• CELLMED
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• v.10 no.1
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• pp.7.1-7.6
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• 2020

Plants produce a wide range of active principles, making them a rich source of different types of medicines. Without any knowledge of the phytoconstituents or active principles the medicinal plants itself or in the form of polyherbal formulations, were used by all society of human being from ancient times for prevention and cure of disease, but most of the traditional formulations including the formulation of Ayurveda and Unani have not been scientifically validated in order to establish the pharmacopoeial standards to improve the efficacy. Globally, the people become conscious that uses of synthetic drugs for a long period is not safe; the trend of medical society at large is looking at alternatives from natural, safe sources to combat diseases. Due to this comprehension, it has been increased the demand for plant-derived medicine, and on the other side, it is extremely important to standardize the polyherbal formulations and validate them scientifically to improve their safety and efficacy. The polyherbal Unani formulation Safuf-e-Muallif is widely used and recommended in Unani system of medicine (USM) as a spermatogenic agent, semen thickening agent, etc. to treat sexual disorders viz. premature ejaculation, nocturnal emission, etc. The study mainly deals with phytochemical screening for the detection of nature of phytoconstituents and analytical technique like High-performance thin-layer chromatography (HPTLC) for developing fingerprint of Safuf-e-Muallif revealing specific identities of the drug. The phytochemical screening and HPTLC fingerprint profile for SM reported here may be used as a reference standard for quality control purpose in future.

• Korean Journal of Optics and Photonics
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• v.18 no.6
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• pp.395-400
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• 2007

We propose a design and analysis of an optical system using a prism with a high enough refractive index for wet fingerprint identification. Important parameters including the tilting angle($\beta$) of the $1^{st}$ image plane, an anamorphic distortion, and a tilt of image plane are considered in terms of the apex angle of the prism($\alpha$) and refractive index of the prism material. Our suggestion on refractive index and apex angle of the prism corroborates well with experimental results.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.6
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• pp.589-594
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• 2002

Natural clonal stock of triploid crucian carp, Carassius aurahs was identified and its cytogenetic, molecular genetic and morphological traits were studied. Cytogenetic analysis of the clonal crucian carp revealed that they were natural triploidy, evidenced by 1.5-fold increases of cell size, DNA content, and chromosome number. Multi-locus DNA fingerprinting using $(GATA)_4$ probe showed that they had an identical fingerprint profile, indicating the clonal propagation of the population. External morphology and morphometric characteristics of triploid individuals were much uniform compared to those of diploids. Natural triploid crucian carp was proven to be all-female in this study.

• Journal of Applied Biological Chemistry
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• v.64 no.3
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• pp.277-284
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• 2021

The emergence and rapid spread of the potentially fatal coronavirus disease 2019, caused due to infection by severe acute respiratory syndrome coronavirus-2, has led to worldwide interest in developing functional bioactive ingredients that act as immunomodulatory agents. In this study, we aimed to characterize Carica papaya extract and explore its potential as an immunomodulator by performing in vitro cell screening. Papaya leaf water extract (PLW) was found to significantly increase the levels of nitric oxide (NO) and prostaglandin E₂ (PGE₂) by upregulating inducible nitric oxide synthase and cyclo-oxygenase-2 activity, respectively. Additionally, PLW increased the production of tumor necrosis factor-α and interleukin 1β in RAW 264.7 cells. Furthermore, PLW activated the expression of c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) but not that of p38 mitogen-activated protein kinase. These results indicate that PLW increased the production of NO, PGE₂, and pro-inflammatory cytokines by activating the JNK and ERK pathways in macrophages, thus demonstrating immunomodulatory properties. Finally, high-performance liquid chromatography fingerprint analysis indicated the presence of rutin, narirutin, and ρ-coumaric acid in PLW (6.30, 119.76, and 47.25 ppm, respectively). Treating cells with these compounds at non-toxic concentrations had no effect on NO production. Taken together, these results suggest that PLW may have potential as an immunity-enhancing supplement.

• Analytical Science and Technology
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• v.34 no.2
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• pp.56-67
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• 2021

Due to the difficulty of grading visualized fingerprints with previously known evaluation methods for the comparison of split fingerprints, a preliminary study was performed with the densitometric image analysis (DIA) method as a potential quantitative and supplementary evaluation method. Each image of inked split fingerprints was divided into 4 zones for analysis. Weekly intra- and inter- analysis by two analysts with three whole fingerprints that were constructed by combining inked split fingerprints showed that the average area values and the ranges of difference fluctuation were not significantly different between strong fingerprints and strong-weak pairs, while they were different in weak fingerprints and weak-weak pairs. In the case of weak fingerprints, the exact acquisition of ridges was difficult and this seemed to influence the results. An additional study is needed for the improved reliability using DIA method with weak fingerprints such as 8 zones division rather than 4 zones. In addition, the analysis results performed by several analysts at different times should be used to improve the reliability of the analysis method further. Based on the above result, it can be judged that utilizing the DIA method as a secondary evaluation method of the existing scoring system would be effective with the additional studies especially on weak fingerprints.

• The Journal of Korean Medicine
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• v.20 no.4
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• pp.62-68
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• 2000

This study was carried out to establish genetic understanding of three Sasang constitutions of Taeumin, Soeumin and Soyangin by Random Amplified Polymorphic DNA(RAPD) analysis. We have applied RAPD analysis to pooled DNA sample as a means to achieve rapid screening of large numbers of primers for their capacity to reveal constitutions-specific polymorphisms. From an initial 440 primers, 13 polymorphic primers between different constitutions were selected. Bandsharing(BS) and mean average percentage difference(MAPD) calculated within and between three constitutions using RAPD fingerprint data showed a higher degree of homogenity within than between the constitutions and indicated measurable divergence between three constitutions. The RAPD bandsharing(BS) values ranged from 0.71 to 0.73 between the three constitutions. The interconstitution divergence was narrower between Taeumin and Soeumin, than between the other paired constitution comparisons. The genetic distance between the three constitutions was measured by BS values. Genetic distance by RAPD analysis was 0.007 between Taeumin and Soeumin, and 0.014 between Soyang and the others. In conclusion, the genetic distance of Teaumin and Soumin was closer than that of Soyangin in the analysis of RAPD by using 440 primers.

• Mass Spectrometry Letters
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• v.12 no.3
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• pp.93-105
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• 2021

Exosomes have gained the attention of the scientific community because of their role in facilitating intercellular communication, which is critical in disease monitoring and drug delivery research. Exosome research has grown significantly in recent decades, with a focus on the development of various technologies for isolating and characterizing exosomes. Among these efforts is the use of matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS), which offers high-throughput direct analysis while also being cost and time effective. MALDI is used less frequently in exosome research than electrospray ionization due to the diverse population of extracellular vesicles and the impurity of isolated products, both of which necessitate chromatographic separation prior to MS analysis. However, MALDI-MS is a more appropriate instrument for the analytical approach to patient therapy, given it allows for fast and label-free analysis. There is a huge drive to explore MALDI-MS in exosome research because the technology holds great potential, most notably in biomarker discovery. With methods such as fingerprint analysis, OMICs profiling, and statistical analysis, the search for biomarkers could be much more efficient. In this review, we highlight the potential of MALDI-MS as a tool for investigating exosomes and some of the possible strategies that can be implemented based on prior research.

• Mass Spectrometry Letters
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• v.13 no.4
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• pp.106-114
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• 2022

A rapid and reliable approach to the identification of microorganisms is a critical requirement for large-scale culturomics analysis. MALDI-TOF MS is a suitable technique that can be a better alternative to conventional biochemical and gene sequencing methods as it is economical both in terms of cost and labor. In this review, the applications of MALDI-TOF MS for the comprehensive identification of microorganisms and bacterial strain typing for culturomics-based approaches for various environmental studies including bioremediation, plant sciences, agriculture and food microbiology have been widely explored. However, the restriction of this technique is attributed to insufficient coverage of the mass spectral database. To improve the applications of this technique for the identification of novel isolates, the spectral database should be updated with the peptide mass fingerprint (PMF) of type strains with not only microbes with clinical relevance but also from various environmental sources. Further, the development of enhanced sample processing methods and new algorithms for automation and de-replication of isolates will increase its application in microbial ecology studies.

• Journal of the Korean Society for Marine Environment & Energy
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• v.7 no.3
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• pp.122-130
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• 2004

Crude oil is complex mixture of thousands of different organic compound formed from a variety of organic materials that are chemically converted under differing geological conditions over long periods of time. Also oil composition varies according to crude source, refining, processing, handling and storage. The oil fingerprint method is application of specific knowledge of petrochemicals and use of sophisticated analytical equipment and techniques to identify the source(s) of oil pollution. KNMPA currently utilizes three primary analytical techniques: Gas Chromatography (GC), Fluorescence Spectroscopy(FL) and Infrared Spectroscopy(IR). Of all these techniques, GC technique are most widely used. Gas Chromatography is used as a primary analytical method because high reliableness, high separating efficiency and repeatability, but it is timeconsumable. The study results of identification of waterborne spilled oil by Fast Gas Chromatograph method showed that analytical time is cut down to 30minutes in comparison with packed column method and chromatograms represent high resolution and high repeatability.

• Horticultural Science & Technology
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• v.29 no.3
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• pp.240-246
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• 2011

The principal objective of this study was to estimate the availability of morphological data on the basis of the guidelines of the International Union for the Protection of New Varieties of Plants (UPOV) with regard to the identification of the rose germplasm. The correlation of morphological traits and random amplified polymorphic DNA (RAPD) marker data among 44 rose cultivars was assessed via a mantel test. Thirty eight phenotypes were employed for morphological analysis. Sixteen primers were utilized for RAPD analysis, and these generated 225 polymorphic bands. The dendrogram based on the RAPD markersgrouped 44 rose cultivars according to their horticultural types. No significant correlation was observed between the morphological and RAPD marker data. We concluded that current UPOV traits could not be applied to study genetic variation. Further studies on morphological traits are required for the analysis of genetic variation among cultivars.