• 한국수산과학회지
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• 제57권1호
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• pp.23-31
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• 2024

This study evaluated the utilization of a low fish meal (LFM) diet and black soldier fly (BSF) Hermetia illucens meal and oil as a fish meal (FM) substitute or functional additive for juvenile olive flounder Paralichthys olivaceus at the Jeju fish farm. Two experimental diets replaced FM using animal (tankage, poultry byproduct and tuna byproduct meal) and plant (wheat gluten and soy protein concentrate) protein sources, containing 45% (FM45) and 35% (FM35) of FM, respectively. One experimental diet replaced FM with animal, plant, and BSF meal, fish oil using insect meal and oil (FM35+). After the feeding trial ended, no differences in growth performance, feed utilization, survival and biological indices were observed among all experimental groups. Aspartate aminotransferase and cholesterol levels in the FM35 and FM35+ groups were significantly higher than that in the FM70 group. The linoleic acid level in the muscle was significantly higher in the fish fed with the FM70 diet than in those fed with the FM45, FM35, and FM35+ diets. Thus, the LFM diet is suitable for juvenile olive flounder farming during six months.

• 한국양식학회지
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• 제10권2호
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• pp.143-151
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• 1997

본 연구는 조피볼락 사료에 있어서 어분대체품을 결정하고, 상품어분대체품과 유인물질의 첨가 효과를 통하여 조피볼락용 어분대체품개발을 위한 기초자료도 사용하고자 수행되었다. 실험사료는 조단백질 52%로, 가용성 에너지 16.8 KJ/g으로 설계되었고, 주단백질원으로 북양어분, 어분대체품 및 상품어분대체품을 사용하였다. 사료의 성분조성은 다음과 같이 요약된다 : 사료1, 100%FM (control) ; 사료2, 60%FM : 40% CFMA ; 사료3, 60%FM : 40% CFMA+ATT ; 사료 4, 80%FM : 20% FMA ; 사료5, 80% FM: 20%FMA+ATT : 사료 6, ; 사료6, 60%FM : 40%FMA+ATT; 사료7, 40%FM : 60% FMA+ATT. 어분대체품은 혈분(BM), 오징어간분(SLP), 육골분(MBM), 수지박(LM), 가금부산물(PBP), 우모분(FM), 필수아미노산(Met, Lys, Ile) 등을 적절히 배합하여 제작 사용하였다. 성장률, 사료효율, 일간성장률 및 단백질효율에 있어서 사료 1은 사료 4, 사료 5 및 사료 6에 비해 유의적인 차이가 없었지만(P>0.05), 나머지 실험구들은 대조구에 비해 유의적으로 낮게 나타났다(P<0.05). 특히, 상품어분대체품(CFMA)인 사료 2와 사료 3은 실험사료로 제작된 동일한 수준의 사료 6과 비교하여 성장률, 사료효율, 단백질효율에서 유의적으로 낮은 경향을 보였다. 0.5% 유인물질을 첨가한 사료 3과 5는 사료 2와 4에 비해 유의적인 차이는 보이지 않았으며(P>0.05), 간중량지수, hemoglobin 및 비만도에 있어서는 유의적인 차이가 나타났다(P<0.05). 따라서, 조피볼락에 있어서 어분대체품(FMA)으로 어분단백질을 40%정도 대체 가능함을 알 수 있었다.

• 한국양식학회:학술대회논문집
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• 한국양식학회 2003년도 추계학술발표대회 논문요약집
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• pp.70-70
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• 2003

This study was conducted to determine the effects of the dietary microbial phytase (P) supplementation on growth performance and bioavailability of phosphorus in juvenile olive flounder, Paralichthys olivaceus and juvenile Korean rockfish, Sebastes schlegeli fed soybean meal-based diets. Nine experimental diets were formulated to be isonitrogenous and isocalolic to contain 50.0%crude protein (CP) and 16.7kJ, 48.6% crude protein (CP) and 16.0kJ available energy/g without the dietary phytase supplementation for olive flounder and Korean rockfish, respectively : 100% fish meal (FM); 70% FM ＋ 30% soybean meal (SM); 70% FM ＋ 30% SM ＋ Phytase(P) 1000U/kg diet; 70% FM ＋ 30% SM ＋ P 2000U/kg diet; 70% FM ＋ 30% SM with phytase-treated (Ptre) P 1000U/kg diet; 60% FM ＋ 40% SM; 60% FM ＋ 40% SM ＋ P 1000U/kg diet; 60% FM ＋ 40% SM ＋ P 2000U/kg diet; 60% PM ＋ 40% SM with Ptre P 1000U/kg diet. After two weeks of the conditioning period, triplicate groups of 25 fish initially averaging 6.15$\pm$0.04g, was randomly distributed into the aquarium for olive flounder, and 20 fish initially averaging 7.25$\pm$0.04g was randomly distributed into the aquarium for Korean rockfish. After 8 weeks feeding trials, there was no significant difference on weight gain fish fed between 100% FM and 70% FM＋30%SM with Ptre P 1000U in olive flounder and Korean rockfish. Apparent phosphorus digestibility fish fed phytase supplemental diets were significantly higher than those of fish fed phytase non-supplemental diets in olive flounder and Korean rockfish. Therefore, these results indicated that pre-treated soybean meal with phytase 1000U could replace fishmeal up to 30% for the maximum growth of juvenile olive flounder and Korean rockfish. Phytase supplementation could improve apparent digestibility of phosphorus in olive flounder and Korean rockfish fed soybean meal based diets.

• 한국수산과학회지
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• 제53권3호
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• pp.423-431
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• 2020

This study was conducted to investigate in vivo and in vitro digestibility in juvenile Atlantic Bluefin tuna Thunnus thynnus. In vivo digestibility was compared between four experimental diets to determine the optimum dietary inclusion level of an enzyme-treated sardine fish meal (EFM) and sardine fish meal (FM). The experimental diets were as follows; EFM75 (75% EFM), EFM60 (60% EFM and 15% FM), FM75 (75% FM) and SL (frozen sand lance) as a raw fish feed. Feces of Bluefin tuna (90.3 g) were collected both by siphoning from a 700 L cage and by dissection in 69 ton concrete rearing tanks. For the siphoning method, protein digestibility was higher in the tuna fed SL diet than that of other groups. The lowest protein digestibility was observed in FM75. For the dissection method, protein digestibility was higher in tuna fed EFM75 diet than that of other groups. The lowest protein digestibility was observed in the EFM60 group. In vitro digestibility was compared in six protein sources to find an alternative source of EFM for the tuna feed. The highest in vitro digestibility was observed in EFM (92%) followed by low temperature FM (72%), meat meal (65%), feather meal (60%), sardine fish meal (57%) and poultry by-product meal (55%).

• 한국양식학회지
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• 제12권4호
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• pp.293-301
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• 1999

This study was conducted to evaluate the possible utilization of 5 different animal protein sources in juvenile rainbow trout, Oncorhynchus mykiss. Meat and bone meal (MBM), feather meal (FM), squid liver powder (SLP), poultry by-product(PBP) and blood meal (BM) were chosen to be the candidate for the possible ingredients for the dietary fish meal replacer in rainbow trout feed. Six different diets were formulated of isonitrogenous and isocaloric basis of $48\textperthousand$ crude protein and 16.7 kJ/g diet: diet 1, $100\textperthousand$ white fish meal (WFM); diet w, $80\textperthousand$ WFM +20% MBM; diet 3, 80% WFM +20% FM; diet 4, 80% WFM+20% SLP; diet 5, 80% SFM+20% PBP; diet 6, 80% WFM +20% BM. As the dietary protein sources, each diet containing 34.7% of animal protein were supplied by WFM with and without MBM, FM, SLP, PBP or BM and approximately 64.2% of plant protein. After one week of conditioning period, fish averaging 2g were divided into six groups and fed one of the experimental diets for 8 weeks. After eight weeks of feeding trials, there were no significant differences in weight gain and feed conversion ratio among groups of fish fed diet 1, 3, 4, 5 and 6(P>0.05). However, weight gain of fish fed diet 2 were significantly lower than those of fish fed diet 1, 3, 4, 5 and 6(P<0.05). These results indicated that FM, SLP, PBP and BM can be used as a dietary fish meal replacer up to 20% in juvenile rainbow trout.

• Asian-Australasian Journal of Animal Sciences
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• 제13권3호
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• pp.329-333
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• 2000

Data of 15 multiparous Holstein cows kept at Ibaraki Prefectural Animal Experiment Station were collected from 10 weeks prepartum to 20 weeks postpartum. Cows were assigned randomly to a soybean meal (S8M) diet or a fish meal (FM) diet from 4 weeks before expected calving date to 20 weeks postpartum. Each diet was formulated to contain similar amounts of CP, ADF, and NDF. In the FM diet, 2.5 and 5% of fish meal were supplemented as total mixed rations in prepartum and postpartum periods, respectively. Compared to the SBM diet, undegraded intake protein (UIP) and Met were higher in the FM diet, but Lys was low. Body weight and dry matter intake were not affected by supplemental FM, and dry matter intake increased by 6 weeks postpartum and maintained constant after 7 weeks postpartum. Cows in the FM diet remained high milk production during the experimental period, but milk yield in the SBM diet decreased gradually after 6 weeks postpartum. Supplemental FM increased milk yield and protein yield from 10 to 20 weeks postpartum when FM intake was 1.19 kg/d, although milk protein was not improved. There were no significance differences in fat content and fat yield between FM and SBM diets. Supplemental FM had no effect on plasma glucose and urea-N at parturition and 7 weeks postpartum. Thus, the increased milk and protein yield may be due to the combination of carryover effect of supplemental UIP or Met in FM from 4 weeks prepartum to 10 weeks postpartum and direct effect of supplemental FM.

• Fisheries and Aquatic Sciences
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• 제24권3호
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• pp.109-120
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• 2021

Protein hydrolysates and krill meal (KM) are used as protein sources in aquafeeds. The study was conducted to examine the supplemental effects of shrimp protein hydrolysates (SH) or KM in a high-plant-protein diet for red seabream (Pagrus major). A fish meal (FM)-based diet (40%) was considered as the high-FM diet (HFM) and a diet containing 25% FM and soy protein concentrate, in the expense of FM protein from HFM diet, was considered as the low fish meal (LFM) diet. Two other experimental diets (SH and KM) were prepared by including SH and KM into LFM diet at 5% inclusion levels in exchange of 5% FM from the LFM diet. A feeding trial was conducted for fifteen weeks using triplicate group of fish (Initial mean body weight, 8.47 ± 0.05 g) for a diet. Growth performance and feed efficiency of fish were significantly enhanced by HFM, KM and SH supplemented diets over those of fish fed LFM diet. Interestingly, these parameters of fish fed SH diet showed better performance than KM and HFM groups. Liver IGF-I expression of fish fed SH diet was comparable to HFM group and higher than KM and LFM diets. Protein digestibility of SH diet was significantly higher than KM, HFM, and LFM diets. Dry matter digestibility of SH diet was comparable to HFM diet and significantly higher than KM and LFM diets. Nitro blue tetrazolium and superoxide dismutase activities of HFM, SH and KM groups were significantly elevated than the LFM group and SH diet increased catalase and glutathione peroxidase activities of fish compared to KM and LFM groups. Hemoglobin level and hematocrit of fish fed SH and KM diets were significantly higher than LFM group. A diet containing 20% FM with KM is comparable to a HFM diet which contains 40% FM for red seabream. SH can be used to replace FM from red seabream diet down to 20% and fish performance can be improved better than a diet containing 40% FM. Overall, it seems that SH is more effective ingredient in red seabream diet compared to KM.

• 한국수산과학회지
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• 제51권4호
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• pp.376-382
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• 2018

We investigated the effect of replacing tuna byproduct meal (TBM) and rice bran (RB) with fish meal (FM) and macroalgae (MA) in extruded pellets (EP) supplied as a diet to juvenile Abalone Haliotis duscus in aquaculture. In total, 80,000 juvenile abalone were distributed among eight indoor raceways and supplied with one of four experimental diets. The control diet consisted of FM, fermented soybean meal, corn gluten meal and shrimp meal as protein sources, with wheat flour and dextrin as carbohydrate sources; the control diet also contained MA. In the FM50 diet, TBM was replaced with 50% FM. In the MA 50 diet, RB was replaced with 50% MA. The final diet, FM50+MA50, included TMB and RB in place of 50% FM and 50% MA. Abalone were fed to satiation with little food leftover for 16 weeks. Weight gain and specific growth rate of abalone fed the control diet were greater than those of abalone fed the FM50 and MA50 diets, but not different from those of abalone fed FM50+MA50 diet. The proximate composition of abalone soft body did not vary according to experimental diets. Based on these results, it appears that the traditional commercial diet for juvenile abalone, comprising FM and MA, could be replaced with one containing 50% TBM and 50% RB without any retardation of growth.

• 한국수산과학회지
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• 제53권3호
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• pp.464-470
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• 2020

This study was conducted to replace fish meal (FM) with three plant proteins (soybean meal, soy protein concentrate, and wheat gluten) in diets for growing olive flounder Paralichthys olivaceus. The control diet was formulated to contain 65% sardine FM and four other replacement diets were formulated to replace FM with the plant proteins by 25, 30, 35 and 40% (designated FM25, FM30, FM35 and FM40, respectively). The replacement diets were added with three essential amino acids (lysine, methionine and threonine) to meet their requirements for the fish. Olive flounder (initial average weight, 96.8±0.2 g) were randomly distributed into 20 tanks (425 L each) at a density of 25 fish per tank. Four replicate groups of fish were fed one of the diets two times daily for 15 weeks. At the end of the feeding trial, no significant differences were found among all the fish groups in growth performance, feed utilization, nonspecific immune responses and hematological health parameters. Thus, this result indicates that the plant proteins with the three limiting amino acids could replace FM up to 40% in diets for growing olive flounder.

• 한국수산과학회지
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• 제53권4호
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• pp.577-582
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• 2020

This study was conducted to investigate the replacement of fish meal (FM) with a plant and animal protein mixture (wheat gluten, soy protein concentrate, tankage meal and poultry by-product meal) in the diets of juvenile olive flounder Paralichthys olivaceus. The basal diet was formulated to contain 65% FM (Con). Four other experimental diets were formulated with alternative proteins replacing 20%, 30%, 40% and 50% of FM (FM20, FM30, FM40 and FM50, respectively). Taurine and betaine were added to the FM replacement diets. Triplicate groups of fish (mean±SD, 5.41±0.01) were fed the diets to apparent satiation for 15 weeks. After the feeding trial, no significant differences were found between any dietary groups in growth performance, feed utilization, survival, hematological parameters or whole-body composition. This result indicates that a proper mixture of the four protein sources with taurine and betaine supplements can be used as FM replacement to reduce FM levels from 65% to 32.5% in juvenile olive flounder diets.