• 제목/요약/키워드: Fine Mapping

검색결과 128건 처리시간 0.033초

Detection of QTL for Carcass Quality on Chromosome 6 by Exploiting Linkage and Linkage Disequilibrium in Hanwoo

  • Lee, J.H.;Li, Y.;Kim, J.J.
    • Asian-Australasian Journal of Animal Sciences
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    • 제25권1호
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    • pp.17-21
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    • 2012
  • The purpose of this study was to improve mapping power and resolution for the QTL influencing carcass quality in Hanwoo, which was previously detected on the bovine chromosome (BTA) 6. A sample of 427 steers were chosen, which were the progeny from 45 Korean proven sires in the Hanwoo Improvement Center, Seosan, Korea. The samples were genotyped with the set of 2,535 SNPs on BTA6 that were imbedded in the Illumina bovine 50 k chip. A linkage disequilibrium variance component mapping (LDVCM) method, which exploited both linkage between sires and their steers and population-wide linkage disequilibrium, was applied to detect QTL for four carcass quality traits. Fifteen QTL were detected at 0.1% comparison-wise level, for which five, three, five, and two QTL were associated with carcass weight (CWT), backfat thickness (BFT), longissimus dorsi muscle area (LMA), and marbling score (Marb), respectively. The number of QTL was greater compared with our previous results, in which twelve QTL for carcass quality were detected on the BTA6 in the same population by applying other linkage disequilibrium mapping approaches. One QTL for LMA was detected on the distal region (110,285,672 to 110,633,096 bp) with the most significant evidence for linkage (p< $10^{-5}$). Another QTL that was detected on the proximal region (33,596,515 to 33,897,434 bp) was pleiotrophic, i.e. influencing CWT, BFT, and LMA. Our results suggest that the LDVCM is a good alternative method for QTL fine-mapping in detection and characterization of QTL.

Identification of Candidate Genes Associated with Beef Marbling Using QTL and Pathway Analysis in Hanwoo (Korean Cattle)

  • Park, Hye-Sun;Seo, Seong-Won;Cho, Yong-Min;Oh, Sung-Jong;Seong, Hwan-Hoo;Lee, Seung-Hwan;Lim, Da-Jeong
    • Asian-Australasian Journal of Animal Sciences
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    • 제25권5호
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    • pp.613-620
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    • 2012
  • Marbling from intramuscular fat is an important trait of meat quality and has an economic benefit for the beef industry. Quantitative trait loci (QTL) fine mapping was performed to identify the marbling trait in 266 Hanwoo steers using a 10K single nucleotide polymorphism panel with the combined linkage and linkage disequilibrium method. As a result, we found nine putative QTL regions for marbling: three on BTA6, two on BTA17, two on BTA22, and two on BTA29. We detected candidate genes for marbling within 1 cM of either side of the putative QTL regions. Additionally, to understand the functions of these candidate genes at the molecular level, we conducted a functional categorization using gene ontology and pathway analyses for those genes involved in lipid metabolism or fat deposition. In these putative QTL regions, we found 95 candidate genes for marbling. Using these candidate genes, we found five genes that had a direct interaction with the candidate genes. We also found SCARB1 as a putative candidate gene for marbling that involves fat deposition related to cholesterol transport.

Investigation of Urban Environmental Quality Using an Integration of Satellite, Ground based measurement data over Seoul, Korea

  • Lee, Kwon-Ho;Wong, Man-Sing;Kim, Young-J.
    • 대한원격탐사학회지
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    • 제27권3호
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    • pp.339-351
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    • 2011
  • This study investigates the potentials of satellite, ground measurement data, and geo-spatial information within an urban area for the mapping of the Urban Environmental Quality (UEQ) parameters. The UEQ indicates a complex and various parameters resulting from both human and natural factors, which are greenness, climate, air pollution, the urban infrastructure, and etc. Multi-spectral remote sensing data from the Landsat ETM and TM sensors for the mapping of air pollution by the Haze Optimized Transform (HOT) technique, Urban Heat Island (UHO using the emissivity-fusion method in Seoul from 2000 to 2006 in fine resolution (30m) were analyzed for the estimation of UEQ index. Although the UHI values are similar ($8.4^{\circ}C{\sim}9.1^{\circ}C$) during these years, the spatial coverage of "hot" surface temperature (> $24^{\circ}C$) significantly increased from 2000 to 2006 due to the rapid urban development. Furthermore, high correlations between vegetation index and land surface temperature were achieved with a correlation coefficients of 0.85 (2000), 0.81 (2001), 0.84 (2002), and 0.89 (2006), respectively. It was found that the proposed method was successfully analyzed spatial structure of the UEQ and the scenarios of the best and worst areas within the city were also identified. Based on the quantifiable fine resolution satellite image parameters, UEQ can promote the understanding of the complex and dynamic factors controlling urban environment.

남자대학생들의 각막 형태 연구 (The morphological study of Cornea in Man College Students)

  • 김덕훈;유재태
    • 한국안광학회지
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    • 제7권2호
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    • pp.89-93
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    • 2002
  • 각막의 생리와 형태는 안 굴절에 영향을 미친다. 그래서 케라토메터에 의한 전방 각막 표면의 측정은 안경사, 검안사 그리고 안과의사에 의해 안경과 콘택트렌즈의 처방에 사용된다. 더욱이 현재 대게 각막 지도 체제는 임상 안경원, 안굴절 센타, 그리고 안과병원에서 사용되어진다. 왜냐하면 이 방법은 이전 도구에 비해서 매우 좋은 자료를 가진다. 저자들은 각막 지도 방법에 의해 남자 대학생들의 각막을 측정하였다. 더욱이 이 자료들은 콘택트렌즈를 위한 임상 실제에서 응용되어질 것이다. 특히 한국에서 많은 남자 대학생들은 시력과 미용을 위해 콘택트렌즈 사용을 한다. 따라서 콘택트렌즈 착용자들은 매년 증가되고 있다.

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이종 개념체계의 상호보완방안 연구 - 세종의미부류와 KorLexNoun 1.5 의 사상을 중심으로 (Cross-Enrichment of the Heterogenous Ontologies Through Mapping Their Conceptual Structures: the Case of Sejong Semantic Classes and KorLexNoun 1.5)

  • 배선미;윤애선
    • 한국언어정보학회지:언어와정보
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    • 제14권1호
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    • pp.165-196
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    • 2010
  • The primary goal of this paper is to propose methods of enriching two heterogeneous ontologies: Sejong Semantic Classes (SJSC) and KorLexNoun 1.5 (KLN). In order to achieve this goal, this study introduces the pros and cons of two ontologies, and analyzes the error patterns found during the fine-grained manual mapping processes between them. Error patterns can be classified into four types: (1) structural defectives involved in node branching, (2) errors in assigning the semantic classes, (3) deficiency in providing linguistic information, and (4) lack of the lexical units representing specific concepts. According to these error patterns, we propose different solutions in order to correct the node branching defectives and the semantic class assignment, to complement the deficiency of linguistic information, and to increase the number of lexical units suitably allotted to their corresponding concepts. Using the results of this study, we can obtain more enriched ontologies by correcting the defects and errors in each ontology, which will lead to the enhancement of practicality for syntactic and semantic analysis.

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Gabor 웨이브렛과 FCM 군집화 알고리즘에 기반한 동적 연결모형에 의한 얼굴표정에서 특징점 추출 (Feature-Point Extraction by Dynamic Linking Model bas Wavelets and Fuzzy C-Means Clustering Algorithm)

  • 신영숙
    • 인지과학
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    • 제14권1호
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    • pp.11-16
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    • 2003
  • 본 논문은 Gabor 웨이브렛 변환을 이용하여 무표정을 포함한 표정영상에서 얼굴의 주요 요소들의 경계선을 추출한 후, FCM 군집화 알고리즘을 적용하여 무표정 영상에서 저차원의 대표적인 특징점을 추출한다. 무표정 영상의 특징점들은 표정영상의 특징점들을 추출하기 위한 템플릿으로 사용되어지며, 표정영상의 특징점 추출은 무표정 영상의 특징점과 동적 연결모형을 이용하여 개략적인 정합과 정밀한 정합 과정의 두단계로 이루어진다. 본 논문에서는 Gabor 웨이브렛과 FCM 군집화 알고리즘을 기반으로 동적 연결모형을 이용하여 표정영상에서 특징점들을 자동으로 추출할 수 있음을 제시한다. 본 연구결과는 자동 특징추출을 이용한 차원모형기반 얼굴 표정인식[1]에서 얼굴표정의 특징점을 자동으로 추출하는 데 적용되었다.

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인간언어공학에의 활용을 위한 이종 개념체계 간 사상 -세종의미부류와 KorLexNoun 1.5- (Mapping Heterogenous Hierarchical Concept Classifications for the HLP Applications -A case of Sejong Semantic Classes and KorLexNoun 1.5-)

  • 배선미;임경업;윤애선
    • 한국정보과학회 언어공학연구회:학술대회논문집(한글 및 한국어 정보처리)
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    • 한국정보과학회언어공학연구회 2009년도 제21회 한글 및 한국어 정보처리 학술대회
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    • pp.6-13
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    • 2009
  • 본 연구에서는 인간언어공학에서의 활용을 위해 세종전자사전의 의미부류와 KorLexNoun 1.5의 상위노드 간의 사상을 목표로 전문가의 수작업에 의한 세밀한 사상 방법론(fine-grained mapping method)을 제안한다. 또한 이질적인 두 이종 자원 간의 사상에 있어 각 의미체계의 이질성으로 인해 발생하는 여러 가지 문제점을 살펴보고, 그 해결방안을 제안한다. 본 연구는 세종의미부류체계가 밝히고자 했던 한국어의 의미구조와, Prinston WordNet을 참조로 하여 KorLexNoun에 여전히 영향을 미치고 있는 영어 의미구조를 비교함으로써 공통점과 차이점을 파악할 수 있고, 이를 바탕으로 언어 독립적인 개념체계를 구축하는 데 기여할 수 있다. 또한 향후 KorLex의 용언에 기술되어 있는 문형정보와 세종 전자사전의 용언의 격틀 정보를 통합 구축하여 구문분석에서 이용할 때, 세종 의미부류와 KorLexNoun의 상위노드를 통합 구축함으로써 논항의 일반화된 선택제약규칙의 기술에서 이용될 수 있다. 본 연구에서 제안된 사상방법론은 향후 이종 자원의 자동 사상 연구에서도 크게 기여할 것이다. 아울러 두 이종 자원의 사상을 통해 두 의미체계가 지닌 장점을 극대화하고, 동시에 단점을 상호 보완하여 보다 완전한 언어자원으로써 구문분석이나 의미분석에서 이용될 수 있다.

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Fine Mapping of the Rice Bph1 Gene, which Confers Resistance to the Brown Planthopper (Nilaparvata lugens Stal), and Development of STS Markers for Marker-assisted Selection

  • Cha, Young-Soon;Ji, Hyeonso;Yun, Doh-Won;Ahn, Byoung-Ohg;Lee, Myung Chul;Suh, Seok-Cheol;Lee, Chun Seok;Ahn, Eok Keun;Jeon, Yong-Hee;Jin, Il-Doo;Sohn, Jae-Keun;Koh, Hee-Jong;Eun, Moo-Young
    • Molecules and Cells
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    • 제26권2호
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    • pp.146-151
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    • 2008
  • The brown planthopper (BPH) is a major insect pest in rice, and damages these plants by sucking phloem-sap and transmitting viral diseases. Many BPH resistance genes have been identified in indica varieties and wild rice accessions, but none has yet been cloned. In the present study we report fine mapping of the region containing the Bph1 locus, which enabled us to perform marker-aided selection (MAS). We used 273 F8 recombinant inbred lines (RILs) derived from a cross between Cheongcheongbyeo, an indica type variety harboring Bph1 from Mudgo, and Hwayeongbyeo, a BPH susceptible japonica variety. By random amplification of polymorphic DNA (RAPD) analysis using 656 random 10-mer primers, three RAPD markers (OPH09, OPA10 and OPA15) linked to Bph1 were identified and converted to SCAR (sequence characterized amplified region) markers. These markers were found to be contained in two BAC clones derived from chromosome 12: OPH09 on OSJNBa0011B18, and both OPA10 and OPA15 on OSJNBa0040E10. By sequence analysis of ten additional BAC clones evenly distributed between OSJNBa0011B18 and OSJNBa0040E10, we developed 15 STS markers. Of these, pBPH4 and pBPH14 flanked Bph1 at distances of 0.2 cM and 0.8 cM, respectively. The STS markers pBPH9, pBPH19, pBPH20, and pBPH21 co-segregated with Bph1. These markers were shown to be very useful for marker-assisted selection (MAS) in breeding populations of 32 F6 RILs from a cross between Andabyeo and IR71190, and 32 F5 RILs from a cross between Andabyeo and Suwon452.

Application of genomics into rice breeding

  • Ando, Ikuo
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.13-13
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    • 2017
  • By the progress of genome sequencing, infrastructures for marker-assisted breeding (MAB) of rice came to be established. Fine mapping and gene isolation have been conducted using the breeding materials derived from natural variations and artificial mutants. Such genetic analysis by the genome-wide dense markers provided us the knowledge about the many genes controlling important traits. We identified several genes or quantitative trait loci (QTL) for heading date, blast resistance, eating quality, high-temperature stress tolerance, and so on. NILs of each gene controlling heading date contribute to elongate the rice harvest period. Determination of precise gene location of blast resistance gene pi21, allowed us to overcome linkage drag, co-introduction of undesirable eating quality. We could also breed the first practical rice cultivar in Japan with a brown planthopper resistance gene bph11 in the genetic back-ground of an elite cultivar. Discovery of major and minor QTLs for good eating quality allowed us to fine-tune of eating quality according to the rice planting area or usage of rice grain. Many rice cultivars have bred efficiently by MAB for several traits, or by marker-assisted backcross breeding through chromosome segment substitution lines (CSSLs) using genetically diverse accessions. We are also systematically supporting the crop breeding of other sectors by MAB or by providing resources such as CSSLs. It is possible to pyramid many genes for important traits by using MAB, but is still difficult to improve the yielding ability. We are performing a Genomic Selection (GS) for improvement of rice biomass and grain yield. We are also trying to apply the genome editing technology for high yield rice breeding.

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저해상도 비율 자료로부터 고해상도 범주형 주제도 생성을 위한 지구통계학적 블록 시뮬레이션 (A Geostatistical Block Simulation Approach for Generating Fine-scale Categorical Thematic Maps from Coarse-scale Fraction Data)

  • 박노욱;이기원
    • 한국지구과학회지
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    • 제32권6호
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    • pp.525-536
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    • 2011
  • 다양한 형태의 공간 자료를 이용하는 응용 분야에서 이용가능한 자료간 해상도 차이를 반영하고, 원하는 타겟 해상도로의 변환은 매우 중요하다. 이 논문에서는 저해상도 비율 자료로부터 고해상도 범주형 주제도를 제작하기 위해 베리오그램 디컨볼루션과 블록 시뮬레이션을 이용하는 지구통계학적 다운스케일링 방법론을 제시하였다. 우선 블록 기반 베리오그램으로부터 점 기반 베리오그램 모델의 추정을 위해 반복 연산 기반 베리오그램 디컨볼루션 방법을 적용하였다. 그리고 영역-점 변환 크리깅과 추정된 점 기반 베리오그램 모델을 이용하는 직접 순차적 시뮬레이션을 적용하여 고해상도 비율 정보를 제작하였다. 최종적으로 최대 사후 확률 결정 규칙을 적용하여 대안적 고해상도 범주형 주제도를 제작하였다. 점 기반 자료 없이 저해상도 블록 비율자료만을 이용한 토지피복도 제작 사례 연구를 통해 제안 분석 과정을 예시하였다. 제안한 다운스케일링 기법의 적용을 통해 원 저해상도 블록 비율 자료값을 재생산하는 고해상도 비율 자료를 생성하였으며, 이로부터 기준 토지피복도의 전반적인 패턴을 반영하는 대안적 고해상도 토지피복도 제작이 가능함을 확인할 수 있었다. 이렇게 생성된 고해상도 자료들은 해상도 변환의 영향 분석에 유용한 입력 자료로 이용될 수 있을 것으로 생각된다.