Browse > Article
http://dx.doi.org/10.5668/JEHS.2013.39.4.376

Antiviral Efficacy of an Aquatic Disinfectant Tablet Composed of Calcium Hypochlorite Against Viral Hemorrhagic Septicemia Virus  

Park, Eun-Kee (Department of Medical Humanities and Social Medicine, College of Medicine, Kosin University)
Yu, Eun-Ah (Tongyeong National Quarantine Station, Ministry of Health & Welfare)
Cha, Chun-Nam (Engineering Research Institute, Department of Industrial Systems Engineering, Gyeongsang National University)
Yoo, Chang-Yeul (Department of Computer Information, Gyeongnam Provincial Namhae College)
Choi, Hyunju (Elderly Life Redesign Institute, Department of Biomedical Laboratory Science, Inje University)
Kim, Suk (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University)
Lee, Hu-Jang (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University)
Publication Information
Journal of Environmental Health Sciences / v.39, no.4, 2013 , pp. 376-382 More about this Journal
Abstract
Objectives: The objectives of this study were to identify the virucidal efficacy against the viral hemorrhagic septicemia virus (VHSV) of an aquatic disinfectant tablet composed of calcium hypochlorite. Methods: Virucidal efficacy was determined through the viability of VHSV contacted with the disinfectant in a viral stock cultured in a fathead minnow cell line. An aquatic disinfectant tablet and VHSV were reacted under distilled water (DW), hard water (HW) or organic matter suspension (OM) conditions. Results: Under DW and HW conditions, VHSV was inactivated with 24,000- and 2000-fold dilutions of the aquatic disinfectant tablet, respectively. With the investigation of the antiviral effect of the disinfectant under OM conditions, VHSV was inactivated with a 16,000-fold dilution of the aquatic disinfectant tablet. Conclusions: The results from this study showed that the aquatic disinfectant tablet was a highly effective disinfectant against VHSV. In the future, a controlled field trial is required to determine whether the use of an aquatic disinfectant tablet will be able to reduce VHSV in a cultured marine fish farm.
Keywords
Aquatic disinfectant tablet; viral hemorrhagic septicemia virus; calcium hypochlorite; disinfectant efficacy;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Karber G. Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. Arch Exp Pathol Pharmakol. 1931; 162(4): 480-487.   DOI
2 Mortensen HF, Heuer OE, Lorenzen N, Otte L, Olesen NJ. Isolation of viral haemorrhagic septicaemia virus (VHSV) from wild marine fish species in the Baltic Sea, Kattegat, Skagerrak and the North Sea. Virus Res. 1999; 63(1-2): 95-106.   DOI   ScienceOn
3 Ahne W. Vergleichende Untersuchung uber die Stabilitat von vier fischpathogenen Viren (VHSV, PFR, SVCV, IPNV). Zentralbl Veterinarmed B. 1982; 29(6): 457-476.
4 Kurita J, Iida Y, Nakajima K, Inouye K. Virucidal effects of various disinfectants on viral hemorrhagic septicemia virus (VHSV) isolated from Japanese flounder. Fish Pathol. 2002; 37(4): 175-181.   DOI
5 Jorgensen PEV. Inactivation of IPN and Egtved virus. Riv It Piscic Ittiop. 1973; 4: 107-108.
6 Ahne W. Studies on the tenacity of fish viruses. Fortschr Veterinaermed. 1982; 35: 305-309.
7 Corbeil S, Williams LM, Bergfeld J, Crane MSJ. Abalone herpes virus stability in sea water and susceptibility to chemical disinfectants. Aquaculture, 2012; 326-329; 20-26.   DOI   ScienceOn
8 Cha CN, Lee YE, Kang IJ, Yoo CY, Park EK, Kim S, et al. Antiviral efficacy of an aquatic disinfectant tablet composed to calcium hypochlorite against red sea bream iridovirus. J Fd Hyg Safety, 2012; 27(3): 285-289.   DOI   ScienceOn
9 Animal and Plant Quarantine Agency (APQA). The efficacy test guideline for disinfectants: Appendix 3. Protocols for the efficacy test of disinfectants against viruses. APQA Regulation No. 2013-34. Seoul: APQA; 2008.
10 Cornwell ER, Labuda SL, Groocock GH, Getchell RG, Bowser PR. Experimental Infection of Koi Carp with viral hemorrhagic septicemia virus type IVb. J Aquat Anim Health. 2013; 25(1): 36-41.   DOI   ScienceOn
11 Kim R, Faisal M. Emergence and resurgence of the viral hemorrhagic septicemia virus (Novirhabdovirus, Rhabdoviridae, Mononegavirales). J Adv Res. 2011; 2(1): 9-23.   DOI   ScienceOn
12 Office International des Epizooties (OIE). Viral haemorrhagic septicaemia. In: Aquatic Animal Health Standards Commission editor. Manual of diagnostic tests for aquatic animals, 6th ed. Paris: OIE; 2006. p.279-298.
13 Kim WS, Kim JH, Nishizawa T, Oh MJ. Pathogenicity of viral hemorrhagic septicemia virus (VHSV) isolated from olive flounder Paralichthys olivaceus to masu salmon Oncorhynchus masou. J Fish Pathol. 2012; 25(2): 117-121.   DOI   ScienceOn
14 Kim SM, Lee JI, Hong MJ, Park HS, Park SI. Genetic relationship of the VHSV (viral hemorrhagic septicemia virus) isolated from cultured olive flounder, Paralichthys olivaceus in Korea. J Fish Pathol. 2003; 16(1): 1-12.
15 Kasai H, Yoshimizu M, Ezura Y. Disinfection of water for aquaculture. Fish Sci. 2002; 68(S1): 821-824.   DOI
16 Lee WL, Yun HM, Kim SR, Jung SJ, Oh MJ. Detection of viral haemorrhagic septicaemia virus from marine fish in the South Western coastal area and East China sea. J Fish Pathol. 2007; 20(3): 201-209.
17 Ministry of the Environment Public Information Centre, Ontario. Guide to Eating Ontario Sport Fish 2011-2012. 26th ed. Ontario: Queen's Printer; 2011. p.14-22.
18 Deardorff TL, Kent ML. Prevalence of larval Anisakis simplex in pen-reared and wild-caught salmon (Salmonidae) from Puget Sound, Washington. J Wildl Dis. 1989; 25(3): 416-419.   DOI   ScienceOn
19 Kurita J., Iida Y, Nakajima K, Inoue K. Virucidal effects of various disinfectants on viral hemorrhagic septicemia virus (VHSV) isolated from Japanese flounder. Fish Pathol. 2002; 37(4): 175-181.   DOI
20 Phelps NBD, Goodwin AE, Marecaux E, Goyal SM. Comparison of treatments to inactivate viral hemorrhagic septicemia virus (VHSV-IVb) in frozen baitfish. Dis Aquat Org. 2013; 102(3): 211-216   DOI   ScienceOn
21 Yoshmizu M, Yoshinaka T, Hatori S, Hisae Kasal H. Survivability of fish pathogenic viruses in environmental water, and inactivation of fish viruses. Bull Fish Res Agen. 2005; S2: 47-54.
22 Kim WS, Kim SR, Kim DW, Kim JO, Park MA, Kitamura SI, et al. An outbreak of VHSV (viral haemorrhagic septicaemia virus) infection in farmed olive flounder Paralichthys olivaceus in Korea. Aquaculture. 2009; 296(1-2): 165-168.   DOI   ScienceOn
23 Trdo N, Benmansour A, Calisher C, Dietzgen RG, Fang RX, Jackson AO, et al. Family Rhabdoviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U., Ball LA. editors. Virus taxonomy: 8th report of the international committee on taxonomy of viruses, London: Elsevier Academic Press; 2005. p.623-644.
24 Kim WS, Jung SJ, Kim JO, Kim DW, Kim JH, Oh MJ. Genetic positioning of Korean viral hemorrhagic septicemia virus (VHSV) from cultured and wild marine fishes. J Fish Pathol. 2011; 24(1): 1-9.   DOI   ScienceOn