• 소성∙가공
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• 제20권3호
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• pp.250-256
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• 2011

Vacuum-assisted thermoforming is one of the critical steps for the successful application of film insert molding(FIM) to parts of complex shapes. If the thickness distribution of the formed film is non-uniform, cracking, deformation, warping, and wrinkling can easily occur at the injection molding stage. In this study, the effects of processing parameters, which include the film heating time, plug depth, plug speed and vacuum delay time, on film thickness distribution were investigated. It was found that the film thickness at the part sidewall decreases with increasing the film heating time and plug depth, but the thickness at the bottom was found to exhibit the opposite behavior. The film thickness of the sidewall was observed to increase at higher plug speed and vacuum delay time of 0 ~ 0.3sec.

• 소성∙가공
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• 제22권3호
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• pp.171-177
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• 2013

Vacuum-assisted thermoforming is one of the critical steps for successful application of film insert molding (FIM) to make parts of complex shape. If the thickness distribution of the formed film is non-uniform, then cracking, deformation, warpage, and wrinkling can easily occur at the injection molding stage. In this study, the simulation of thermoforming was performed to predict the film thickness distribution, and the results were compared with experiments. Uniaxial tensile tests with a constant crosshead speed for various high temperatures were conducted to investigate the stress-strain behavior. An instance of yielding occurred at the film temperature of $90^{\circ}C$, and the film stiffness increased with increasing crosshead speed. Two types of viscoelastic models, G'Sell model, K-BKZ model, were used to describe the measured stress-strain relationship. The predicted film thickness distributions were in good agreement with the experimental results.

• 소성∙가공
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• 제21권1호
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• pp.30-35
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• 2012

FIM(Film Insert Molding) is an innovative method of producing decorated parts for a wide range of products. Because it requires fewer steps when compared to conventional production methods, the time and cost of manufacturing high quality components can be reduced considerably. In this paper, the effects of processing conditions on the warpage of film-insert molded plate were investigated by using a design of experiments. The dominant factors affecting warpage were mold temperature and holding pressure. Warpage increased with the temperature difference between stationary mold and fixed one. Even when the mold temperature difference was zero, the plate with a film was bent after ejection such that the film side protruded. As holding pressure increased, warpage decreased significantly. In addition warpage increased with time increment for the film-insert molded plates.

• 대한수의학회지
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• 제63권2호
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• pp.16.1-16.6
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• 2023

Salmonella spp. are pathogens involved in most salmonellosis in rabbits. This study examined Salmonella disease in rabbits raised in Thua Thien Hue, Vietnam. Two hundred and 56 rectal swabs of rabbits were taken, and a carrier rate of 33.98% was found. In addition, all the isolated Salmonella spp. strains were 100% motile; positive for H₂S, catalase, Voges Proskauer, coagulase, citrate, maltose, and dextrose; and negative for indole, methyl red, urease, oxidase, sucrose, and lactose. The Kirby-Bauer method showed that these Salmonella strains were susceptible to doxycycline (93.2%), tetracycline (84.1%), and levofloxacin (65.9%). On the other hand, they were highly resistant to streptomycin (95.5%), ampicillin (93.2%), colistin (40.9%), and gentamicin (34.1%). Furthermore, polymerase chain reaction used to screen for virulence and specific genes of Salmonella strains showed that all Salmonella strains isolated carried InvA, fimA, and Stn.

• Smart Structures and Systems
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• 제13권3호
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• pp.389-406
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• 2014

Optimal sensor placement techniques play a significant role in enhancing the quality of modal data during the vibration based health monitoring of civil structures, where many degrees of freedom are available despite a limited number of sensors. The literature has shown a shift in the trends for solving such problems, from expansion or elimination approach to the employment of heuristic algorithms. Although these heuristic algorithms are capable of providing a global optimal solution, their greatest drawback is the requirement of high computational effort. Because a highly efficient optimisation method is crucial for better accuracy and wider use, this paper presents an improved simulated annealing (SA) algorithm to solve the sensor placement problem. The algorithm is developed based on the sensor locations' coordinate system to allow for the searching in additional dimensions and to increase SA's random search performance while minimising the computation efforts. The proposed method is tested on a numerical slab model that consists of two hundred sensor location candidates using three types of objective functions; the determinant of the Fisher information matrix (FIM), modal assurance criterion (MAC), and mean square error (MSE) of mode shapes. Detailed study on the effects of the sensor numbers and cooling factors on the performance of the algorithm are also investigated. The results indicate that the proposed method outperforms conventional SA and Genetic Algorithm (GA) in the search for optimal sensor placement.

• Biomolecules & Therapeutics
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• 제13권2호
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• pp.101-106
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• 2005

The generation of secretory IgA antibodies (Abs) for specific immune protection of mucosal surfaces depends on stimulation of the mucosal immune system, but this is not effectively achieved by parenteral or even oral administration of most soluble antigens. Thus, to produce a possible vaccine antigen against urinary tract infections, the uropathogenic E. coli (UPEC) adhesin was genetically coupled to the heat-labile Escherichia coli enterotoxin A2B (ltxa2b) gene and cloned into a pMAL-p2E expression vector. The chimeric construction of pMALfimH/ltxa2b was then transformed into E. coli K-12 TB1 and its nucleotide sequence was verified. The chimeric protein was then purified by applying the affinity chromatography. The purified chimeric protein was confirmed by SDS-PAGE and westem blotting using antibodies to the maltose binding protein (MBP) or the heat labile E. coli subunit B (LTXB), plus the N-terminal amino acid sequence was analyzedd. The orderly-assembled chimeric protein was confirmed by a modified $G_{M1}$-ganglioside ELISA using antibodies to adhesin. The results indicate that the purified chimeric protein was an Adhesin/LTXA2B protein containing UPEC adhesin and the $G_{M1}$-ganglioside binding activity of LTXB. thisstudy also demonstrate that peroral administration of this chimeric immunogen in mice elicited high level of secretory IgA (sIgA) and serum IgG Abs to the UPEC adhesin. The results suggest that the genetically linked LTXA2B acts as a useful mucosal adjuvant, and that adhesin/LTXA2A chimeric protein might be a potential antigen for oral immunization against UPEC.

• Journal of Astronomy and Space Sciences
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• 제18권3호
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• pp.239-248
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• 2001

2002년에 발사 예정인 과학 위성 1호의 주 탑재체로 원자외선 분광기가 실릴 예정이다. 원자외선 분광기는 영상과 분광의 기능을 함께 수행하게 되는데, 광학 부품 중 포물 원통 만가경의 경우 천문 관측용으로는 제작된 바가 없어 제작과 이에 관련된 측정 방법을 새로이 고안하여 제작시험이 이루어 겼다. 관측 목표로부터 도출된 사양 중에서 성능과 가장 밀접한 관계가 있는 형상오차의 경우 ~ $lambda/cm$의 한계를 만족하였으며, 산란장과 연관된 표면 거칠기의 경우 새로이 고안된 비구면 pitch polishing의 방법으로 $R_{q}$의 값이 1 nm 이하로 감소하였으며, 이로써 전체 파장 영역에서 0.02, 즉 2% 미만의 빛이 산란됨을 간접적으로 확인하였다.

• 전기화학회지
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• 제8권4호
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• pp.181-185
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• 2005

알루미나 기판을 사용하여 백금 박막 전류 집전체상에 $2.9{\mu}m$ 두께 및 $4cm^2$의 전극면적을 갖는 $LiCoO_2$ 박막을 R.F. 마그네트론 스퍼터링법에 의해 증착하였으며, 아르곤 공정 압력 및 인가된 R.F. 전력량에 따른 Li/Co 몰 비 의존성에 대해 고찰하였다. 비정질계 고체전해질인 Lipon 및 Li 음극이 순차적으로 증착된 박막전지를 제조하여 정전류충, 방전 시험하였으며, 고율방전 특성 및 충, 방전 횟수에 따른 전지 용량 변화를 측정하였다. 교류임피던스를 통해 전지내부의 저항성분을 측정하였으며, 이에 대한 등가회로를 구성하여 시뮬레이션한 결과와 비교하였다.

• Biomolecules & Therapeutics
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• 제11권3호
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• pp.157-162
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• 2003

The generation of secretory IgA antibodies(Abs) for specific immune protection of mucosal surfaces depends on stimulation of the mucosal immune system, but this is not effectively achieved by parenteral or even oral administration of most soluble antigens. Thus, to produce a possible vaccine antigen against urinary tract infections, the uropathogenic E. coli (UPEC) adhesin was genetically coupled to the ctxa2b gene and cloned into a pMAL-p2E expression vector. The chimeric construction of pMALfimHIctxa2b was then transformed into E. coli K-12 TB1 and its nucleotide sequence was verified. The chimeric protein was then purified by applying the affinity chromatography. The purified chimeric protein was confirmed by SDS-PAGE and western blotting using antibodies to the maltose binding protein (MBP) or the cholera toxin subunit B (CTXB), plus the N-terminal amino acid sequence was analyzed. The orderly-assembled chimeric protein was confirmed by a modified $G_{M1}$-ganglioside ELISA using antibodies to adhesin. The results indicate that the purified chimeric protein was an Adhesin/CTXA2B protein containing UPEC adhesin and the $G_{M1}$-ganglioside binding activity of CTXB. This study also demonstrate that peroral administration of this chimeric immunogen in mice elicited high level of secretory IgA and serum IgG Abs to the UPEC adhesin. The results suggest that the genetically linked CTXA2B acts as a useful mucosal adjuvant, and that the adhesin/CTXA2B chimeric protein might be a potential antigen for oral immunization against UPEC.

• Clinical and Experimental Pediatrics
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• 제55권11호
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• pp.420-423
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• 2012

Purpose: We phylogenetically analyzed the Escherichia coli strains isolated from children with urinary tract infection (UTI) in 2 regions of Korea. Virulence factors (VFs) and antibiotic resistance of the strains were also determined to compare the possible differences. Methods: A total of 138 E. coli strains were collected from the 2 regions; Gyeongin (78 strains) and Gyeongnam (60 strains). The phylogenetic groups were determined using the triplex polymerase chain reaction (PCR) method and multiplex PCRs were used to detect 7 VFs genes (fimH, papC, iutA, hlyA, sfa/focDE, afa/draBC, and kpsMT II). We also tested for antibiotic resistance. Results: Phylogenetic groups, B2 (61.6%) and D (26.8%), comprised the majority of all isolated strains. Regional comparisons revealed that more B2 strains and fewer non-B2 (A+B1+D) strains were found in Gyeongnam, than in the Gyeongin region (P=0.033), and certain VFs were predominantly detected in Gyeongnam (P<0.05). Neither regional nor phylogenetic differences, in antibiotic resistance of the strains, were significant. Conclusion: We were able to confirm that the geographic location is an important determinant of the distribution of the phylogenetic groups and VFs among the E. coli strains that cause UTI in children.