• Asian-Australasian Journal of Animal Sciences
• /
• 제13권2호
• /
• pp.188-191
• /
• 2000

Appetite stimulant ether-soluble neutral extract of a high-grain compound feed was separated into four fractions with an open column of silica gel chromatograph using four carrier solutions containing n-pentane and diethyl ether as 100:0 (Fraction A), 90:10 (Fraction B), 75:25 (Fraction C) and 0:100 (Fraction D). The stimulative effects of the fractions were examined by comparing the intake of hay (with or without the fractions) in two-choice test bioassays with cattle. The Fractions A, B and C of the neutral extract stimulated (p<0.05) hay consumption in cattle, whereas Fraction D had no effect on selective feeding when compared with the control fraction. Furthermore, Fractions A, B and C had higher (p<0.05) feeding stimulative indices (FSI) than that of the Fraction D. The results suggest that chemical stimulants to increase palatability of hay are present in the neutral Fractions A, B and C of high-grain concentrate.

• 환경생물
• /
• 제31권4호
• /
• pp.458-470
• /
• 2013

Haliotis discus, a useful abalone of herbivorous gastropod, shows feeding preference to marine algae depending upon their growth stage and recognition of taste. This study was carried out to investigate this abalone's algal preferences and the presence of feeding stimulants. In single-choice experiments the small (S) group generally preferred Ulva pertusa (Chlorophyta), whereas the medium (M) and large (L) group preferred both Laminaria japonica (Phaeophyta) and Undaria pinnatifida (Phaeophyta). In multi-choice experiments using 4 algal species of L. japonica, U. pertusa, U. pinnatifida and Ecklonia cava (Phaeophyta), the results were same as in the single-choice experiments; the S group preferred U. pertusa the most, while the M and L group preferred both U. pinnatifida and L. japonica. However E. cava was not preferred by any groups. In order to examine the presence of feeding stimulant, chemical compounds from algae used as feed were isolated and identified. The abalone responded to water soluble matters of L. japonica, U. pinnatifida and U. pertusa, but those of E. cava and Sargassum sagamianum (Phaeophyta) were not attractive to them. In feeding stimulant experiments using fat soluble matters, the S group preferred the fat soluble matter of U. pertusa the most, while the M group and the L group preferred those of U. pertusa and U. pinnatifida, and those of L. japonica, respectively. However the fat soluble matter of S. sagamianum was not attractive to the abalone. The results of feeding stimulant experiments were same as those of single-choice or multi-choice experiments, which showed that compound lipids in fat soluble matter might act as feeding-stimulant.

• 한국양식학회지
• /
• 제15권3호
• /
• pp.157-163
• /
• 2002

본 실험은 섭취촉진물질로 알려져 있는 사료내 13종의 아미노산들이 치어기 조피볼락에 있어 성장 및 체조성에 어떠한 영향을 미치는지 알아보고자 실시하였다 실험어는 평균무게 0.64 g인 조피볼락 치어를 사용하였으며, 기초실험 사료내에 유인효과가 있을 것으로 여겨지는 아미노산 13가지(Arg, Ala, Na-Glu, Gly, His, Ile, Lys, Met, Phe, Pro, Thr, Trp 및 Val)를 사료내 각각 0.3% 씩 첨가하여 총 13 실험구를 2반복으로 나누어 6주간 사육하였다. 6주간의 사육실험 결과, 성장률(WG)과 일간 성장률(SGR)에서는 Pro, Thr, Met 및 Gly 실험구가 다른 실험구에 비해 유의적으로 높았으나(P<0.05), 이들 실험구간에는 유의적인 차이가 없었다(P>0.05). 그리고, 사료전환효율(FE)에서는 Pro 실험구가 Met과 Gly 실험구보다 유의적으로 높았으며(P<0.05), Pro 실험구와 Thr 실험구 사이에는 유의적인 차이가 없었다(P>0.05). 그리고 Thr, Met 및 Gly 실험구간에도 유의적인 차이가 없었다(P>0.05). 단백질전화효율에 있어서 Pro 실험구가 모든 실험구에 있어서 유의적으로 가장 높았으며(P<0.05), Thr 실험구와 비교하여 유의적으로 차이가 나지 않았다(P>0.05). 그리고, 비만도(condition factor)는 Thr 실험구가 유의적으로 가장 높았으며(P<0.05), Val 실험구는 가장 낮게 나타났다(P>0.05). 간중량지수(HSI)는 모든 실험구간에서 유의적인 차이가 나지 않았다(P>0.05). 전어체의 단백질, 수분 및 회분에 있어서는 모든 실험구에 있어서 유의적인 차이가 나지 않았다(P>0.05). 지질함량은 Met, Pro 및 Thr 실험구가 Glu, Gly, Lys, Trp 및 Val 실험구보다 유의적으로 낮았다(P>0.05). 상기 결과를 토대로, 조피볼락 사료용 사료섭취촉진물질로서 아미노산을 검색한 결과 사료내 0.3% Pro, Thr, Met 및 Gly을 첨가했을 때 사료섭취촉진 효과가 나타났으며, 이것들 중 Gly을 제외하고는 전어체의 지질 함량을 감소시키는 결과를 나타내었다.

• 농업과학연구
• /
• 제50권3호
• /
• pp.349-356
• /
• 2023

Garlic powder (GP) was supplemented in the diet to assess the growth efficiency, nutritional retention, and fecal score of growing pigs. In total, 80 growing pigs ([Yorkshire × Landrace] × Duroc) were arbitrarily allotted (22.04 ± 0.56 kg primary average body weight) to two different trial groups with eight repetitions (three males and two females) in each pen for the 42-day trial. The dietary treatment consisted of control (CON, basal diet), which included 0.2% GP (TRT1). Growing pigs fed the GP diet exhibited an increase (p < 0.05) in body weight on day 42 and a lower (p < 0.05) overall feed conversion ratio compared to the CON pigs; however, the average daily gain and feed intake showed no difference (p > 0.05). Additionally, nutrient utilization of dry matter was greater (p < 0.05) in the GP-supplemented diet group than in the CON group. The feeding methods did not adversely affect the nitrogen and energy utilization and the fecal score significantly (p > 0.05). In summary, growing pigs fed the GP diet showed increased growth and nutritional utilization, and no adverse impact on the fecal score; thus, it could be utilized as a stimulant for improved growth performance.

• 한국수산과학회지
• /
• 제53권2호
• /
• pp.191-202
• /
• 2020

We evaluated the effects of dietary supplementation of phytosterol on the growth, feed utilization, immunity, digestibility, wound healing ability and disease resistance of olive flounder Paralichthys olivaceus. We conducted two consecutive feeding trials at different growth stages of the fish: EXP-1 (68.9 g) and EXP-2 (16.5 g). The experimental diets were supplemented with graded levels of phytosterol (1% ecdysteroid extracted from Achyranthis radix) at 0, 0.025, 0.05, 0.1, 0.2 and 0.4% (designated as Con, PHY0.025, PHY0.05, PHY0.1, PHY0.2 and PHY0.4, respectively). Dietary phytosterol did not significantly affect growth and cholesterol concentration. Feed utilization was higher in fish fed phytosterol-supplemented diets than in fish fed the control diet. Dietary phytosterol increased innate immunity and digestibility of protein and dry matter. Wound healing ability was also increased by the phytosterol supplementation. The survival against Edwardsiella tarda challenge was higher in fish fed low-phytosterol diets than in fish fed the control diet. The optimum dietary level of phytosterol seems to be approximately 0.05%. The results in this study indicate that the phytosterol could be used as a functional supplement in diets to improve feed utilization, immunity, digestibility and wound healing ability of olive flounder.

• 한국수산과학회지
• /
• 제42권1호
• /
• pp.26-33
• /
• 2009

This experiment was conducted to examine the utilization of added dietary selenium (Se) as an immune stimulant in juvenile olive flounder, Paralichthys olivaceus. Fish averaging $4.0{\pm}0.1\;g$ ($mean{\pm}SD$) were fed one of seven semi-purified diets containing 0.56, 1.07, 2.86, 4.56, 43.15, 90.71, or 161.74 mg of Se/kg ($Se_{0.56}$, $Se_{1.07}$, $Se_{2.86}$, $Se_{4.56}$, $Se_{43.2}$, $Se_{90.7}$ and $Se_{161.7}$, respectively) for 12 weeks, respectively. At the end of the feeding trial, the fish fed diets containing more than 43.2 mg of Se/kg showed above 90% mortality. There were no significant differences in weight gain, feed efficiency, specific growth rate, protein efficiency ratio, or hematological characteristics among the fish fed the $Se_{0.56}$, $Se_{l.07}$, $Se_{2.86}$, and $Se_{4.56}$ diets. Se concentrations of the gill, kidney, muscle and liver tissues occurred in dose-dependent manners. Alternative complement pathway activation and the chemiluminescene responses of the fish fed the $Se_{1.07}$ diet were significantly higher than those of the fish fed the other diets (P<0.05). These results indicate that the optimum dietary supplementation level of Selenium as selenoyeast could be 1.07 mg of Se/kg based on the non-specific immune responses of juvenile oilve flounder, Paralichthys olivaceus.

• 대한수의학회지
• /
• 제55권1호
• /
• pp.1-7
• /
• 2015

Ginseng has been widely used in Korea as a natural medicine due to its saponin contents. Although the total amount of ginseng stem and leaf saponins (GSLS) is 4~5 times higher than that of saponin in the root, the root is mainly used. This is due to two reasons: nervous system-stimulant activity of GSLS and pesticide residues in GSLS. In this study, residual agricultural pesticides were removed from GSLS using two types of bacterial treatments. Two GSLS treatment groups of chickens (GSLS-1 and GSLS-2) were established. The chickens were fed 0.4% GSLS-1 or GSLS-2 mixed with crop. We then evaluated the effects of GSLS on bodyweight and several immune parameters. At the end of the experiments, chickens fed GSLS-1 and red ginseng saponin had significantly higher growth rates (16.6% and 8.0%, respectively) compared to the vaccine control group treated with Noblis Salenvac-T. The group fed GSLS-1 also had the highest IgG titer that was significantly different at the end of experiments compared to the other groups. These findings imply that GSLS-1 is a good candidate feed additive for the chicken industry.

• 한국수산과학회지
• /
• 제56권3호
• /
• pp.300-308
• /
• 2023

This study aimed to evaluate the utilization of low fish meal (LFM) diet and black soldier fly (BSF) Hermetia illucens meal supplementation in LFM diet as a fish meal (FM) replacer or functional additive for juvenile olive flounder Paralichthys olivaceus. Three LFM diets were prepared to contain 30, 25, and 20% FM, respectively (designated as F30, F25, and F20). Three BSF diets were prepared by adding 2.0, 2.5, and 3.0% BSF in the LFM diets, respectively (designated as F30B2.0, F25B2.5, and F20B3.0). A total of 540 fish (initial mean body weight: 22.3±0.01g) were randomly distributed into 18 tanks (240 L) in triplicate groups and fed the experimental diets twice daily for 12 weeks. Results showed that there were no significant differences in the growth performance, feed utilization and survival among all the groups. Lysozyme activity significantly increased in F30B2.0 and F25B2.5 groups compared to their respective LFM groups. Total immunoglobulin was significantly higher in F30 group than in F20 group. Anti-protease and myeloperoxidase activities were significantly higher in F30B2.0 group than in F20 group. Innate immunity gradually decreased with decreasing FM level in all the groups. BSF supplementation significantly improved immunity compared to their respective LFM diets. Therefore, BSF could be used as an immuno-stimulant supplement in LFM diets for P. olivaceus.

• Journal of Animal Science and Technology
• /
• 제48권5호
• /
• pp.663-670
• /
• 2006

국내에서 제조된 서방출형 유전자재조합 돼지 성장호르몬 (porcine somatotropin; pST)이 돼지의 성장과 등지방두께에 미치는 영향을 구명하기 위해 평균체중이 78kg인 랜드레이스 거세돈 48두를 공시하여 대조구, 4:1제형 rpST구 (6회 투여), 1:1제형 rpST구 (4회 투여), 4:1제형 rpST구 (4회 투여)에 각각 12두씩 배치하였다. 대조구는 pST를 투여하지 않았고, rpST 처리구는 1주일에 1회, 목 근육에 서방형 제재를 주입하였는데, 6회 투여구는 개시부터 2주까지는 100mg을, 3주부터 6주까지는 125mg을, 4회 투여구는 개시부터 2주까지는 100mg을, 3주부터 4주까지는 125mg을 투여하였다. 시험사료는 라이신 함량이 0.9%인 시판 육성돈사료를 무제한 급여한 결과 (주) LG 화학에서 제조한 서방출형 유전자재조합 pST는 증체량 및 사료요구율을 개선시켜 생산성 향상과 등지방두께 감소로 육질이 향상되었다.

• 셀메드
• /
• 제11권3호
• /
• pp.13.1-13.9
• /
• 2021

Background and Objective: Nux-vomica based traditional Unani formulation, Habb-e-Azaraqi (HAZ) is an important drug used by Unani physicians since several decades. It possesses Muqawwi-i-A'sab (nervine tonic), Muharrik-i-A'sab (nervine stimulant) properties and is an effective treatment option for diseases like Laqwa (facial palsy), Falij (paralysis), Niqris (gout) and Waja'al-Mafasil (arthritis) etc. The aim of the study is to access and provide information of HAZ for its TLC, HPTLC Fingerprinting defining its clear qualitative perspective and acute oral toxicity evaluation for its safety assessment which was not done earlier, thus contributing in the field of research. Materials and Methods: The chief ingredient, nux-vomica was detoxified as per method mentioned in Unani Pharmacopeia before its use in formulation. TLC and HPTLC was developed under four detection system i.e., UV 366nm, UV 254nm, exposure to iodine vapours and after derivatization with anisaldehyde sulphuric acid. Acute toxicity studies were performed as per OECD Guidelines 425 at a limit dose of 2000 mg/kg. Observations were done for signs of toxicity, body weight, and feed consumption at regular intervals followed by haematological and biochemistry evaluation. Results: The generated data proved the authenticity and established the TLC and HPTLC profile of the formulation. Acute toxicity revealed no significant differences in HAZ-treated animals with respect to body weight gain, feed consumption, haematology, clinical biochemistry evaluation. No significant gross pathological observation was noticed in necropsy. Conclusion: Data of the present study is substantial and scientific proof of HAZ in terms of standardization and toxicity study that can be utilize in future research activities.