• KSII Transactions on Internet and Information Systems (TIIS)
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• 제15권11호
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• pp.4224-4243
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• 2021

Cognitive radio (CR) is a feasible intelligent technology and can be used as an effective solution to spectrum scarcity and underutilization. As the key function of CR, cooperative spectrum sensing (CSS) is able to effectively prevent the harmful interference with primary users (PUs) and identify the available spectrum resources by exploiting the spatial diversity of multiple secondary users (SUs). However, the open nature of the cognitive radio networks (CRNs) framework makes CSS face many security threats, such as, the malicious user (MU) launches Byzantine attack to undermine CRNs. For this aim, we make an in-depth analysis of the motive and purpose from the MU's perspective in the interweave CR system, aiming to provide the future guideline for defense strategies. First, we formulate a dynamic Byzantine attack model by analyzing Byzantine behaviors in the process of CSS. On the basis of this, we further make an investigation on the condition of making the fusion center (FC) blind when the fusion rule is unknown for the MU. Moreover, the throughput and interference to the primary network are taken into consideration to evaluate the impact of Byzantine attack on the interweave CR system, and then analyze the optimal strategy of Byzantine attack when the fusion rule is known. Finally, theoretical proofs and simulation results verify the correctness and effectiveness of analyses about the impact of Byzantine attack strategy on the throughput and interference.

• Journal of Microbiology and Biotechnology
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• 제14권1호
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• pp.81-89
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• 2004

Tumor necrosis factor-$\alpha$ (TNF-$\alpha$) and lymphotoxin-$\alpha$ (LT-$\alpha$, TNF-$\beta$) can initiate and perpetuate human diseases such as multiple sclerosis (MS), rheumatoid arthritis (RA), and insulin-dependent diabetes mellitus (IDDM). TNFs can be blocked by the use of soluble TNF receptors. However, since monomeric soluble receptors generally exhibit low affinity or function as agonists, the use of monomeric soluble receptors has been limited in the case of cytokines such as TNF-$\alpha$, TNF-$\alpha$, interleukin (IL)-1, IL-4, IL-6, and IL-13, which have adapted to a multi component receptor system. For these reasons, very high-affinity inhibitors were created for the purpose of a TNFs antagonist to bind the TNFR and trigger cellular signal by using the multistep polymerase chain reaction method. First, recombinant simple TNFR-Ig fusion proteins were constructed from the cDNA sequences encoding the extracellular domain of the human p55 TNFR (CD120a) and the human p75 TNFR (CD120b), which were linked to hinge and constant regions of human $IgG_1$ heavy chain, respectively using complementary primers (CP) encoding the complementary sequences. Then, concatameric TNFR-Ig fusion proteins were constructed using recombinant PCR and a complementary primer base of recombinant simple TNFR-Ig fusion proteins. For high level expression of recombinant fusion proteins, Chinese hamster ovary (CHO) cells were used with a retroviral expression system. The transfected cells produced the simple concatameric TNFR-Ig fusion proteins capable of binding TNF and inactivating it. These soluble versions of simple concantameric TNFR-Ig fusion proteins gave rise to multiple forms such as simple dimers and concatameric homodimers. Simple TNFR-1g fusion proteins were shown to have much more reduced TNF inhibitory activity than concatameric TNFR-Ig fusion proteins. Concatameric TNFR-Ig fusion proteins showed higher affinity than simple TNFR-Ig fusion proteins in a receptor inhibitor binding assay (RIBA). Additionally, concatameric TNFR-Ig fusion proteins were shown to have a progressive effect as a TNF inhibitor compared to the simple TNFR-Ig fusion proteins and conventional TNFR-Fc in cytotoxicity assays, and showed the same results for collagen induced arthritis (CIA) in mice in vivo.

• BMB Reports
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• 제43권8호
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• pp.541-546
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• 2010

We utilized a mammalian expression system to purify and characterize autotaxin (ATX)/lysophospholipase D, an enzyme present in the blood responsible for biosynthesis of lysophosphatidic acid. The human ATX cDNA encoding amino acids 29-915 was cloned downstream of a secretion signal of CD5. At the carboxyl terminus was a thrombin cleavage site followed by the constant domain (Fc) of IgG to facilitate protein purification. The ATX-Fc fusion protein was expressed in HEK293 cells and isolated from conditioned medium of a stable clone by affinity chromatography with Protein A sepharose followed by cleavage with thrombin. The untagged ATX protein was further purified to essential homogeneity by gel filtration chromatography with a yield of approximately 5 mg/liter medium. The purified ATX protein was enzymatically active and biologically functional, offering a useful tool for further biological and structural studies of this important enzyme.

• IMMUNE NETWORK
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• 제7권4호
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• pp.203-208
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• 2007

Background: Angiogenesis mediated by VEGF constitutes a new target for anti-cancer therapy which has explored through different ways of intervention aiming at the blocking of the tumoral angiogenesis. In the present study, we developed the assays by which efficacies of anti-VEGF inhibitor candidates are evaluated at the various levels. Methods & Results: First, we developed two sandwich ELISAs using coated anti-VEGF Ab and soluble Flt-1 receptor fusion protein (sFlt-1/Fc). As low as 200 pg/ml of hVEGF diluted in human sera was detectable by these assays. In addition, we found that VEGF inhibitors ($2{\mu}g/ml$ of either anti-VEGF Ab or sFlt-1/Fc) completely block 5 ng/ml VEGF in these ELISAs. Subsequently, two bioassays, wound healing and HUVEC tube formation assays, revealed that anti-VEGF Ab $(1{\mu}g/ml)$ & sFlt-1/Fc Ab $(1{\mu}g/ml)$, or SU5416 (VEGFR tyrosine kinase inhibitor, $1{\mu}M$) prevents the activity of VEGF $(1{\sim}10ng/ml)$. Finally, secretion of MMP-9 by VEGF-stimulated macrophages was abolished by treatment of anti-VEGF Ab $(1{\mu}g/ml)$ in gelatin zymography. Conclusion: ELISAs together with bioassays developed in this study are appropriate for evaluation of the efficacy of inhibitors of VEGF.

• Journal of Information Processing Systems
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• 제15권3호
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• pp.604-615
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• 2019

Single-user spectrum sensing is susceptible to multipath effects, shadow effects, hidden terminals and other unfavorable factors, leading to misjudgment of perceived results. In order to increase the detection accuracy and reduce spectrum sensing cost, we propose an adaptive cooperative sensing strategy based on an estimated signal-to-noise ratio (SNR). Which can adaptive select different sensing strategy during the local sensing phase. When the estimated SNR is higher than the selection threshold, adaptive double threshold energy detector (ED) is implemented, otherwise cyclostationary feature detector is performed. Due to the fact that only a better sensing strategy is implemented in a period, the detection accuracy is improved under the condition of low SNR with low complexity. The local sensing node transmits the perceived results through the control channel to the fusion center (FC), and uses voting rule to make the hard decision. Thus the transmission bandwidth is effectively saved. Simulation results show that the proposed scheme can effectively improve the system detection probability, shorten the average sensing time, and has better robustness without largely increasing the costs of sensing system.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2022년도 춘계학술발표대회
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• pp.641-642
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• 2022

단일 데이터로부터의 이동 객체에 대한 행동 인식 연구는 데이터 수집 과정에서 발생하는 노이즈의 영향을 크게 받는다. 본 논문은 영상 데이터와 센서 데이터를 이용하여 다중 융합 네트워크 기반 이동 객체 행동 인식 방법을 제안한다. 영상으로부터 객체가 감지된 영역의 추출과 센서 데이터의 이상치 제거 및 결측치 보간을 통해 전처리된 데이터들을 융합하여 시퀀스를 생성한다. 생성된 시퀀스는 CNN(Convolutional Neural Networks)과 LSTM(Long Short Term Memory)기반 다중 융합 네트워크 모델을 통해 시계열에 따른 행동 특징들을 추출하고, 깊은 FC(Fully Connected) 계층을 통해 특징들을 융합하여 행동을 예측한다. 본 연구에서 제시된 방법은 사람을 포함한 동물, 로봇 등의 다양한 객체에 적용될 수 있다.

• 마이크로전자및패키징학회지
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• 제17권4호
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• pp.49-60
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• 2010

최근 휴대폰, PDA 등과 같은 모바일 전자 기기들의 사용이 급증하면서 다기능, 고성능, 초소형의 패키지가 시장에서 요구되고 있다. 따라서 사용되는 패키지의 크기도 더 작아지고 얇아지고 있다. 패키지에 사용되는 실리콘 다이 및 기판의 두께가 점점 얇아지면서 휨 변형, 크랙 발생, 및 기타 여러 신뢰성 문제가 크게 대두되고 있다. 이러한 신뢰성 문제는 서로 다른 패키지 재료의 열팽창계수의 차이에 의하여 발생된다. 따라서 초박형의 패키지의 경우 적절한 패키지물질과 두께 및 크기 등의 선택이 매우 중요하다. 본 논문에서는 현재 모바일 기기에 주로 사용되고 있는 CABGA, fcSCP, SCSP 및 MCP (Multi-Chip Package) 패키지에 대하여 휨과 응력의 특성을 수치해석을 통하여 연구하였다. 특히 휨 현상에 영향을 줄 수 있는 여러 중요 인자들, 즉 EMC 몰드의 두께 및 물성(탄성계수 및 열팽창 계수), 실리콘 다이의 두께와 크기, 기판의 물성 등이 휨 현상에 미치는 영향을 전반적으로 고찰하였다. 이를 통하여 휨 현상 메커니즘과 이를 제어하기 위한 중요 인자를 이해함으로써 휨 현상을 최소화 하고자 하였다. 휨 해석 결과 가장 큰 휨 값을 보인 SCSP에 대하여 실험계획법의 반응표면법을 이용하여 휨이 최소화되는 최적 조합을 구하였다. SCSP 패키지에서 휨에 가장 큰 영향을 미치는 인자는 EMC 두께 및 열팽창 계수, 기판의 열팽창계수, 그리고 실리콘 다이의 두께였다. 궁극적으로 최적화 해석을 통하여 SCSP의 휨을 $10{\mu}m$로 줄일 수 있음을 알 수 있었다.

• IMMUNE NETWORK
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• 제8권1호
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• pp.21-28
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• 2008

Background: A co-inhibitory molecule, B7-H4, is believed to negatively regulate T cell immunity by suppressing T cell proliferation and inhibiting cytokine production. However, the mechanism behind B7-H4-mediated tolerance remains unclear. Methods: Balb/c $(H-2^d)$ mice were fed with dendritic cell line, DC2.4 $(H-2^d)$ every day for 10 days. Meantime, mice were hydrodynamically injected with recombinant plasmid expressing B7-H4 fusion protein (B7-H4.hFc) or hFc via tail vein. One day after last feeding, mice were immunized with allogeneic B6 spleen cells. 14 days following immunization, mice were challenged with B6 spleen cells to ear back and the ear swelling was determined the next day. Subsequently, a mixed lymphocyte reaction (MLR) was also performed and cytokines profiles from the reaction were examined by sandwich ELISA. Frequency of immunosuppressive cell population was assayed with flow cytometry and mRNA for FoxP3 was determined by RT-PCR. Results: Tolerant mice given plasmid expressing B7-H4.hFc showed a significant reduction in ear swelling compared to control mice. In addition, T cells from mice given B7-H4.hFc plasmid revealed a significant hyporesponsiveness of T cells against allogeneic spleen cells and showed a significant decrease in Th1 and Th2 cytokines such as IFN-${\gamma}$, IL-5, and TNF-${\alpha}$. Interestingly, flow cytometric analysis showed that the frequency of CD4+CD25+FoxP3+ Tregs in spleen was increased in tolerant mice given recombinant B7-H4.hFc plasmid compared to control group. Conclusion: Our results demonstrate that B7-H4 synergistically potentiates oral tolerance induced by allogeneic cells by increasing the frequency of FoxP3+ CD4+CD25+ Treg and reducing Th1 and Th2 cytokine production.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제9권9호
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• pp.3312-3334
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• 2015

In this paper, a mechanism for spectrum allocation in overlay cognitive radio networks is proposed. In overlay cognitive radio networks, the secondary users (SUs) must first sense the activity of primary users (PUs) to identify unoccupied spectrum bands. Based on their different contributions for the spectrum sensing, the SUs get payoffs that are computed by the fusion center (FC). The unoccupied bands will be auctioned and SUs are asked to bid using payoffs they earned or saved. Coalitions are allowed to form among SUs because each SU may only need a portion of the bands. We formulate the coalition forming process as a coalition forming game and analyze it by game theory. In the coalition formation game, debtor-creditor relationship may occur among the SUs because of their limited payoff storage. A debtor asks a creditor for payoff help, and in return provides the creditor with a portion of transmission time to relay data for the creditor. The negotiations between debtors and creditors can be modeled as a Bayesian game because they lack complete information of each other, and the equilibria of the game is investigated. Theoretical analysis and numerical results show that the proposed auction yields data rate improvement and certain fairness among all SUs.

• Molecules and Cells
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• 제26권3호
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• pp.270-277
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• 2008

This study addresses the physiological functions of the Ran-binding protein homolog NbRanBP1 in Nicotiana benthamiana. Virus-induced gene silencing (VIGS) of NbRanBP1 caused stunted growth, leaf yellowing, and abnormal leaf morphology. The NbRanBP1 gene was constitutively expressed in diverse tissues and an NbRanBP1:GFP fusion protein was primarily localized to the nuclear rim and the cytosol. BiFC analysis revealed in vivo interaction between NbRanBP1 and NbRan1 in the nuclear envelope and the cytosol. Depletion of NbRanBP1 or NbRan1 reduced nuclear accumulation of a NbBTF3:GFP marker protein. In the later stages of development, NbRanBP1 VIGS plants showed stress responses such as reduced mitochondrial membrane potential, excessive production of reactive oxygen species, and induction of defense-related genes. The molecular role of RanBP1 in plants is discussed in comparison with RanBP1 function in yeast and mammals.