• Title/Summary/Keyword: Fine Mapping

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Detection of QTL for Carcass Quality on Chromosome 6 by Exploiting Linkage and Linkage Disequilibrium in Hanwoo

  • Lee, J.H.;Li, Y.;Kim, J.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.1
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    • pp.17-21
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    • 2012
  • The purpose of this study was to improve mapping power and resolution for the QTL influencing carcass quality in Hanwoo, which was previously detected on the bovine chromosome (BTA) 6. A sample of 427 steers were chosen, which were the progeny from 45 Korean proven sires in the Hanwoo Improvement Center, Seosan, Korea. The samples were genotyped with the set of 2,535 SNPs on BTA6 that were imbedded in the Illumina bovine 50 k chip. A linkage disequilibrium variance component mapping (LDVCM) method, which exploited both linkage between sires and their steers and population-wide linkage disequilibrium, was applied to detect QTL for four carcass quality traits. Fifteen QTL were detected at 0.1% comparison-wise level, for which five, three, five, and two QTL were associated with carcass weight (CWT), backfat thickness (BFT), longissimus dorsi muscle area (LMA), and marbling score (Marb), respectively. The number of QTL was greater compared with our previous results, in which twelve QTL for carcass quality were detected on the BTA6 in the same population by applying other linkage disequilibrium mapping approaches. One QTL for LMA was detected on the distal region (110,285,672 to 110,633,096 bp) with the most significant evidence for linkage (p< $10^{-5}$). Another QTL that was detected on the proximal region (33,596,515 to 33,897,434 bp) was pleiotrophic, i.e. influencing CWT, BFT, and LMA. Our results suggest that the LDVCM is a good alternative method for QTL fine-mapping in detection and characterization of QTL.

Identification of Candidate Genes Associated with Beef Marbling Using QTL and Pathway Analysis in Hanwoo (Korean Cattle)

  • Park, Hye-Sun;Seo, Seong-Won;Cho, Yong-Min;Oh, Sung-Jong;Seong, Hwan-Hoo;Lee, Seung-Hwan;Lim, Da-Jeong
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.5
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    • pp.613-620
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    • 2012
  • Marbling from intramuscular fat is an important trait of meat quality and has an economic benefit for the beef industry. Quantitative trait loci (QTL) fine mapping was performed to identify the marbling trait in 266 Hanwoo steers using a 10K single nucleotide polymorphism panel with the combined linkage and linkage disequilibrium method. As a result, we found nine putative QTL regions for marbling: three on BTA6, two on BTA17, two on BTA22, and two on BTA29. We detected candidate genes for marbling within 1 cM of either side of the putative QTL regions. Additionally, to understand the functions of these candidate genes at the molecular level, we conducted a functional categorization using gene ontology and pathway analyses for those genes involved in lipid metabolism or fat deposition. In these putative QTL regions, we found 95 candidate genes for marbling. Using these candidate genes, we found five genes that had a direct interaction with the candidate genes. We also found SCARB1 as a putative candidate gene for marbling that involves fat deposition related to cholesterol transport.

Investigation of Urban Environmental Quality Using an Integration of Satellite, Ground based measurement data over Seoul, Korea

  • Lee, Kwon-Ho;Wong, Man-Sing;Kim, Young-J.
    • Korean Journal of Remote Sensing
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    • v.27 no.3
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    • pp.339-351
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    • 2011
  • This study investigates the potentials of satellite, ground measurement data, and geo-spatial information within an urban area for the mapping of the Urban Environmental Quality (UEQ) parameters. The UEQ indicates a complex and various parameters resulting from both human and natural factors, which are greenness, climate, air pollution, the urban infrastructure, and etc. Multi-spectral remote sensing data from the Landsat ETM and TM sensors for the mapping of air pollution by the Haze Optimized Transform (HOT) technique, Urban Heat Island (UHO using the emissivity-fusion method in Seoul from 2000 to 2006 in fine resolution (30m) were analyzed for the estimation of UEQ index. Although the UHI values are similar ($8.4^{\circ}C{\sim}9.1^{\circ}C$) during these years, the spatial coverage of "hot" surface temperature (> $24^{\circ}C$) significantly increased from 2000 to 2006 due to the rapid urban development. Furthermore, high correlations between vegetation index and land surface temperature were achieved with a correlation coefficients of 0.85 (2000), 0.81 (2001), 0.84 (2002), and 0.89 (2006), respectively. It was found that the proposed method was successfully analyzed spatial structure of the UEQ and the scenarios of the best and worst areas within the city were also identified. Based on the quantifiable fine resolution satellite image parameters, UEQ can promote the understanding of the complex and dynamic factors controlling urban environment.

The morphological study of Cornea in Man College Students (남자대학생들의 각막 형태 연구)

  • Kim, Douk Hoon;Yoo, Jae Tae
    • Journal of Korean Ophthalmic Optics Society
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    • v.7 no.2
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    • pp.89-93
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    • 2002
  • The physiology and morphology of cornea has effected on the ocular refractive status. Therefore the measurement of the anterior corneal surface by keratometer is used the treatment of the spectacles and contact lens by optician, optometrist, and ophthalmologist. Furthermore, currently the most corneal topographic mapping system have used the clinical optical shop, eye research center, and eye hospital. Because this method have a very fine data comparative to the previous instrument. The authors have measurement the cornea of the man college students by the corneal topographic mapping method. Especially this data will applied the clinical practice for the contact lens fitting. Especially in Korea, many man college students use the contact lens for the visual acuity and a beauty. Therefore the contact lens wear have increase per every year.

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Cross-Enrichment of the Heterogenous Ontologies Through Mapping Their Conceptual Structures: the Case of Sejong Semantic Classes and KorLexNoun 1.5 (이종 개념체계의 상호보완방안 연구 - 세종의미부류와 KorLexNoun 1.5 의 사상을 중심으로)

  • Bae, Sun-Mee;Yoon, Ae-Sun
    • Language and Information
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    • v.14 no.1
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    • pp.165-196
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    • 2010
  • The primary goal of this paper is to propose methods of enriching two heterogeneous ontologies: Sejong Semantic Classes (SJSC) and KorLexNoun 1.5 (KLN). In order to achieve this goal, this study introduces the pros and cons of two ontologies, and analyzes the error patterns found during the fine-grained manual mapping processes between them. Error patterns can be classified into four types: (1) structural defectives involved in node branching, (2) errors in assigning the semantic classes, (3) deficiency in providing linguistic information, and (4) lack of the lexical units representing specific concepts. According to these error patterns, we propose different solutions in order to correct the node branching defectives and the semantic class assignment, to complement the deficiency of linguistic information, and to increase the number of lexical units suitably allotted to their corresponding concepts. Using the results of this study, we can obtain more enriched ontologies by correcting the defects and errors in each ontology, which will lead to the enhancement of practicality for syntactic and semantic analysis.

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Feature-Point Extraction by Dynamic Linking Model bas Wavelets and Fuzzy C-Means Clustering Algorithm (Gabor 웨이브렛과 FCM 군집화 알고리즘에 기반한 동적 연결모형에 의한 얼굴표정에서 특징점 추출)

  • 신영숙
    • Korean Journal of Cognitive Science
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    • v.14 no.1
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    • pp.11-16
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    • 2003
  • This Paper extracts the edge of main components of face with Gator wavelets transformation in facial expression images. FCM(Fuzzy C-Means) clustering algorithm then extracts the representative feature points of low dimensionality from the edge extracted in neutral face. The feature-points of the neutral face is used as a template to extract the feature-points of facial expression images. To match point to Point feature points on an expression face against each feature point on a neutral face, it consists of two steps using a dynamic linking model, which are called the coarse mapping and the fine mapping. This paper presents an automatic extraction of feature-points by dynamic linking model based on Gabor wavelets and fuzzy C-means(FCM) algorithm. The result of this study was applied to extract features automatically in facial expression recognition based on dimension[1].

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Mapping Heterogenous Hierarchical Concept Classifications for the HLP Applications -A case of Sejong Semantic Classes and KorLexNoun 1.5- (인간언어공학에의 활용을 위한 이종 개념체계 간 사상 -세종의미부류와 KorLexNoun 1.5-)

  • Bae, Sun-Mee;Im, Kyoungup;Yoon, Aesun
    • Annual Conference on Human and Language Technology
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    • 2009.10a
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    • pp.6-13
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    • 2009
  • 본 연구에서는 인간언어공학에서의 활용을 위해 세종전자사전의 의미부류와 KorLexNoun 1.5의 상위노드 간의 사상을 목표로 전문가의 수작업에 의한 세밀한 사상 방법론(fine-grained mapping method)을 제안한다. 또한 이질적인 두 이종 자원 간의 사상에 있어 각 의미체계의 이질성으로 인해 발생하는 여러 가지 문제점을 살펴보고, 그 해결방안을 제안한다. 본 연구는 세종의미부류체계가 밝히고자 했던 한국어의 의미구조와, Prinston WordNet을 참조로 하여 KorLexNoun에 여전히 영향을 미치고 있는 영어 의미구조를 비교함으로써 공통점과 차이점을 파악할 수 있고, 이를 바탕으로 언어 독립적인 개념체계를 구축하는 데 기여할 수 있다. 또한 향후 KorLex의 용언에 기술되어 있는 문형정보와 세종 전자사전의 용언의 격틀 정보를 통합 구축하여 구문분석에서 이용할 때, 세종 의미부류와 KorLexNoun의 상위노드를 통합 구축함으로써 논항의 일반화된 선택제약규칙의 기술에서 이용될 수 있다. 본 연구에서 제안된 사상방법론은 향후 이종 자원의 자동 사상 연구에서도 크게 기여할 것이다. 아울러 두 이종 자원의 사상을 통해 두 의미체계가 지닌 장점을 극대화하고, 동시에 단점을 상호 보완하여 보다 완전한 언어자원으로써 구문분석이나 의미분석에서 이용될 수 있다.

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Fine Mapping of the Rice Bph1 Gene, which Confers Resistance to the Brown Planthopper (Nilaparvata lugens Stal), and Development of STS Markers for Marker-assisted Selection

  • Cha, Young-Soon;Ji, Hyeonso;Yun, Doh-Won;Ahn, Byoung-Ohg;Lee, Myung Chul;Suh, Seok-Cheol;Lee, Chun Seok;Ahn, Eok Keun;Jeon, Yong-Hee;Jin, Il-Doo;Sohn, Jae-Keun;Koh, Hee-Jong;Eun, Moo-Young
    • Molecules and Cells
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    • v.26 no.2
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    • pp.146-151
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    • 2008
  • The brown planthopper (BPH) is a major insect pest in rice, and damages these plants by sucking phloem-sap and transmitting viral diseases. Many BPH resistance genes have been identified in indica varieties and wild rice accessions, but none has yet been cloned. In the present study we report fine mapping of the region containing the Bph1 locus, which enabled us to perform marker-aided selection (MAS). We used 273 F8 recombinant inbred lines (RILs) derived from a cross between Cheongcheongbyeo, an indica type variety harboring Bph1 from Mudgo, and Hwayeongbyeo, a BPH susceptible japonica variety. By random amplification of polymorphic DNA (RAPD) analysis using 656 random 10-mer primers, three RAPD markers (OPH09, OPA10 and OPA15) linked to Bph1 were identified and converted to SCAR (sequence characterized amplified region) markers. These markers were found to be contained in two BAC clones derived from chromosome 12: OPH09 on OSJNBa0011B18, and both OPA10 and OPA15 on OSJNBa0040E10. By sequence analysis of ten additional BAC clones evenly distributed between OSJNBa0011B18 and OSJNBa0040E10, we developed 15 STS markers. Of these, pBPH4 and pBPH14 flanked Bph1 at distances of 0.2 cM and 0.8 cM, respectively. The STS markers pBPH9, pBPH19, pBPH20, and pBPH21 co-segregated with Bph1. These markers were shown to be very useful for marker-assisted selection (MAS) in breeding populations of 32 F6 RILs from a cross between Andabyeo and IR71190, and 32 F5 RILs from a cross between Andabyeo and Suwon452.

Application of genomics into rice breeding

  • Ando, Ikuo
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.13-13
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    • 2017
  • By the progress of genome sequencing, infrastructures for marker-assisted breeding (MAB) of rice came to be established. Fine mapping and gene isolation have been conducted using the breeding materials derived from natural variations and artificial mutants. Such genetic analysis by the genome-wide dense markers provided us the knowledge about the many genes controlling important traits. We identified several genes or quantitative trait loci (QTL) for heading date, blast resistance, eating quality, high-temperature stress tolerance, and so on. NILs of each gene controlling heading date contribute to elongate the rice harvest period. Determination of precise gene location of blast resistance gene pi21, allowed us to overcome linkage drag, co-introduction of undesirable eating quality. We could also breed the first practical rice cultivar in Japan with a brown planthopper resistance gene bph11 in the genetic back-ground of an elite cultivar. Discovery of major and minor QTLs for good eating quality allowed us to fine-tune of eating quality according to the rice planting area or usage of rice grain. Many rice cultivars have bred efficiently by MAB for several traits, or by marker-assisted backcross breeding through chromosome segment substitution lines (CSSLs) using genetically diverse accessions. We are also systematically supporting the crop breeding of other sectors by MAB or by providing resources such as CSSLs. It is possible to pyramid many genes for important traits by using MAB, but is still difficult to improve the yielding ability. We are performing a Genomic Selection (GS) for improvement of rice biomass and grain yield. We are also trying to apply the genome editing technology for high yield rice breeding.

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A Geostatistical Block Simulation Approach for Generating Fine-scale Categorical Thematic Maps from Coarse-scale Fraction Data (저해상도 비율 자료로부터 고해상도 범주형 주제도 생성을 위한 지구통계학적 블록 시뮬레이션)

  • Park, No-Wook;Lee, Ki-Won
    • Journal of the Korean earth science society
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    • v.32 no.6
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    • pp.525-536
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    • 2011
  • In any applications using various types of spatial data, it is very important to account for the scale differences among available data sets and to change the scale to the target one as well. In this paper, we propose to use a geostatistical downscaling approach based on vaiorgram deconvloution and block simulation to generate fine-scale categorical thematic maps from coarse-scale fraction data. First, an iterative variogram deconvolution method is applied to estimate a point-support variogram model from a block-support variogram model. Then, both a direct sequential simulation based on area-to-point kriging and the estimated point-support variogram are applied to produce alternative fine-scale fraction realizations. Finally, a maximum a posteriori decision rule is applied to generate the fine-scale categorical thematic maps. These analytical steps are illustrated through a case study of land-cover mapping only using the block fraction data of thematic classes without point data. Alternative fine-scale fraction maps by the downscaling method presented in this study reproduce the coarse-scale block fraction values. The final fine-scale land-cover realizations can reflect overall spatial patterns of the reference land-cover map, thus providing reasonable inputs for the impact assessment in change of support problems.