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http://dx.doi.org/10.15188/kjopp.2015.06.29.3.273

Effects of Vinegar Fermentation of Korean Dendorpanax and Rice Bran Mixture on the Activity of Tyrosinase and Anti-Oxidant in B16F10 Cell Line  

Choi, Chan Hun (College of Korean Medicine, Dongshin University)
An, Jeong Eun (College of Korean Medicine, Dongshin University)
Lim, Song Su (Bitgaram ecofarm community cooperation)
Jeong, Hyun Woo (College of Korean Medicine, Dongshin University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.29, no.3, 2015 , pp. 273-280 More about this Journal
Abstract
In this study, we used the mixture made from the Rice bran 45 ㎏, Dendropanax 5 ㎏, the sugar of the 10% of the total weight, and the enzyme of the 0.1% of the total weight. After the mixture were fermented for 90 days under 20 $^{\circ}C$, we measured the cell viability and the inhibition rate of the melanin biosynthesis, the activity of tyrosinase and superoxide dismutase (SOD) in malignant melanoma, B16F10 cells, in order to survey the whitening effect and the mechanism of the effects on the sample. As a result, the samples significantly suppressed the cell viability of B16F10 in more than 500 ${{\mu}g}$/㎖ and significantly inhibited the generation of melanin induced by ${\alpha}$-MSH in more than 1,000 ${{\mu}g}$/㎖. Sample decreased the activity of tyrosinase while increased the activity of SOD in dose dependent manner. Therefore, we considered that the fermentation extract made from a Rice bran and Dendropanax will be able to produce high value-added products, if used as a commercial.
Keywords
Korean Dendropanax; Rice bran; fermentation; melanin; tyrosinase; SOD;
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