquaculture systems.

• Parasites, Hosts and Diseases
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• v.60 no.4
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• pp.289-293
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• 2022

Blastocystis is a genus of unicellular heterokont parasites belonging to a group of organisms known as Stramenopiles, which includes algae, diatoms, and water molds. Blastocystis includes several species that habitat in the gastrointestinal tracts of organisms as diverse as humans, farm animals, birds, rodents, reptiles, amphibians, fish, and cockroaches. It is important to public health and distributed globally, but its prevalence in dogs in Korea has not been reported to date. Here, we collected 787 canine fecal samples and assessed Blastocystis infection by age, sex, region, season, and diarrhea symptoms. We determined Blastocystis subtypes using phylogenetic analyses based on 18S rRNA gene sequences. We identified, 10 Blastocystis positive samples (1.3%). A higher proportion of infected dogs was asymptomatic; however, infection rates did not significantly differ according to region, age, sex, and season. Phylogenetic analysis showed that the Blastocystis sp. identified belonged to 4 subtypes (STs), ST1, ST5, ST10, and ST14, thus revealed the genetic diversity of Blastocystis sp. in dogs Korean. This is first report on the presence of Blastocystis sp. in dogs Korean. This study revealed a lower infection rate than expected and differed from previous studies in STs. Further studies are warranted to observe the national infection status of Blastocystis in dogs and the genetic characteristics of this genus.

• Journal of Nutrition and Health
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• v.48 no.3
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• pp.277-288
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• 2015

Purpose: As the rate of senior citizens living alone increases in the current aging society, there is much concern regarding the health and nutritional intake of solitary senior citizens. Therefore, this study compared the nutritional intake of senior citizens according to their family type. Methods: In July and August of 2011, two senior citizen welfare centers in Seoul were visited to survey 267 elderly women. Excluding 54 subjects for which the data were incomplete, information from 213 subjects was analyzed. The subjects were divided into three family types, living alone (LA, n = 74), living with spouse (LS, n = 78), and living with children (LC, n = 61). Results: The mean age of the LA group was the highest, while the mean age of the LS group was the lowest (p < 0.001), and WHR of the LC group was the highest (p = 0.049). Income was the highest in the LS group (p < 0.001). Frequency of eating out was the lowest in the LA group (p = 0.031). By Duncan's multiple analysis, the amounts of energy intake, vegetable protein, fat, calcium, phosphorus, potassium, selenium, Vit D, Vit E, $Vit\;B_2$, niacin, $Vit\;B_6$, $Vit\;B_{12}$, and cholesterol were significantly higher in the LS group compared with the LA or LC group (p < 0.05). The intakes of calcium, Vit D, $Vit\;B_{12}$, and cholesterol were still significantly different among the three groups, even after adjustment for age and monthly income. The LA group ate less fruit and fish than the LS or LC group (p < 0.05). The LA group showed the lowest dietary diversity and the LS group showed the highest diversity (p = 0.014), however, the significance of dietary diversity score among the three groups disappeared after adjustment for age and monthly income. Conclusion: Elderly women living with spouse were receiving better nutrition than elderly women living alone or living with children. Therefore, solitary elderly women who do not live with their spouse or children should be offered greater opportunities to receive a balanced meal at a congregational kitchen or welfare center. To ensure their healthy diet, it is essential to provide continuous nutrition education with these groups in mind.

• (The) Research of the performance art and culture
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• no.36
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• pp.751-781
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• 2018

Task of circling the pagoda of Waljeongsa(Woljeongsa Tabdori) is the major intangible cultural heritage with representativeness and historical meaning as a Buddhism culture, one of the Buddhism folk plays, which was firstly played after the liberation. Woljeongsa Tabdori holds significant designation importance in terms of Buddhism folklore heritage with Korean unique tradition and identity of Gangwon-do province. Temples are demonstrating Tabdori nationwide but Woljeongsa Tabdori is the unique case that systematically inherits the culture based on the designation of being intangible cultural heritage. That is why it is needed to focus on the cultural and internal value of Woljeongsa Tabdori. Tabdori is the integrated symbol of Buddhism respect and worship to the Buddha and pagoda. It is hard to presume the originality of Woljeongsa Tabdori: given the history of Woljeonsa temple, it lies into Goguryeo traditional play and Bokhui(Pagoda circling folk play) in Silla era. It fits into the courtesy of Circumambulating Stupa considering Moon in Goguryo mural, background of Odaesan Hwaeom thought/tripitaka and essence of Octagonal 9-story stone pagoda. At the first stage of Tabdori, Buddhist musical instruments such as Buddhism temple bell, singing bowl, cloud-shaped gong and wooden-fish. However, later, Samhyeon Yukgak has been added and then, Boyeom and Bakpaljeongjinga were singing: it could be interpreted that it was a pure Buddhist ceremony but it has become to have traditional aspect and been spread to the public. The origin of Woljeongsa Tabdori is related to the explanation of Circumambulating Stupa that experiences the glory of the ending ceremony. When a temple has a rite, the Buddhists make an offering to the Buddha. At that time, Buddhist prayer, sermon and chant are followed. After the rite, the Buddhists are circling the pagoda with the monks while praying for Buddhist charity and making their own wishes. It prays not only going after death to Nirvana of the one but also national prosperity and the welfare of the people for peaceful reign. As the temple holds bigger rites, many Buddhists gather and the Tabdori was a success. The scene of circling the pagoda and making own wishes in line with the Buddhist sermon was solemn. The idea on changes and convergence of Woljeongsa Tabdori requires strategic inheritance to promote the transmission while maintaining the paragon and purpose of designating the cultural heritage and reviving its identity. Korean Tabdori was held in Buddha's birthday in April and the mid-autumn day. Tabdori is a memorial service type Buddhist ceremony that once the monk holds the Buddhist rosary, circles the pagoda and sings the great mind and charity of the Buddha, Buddhists follow the step, lighting the lantern, circling the pagoda and praying for the gentle and easy death. Transmission education of the successor, diversified approach of the expert's advice and discourse on the revival of the origin should be reinforced in phases.

• Journal of Food Hygiene and Safety
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• v.36 no.4
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• pp.298-309
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• 2021

In this study we sought to develop a simultaneous analysis method for cis-bixin and cis-norbixin, the main components, to detect annatto pigment in food. To establish the optimal test method, the HPLC analysis methods of the European Food Safety Authority (EFSA), Japan's Ministry of Health, Labor and Welfare (MHLW), and National Institute of Food and Drug Safety Evaluation (NIFDS) were compared and reviewed. In addition, a new pretreatment method applicable to various foods was developed after selecting conditions for simultaneous high-performance liquid chromatography (HPLC) analysis in consideration of linearity, limit of detection (LOD), limit of quantification (LOQ), and analysis time. The HPLC analysis method of NIFDS showed the best linearity (R² ≥ 0.999), exhibiting low detection and quantification limits for cis-norbixin and cis-bixin as 0.03, 0.05 ㎍/mL, and 0.097, 0.16 ㎍/mL, respectively. All previously reported pretreatment methods had limitations in various food applications. However, the new pretreatment method showed a high recovery rate for all three main food groups of fish meat and meat products, processed cheese and beverages. This method showed an excellent simultaneous recovery rate of 98% or more for cis-bixin and cis-norbixin. The HPLC analysis method with a new pretreatment method showed high linearity with a coefficient of determination (R²) of 1 for both substances, and the accuracy (recovery rate) and precision (%RSD) were 98% and between 0.4-7.9, respectively. From this result, the optimized analytical method was considered to be very suitable for the simultaneous analysis of cis-bixin and cis-norbixin, two main components of annatto pigment in food.