• Title/Summary/Keyword: Fish bacteria

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Effect of β-glucan on immune parameters in the Manila clam Ruditapes philippinarum (β-glucan이 바지락의 면역력에 미치는 영향)

  • Nam, Ki-Woong;Park, Kyung-il
    • The Korean Journal of Malacology
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    • v.31 no.2
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    • pp.123-127
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    • 2015
  • ${\beta}$-Glucan is a polysaccharide that is widely used as an adductive in fish feed to facilitate immune stimulation. This study aimed to investigate the effect of ${\beta}$-glucan on immune responses in the Manila clam Ruditapes philippinarum. For this purpose, three groups of R. philippinarum were exposed to 0%, 0.1%, or 1% ${\beta}$-glucan in sea water for 1 hr/day for 2 weeks using an immersion method. Thereafter, two immune parameters-phagocytic rate and antibacterial activity-were measured. R. philippinarum exposed to 1% ${\beta}$-glucan showed an approximate 30% significant increase in phagocytic rate. In addition, ${\beta}$-glucan significantly limited the growth of the pathogenic bacteria Vibrio tapetis, V. parahaemolyticus, and V. ordalii. Moreover, the mortality rates of ${\beta}$-glucan-treated clams decreased during a 17-day experiment. Our study suggests that treatment with ${\beta}$-glucan significantly increases the immune responses in R. philippinarum, and that immersion is a simple and effective method for immune stimulation in this species.

Effects of Dietary Supplement of Probiotics on Growth and Blood Assay of Rockfish Sebastes schlegeli (생균제 첨가사료가 조피볼락 Sebastes schlegeli의 성장 및 혈액성분에 미치는 영향)

  • Park, Sang-Hyun;Wang, Soon-Young;Han, Kyung-Nam
    • Journal of Aquaculture
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    • v.21 no.1
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    • pp.1-6
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    • 2008
  • We determined the effects of dietary supplements for lactic acid bacteria(LAB) such as Lactobacillus brevis(Lb) and Lactobacillus plantarum(Lp) in juvenile rockfish Sebastes schlegeli cultured in flow-through system for 10 weeks. The experimental diets contained $10^4cfu/g,\;10^6cfu/g\;and\;10^8cfu/g$ level each LAB(Lb-4, Lb-6, Lb-8 or Lp-4, Lp-6, Lp-8), respectively. The effects of LAB supplementation was determined by various factor such as weight gain(WG), specific growth rate(SGR), feeding efficiency(FE) and blood assay. For rearing experiment, Lp-8 treatment had significantly high growth rate than control diet treatment. However, all Lb treatment had no significance effect with control diet treatment. In case of the blood assay, hematocrit(Ht) and hemoglobin(Hb) of fish were not affected by LAB supplemental levels. On the other hand, total cholesterol in plasma of Lb-8, Lp-6 and Lp-8 treatments were significantly low than the control diet treatment. We verified the influence of LAB which was originated from species specificity and amount in diet. Consequently, the dietary supplementation as $10^8cfu/g$ level of L. plantarum could be of help for growth enhancement to the juvenile rockfish.

Taxonomical Characterization and Antimicrobial Activity of Red Pigment-Producing Marine Bacterium Strain JE-34 (적색색소를 생산하는 해양미생물 JE-34 균주의 분류학적 특성 및 항균활성)

  • Kim, Ju-Sang;Kim, Man-Chul;Harikrishnan, Ramasamy;Han, Yong-Jae;Heo, Moon-Soo
    • Korean Journal of Microbiology
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    • v.45 no.4
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    • pp.368-376
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    • 2009
  • A red pigment-producing bacterial strain was isolated from sediment sample of the East China Sea. The isolate was identified by analysis based on 16S rDNA sequence and morphological, physiological properties, biochemical characteristics and fatty acid composition. Phylogenetic analysis based on 16S rDNA sequence showed that isolate represent a phyletic lineage within the genus Zooshikella, and this strain was most closely related to Zooshikella ganghwensis KCTC $12044^T$ (AY130994) (99.79%). The strain was Gram-negative, aerobic and required NaCl at 0.5~8.0% for growth. The predominant cellular fatty acids were saturated and monounsaturated straight-chain fatty acids. Consequently, this strain was identified as a member of the genus Zooshikella and designated as Zooshikella sp. JE-34. The pigment showed characteristics similar to prodigiosin, a well-known red pigment previously detected in Serratia marcescens. The antimicrobial activity of Zooshikella sp. JE-34 bacterial pigment was tested against 18 microorganisms, which were fish and human pathogens. The Zooshikella sp. JE-34 red pigment showed high antimicrobial activity against Streptococcus iniae, S. parauberis, S. mutans, Staphylococcus aureus, and Propionibacterium acnes.

Effect of Setting on the Texture Intensity of Smoked Alaska Pollock Roe Sausage with Cellulose Casing and Its Quality Characteristics during Storage (셀룰로오스 케이싱에 충전한 명란훈연소시지의 텍스쳐에 대한 세팅의 영향 및 저장기간에 따른 품질특성)

  • Park, Jong-Hyuk;Kim, Young-Myung;Kim, Sang-Moo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.1
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    • pp.96-103
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    • 2006
  • Alaska pollock roe is mainly used as the production salted instead of salt-seasoned seafood (Myungranjeot). Alaska pollock roes with broken egg membrane are usually discarded as a waste product. In order to utilize the broken roes of Alaska pollock, imitated fish sausage was manufactured for commercial production. Hardness, cohesiveness, elasticity, brittleness, and gumminess of Alaka pollock roe sausage were evaluated based on mixture design and regression models. The higher amounts of carrageenan and tile lower amounts of starch caused the higher the texture intensity of Alaska pollock roe sausage. The pHs of control, vacuum and $N_2$ packages, increased up to 6.28, 6.23 and 6.24, respectively, during 4 months storage and then decreased. The values of volatile basic nitrogen (VBN), thiobarbituric acid (TBA), and total viable cell counts increased during storage periods, while the parameters were higher in control than in vacuum and Na packages. Coliform bacteria was not detected in all treatments during storage periods.

Developing a Gene-trapping Approach for Gene Identification Using Nuclear Transfer in Zebrafish (지브라물고기 복제방법에 의한 유전자 동정 및 유전자트랩법 개발)

  • Lee, K.Y.
    • Journal of Animal Science and Technology
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    • v.46 no.2
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    • pp.155-164
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    • 2004
  • This involves identifying and cloning trapped genes from cultured cells carrying the gene-trap constructs and generating cloned zebrafish using these cells for functional study. Gene-trapping studies in gene-trapped cells were carried out in initial and cloned zebrafish carrying gene-trap events were successfully produced based on the nuclear transplantation technique. Two kind of retroviral gene-trap constructs were adopted. The first one(SA/GFP-TP), constructed in my laboratory, carries a GFP reporter gene containing a splicing acceptor and an internal neo gene. The second one(Neo-TP), obtained from Dr. Hicks (Hicks et al., 1997), contains a promoter-less neo gene located in the LTR sequence of a retroviral vector. The infected cells were subjected to drug selection(neomycin treatment) because the two constructs carry the neomycin resistant gene. All those cells survived the neomycin treatment should carry the proviral insertions. For Neo-TP, Isolated DNA from the neomycin-resistant fibroblast cells infected by Neo-TP, was digested with EcoR1 restriction enzyme and transformed into bacteria after ligation. This procedure led to the isolation of seven clones carrying flanking cellular DNA with a typical retroviral integration signature sequence. These clones contained genomic DNA ranging from 1kb to 7kb and sequences of 300-600 bp were obtained from each of the rescued plasmids. Database searching showed that all of them share high homology to zebrafish sequences. For fish cloning using tagged cells, initially, nucleus donors directly selected from a mixture of cells(Neo-TP cells) were used. A total of 44 embryos(3.7%) out of 1179 transplants were reached blastula stage; 8 of these embryos(0.8%) hatched and 3(0.3%) of them survived to adulthood. One out of three lived cloned zebrafish has an amplified fragment and was labeled with 32P.

Sulfate Reduction in the Marine Environments: Its Controlling Factors and Relative Significance in Mineralization of Organic Matter (해양환경의 황산염 환원율 조절요인 및 유기물 분해에 있어 황산염 환원의 중요성)

  • 현정호;이홍금;권개경
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.8 no.2
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    • pp.210-224
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    • 2003
  • Sulfate reduction is a microbiological process which occurs ubiquitously in anaerobic marine environment. Sulfate reducing bacteria play a significant role in anaerobic decomposition of organic matter and regeneration of inorganic nutrients which supports the primary production in the water column (i.e., benthic-pelagic coupling) and, in special case, could be responsible for the harmful algal bloom in the coastal marine environment. Summary of the sulfate reduction rates reported in various marine sedimentary environments revealed that supply of organic substrates and presence of various electron acceptors (i.e., $O_2$, NO$_{3}$$^{[-10]}$ , Fe(III) and Mn(IV), etc.) for other aerobic and anaerobic respiration directly affect the sulfate reduction rate and relative significance of sulfate reduction in organic matter mineralization. Significance of temperature, macrophytes and bioturbation is discussed as factors controlling supply of organic substrates and distribution of electron acceptors. Finally, we suggest studies on the anaerobic microbiological processes associated with biogeochemical element cycles in the coastal environments of Korea where massive operation of organic enriched fish cage farm, frequent occurrence of toxic algal bloom and hypoxia and conservation of tidal flat are of major environmental issues.

A Laboratory-Scale Study of the Applicability of a Halophilic Sediment Bioelectrochemical System for in situ Reclamation of Water and Sediment in Brackish Aquaculture Ponds: Establishment, Bacterial Community and Performance Evaluation

  • Pham, Hai The;Tran, Hien Thi;Vu, Linh Thuy;Dang, Hien The;Nguyen, Thuy Thu Thi;Dang, Thu Ha Thi;Nguyen, Mai Thanh Thi;Nguyen, Huy Quang;Kim, Byung Hong
    • Journal of Microbiology and Biotechnology
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    • v.29 no.7
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    • pp.1104-1116
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    • 2019
  • In this study, we investigated the potential of using sediment bioelectrochemical systems (SBESs) for in situ treatment of the water and sediment in brackish aquaculture ponds polluted with uneaten feed. An SBES integrated into a laboratory-scale tank simulating a brackish aquaculture pond was established. This test tank and the control (not containing the SBES) were fed with shrimp feed in a scheme that mimics a situation where 50% of feed is uneaten. After the SBES was inoculated with microbial sources from actual shrimp pond sediments, electricity generation was well observed from the first experimental week, indicating successful enrichment of electrochemically active bacteria in the test tank sediment. The electricity generation became steady after 3 weeks of operation, with an average current density of $2.3mA/m^2$ anode surface and an average power density of $0.05mW/m^2$ anode surface. The SBES removed 20-30% more COD of the tank water, compared to the control. After 1 year, the SBES also reduced the amount of sediment in the tank by 40% and thus could remove approximately 40% more COD and approximately 52% more nitrogen from the sediment, compared to the control. Insignificant amounts of nitrite and nitrate were detected, suggesting complete removal of nitrogen by the system. PCR-DGGE-based analyses revealed the dominant presence of Methylophilus rhizosphaerae, Desulfatitalea tepidiphila and Thiothrix eikelboomii, which have not been found in bioelectrochemical systems before, in the bacterial community in the sediment of the SBES-containing tank. The results of this research demonstrate the potential application of SBESs in helping to reduce water pollution threats, fish and shrimp disease risks, and thus farmers' losses.

Fermented Organic Matter as Possible Food for Rearing Anguilla japonica Leptocephali (뱀장어(Anguilla japonica) 자어 먹이로 유기물 분해산물의 활용 가능성)

  • Kim, Hyo-Won;Kim, Jung-Hyun;Kim, Myung-Hee;Kim, Kwang-Hyun;Park, Jin-Chul;Park, Heum-Gi;Han, Chang-Hee;Kim, Dae-Jung
    • Journal of Life Science
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    • v.28 no.12
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    • pp.1424-1431
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    • 2018
  • We prepared flocculated detritus-like organic marine snow originating from various organisms by fermentation using microorganisms; this fermented organic material was fed to the leptocephali of the eel (Anguilla japonica) to investigate whether or not such organic matter was an appropriate food source for the larvae. A strain was isolated from a biofloc technology system used to culture fish, and seven types of organic material from hen's egg, eel muscle, tuna muscle, lugworm, shrimp, manila clam, mussel, and sea squirt were fermented using isolated bacteria (Bacillus sp.). The fermented matter did not show any specific form and was larger than $10-20{\mu}m$ but no more than $100{\mu}m$ in size. Four diets (A-D) were prepared using the various fermented products, and the larvae were fed the prepared food from 20 days after hatching. The leptocephali fed the A, B, and C diets survived until 37, 39, and 37 days after hatching, respectively. However, the leptocephali fed the D diet survived for 60 days after hatching. The protein content of each diet was very similar, but the n-3 HUFA concentration in the D diet was approximately twice as high as that of the others.

THE TASTE COMPOUNDS FERMENTED ACETES CHINENSIS (새우젓의 정미성분에 관한 연구)

  • CHUNG Seung-Yong;LEE Eung-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.9 no.2
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    • pp.79-110
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    • 1976
  • In Korea fermented fish and shellfish have traditionally been favored and consumed as seasonings or further processed for fish sauce. Three major items in production quantity among more than thirty kinds which are presently available in the market are fermented anchovy, oyster and small shrimp. They are usually used as a seasoning mixture of Kimchi in order to provide a distinctive flavor. Fermented small shrimp, Acetes chinensis is most widely and largely used ana occupies an important position in food industry of this country. But no study on its taste compounds has been reported. This study was attempted to establish the basic data for evaluating taste compounds of fermented small shrimp. The changes of such compounds during fermentation as free amino acids, nucleotides and their related compounds, TMAO, TMA, and betaine were analysed. In addition, change in microflora during the fermentation under the halophilic circumstance was also investigated. The samples were prepared with three different salt contents of 20, 30 and $40\%$ to obtain the proper degree of fermentation at a controlled tempeature of $20{\pm}2^{\circ}C$. The results are summarized as follows: Volatile basic nitrogen increased rapidly until 108 days of fermentation and afterwards it tended to increase slowly. Amino nitrogen also increased rapidly until 43 days of fermentation and then increased slowly. Extract nitrogen increased and marked the maximum value at 72 day fermentation and then decreased slowly. ADP, AMP and IMP tended to degrade rapidly while hypoxanthine increased remarkably at 27 day fermentation but slightly decreased at 72 day fermentation. It is presumed that the characteristic flavor of fermented small shrimp might be attributed to the relatively higher content of hypoxanthine. In the free amino acid composition of fresh small shrimp abundant amino acids were proline, arginine, alanine, glycine, lysine, glutamic acid, leucine, valine and threonine in order. Such amino acids like serine, methionine, isoleucine, phenylalanine, aspartic acid, tyrosine and histidine were poor. In small shrimp extract, proline, arginine, alanine, glycine, lysine and glutamic acid were dominant holding $18.5\%,\;14.6\%,\;10.8\%,\;8.7\%,\;8.1\%\;and\;7.7\%$ of total free amino acids respectively. The total free amino acid nitrogen in fresh small shrimp was $63.9\%$ of its extract nitrogen. The change of free amino acid composition in the extract of small shrimp during fermentation was not observed. Lysine, alanine glutamic acid, proline, glycine and leucine were abundant in both fresh sample and fermented products. The increase of total free amino acids during 72 day fermentation reached approximately more than 2 times as compared with that of fresh sample and then decreased slowly. Fermented small shrimp with $40\%$ of salt was too salty to be commercial quality as the results of organoleptic test showed. It is found that 72 day fermentation with $20\%\;and\;30\%$ of salt gave the most favorable flavor. It is convinced that the characteristic flavor of fermented small shrimp was also attributed to such amino acids as lysine, proline, alanine, glycine and serine known as sweet compounds, as glutamic acid with meaty taste, and as leucine known as bitter taste. The amount of betaine increased during fermentation and reached the maximum at 72 day fermentation and then decreased slowly TMA increased while TMAO decreased during fermentation. The amount of TMAO nitrogen in fermented small shrimp was $200mg\%$ on moisture and salt free base. Betaine and TMAO known as sweet compounds were abundant in fermented small shrimp. It is supposed that these compounds could also play a role as important taste compounds of fermented small shrimp. At the initial stage of fermentation, Achromobacter, Pseudomonas, Micrococcus denitrificans which belong to marine bacteria were isolated. After 40 day fermentation, they disappeared rapidly while Halabacterium, Pediococcus, Sarcian, Micrococcus morrhuae and the yeasts such as Saccharomyces sp. and Torulopsis sp. dominated. It is concluded that the most important taste compounds of fermented small shrimp were amino acids such as lysine, proline, alanine, glycine, serine, glutamic acid, and leucine, betaine, TMAO and hypoxanthine.

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Effect on Fruit Quality and Tree's Main Disease Control by Agro-chemical alternatives (대체농업자재에 의한 과수의 품질 및 주요병해방제 효과)

  • Nam, Ki-Woong;Kim, Seung-Hwan
    • Korean Journal of Organic Agriculture
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    • v.10 no.3
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    • pp.67-77
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    • 2002
  • This study was conducted to investigate an effects on ago-chemical alternative materials such as the wood vinegar, a lactic acid bacteria serum, the fermented plant juice, the brown rice vinegar and a Chitosan used for amount and qualities of fruits and to examine the pest protection efficiency for their uses in the apple and pear orchard farms. An apple yields in the orchard cultivated with using the ago-chemical alternative materials without appling the fertilizer and pesticides were decreased at 56% relative to the conventional farming practice method. Also, it was indicated that there was difficult to produce the fruits with marketability because the small sizes of fruits were produced. For the quality of fruits, the brix of apple produced in the orchard cultivated with using the ago-chemical alternative materials was similar, but Vitamin C content was greater than that of the conventional farming practice method. As a results of treating with the wood vinegar, a lactic acid bacteria serum, the fermented plant juice, the brown rice vinegar and a Chitosan instead of applying pesticides, the fruit disease in the Chitosan treatment was a little decreased, but was great occurred in the other treatments compared with the conventional farming practice mehod. However, it observed that brix and Vitamin C content of apple produced in the Chitosan, brown rice vinegar, fermented plan juice and fish amino acid treatments and in the Chitosan, brown rice vinegar, charcoal power and peat moss treatments were greater than those of the conventional farming practice method, respectively. Over all, it considered that there was very difficult to manage the orchard depended on the ago-chemical alternative materials without appling the chemical fertilizer and pesticides in the apple orchard, but it might be proper to use the ago-chemical alternative materials as an auxiliary means to decrease the appling amount of chemical fertilizer and pesticides. Furthermore, the general effects on the ago-chemical alternative materials to the perennial fruits should be investigated with considering the changes of soil fertility, soil microbial status and natural enemy creatures after treating them for a long time.

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