• 제목/요약/키워드: Fer

검색결과 1,525건 처리시간 0.028초

In Vitro Formation of Protein Nanoparticle Using Recombinant Human Ferritin H and L Chains Produced from E. coli

  • RO HYEON SU;PARK HYUN KYU;KIM MIN GON;CHUNG BONG HYUN
    • Journal of Microbiology and Biotechnology
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    • 제15권2호
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    • pp.254-258
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    • 2005
  • We have conducted in vitro reconstitution study of ferritin from its subunits FerH and FerL. For the reconstitution, FerH was produced from an expression vector construct in Escherichia coli and was purified from a heat treated cell extract by using one-step column chromatography. FerL was expressed as inclusion bodies. The denatured form of FerL was obtained by a simple washing step of the inclusion bodies with 3 M urea. The reconstitution experiment was conducted with various molar ratios of urea-denatured FerH and FerL to make the ferritin nanoparticle with a controlled composition of FerH and FerL. SDS-PAGE analysis of the reconstituted ferritins revealed that the reconstitution required the presence of more than 40 molar$\%$ of FerH in the reconstitution mixture. The assembly of the subunits into the ferritin nanoparticle was confmned by the presence of spherical particles with diameter of 10 nm by the atomic force microscopic image. Further analysis of the particles by using a transmission electron microscope revealed that the reconstituted particles exhibited different percentages of population with dense iron core. The reconstituted ferritin nanoparticles made with molar ratios of [FerH]/[FerL]=l00/0 and 60/40 showed that 80 to $90\%$ of the particles were apoferritin, devoid of iron core. On the contrary, all the particles formed with [FerH]/[FerL]=85/ 15 were found to contain the iron core. This suggests that although FerH can uptake iron, a minor portion of FerL, not exceeding $40\%$ at most, is required to deposit iron inside the particle.

Interaction of Nonreceptor Tyrosine-Kinase Fer and p120 Catenin Is Involved in Neuronal Polarization

  • Lee, Seung-Hye
    • Molecules and Cells
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    • 제20권2호
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    • pp.256-262
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    • 2005
  • The neuronal cytoskeleton is essential for establishment of neuronal polarity, but mechanisms controlling generation of polarity in the cytoskeleton are poorly understood. The nonreceptor tyrosine kinase, Fer, has been shown to bind to microtubules and to interact with several actin-regulatory proteins. Furthermore, Fer binds p120 catenin and has been shown to regulate cadherin function by modulating cadherin-${\beta}$-catenin interaction. Here we show involvement of Fer in neuronal polarization and neurite development. Fer is concentrated in growth cones together with cadherin, ${\beta}$-catenin, and cortactin in stage 2 hippocampal neurons. Inhibition of Fer-p120 catenin interaction with a cell-permeable inhibitory peptide (FerP) increases neurite branching. In addition, the peptide significantly delays conversion of one of several dendrites into an axon in early stage hippocampal neurons. FerP-treated growth cones also exhibit modified localization of the microtubule and actin cytoskeleton. Together, this indicates that the Fer-p120 interaction is required for normal neuronal polarization and neurite development.

고분자 전해질 연료전지의 전해질 막 두께가 내구성과 성능에 미치는 영향 (The Effect of Membrane Thickness on Durability and Performance of Proton Exchange Membrane Fuel Cell)

  • 황병찬;이혜리;박권필
    • Korean Chemical Engineering Research
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    • 제55권4호
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    • pp.473-477
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    • 2017
  • 고분자 전해질 연료전지(PEMFC)의 고분자 막은 PEMFC 성능과 내구성에 많은 영향을 준다. 본 연구에서는 고분자막의 두께가 성능과 내구성에 미치는 영향을 파악하기 위해 두께가 다른 Nafion 막의 수소투과도, 불소 유출 속도(FER), 수명, 성능을 측정했다. 막 두께에 따른 수소투과도, 수소투과도와 FER과의 관계, FER과 수명과의 관계로부터 막 두께와 수명의 관계를 얻었다. 막이 두꺼워지면 수소투과도와 FER이 작아지면서 수명이 증가하였다. 반면에 막이 두꺼워지면 막 저항이 증가하면서 성능은 감소하였다. 성능과 내구성을 동시에 만족시키는 막 두께 범위는 $25{\sim}28{\mu}m$였다.

불소함유 에폭시 수지의 합성, 경화 거동 및 유변학적 특성 (Synthesis, Cure Behavior, and Rheological Properties of Fluorine-Containing Epoxy Resins)

  • 박수진;김범용;이재락;신재섭
    • 폴리머
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    • 제27권3호
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    • pp.176-182
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    • 2003
  • 피리딘을 촉매로 사용하여 2-chloro-$\alpha$,$\alpha$,$\alpha$-trifluorotoluene과 glycerol diglycidyl ether를 반응시켜 불소함유 에폭시 수지인 2-trifluorotoluene diglycidylether (FER)을 합성하였다. FER/DM 시스템의 격화 거동은 동적 DSC와 등온 DSC 열분식을 통하여 알아보았으며, Flynn-Wall-Ozawa식을 사용하여 경화 활성화 에너지 (Ea)를 계산하였다. 또한, 본 시스템의 유변학적 특성은 레오미터를 이용하여 등온 조건하에서 고찰하였으며, Arrhenius식을 사용하여 젤화 시간과 경화 온도에 의해 가교 활성화 에너지 (Ec)를 구하였다. 실험 결과, FT-IR, $^{13}$C NMR, 그리고 $^{19}$ F NMR 분광법 분석을 통하여 합성한 수지의 화학 구조를 확인하였으며, FER/DDM 시스템의 Ea는 53.4 kJ/mol이었으며, 경화 반응의 전환율과 전환 속도는 경화 온도가 높을수록 높은 값을 나타내었다. 시스템의 Ec는 41.6 kJ/mol이었으며, 경화 온도가 높을수록 젤화 시간이 단축되었다.

UPPERS TO ZERO IN POLYNOMIAL RINGS OVER GRADED DOMAINS AND UMt-DOMAINS

  • Hamdi, Haleh;Sahandi, Parviz
    • 대한수학회보
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    • 제55권1호
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    • pp.187-204
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    • 2018
  • Let $R={\bigoplus}_{{\alpha}{\in}{\Gamma}}\;R_{\alpha}$ be a graded integral domain, H be the set of nonzero homogeneous elements of R, and ${\star}$ be a semistar operation on R. The purpose of this paper is to study the properties of $quasi-Pr{\ddot{u}}fer$ and UMt-domains of graded integral domains. For this reason we study the graded analogue of ${\star}-quasi-Pr{\ddot{u}}fer$ domains called $gr-{\star}-quasi-Pr{\ddot{u}}fer$ domains. We study several ring-theoretic properties of $gr-{\star}-quasi-Pr{\ddot{u}}fer$ domains. As an application we give new characterizations of UMt-domains. In particular it is shown that R is a $gr-t-quasi-Pr{\ddot{u}}fer$ domain if and only if R is a UMt-domain if and only if RP is a $quasi-Pr{\ddot{u}}fer$ domain for each homogeneous maximal t-ideal P of R. We also show that R is a UMt-domain if and only if H is a t-splitting set in R[X] if and only if each prime t-ideal Q in R[X] such that $Q{\cap}H ={\emptyset}$ is a maximal t-ideal.

인터넷 지역 방송에서 데이터 프레임 길이와 복수 전송에 관한 연구 (A Study on the Data Frame Length and Repetitive Transmission for IP Local Broadcasting)

  • 오종택
    • 한국인터넷방송통신학회논문지
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    • 제11권3호
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    • pp.123-126
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    • 2011
  • 스마트폰과 스마트패드의 사용자가 급격하게 증가됨에 따라 무선랜 AP의 설치가 확대되고 있다. 따라서 향후에 무선랜을 이용한 인터넷 지역 방송 서비스가 활성화될 것이다. 그러나 다수의 단말기로 단방향으로 데이터를 전송하는 방송 기술의 특성상 전송 오류가 발생하는 문제가 발생한다. 본 논문에서는 BER(Bit Error Rate)과 데이터 프레임 길이에 따른 데이터 프레임 오류율(FER: Frame Error Rate)에 대해 분석하여 방송 환경에 따른 적절한 데이터 프레임 길이를 제시하며, 동일한 방송 데이터 프레임을 여러 번 전송하여 실제적으로 FER을 줄이는 방법을 제안한다.

ON THE NUMBER OF SEMISTAR OPERATIONS OF SOME CLASSES OF PRUFER DOMAINS

  • Mimouni, Abdeslam
    • 대한수학회보
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    • 제56권6호
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    • pp.1485-1495
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    • 2019
  • The purpose of this paper is to compute the number of semistar operations of certain classes of finite dimensional $Pr{\ddot{u}}fer$ domains. We prove that ${\mid}SStar(R){\mid}={\mid}Star(R){\mid}+{\mid}Spec(R){\mid}+ {\mid}Idem(R){\mid}$ where Idem(R) is the set of all nonzero idempotent prime ideals of R if and only if R is a $Pr{\ddot{u}}fer$ domain with Y -graph spectrum, that is, R is a $Pr{\ddot{u}}fer$ domain with exactly two maximal ideals M and N and $Spec(R)=\{(0){\varsubsetneq}P_1{\varsubsetneq}{\cdots}{\varsubsetneq}P_{n-1}{\varsubsetneq}M,N{\mid}P_{n-1}{\varsubsetneq}N\}$. We also characterize non-local $Pr{\ddot{u}}fer$ domains R such that ${\mid}SStar(R){\mid}=7$, respectively ${\mid}SStar(R){\mid}=14$.

DaHae: 일한 기계번역을 위한 일본어 형태소 분석기 (DaHae: Japanese Morphological Analyzer for Japanese to Korean Machine Translation)

  • 여상화;정한민;장원;김태완;황도삼;박동인
    • 한국정보과학회 언어공학연구회:학술대회논문집(한글 및 한국어 정보처리)
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    • 한국정보과학회언어공학연구회 1995년도 제7회 한글 및 한국어 정보처리 학술대회
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    • pp.195-207
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    • 1995
  • 일본어는 한자, 히라가나, 가다가나 등 다양한 종류의 문자를 사용하며 이들의 혼용 비율이 매우 높아 띄어쓰기를 하지 않아도 문서의 가독성을 유지한다. ICOT 사전, EDR 사전, ATLAS I/JK사전 등 기존의 전자 사전에서 복합 자종의 표제어가 차지하는 비율(한자+히라가나의 표제어 제외)은 평균 8.8%로 그 수가 매우 작다. 따라서, 문장 내에서 자종의 변화는 단어를 구분하는 하나의 delimiter로 이용될 수 있다. 본 시스템에서는 형태소 분석의 전단계로 전처리기를 두어 자종정보(character type information)에 의한 fragment 분리 및 예외 단어, 정형표현 처리를 수행하며 각 fragment 의 형태소 분석 방법을 제시한다. 형태소 분석기는 전처리기의 처리 결과를 입력받아 각각의 fragment를 전처리기가 제시한 분석 방법에 따라 분석하여 입력 문장의 가능한 모든 분석을 추출한다. 이 방법은 불필요한 사전 탐색과 접속 체크 회수를 줄여 분석 성능을 향상시킨다.

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자돈에서 Sus scrofa ferritin Heavy-chain 생산 재조합 효모의 효과 (Effect of recombinant yeast producing Sus scrofa ferritin Heavy-chain on piglets)

  • 최영준;임환;김현철;김종택;이기종;정배동
    • 한국동물위생학회지
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    • 제35권4호
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    • pp.307-312
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    • 2012
  • Iron deficiency anemia is also recognized as a serious disorder in many livestock, especially, piglets. We previously studied that the iron-fortified yeast (Saccharomyces cerevisiae) producing Sus scrofa ferritin heavy-chain (FER) was bioavailable to mice with iron deficiency. In this study, we determined whether FER could improve iron deficiency in piglets. The bioavailability of FER was examined by measuring body weight gain, hemoglobin concentration and hematocrit value in suckling and weaning piglets. We found that FER significantly increased hemoglobin value and the hematocrit ratio in suckling piglets (P<0.05). Furthermore, FER treatment significantly enhanced body weight gain in both groups of the suckling and weaning piglets (P<0.05). These results suggest that the iron-fortified recombinant yeast strain is helpful in iron absorption in piglets.

A study on the effectiveness of intermediate features in deep learning on facial expression recognition

  • KyeongTeak Oh;Sun K. Yoo
    • International journal of advanced smart convergence
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    • 제12권2호
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    • pp.25-33
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    • 2023
  • The purpose of this study is to evaluate the impact of intermediate features on FER performance. To achieve this objective, intermediate features were extracted from the input images at specific layers (FM1~FM4) of the pre-trained network (Resnet-18). These extracted intermediate features and original images were used as inputs to the vision transformer (ViT), and the FER performance was compared. As a result, when using a single image as input, using intermediate features extracted from FM2 yielded the best performance (training accuracy: 94.35%, testing accuracy: 75.51%). When using the original image as input, the training accuracy was 91.32% and the testing accuracy was 74.68%. However, when combining the original image with intermediate features as input, the best FER performance was achieved by combining the original image with FM2, FM3, and FM4 (training accuracy: 97.88%, testing accuracy: 79.21%). These results imply that incorporating intermediate features alongside the original image can lead to superior performance. The findings can be referenced and utilized when designing the preprocessing stages of a deep learning model in FER. By considering the effectiveness of using intermediate features, practitioners can make informed decisions to enhance the performance of FER systems.