• Title/Summary/Keyword: Fecal examination

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Blood Chemical Values of Fascioliasis in Holstein Cow (간질에 감염된 젖소의 혈액화학치에 관한 연구)

  • Kim Tae-Jong;Lee Won-Chang;Kim Hak-Jae;Song Kee-Hoe
    • Journal of the korean veterinary medical association
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    • v.26 no.4
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    • pp.226-231
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    • 1990
  • In order to investigate the infectious rate of the internal parasites, A total of nine hundred and five head of Holstein cow in the area of Kyungi Do was randomly selected for the fecal examination. And the chemical values of blood were examined from 20 H

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Comparative Study on the Effectiveness of Modified Kato's Cellophane Thick Smear and Stoll's Dilution Egg Counting Technique for Quantitative Fecal Examination of Helminth Eggs (윤충란 정량검사를 위한 Stoll 희석충란계산법과 Kato 후층도말변법의 비교)

  • 홍성종;우호춘
    • Parasites, Hosts and Diseases
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    • v.30 no.2
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    • pp.141-146
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    • 1992
  • A total of 197 fecal specimens was prepared for quantitative examination of helminth eggs by modified Kato's cellophane thick smear (M.C.T.S.) and Stoll's dilution egg counting technique (D.E,C.T.). The comparative effectiveness of two techniques was evaluated and conversion function was deduced. The average time required for the microscopic examination on one slide by M.C.T.S. was 12.6 minutes and that of D.E.C.T. was 14.6 minutes. M.C.T.S. showed lower false negative rate than D.E.C.T in light worm burden cases. Functions to convert the counts obtained by M.C.T.S. to E.P.G. by Stoll's dilution egg counting technique were $47.86{\times}10^{0.87{\;}logM.C.T.S.},{\;}$ in A. lumbricoides, $41.69{\times}10^{0.82{\;}logM.C.T.S.}$ in T. trichiura and $63.10{\times}10^{0.85{\;}logM.C.T.S.}$ in C. sinensis. It was suggested M.C.T.S. be better than D.E.C.T. for the quantitative examination of intestinal helminthiases such as A. lumbricoides, T. trichiura, and C. sinensis infections even in the cases with low worm burden.

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Gastritis in a Dog Infected with Helicobacter heilmannii (Helicobacter heilmannii 균 감염이 확인된 개에서의 위장염 발생 증례)

  • 황철용;윤화영;한홍율
    • Journal of Veterinary Clinics
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    • v.19 no.3
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    • pp.375-378
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    • 2002
  • A 3-year-old intact female korean pungsan dog showing chronic intermittent vomiting and anorexia was admitted to The Veterinary Medical leaching Hospital of Seoul National University. The patient had been received antiemetics, antacids or proton pump inhibitor for these symptoms but had not been recovered yet. All results of physical examination, complete blood count, serum chemical analysis, fecal test and radiography were normal. Gastric endoscopic examination was also performed and gastritis with hemorrhage on near antrum region was detected. In PCR assay of bioptic gastric tissues for detecting Helicobacter species infection, Helicobacter heilmannii infection was confirmed. After receiving triple therapy with amoxicillin, metronidazole and omeprazole for 21 days, all symptoms were recovered and had not been occurred again. Helicobacter heilmannii also were not detected.

Prevalence of Fascioliasis of Korean Native Cattle in Kangwon Province in Korea (강원도 사육 한우의 간질 감염실태)

  • Kim, Yeon-Soo;Kim, Sang-Kyun;Hwang, Eui-Kyung
    • Korean Journal of Veterinary Research
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    • v.41 no.4
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    • pp.557-563
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    • 2001
  • A field survey of fascioliasis of Korean native cattle raising and raised in specialized commercial breeding farms and local farms in Kangwon province using both intradermal test and sedimentation technique for feces was carried out from November to December, 1996. Fecal samples were taken from fascioliasis positive cattle by the intradermal test for the fecal examination. Liver tissues were randomly collected from an abattoir for histopathological examination of liver fluke infection in cattle. The results are as follows. 1. By the intradermal test for a total of 211 cattle raising in both Wonju and Wheongsung, Kangwon province, 60 heads(28.4%) showed positive reaction. Among 60 positive cattle, eggs of Fasciola hepatica were found from 51 heads(85.0%) by sedimentation technique. 2. According to the cattle raising areas, the positive rates by the intradermal test were 26.7%(20 out of 75 heads) in Wonju and 29.4%(40 out of 136 heads) in Wheongsung. 3. According to the age of cattle examined, the positive rates by the intradermal test in 1~3, 4~6 and 7~10 years old were 11.7%(7 out of 100 heads), 68.3%(41 out of 93 heads) and 20.0%(12 out of 18 heads), respectively. 4. The overall infection rates of fluke larvae from the slaughtered cattle at an abattoir in Wonju was 24.7%(37 out of 150 heads). In histopathology, liver lesions were observed such as inflammation with infiltration of eosinophils, polymorphonuclear cells, mononuclear cells and multinucleated giant cells, proliferation of connective tissues, calcification and abscess formation.

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A successful experience of soil-transmitted helminth control in the Republic of Korea

  • Hong Sung-Tae;Chai Jong-Yil;Choi Min-Ho;Huh Sun;Rim Han-Jong;Lee Soon-Hyung
    • Parasites, Hosts and Diseases
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    • v.44 no.3
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    • pp.177-185
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    • 2006
  • Soil-transmitted helminths (STH), namely Ascaris, Trichuris and hookworms (Ancylostoma and Necator), present a global health problem to about a half of the earth's population. In the Republic of Korea, STH were highly prevalent and were considered a high priority target for national control. To promote the control, a non-governmental organization named Korea Association for Parasite Eradication (currently Korea Association of Health Promotion) was founded in 1964, and mass fecal examination followed by selective mass chemotherapy with anthelmintics was performed twice a year from 1969 to 1995 targeting whole nationwide schoolchildren. Meanwhile, decreasing patterns of national STH infections have been monitored by 7 times' quinquennial national surveys targeting general population. In 1971, the overall intestinal helminth egg positive rate was 84.3% (Ascaris 58.2%, Trichuris 65.4%, and hookworms 10.7%), which became 63.2% in 1976, 41.1% in 1981, 12.9% in 1986, 3.8% in 1992, 2.4% in 1997, and 4.3% (Ascaris 0.03%, Trichuris 0.02%, and hookworms 0%) in 2004. During the control period, national economy rapidly developed, and living standards including environment, sanitation, and agricultural technology greatly improved, which undoubtedly boosted the STH control effects. Our experience indicates that social driving force to establish an eligible national control system to conduct repeated mass chemotherapy, together with improvement of environment and sanitation, is important for initiating and achieving STH control in a developing community.

Isolation of Pichia burtonii from the Feces of an Enteritis Bearded Dragon (Pogona vitticeps) (장염을 나타낸 bearded dragon의 분변에서 Pichia burtonii의 분리)

  • Kang, Hyo-Min;Han, Jae-Ik;Lee, Sook-Jin;Jang, Hye-Jin;Na, Ki-Jeong
    • Journal of Veterinary Clinics
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    • v.28 no.2
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    • pp.254-257
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    • 2011
  • A 2-year-old bearded dragon was referred to the Veterinary Medical Center at the College of Veterinary Medicine, Chungbuk National University with reduced activity and anorexia. On fecal examination, over growth of a bacteria and the proliferation of a yeast-like organism were found. The patient diagnosed with enteritis. By using fungal cultures and molecular typing, the yeast was identified as Pichia (P.) burtonii. The bearded dragon was treated with oral ketoconazole and trimethoprim/sulfamethoxazole. After 3 days, the dragon was recovered and fecal examination showed that the yeast had disappeared from the feces. The strain P. burtonii is supposed opportunistic pathogen in bearded dragon with enteritis according to its reports in a human. This report is the first paper about overgrowth of P. burtonii in a bearded dragon.

Morphological and Molecular Diagnosis of Necator americanus and Ancylostoma ceylanicum Recovered from Villagers in Northern Cambodia

  • Chang, Taehee;Jung, Bong-Kwang;Sohn, Woon-Mok;Hong, Sooji;Shin, Hyejoo;Ryoo, Seungwan;Lee, Jeonggyu;Lee, Keon Hoon;Khieu, Virak;Huy, Rekol;Chai, Jong-Yil
    • Parasites, Hosts and Diseases
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    • v.58 no.6
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    • pp.619-625
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    • 2020
  • Human hookworm infections caused by adult Ancylostoma spp. and Necator americanus are one of the most important tropical diseases. We performed a survey of intestinal helminths using the Kato-Katz fecal examination technique targeting 1,156 villagers residing in 2 northern provinces (Preah Vihear and Stung Treng) of Cambodia in 2018. The results revealed a high overall egg positive rate of intestinal helminths (61.9%), and the egg positive rate of hookworms was 11.6%. Nine of the hookworm egg positive cases in Preah Vihear Province were treated with 5-10 mg/kg pyrantel pamoate followed by purging with magnesium salts, and a total of 65 adult hookworms were expelled in diarrheic stools. The adult hookworms were analyzed morphologically and molecularly to confirm the species. The morphologies of the buccal cavity and dorsal rays on the costa were observed with a light microscope, and the nucleotide sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene were analyzed. The majority of the hookworm adults (90.7%) were N. americanus, whereas the remaining 9.3% were Ancylostoma ceylanicum, a rare hookworm species infecting humans. The results revealed a high prevalence of hookworm infections among people in a northern part of Cambodia, suggesting the necessity of a sustained survey combined with control measures against hookworm infections.

Infection rate of parasites from feces of Korean indigenous goats in northern areas of Gyeongbuk province (경북 북부지역 재래산양 분변에서의 기생충 감염률 조사)

  • Cho, Kwang-Hyun;Lee, Ji-Hong;Kim, Dae-Jung;Kim, Seung-Joon;Kwon, Oh-Deog;Kwak, Dong-Mi
    • Korean Journal of Veterinary Service
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    • v.31 no.3
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    • pp.357-362
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    • 2008
  • While studies have been carried out on endoparasite infection from feces of Korean indigenous goats in multiple areas around the nation, there is no report in northern areas of Gyeongbuk province. Thus, this study was conducted to examine the rate of parasite infection from the fecal samples of goats raised on grass. From March to July in 2008, 108 fecal samples were taken from goats. Eggs of parasites were identified using flotation or sedimentation methods followed by microscopic examination. Overall infection rate of parasites from feces of goats were 99% (107 out of 108). The infection rates of nematode, cestode, trematode and protozoa were 37%, 23%, 0% and 99%, respectively. Among parasite eggs detected, nematodes included threadworms (20%), strongyles (16%) and whipworms (1%). The only cestode and protozoa detected were Moniezia expansa and Eimeria spp, respectively. In the rates of mixed infection, single was 61%, double 32%, and triple 6%.

Identification of Cystoisospora ohioensis in a Diarrheal Dog in Korea

  • Lee, Sangmin;Kim, Junki;Cheon, Doo-Sung;Moon, Eun-A;Seo, Dong Joo;Jung, Soontag;Shin, Hansaem;Choi, Changsun
    • Parasites, Hosts and Diseases
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    • v.56 no.4
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    • pp.371-374
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    • 2018
  • A 3-month-old female Maltese puppy was hospitalized with persistent diarrhea in a local veterinary clinic. Blood chemistry and hematology profile were analyzed and fecal smear was examined. Diarrheal stools were examined in a diagnostic laboratory, using multiplex real-time polymerase chain reaction (PCR) against 23 diarrheal pathogens. Sequence analysis was performed using nested PCR amplicon of 18S ribosomal RNA. Coccidian oocysts were identified in the fecal smear. Although multiplex real-time PCR was positive for Cyclospora cayetanensis, the final diagnosis was Cystoisospora ohioensis infection, confirmed by phylogenetic analysis of 18S rRNA. To our knowledge, this the first case report of C. ohioensis in Korea, using microscopic examination and phylogenetic analysis.

Distribution of Gastrointestinal Parasitic Infection in Domestic Pigs in the Republic of Korea: Nationwide Survey from 2020-2021

  • Lee, Subin;Alkathiri, Badriah;Kwak, Dongmi;Lee, Sang-Myeong;Lee, Wan-Kyu;Byun, Jae-Won;Lee, Seung-Hun
    • Parasites, Hosts and Diseases
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    • v.60 no.3
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    • pp.207-211
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    • 2022
  • This study aimed to examine the distribution of gastrointestinal parasitic infections in domestic pigs in the Republic of Korea. From May 2020 to October 2021, 364 pig fecal samples were collected from 75 farms in 7 Provinces and microscopically examined. A total of 170 (46.7%) pigs were infected with at least one of the following parasites: Balantioides coli, strongyles, Ascaris suum, Trichuris suis, and coccidia. By parasite species, B. coli, strongyles, A. suum, T. suis, and coccidia oocysts or eggs were detected in 144 (39.6%), 24 (6.6%), 14 (3.8%), 4 (1.1%), and 1 (0.3%) samples, respectively. One hundred fifty-four, 15, and 1 cases showed single, double, and triple infections, respectively. Of the swine fecal samples from 75 farms, 69 specimens (92.0%) were infected with 1 or more parasites. All surveyed farms across the country exhibited a positive rate of over 30%, among which the highest positive rate was 65.0% in Chungcheongnamdo, and Jeollabuk-do was followed by 61.9%. Winter showed a statistically lower prevalence than other seasons. This study showed that gastrointestinal parasites are prevalent in pigs in Korea, although the diversity of parasites is low.