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Isolation of Pichia burtonii from the Feces of an Enteritis Bearded Dragon (Pogona vitticeps)  

Kang, Hyo-Min (Veterinary Laboratory Medicine, College of Veterinary Medicine, Veterinary Medical Center, Chungbuk National University)
Han, Jae-Ik (Veterinary Laboratory Medicine, College of Veterinary Medicine, Veterinary Medical Center, Chungbuk National University)
Lee, Sook-Jin (Veterinary Laboratory Medicine, College of Veterinary Medicine, Veterinary Medical Center, Chungbuk National University)
Jang, Hye-Jin (Veterinary Laboratory Medicine, College of Veterinary Medicine, Veterinary Medical Center, Chungbuk National University)
Na, Ki-Jeong (Veterinary Laboratory Medicine, College of Veterinary Medicine, Veterinary Medical Center, Chungbuk National University)
Publication Information
Journal of Veterinary Clinics / v.28, no.2, 2011 , pp. 254-257 More about this Journal
Abstract
A 2-year-old bearded dragon was referred to the Veterinary Medical Center at the College of Veterinary Medicine, Chungbuk National University with reduced activity and anorexia. On fecal examination, over growth of a bacteria and the proliferation of a yeast-like organism were found. The patient diagnosed with enteritis. By using fungal cultures and molecular typing, the yeast was identified as Pichia (P.) burtonii. The bearded dragon was treated with oral ketoconazole and trimethoprim/sulfamethoxazole. After 3 days, the dragon was recovered and fecal examination showed that the yeast had disappeared from the feces. The strain P. burtonii is supposed opportunistic pathogen in bearded dragon with enteritis according to its reports in a human. This report is the first paper about overgrowth of P. burtonii in a bearded dragon.
Keywords
bearded dragon; enteritis; Pichia burtonii;
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1 Van Burik JA, Schreckhise RW, White TC, Bowden RA, Myerson D. Comparison of six extraction techniques for isolation of DNA from filamentous fungi. Med Mycol 1998; 36: 299-303.
2 Villa-Carvajal M, Querol A, Belloch C. Identification of species in the genus Pichia by restriction of the internal transcribed spacers (ITSI and ITS2) and the 5.8S ribosomal DNA gene. Antonie Van Leeuwenhoek 2006; 90: 171-181.   DOI   ScienceOn
3 Wamsley HL. Dry-mount fecal cytology. In: Canine and feline cytology: A color atlas and interpretation guide. St. Louis: Saunders. 2010: 215-225.
4 Otag F, Kuyucu N, Erturan Z, Sen S, Emekdas G, Sugita T. An outbreak of Pichia ohmeri infection in the paediatric intensive care unit: case reports and review of the literature. Mycoses 2005; 48: 265-269.   DOI   ScienceOn
5 Takeuchi A, Shimizu-Ibuka A, Nishiyama Y, Mura K, Okada S, Tokue C, Arai S. Purification and characterization of an alpha-amylase of Pichia burtonii isolated from the traditional starter "murcha" in Nepal. Biosci Biotechnol Biochem 2006; 70: 3019-3024   DOI   ScienceOn
6 Kostka VM, Hoffmann L, Balks E, Eskens U, Wimmershof N. Review of the literature and investigations on the prevalence and consequences of yeasts in reptiles. Vet Rec 1997; 140: 282-287.   DOI   ScienceOn
7 Makimura K, Mochizuki T, Hasegawa A, Uchida K, Saito H, Yamaguchi H. Phylogenetic classification of Trichophyton mentagrophytes complex strains based on DNA sequences of nuclear ribosomal internal transcribed spacer 1 regions. J Clin Microbiol 1998; 36: 2629-2633.
8 Nardoni S, Papini R, Marcucci M, Mancianti F. Survey on the fungal flora of the cloaca of healthy pet reptiles. Revue Med Vet 2008; 159: 159-165.
9 Esteve-Zarzoso B, Belloch C, Uruburu F, Querol A. Identification of yeasts by RFLP analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers. Int J Syst Bacteriol 1999; 49: 329-337.   DOI   ScienceOn
10 Funk RS. Anorexia. ln: Reptile medicine and surgery. Philadelphia: Saunders. 1996: 346-348
11 Cuero RG, Smith JE, Lacey J. Stimulation by Hyphopichia burtonii and Bacillus amyloliquefaciens of aflatoxin production by Aspergillus flavus in irradiated maize and rice grains. Appl Environ Microbiol 1987; 53: 1142-1146.
12 Bakir M, Cerikcioglu N, Tirtir A, Berrak S, Ozek E, Canpolat C. Pichia anomala fungaemia In Immunocompromised children. Mycoses 2004; 47: 231-235   DOI   ScienceOn