• 제목/요약/키워드: Fermented Rice Extract

검색결과 55건 처리시간 0.023초

현미 발효 슬러리의 항균활성 (Antimicrobial Efficiency in the Fermented Slurry of Unpolished Rice)

  • 최학준;곽경자;최다빈;박재영;정현숙
    • 한국미생물·생명공학회지
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    • 제43권4호
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    • pp.307-313
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    • 2015
  • 현미는 도정한 백미보다 이로운 영양분을 더 많이 함유하고 있다. 본 연구에서는 현미를 이용하여 만든 현미 발효 슬러리의 이화학적 특성과 항균활성에 대해 시험하였으며, 현미발효슬러리의 항균활성은 paper disc-agar diffusion 방법을 이용하여 6가지 병원성 균주(Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella typhimurium and Yersinia enterocolitica)와 2가지 발효균주(Gluconacetobacter intermedius and Lodderomyces elongisporus)에 대해 항균성을 조사하였다. 특히, Staphylococcus aureus, E. coli, Listeria monocytogenes, P. aeruginosa, Salmonella typhimurium, Y. enterocolitica 그리고 Lodderomyces elongisporus에 대해서는 시판 항생제인 카베니실린과 테트라사이클린보다 더 높은 항균활성을 보였다. 항산화활성은 2,2-diphenyl-1-picrylhydrazyl (DPPH) 라디칼 소거능을 이용하여 측정하였을 때, 대표되는 항산화제인 아스코르빅 산과 비슷한 활성을 나타내었다. 현미발효슬러리의 발효중에 나타나는 균주를 동정하기 위해 TSB 고체배지와 YPD 고체배지에 현미발효슬러리를 도포하였을 때, 분리된 콜로니를 16S rDNA sequence 분석을 통하여, 네가지 균주를 분리하였으며, phylogenic tree 분석법을 이용하여 조사하였을 때, 각각 G. intermedius, Lactobacillus casei, Lactobacillus plantarum 그리고 Acetobacter peroxydans와 유사하였다.

Inhibitory Effect of Korean Fermented Soybean (Chungkookjang) Extract and Genistein Against Trp-P-1 Induced Genotoxicity in HepG2 Cells

  • Song, Eun Jeong;Kim, Nam Yee;Heo, Moon Young
    • 한국식품위생안전성학회지
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    • 제32권3호
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    • pp.171-178
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    • 2017
  • 청국장추출물과 청국장의 주요한 플라보노이드의 하나인 genistein의 HepG2 세포에서 Trp-P-1 유도 세포독성과 DNA손상에 대한 보호효과를 평가하였다. 청국장추출물과 주요 플라보노이드성분 genistein은 Trp-P-1 유도 세포독성에 대하여 세포독성보호효과를 나타내었다. 청국장추출물은 Trp-P-1 유도 DNA single strand breaks를 억제하였다. 한편, 청국장추출물은 HepG2 세포에서 Trp-P-1 유도에 의한 CYP1A1와 CYP1A2 발현의 억제를 나타내었다. 청국장추출물과 genistein은 Trp-P-1에 의한 유도 세포독성과 DNA손상에 대하여 CYP1A1, CYP1A2 발현억제에 의하여 보호효과가 나타나는 것으로 판단된다. 한국의 전통 콩발효식품인 청국장은 게놈 불안정성(genomic instability)을 일으키는 heterocyclic amines (HCAs)과 같은 식품의 가열조리로부터 올 수 있는 발암물질에 대한 유전독성을 예방할 수 있는 유망한 기능성물질로서 활용가능성이 있을 것으로 판단된다.

Effects of Rice Bran Extracts Fermented with Lactobacillus plantarum on Neuroprotection and Cognitive Improvement in a Rat Model of Ischemic Brain Injury

  • Hong, Jeong Hwa;Kim, Ji Yeong;Baek, Seung Eun;Ingkasupart, Pajaree;Park, Hwa Jin;Kang, Sung Goo
    • 대한의생명과학회지
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    • 제21권2호
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    • pp.92-102
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    • 2015
  • This work aimed to study whether rice bran extract fermented with Lactobacillus plantarum (LW) promotes functional recovery and reduces cognitive impairment after ischemic brain injury. Ischemic brain injury was induced by middle cerebral artery occlusion (MCAO) in rats. Four groups were studied, namely the (1) sham, (2) vehicle, (3) donepezil, and (4) LW groups. Animals were injected with LW once a day for 7 days after middle cerebral artery occlusion. LW group showed significantly improved neurological function as compared to the vehicle group, as well as enhanced learning and memory in the Morris water maze. The LW group showed the greatest functional recovery. Moreover, the LW group showed an enhanced more survival cells anti-apoptotic effect in the cortex and neural cell densities in the hippocampal DG and CA1. In addition, this group showed enhanced expression of neurotrophic factors, antioxidant genes, and the acetylcholine receptor gene, as well as synaptophysin (SYP), Fox-3 (NeuN), doublecortin (DCX), and choline acetyltransferase (ChAT) proteins. Our findings indicate that LW treatment showed the largest effects in functional recovery and cognitive improvement after ischemic brain injury through stimulation of the acetylcholine receptor, antioxidant genes, neurotrophic factors, and expression of NeuN, SYP, DCX, and ChAT.

Lactobacillus sakei B2-16에 의한 γ-amino butyric acid(GABA)의 생산에 관한 연구 (Study of γ-Amino Butyric Acid (GABA) Production by Lactobacillus sakei B2-16)

  • 국무창;조석철;최찬익;박훈;김승섭;정명훈;변유량;이현용
    • 산업식품공학
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    • 제13권3호
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    • pp.183-189
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    • 2009
  • 혈압저하작용, 이뇨기능 등의 다양한 생리활성을 나타내는 $\gamma$-amino butyric acid(GABA)를 고농도로 생산하는 유산균인 Lactobacillus sakei B2-16을 이용하여 GABA의 산업적 생산 배지를 연구하였다. L. sakei B2-16의 최적 상용 배지는 Lactobacilli MRS 배지였으며, Lactobacilli MRS 배지에 1% mono sodium glutamate(MSG)를 첨가하고, L. sakei B2-16을 배양했을때 MSG의 99.3% 는 GABA로 전환되었다. MRS 배지를 기본으로 최적 배지조성을 검토한 결과, 탄소원으로 4% sucrose와 질소원으로 1% yeast extract를 첨가하였을 때 균체 증식과 GABA 생산량이 가장 우수하였다. 산업적 배지를 확립하기 위하여 미배아를 온수 추출하여 얻은 추출액 배지에 L. sakei B2-16을 배양한 결과, 7%의 MSG를 100% GABA로 전환시켰으며, 미배아 추출액을 이용한 배지는 산업적 생산용 배지로의 응용이 기대된다.

TLC법에 의한 장류 및 공류중의 Aflatoxin검출에 관한 연구 (The Study on the Detection of Aflatoxins in the Fermentation Products and Cereals)

  • 한양일;김광호;오영복
    • 한국환경보건학회지
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    • 제5권1호
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    • pp.46-50
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    • 1978
  • Aflatoxin, a mixture of the at least four toxic and carcinogenic metabolites, is known to be produced by only a few fungi. The toxins were designated aflatoxins because they were produced by the mold Aspergillus flavus(A. flavus). However, at least four other toxins and other species of the genus A. niger, A. parasiticus A. ruber and wentii have been reported to produce aflatoxins. And also the identical compounds may also be produced by molds, the Pencillium. At least four different species of Penicilliurn have been reported to produce aflatoxins (P. citrinurn, P. frequentans, P. puberulurn. and P. variable). So it is now known that the problem of Aflatoxin is not restricted to the single species A. flavus, even though that is a very common mold. Also additional aflatoxins have been discorvered. For sereral years, only four aflatoxins were known: $B_1, B_2, G_1$ and $G_2$, so designated by reason of their fluorescence and chromatographic charateristics. It is now known that there are really two new toxic materials in the milk. During the past year(1966) they were christened aflatoxin $M_1$ and $M_2$, since they were first found in milk. The two other and most recently discorvered aflatoxins were isolated late in 1966 from cultures of A. flavus, and were designated aflatoxin $B_2a$ and aflatoxin $G_2a$. In order to obtain a breaf information about extent of contamination of foodstuffs by aflatoxin which is known to produce eight different mold, aflatoxin detection of cereals and fermented foods on sale, such as polished rice, barley, wheat, wheat flour, lentil, red bean, soy bean, noodle, kochuj ang and Dwenjang (fermented soy bean paste) and chong Kuk, were carried out. The results of this investigation were summarized as follows: The hexane:$CHCl_3$ extracts of polished rice, barley wheat, wheat flour, lentil, red bean, noodle and kochujang yielded fluorescent spots on thin layer plates. However their Rfvalues were different from those of authentic aflatoxins. The fluorescent substances of the extract from soy bean, Dwenjang and chong kuk showed very similar Rf values to those of the standard aflatoxins. By two dimensional thin layer chromatography and comparison of ultra violet absorption spectra, it was found that these fluorescent substances were not aflatoxins. To conclude, aflatoxins themselves were not detected directly in those samples tested.

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유산균을 이용한 발효약초부각의 영양성분, 조직감 및 항산화 (Nutritional Components, Texture, and Antioxidant Properties of Lactic Acid Bacteria-Fermented Yakchobugak with Addition of Agro-food Products)

  • 고영란;손미예;왕수빈;이광수;강성구;박석규
    • 한국식품저장유통학회지
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    • 제16권3호
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    • pp.405-411
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    • 2009
  • 기호성과 품질성이 우수한 전통부각을 개발하기 위하여 천연색소 함유 농산물과 유산균을 이용한 발효약초부각의 공정개발과 그 품질특성을 조사하였다. 약초부각의 탈유율은 정치 3.5%나 타봉 8.9%에 비하여 원심력이 약 37.6%로서 효과적이었다(p<0.05). 환원력은 부재료 추출물의 농도에 비례적으로 증가하였으며, 녹차는 다른 추출물에 비하여 유의적 차이가 크게 나타났으며, $500{\mu}g/mL$에서 0.97로 가장 높게 나타났다(p<0.05). DPPH 자유라디칼 소거능은 $50{\mu}g/mL$에서 녹차가 83.84%로 가장 높았으며, 다음으로 울금 39.48%, 흑미 28.45%와 복분자 22.25% 순이었다(p<0.05). 산가는 $60^{\circ}C$로 7일간 고온저장한 녹차와 복분자 발효약초부각이 각각 1.82, 2.03%였으며, 14일까지는 울금과 복분자 발효약초부각이 7.24, 7.67%로 효과적이었다(p<0.05). Hardness값은 흑미와 울금을 제외한 발효약초부각은 250.62 이하로 아주 낮았으며, 유산균발효 공정은 $2{\sim}3$배의 조직감 개선에 효과적이었다. 발효약초부각의 탄수화물과 단백질은 $56.8{\sim}64.6%$, $6.8{\sim}$8.7%이었고, 지방은 울금과 녹차 발효약초부각이 각각 22.1, 24.8%로 비교적 낮은 함량이었으며, 열량은 울금이 487 Kcal로 가장 낮았다.

소곡주의 양조과정중 술덧성분과 미생물의 변화 (Chanties in the Chemical Components and Microorganisms in Sogokju-Mash during Brewing)

  • 소명환
    • 한국식품영양학회지
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    • 제5권2호
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    • pp.69-76
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    • 1992
  • Sogokju, an old Korean rice wine, was brewed in laboratory scale in February, 1978, and the changes in chemical component and microorganisms in wine mash were investigated during brewing. In the course of first-mash brewing, lactic acid bacteria became the most predominant microflora in 3 days of fermentation lowering the pH of mash to 4.2. But at 7 days, the most predominant microorganism was changed from lactic acid bacteria to least, pH reached 3.6, and the saccharogenic activity of mash was reduced from initial 10 to 3.6. During second-mash brewing, remarkable changes did not occurred in pH and acidity, maintaining 3.5∼3.7 of pH and 8.0∼8.8 of acidity. Ethanol fermentation was proceeded vigorously until 20 days of fermentation, but it reached stationary state with 18.5% ethanol after 40 days, leaving reducing sugar unfermented in mash. Even if saccharogenic activity continued all during fermentation, it was thought to be too weak. The final product of Sogokju filterate, fermented for 60 day, contained 18.8% ethanol, 5.0% reducing sugar,0.4:1 total acid, 0.057% volatile acid, 0.206% amino acid and 7.3% extract. In sensory test, Sogokju scored good marks with similar level to commercial Bupju or Chungju. It had pale yellow color, gentle Nuluk-flavor and acid taste just a little strong.

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소국주(小麴酒)와 시판약주(市販藥酒)의 성분(成分)에 관(關)한 연구(硏究) (Studies on the Components of Sokokju, and Commercial Yakju)

  • 장기중;유태종
    • 한국식품과학회지
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    • 제13권4호
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    • pp.307-313
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    • 1981
  • 한국(韓國) 고래주(古來酒)인 소국주(小麴酒)와 시판약주(市販藥酒)의 성분(成分)이 품질(品質)에 미치는 영향을 연구한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 1차 담금에 찹쌀 반숙미(半熟米)를 사용(使用)하여 양조(釀造)한 소국주(小麴酒)에 있어서 알코올과 추출물(抽出物)의 함유량(含有量)이 높았으며 다량(多量)의 pyruvic acid가 검출(檢出)되 었다. 2. 타르타르산과 시트르산은 시판(市販) 약주(藥酒)에서만 검출(檢出)되었으며 소국주(小麴酒)의 주된 유기산(有機酸)은 락트산$(7{\sim}10\;mg/ml)$ 및 푸마르신과 숙신산$(0.3{\sim}0.65\;mg/ml)$이였다. 3. 소국주용(小麴酒用) 누룩에는 락트산이 7.8 mg/g 함유(含有)되어 있었으며 시판(市販) 약주용(藥酒用) 누룩에서는 말산이 0.47 mg/g으로 다량(多量) 함유(含有)되어 있었다. 4. 관능(官能) 검사(檢査) 결과(結果) 참쌀 반숙미(半熟米)를 사용(使用)하여 양조(釀造)한 소국주(小麴酒)가 다른 약주(藥酒)보다 품질(品質)이 우수하였다.

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황칠·미강 발효 추출물이 tyrosinase 활성 저해 및 항산화에 미치는 효과 (Effects of Vinegar Fermentation of Korean Dendorpanax and Rice Bran Mixture on the Activity of Tyrosinase and Anti-Oxidant in B16F10 Cell Line)

  • 최찬헌;안정은;임성수;정현우
    • 동의생리병리학회지
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    • 제29권3호
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    • pp.273-280
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    • 2015
  • In this study, we used the mixture made from the Rice bran 45 ㎏, Dendropanax 5 ㎏, the sugar of the 10% of the total weight, and the enzyme of the 0.1% of the total weight. After the mixture were fermented for 90 days under 20 $^{\circ}C$, we measured the cell viability and the inhibition rate of the melanin biosynthesis, the activity of tyrosinase and superoxide dismutase (SOD) in malignant melanoma, B16F10 cells, in order to survey the whitening effect and the mechanism of the effects on the sample. As a result, the samples significantly suppressed the cell viability of B16F10 in more than 500 ${{\mu}g}$/㎖ and significantly inhibited the generation of melanin induced by ${\alpha}$-MSH in more than 1,000 ${{\mu}g}$/㎖. Sample decreased the activity of tyrosinase while increased the activity of SOD in dose dependent manner. Therefore, we considered that the fermentation extract made from a Rice bran and Dendropanax will be able to produce high value-added products, if used as a commercial.

청갓과 청갓김치의 핵산관련물질의 동정 및 함량에 관한 연구 (Studies on Identification and Composition of Nucleosides from Mustard Leaf and Mustard Leaf Kimchi)

  • 김재이;최재수;김우성;최홍식
    • 한국식품영양과학회지
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    • 제29권5호
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    • pp.796-801
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    • 2000
  • Kimchi is the Korean traditional food which is fermented properly from salted Korean cabbage of raddish with other various supplements. Kimchi therefore can be the major sources for various kinds of nutrients and other biological substances. The fermentation process accompanies with complicated reaction mechanism which bacteria, fungi and yeast are involved and they produced aroma, taste and bioactive components. To identify nucleoside, this study was conducted with freeze-dried mustard leaf, mustard leaf kimchi and fermented mustard leaf kimchi. Hexane, CH$_2$Cl$_2$, EtOAc and BuOH was used in order to extract their components. The isolated compounds I and II from mustard leaf and mustard leaf kimchi were identified as adenosine and uracil using UV, $^{1}H$-NMR, $^{13}C$-NMR and LC-MS, respectively. Compound I, II and nucleosides are the first report of its occurrence from mustard leaf and their kimchi, the standardized ratios of ingredients for kimchi were 10 of anchovy juice, 8 of red pepper powder, 3 of garlic, 1.5 of ginger, 6 of paste of glutinous rice. The nucleoside of mustard leaf and their kimchi was determined and compared. The order of nucleosides contents of mustard leaf was uridine>cytosine>uracil>adenine>guanosine>guanin, that of fresh mustard leaf kimchi was uridine>uracil>cytosine>guanine>adenosine>adenin>guanosine and that of fermented mustard leaf kimchi (5days at 15$^{\circ}C$) was guanine>adenine>adenosine>guanosine. The differences of nucleoside contents from those were due to various supplements and fermentation process.

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