• Korean Journal of Environmental Biology
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• v.37 no.1
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• pp.60-67
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• 2019

The objective of this study was to verify the stability of nutrient composition by using herbal medicine by-products as an alternative food source and to examine the growth effect on Protaeria brevitarsis seulensis larvae. As a result of comparing the nutritional components of food source, crude protein, crude fat, and crude ash content, except crude fiber content, was high in both non-fermented and fermented medicinal herbal by-products. Especially, crude protein content was highest. Cadmium, lead, mercury, and other heavy metals were not detected and thus stability as alternative food was confirmed. The growth comparison based on the feeding sources showed no significant difference between the fermented oak sawdust fed control group and the herbal medicine by-products fed laboratory group from week 1 to week 3. The weight of a 4 week larva was 0.137 g in the control group and 0.671 g in the laboratory group and so began to reveal differences at a significant level (p<0.05). As a result of comparing weights of Protaetia brevitarsis seulensis larvae according to the level of herbal medicine by-product addition, HMB40 recorded the heaviest weight in week 7. Statistical analysis showed that there was no significant difference in each body weights of HMB40 and HMB80 at week 5 (p<0.05). These results indicate that if the shipping date of an edible insect is a third instar larva, it arrives at the time of shipment at week 5. Thus feeding HMB40 and HMB80 at the 5th week is the most effective.

• Journal of Sericultural and Entomological Science
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• v.50 no.1
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• pp.27-32
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• 2012

The aim of this study is to develop indoor-rearing methods of the diving beetles. In nature, both the adult diving beetle and its larvae are voracious aquatic predators. The larvae beetles hunt relatively bigger size of tadpoles and small fish for food source. However, due to difficulties of the food supplement for rearing diving beetles at indoor-condition further motivated us to develop new artificial food. Three separate experiments were performed. In the first experiment, adult beetles were provided with one of the several food choice treatments to self-compose their preferred foods that are affordable on the market at lower price. The second experiment was also to develop artificial diet that is possible for rearing larvae beetle under indoor condition. The larvae beetles were restricted to raw squid, artificial food source and mosquito larvae as a control at the first stadium and small fish and raw squid during second to third stadium duration. According to our result, adult beetles selected a food that made of boiled squid and dead small fish while, the young larvae consumed small fish, mosquito larvae and raw squid. Although, the larval food restriction on law squid caused noticeable decrease in survival, the result still supported the possible survival rate of keeping larvae at indoor condition. Moreover, pupation rate experiments, in which groups of larvae were placed at different mats, natural soil and fermented sawdust, showed that 80% of diving beetles pupated on the sawdust. This result indicates that female beetle preferentially selected to oviposit along soft and moist area.

• Journal of Life Science
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• v.27 no.6
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• pp.702-707
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• 2017

Insects and their extract have been traditionally used as medicinal resources in Asia. Recently, Gryllus bimaculatus and the larva of Tenebrio molitor, Protaetia brevitarsis, and Allomyrina dichotoma were enlisted as general food ingredients by Korean Ministry of Food and Drug Safety. As the interest in these insects is growing, the detailed investigation is needed for the standardization of suitable and safe rearing techniques. This survey was conducted via personal interviews about edible insect rearing farms in Korea. The basic feed is fermented oak sawdust for P. brevitarsis and A. dichotoma and wheat bran for T. molitor. The various feed additives are blended with each farm's own recipes. The overall growth condition, including sawdust fermentation method, feeding period, and rearing density, varies among the investigated farms. It seems that weight of last instar larva was correlated with the rearing density and duration of the larval period. The heavy metal contents were analyzed after 48 hour starvation and they were very small amount or not detected in investigated insects. We believe that the present data will contribute to develop and standardize the safe rearing techniques of edible insects in Korea.

• Journal of the Korea Organic Resources Recycling Association
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• v.2 no.1
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• pp.51-64
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• 1994

To compost the food garbage with the dry bean curd and sawdust as the bulking agents, the method of high-speed fermentation by the characteristic microorganisms group was applied. The results of experiments are summarized as follows ; 1. Korean food garbage, which is high in water content, is difficult to compost only by microorganism fermentation without the addition of bulking agents such as dry bean curd cake and sawdust. 2. Weight reduction rates are ranging from 35.6% to 64.5% and varying with the composition of food garbage. The less weight reduction rate is, the longer continuous-fermentation is. And the color of compost is changing sequentially as yellow -> brown -> black. 3. Comparing with the controlled microorganism group, the weight reduction rate and $H_2CO_3$ production rate in the characteristic microorganism group fermentation reactors are higher. And the fermentation rate is satisfactory when the characteristic microorganism group is added. 4. The value of fermented composting as fertilizer diminishes, and the contents of Total Nitrogen, $P_2O_5$, $K_2O$ increase on the condition that the fermentation continues. However, the organic contents and C/N ratio diminish as the fermentation continues. 5. The high-speed fermentation technology demonstractes the possibility of recycling as well as the reduction of composting time provided that it is applied as a pretreatment process for composting.

• Journal of The Korean Society of Grassland and Forage Science
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• v.20 no.2
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• pp.139-146
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• 2000

A trial was carried out to investigate the effect of fermented saw-dust pig manure (FSP) and N fertilizer application on physical, chemical properties and microbial population of soil on Cheju brown volcanic ash pasture during the period from September, 1997 to January, 1999. Average soil N contents during 3 different periods, August and October, 1998, and January, 1999 were 0.39, 0.41 and 0.39% for fertilizer N level 0, 150 and 300kg/ha, respectively. Soil N contents determined in January, 1999, was significantly increased by an increase of fertilizer N. Nitrogen contents in the soil applied with 0, 3, 6 and 12 tons 1 ha of FSP were 0.43, 0.40, 0.38 and 0.38%, respectively, showing decreasing tendency of soil with increasing levels of FSP application. Soil N contents determined in August, 1998 and January, 1999, were significantly decreased by increasing levels of FSP application. Organic matter contents of the soil applied with N 0, 150 and 300 kg/ha was 8.04, 8.37, and 9.08%. Soil organic matters determined on the 1st and 2nd period trended to increase with increasing level of fertilizer N. FSP application significantly decreased organic matter contents of pasture soil, (9.14, 8.79, 8.28 and 7.78% OM in soil applied with FSP 0, 3, 6 and 12 tonlha of FSP), respectively. Soil OM determined in October, 1998, also showed a significant decrease with increasing level of FSP. Soil pH, available phosphorus, exchangeable K, Ca, Mg and Na in soil studied were not influenced by fertilizer N or FSP application during all three periods. N application tended to increase soil bacteria count, ($27.0\;{\times}\;10^4\;cfu/g,\;29.4\;{\times}\;1-^4\;cfu/g$ and $53.6\;10^4\;cfu/g$ in the soil applied with 0, 150 and 300 kg N/ha, respectively). The number of colonies of soil bacteria and fungi determined in June and October, 1998 was not increased remarkably by FSP application, but the number of colonies of bacteria determined in March, 1998 showed a significant increase with increasing level of FSP application. In conclusion, N contents and OM of soil increased with increasing level of N application, but decreased with increasing level of FSP application.

• Membrane Journal
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• v.20 no.2
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• pp.127-134
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• 2010

This study is conducted about the system that reduces organism after fermenting food waste from a food waste disposal equipment, divides gas made when food waste is fermented into gas and water, and then sends gas to a reactor again, condenses water, and apply it to the MBR system with submerged MF hollow fiber membranes. A submerged MF hollow fiber membrane module was installed to a food waste disposal equipment and a water treatment system made by Bio Hitech Co,. Ltd. to process food waste generated from a staff cafeteria in a H institute for 90 days. For initial seeding of a food waste disposal equipment, 305 kg of rice bran, chaff, and sawdust as well as 1,648 kg of food were input during the operation, and 1,600 L of condensed wastewater occurred. Fermented by-product after finishing running a food waste disposal equipment was 386 kg and its reduction was shown to be 80%. The organism was processed by applying submerged MF hollow fiber membrane module to the MBR system of condensed wastewater, and the result shows reduction rates were BOD 99.9%, COD 97.5%, SS 98.6%, T-N 54.6% and T-P 34.7% and the total colon bacillus was perfectly eliminated.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.4
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• pp.245-252
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• 1995

To select a carrier as Rhizobiwn inoculants for alfalfa, severeal Rhizobium inoculants were produced by adding or not sub-materials($CaCO_3$ and sucrose) to main materials(organic or inorganic materials). The root nodule bacteria, Rhizobium meliloti 1061 distributed from KAIST, and Vemal alfalfa was used in this experiment. The Rhizobium populations and inoculation effects of the Rhizobium inoculants produced in several materials were scrutinized at laboratory and greenhouse in Livestock Experiment Station, RDA from 1993 to 1994. Moisture contents of the caniers were varied from 32 to 50% on dry weight basis according to material characteristics and the pH ranges of these were varied from pH 4.56 to 10.06 according to raw material characteristics and preparations. Initial Rhizobium numbers of the carriers were higher in organic material-inoculants than in inorganic, and among the inoculants, the inoculant made of Bentonite+Vermiculite(l:3 w/w) was excellent because of high rhizobium population($7.8~8.3\times10^8/g$ inoculant) and high rhizobium reappearance of inoculant in severed different production time. The root nodules of the alfalfa inoculated with different inoculants were fast formed in the fermented sawdust with cattle dung (FSC) inoculant, and bentonite(B)+vermiculite(V) than others. Plant length of alfalfa was differentiated on 15 days after inoculation but was not nearly different between higher inoculants than rhizobium number $10^7/g$ inoculant. Total dry matter of alfalfa was yielded by 20.65, 20.34mg per pot in FSC + sucrose 0.5% and B + V + sucrose 1% inoculants respectively that were higher inoculation effect by 17 times compared with non-inoculation, 1.2 mg per pot.

• International Journal of Industrial Entomology and Biomaterials
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• v.26 no.1
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• pp.61-66
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• 2013

Baculum elongatum has several peculiarities such as parthenogenesis, unique external features, changing body color, and dropping oviposition, which makes it a potential economically useful insect. Oviposition was performed by females without fertilization by the sperm of males. The oviposition period was $42.2{\pm}22.7$ days and the number of eggs per female was $109.5{\pm}70.5$ eggs. The hatch rate was 73.3% at $25^{\circ}C$ and 66.7% after low temperature treatment ($8^{\circ}C$ for 60 days). In nature, B. elongatum overwinters as an egg, but it can be assumed from the results that cold temperatures were not required for hatching. The hatch rate was 98.2% in the treatment using floral foam, fermented sawdust, and leaves. The developmental period was $100.9{\pm}4.2$ days for eggs, $55.3{\pm}4.6$ days for nymphs, and $49.7{\pm}16.0$ days for adults. The length of the eggs was $0.33{\pm}0.0$ cm and the lengths of the nymphs were clearly distinguishable according to the instar stage. Clover(Trifolium repens) was an excellent diet as it was similar to the host plant and could be used as an alternative diet. The rate of reaching adulthood for the insects was 66.7% on an artificial diet containing 25% acacia leaves. For sustainable mass rearing of nymphs or adults of B. elongatum, a natural diet could be used such as acacia (Robinia pseudoacacia), white oak (Quercus aliena), chestnut (Castanea crenata var. dulcis), and bush clover (Lespedeza bicolor), or an alternative diet such as clover or artificial diet.

• Food Science of Animal Resources
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• v.40 no.3
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• pp.461-473
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• 2020

The goal of this study was to evaluate the influence of various supplementary feeds on the chemical composition and production of bioactive substances in Protaetia brevitarsis larvae. The primary feed-oak-fermented sawdust-was supplemented with a variety of substances, including aloe, apple, banana, sweet persimmon (S. persimmon) and sweet pumpkin (S. pumpkin). Crude protein and fat content were the highest in the control and S. pumpkin group, respectively. Supplementary feeds increased the content of unsaturated fatty acids, except in the group receiving S. pumpkin, in which oleic acid was the most abundant (58.2%-64.5%). Free essential amino acids in larvae receiving supplementary aloe were higher compared with the control group except for Lys and His. Polyphenol and flavonoid contents and the antioxidant activities of ABTS and DPPH were higher in all treated groups compared with the control group. Although supplementary feeds led to a decreased crude protein content in the treated larvae when compared with the control group, these treatments generally improved the levels of unsaturated fatty acids and antioxidative activity. Therefore, we suggest that among the supplementary foods tested, aloe is a better resource for P. brevitarsis based on crude protein content, free amino acids and other bioactive compounds such as unsaturated fatty acids and antioxidants.

• Journal of Mushroom
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• v.10 no.4
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• pp.236-243
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• 2012

Spent mushroom substrates is composted organic material remaining after a crop of mushroom is harvested. The raw materials of mushroom substrates are same feed ingredient as corncobs, rice brown, wheat brown, cotton seeds and beet pulp. During the mushroom cultivation process, the mushroom substrates was used 15-25% by mushroom and 75-85% of mushroom substrates was remained in the SMS. Among of the spent mushroom substrates, spent mushroom substrates of pleurotus eryngii, Pleurotus ostreatus and Flammuliua velutipes is can be use the energy feedstock of animal feed. The cellulose content of spent mushroom(pleurotus eryngii) substrates containing the sawdust was high and total digestible nutrients (TDN) values was low. The spent mushroom(pleurotus eryngii) substrates fermented with cellulase and xylanase producing bacteria is may be used as an ingredient of feed in TMR for Hanwoo steer.