• Title/Summary/Keyword: Fast separation

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Gas Separation Properties and Their Applications of High Permeable Amorphous Perfluoropolymer Membranes (고투과성 무정형 불소고분자 불리막의 기체분리 특성 및 응용)

  • Freeman, Benny D.;Park, Ho-Bum
    • Membrane Journal
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    • v.17 no.2
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    • pp.81-92
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    • 2007
  • Membrane-based separation processes are receiving increasing attention in the scientific community and industry since they provide a desirable alternative to processes that are not easy to achieve by conventional separation technologies. In particular, gas separation using polymeric membranes have annually grown so fast owing to advantages such as easy installation, no moving parts, small footprint and low energy process. The key element is definitely a polymer membrane exhibiting high permeability and high selectivity to compete with other gas separation technologies. Current polymer membranes used for commercial gas separation are a family of hydrocarbon polymers for hydrogen separation, air separation and carbon dioxide separation from natural gas sweetening. Relatively, gas or vapor separation properties of fluoropolymers are not known so much as compared with those of hydrocarbon polymers. Accordingly, in this study, membranes prepared from amorphous perfluoropolymers are of particular interest because of the unique properties of these polymers. The advantages offered by these amorphous perfluoropolymers for use in gas and vapor separation will be discussed. In addition, membrane properties and separation performance will be compared with other membranes available on the market.

Comparison of independent component analysis algorithms for low-frequency interference of passive line array sonars (수동 선배열 소나의 저주파 간섭 신호에 대한 독립성분분석 알고리즘 비교)

  • Kim, Juho;Ashraf, Hina;Lee, Chong-Hyun;Cheong, Myoung Jun
    • The Journal of the Acoustical Society of Korea
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    • v.38 no.2
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    • pp.177-183
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    • 2019
  • In this paper, we proposed an application method of ICA (Independent Component Analysis) to passive line array sonar to separate interferences from target signals in low frequency band and compared performance of three conventional ICA algorithms. Since the low frequency signals are received through larger bearing angles than other frequency bands, neighboring beam signals can be used to perform ICA as measurement signals of the ICA. We use three ICA algorithms such as Fast ICA, NNMF (Non-negative Matrix Factorization) and JADE (Joint Approximation Diagonalization of Eigen-matrices). Through experiments on real data obtained from passive line array sonar, it is verified that the interference can be separable from target signals by the suggested method and the JADE algorithm shows the best separation performance among the three algorithms.

Fast Microchip Electrophoresis Using Field Strength Gradients for Single Nucleotide Polymorphism Identification of Cattle Breeds

  • Oh, Doo-Ri;Cheong, Il-Cheong;Lee, Hee-Gu;Eo, Seong-Kug;Kang, Seong-Ho
    • Bulletin of the Korean Chemical Society
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    • v.31 no.7
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    • pp.1902-1906
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    • 2010
  • A microchip electrophoresis (ME) method was developed using a programmed field strength gradients (PFSG) for the single nucleotide polymorphism (SNP) based fast identification of cattle breeds. Four different Korean cattle (Hanwoo) and Holstein SNP markers amplified by allele-specific polymerase chain reaction were separated in a glass microchip filled with 0.5% poly(ethyleneoxide) ($M_r$ = 8 000 000) by PFSG as follows: 750 V/cm for 0 - 14 s, 166.7 V/cm for 14 - 31 s, 83.3 V/cm for 31 - 46 s, and 750 V/cm for 46 - 100 s. The cattle breeds were clearly distinguished within 45 s. The ME-PFSG method was 7 times and 5 times faster than the constant electric field ME method and the capillary electrophoresis- PFSG method, respectively, with a high resolving power ($R_s$ = 5.05 - 9.98). The proposed methodology could be a powerful tool for the fast and simultaneous determination of SNP markers for various cattle breeds with high accuracy.

Affinity chromatography and capillary electrophoresis for analysis of the yeast ribosomal proteins

  • Goyder, Miriam S.;Willison, Keith R.;Klug, David R.;DeMello, Andrew J.;Ces, Oscar
    • BMB Reports
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    • v.45 no.4
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    • pp.233-238
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    • 2012
  • We present a top down separation platform for yeast ribosomal proteins using affinity chromatography and capillary electrophoresis which is designed to allow deposition of proteins onto a substrate. FLAG tagged ribosomes were affinity purified, and rRNA acid precipitation was performed on the ribosomes followed by capillary electrophoresis to separate the ribosomal proteins. Over 26 peaks were detected with excellent reproducibility (<0.5% RSD migration time). This is the first reported separation of eukaryotic ribosomal proteins using capillary electrophoresis. The two stages in this workflow, affinity chromatography and capillary electrophoresis, share the advantages that they are fast, flexible and have small sample requirements in comparison to more commonly used techniques. This method is a remarkably quick route from cell to separation that has the potential to be coupled to high throughput readout platforms for studies of the ribosomal proteome.

Controlling the pore size of macroporous membranes by adding non-solvent

  • Shin, Se-Jong;Im, Se-Jun;Park, Seung-Ryul;Lee, Seung-Yun;Min, Byoung-Ryul
    • Proceedings of the Membrane Society of Korea Conference
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    • 2003.07a
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    • pp.107-114
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    • 2003
  • This study investigated the effect of 2-methoxy ethanol (2-Me) non-solvent as additive included in casting solution. Macroporous polymer membranes were prepared by using polyethersufone (PES)/N-methyl-2-pyrrolidone (NMP)/2-Me casting solution and water coagulant. The phase separation co-process of the vapor-induced phase separation (VIPS) and liquid-induced phase separation (LIPS) were used by means of membrane preparation method. The pore size and pore size distribution were controlled with additive (non-solvent), and measured with Automated Perm Porometer. By increasing additive (non-solvent) in the casting solution, the membranes produced changed from finger structure to sponge structure. That is due to the different diffusion rates. At slow diffusion process, sponge-like structure was formed and at fast diffusion process, finger-like structure was formed. Also relative humidity, evaporation time, temperature of casting solution and coagulation bath etc. had effects on the pore size distribution and the porosity of the membrane.

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Separation-hybrid models for simulating nonstationary stochastic turbulent wind fields

  • Long Yan;Zhangjun Liu;Xinxin Ruan;Bohang Xu
    • Wind and Structures
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    • v.38 no.1
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    • pp.1-13
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    • 2024
  • In order to effectively simulate nonstationary stochastic turbulent wind fields, four separation hybrid (SEP-H) models are proposed in the present study. Based on the assumption that the lateral turbulence component at one single-point is uncorrelated with the longitudinal and vertical turbulence components, the fluctuating wind is separated into 2nV-1D and nV1D nonstationary stochastic vector processes. The first process can be expressed as double proper orthogonal decomposition (DPOD) or proper orthogonal decomposition and spectral representation method (POD-SRM), and the second process can be expressed as POD or SRM. On this basis, four SEP-H models of nonstationary stochastic turbulent wind fields are developed. In addition, the orthogonal random variables in the SEP-H models are presented as random orthogonal functions of elementary random variables. Meanwhile, the number theoretical method (NTM) is conveniently adopted to select representative points set of the elementary random variables. The POD-FFT (Fast Fourier transform) technique is introduced in frequency to give full play to the computational efficiency of the SEP-H models. Finally, taking a long-span bridge as the engineering background, the SEP-H models are compared with the dimension-reduction DPOD (DR-DPOD) model to verify the effectiveness and superiority of the proposed models.

A Sequential Joint Maximum Likelihood Algorithm for Blind Co-Channel Signal Separation (블라인드 동채널 신호 분리를 위한 순차적인 Joint Maximum Likelihood 알고리듬)

  • Inseon Jang;Park, Seungjin
    • Proceedings of the IEEK Conference
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    • 2001.09a
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    • pp.85-88
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    • 2001
  • In this paper we consider a problem of blind co-channel signal separation, the goal of which is to estimate multiple co-channel digitally modulated signals using an antenna array. We employ the joint maximum likelihood estimation and present a sequential algorithm, which is referred to as sequential joint maximum likelihood (SJML) algorithm. It separates multiple co-channel signal on-line and converges fast in overdetermined noisy communication environment. And the computational complexity of SJML for M-QAM (M=8, 16, 64,...) signals is less expensive compared to the SLSP. Useful behavior of this algorithm are confirmed by simulations.

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Magnetic beads separation using a multi-layered microfluidic channel (다층구조의 미세유체채널을 이용한 자성입자 분리)

  • Lee, Hye-Lyn;Song, Suk-Heung;Jung, Hyo-Il
    • Proceedings of the KSME Conference
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    • 2008.11a
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    • pp.1685-1686
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    • 2008
  • This paper presents the design and experiment results of a multi-layered microsystem for magnetic bead applications. The magneto-microfluidic device is designed for capable of separating magnetic beads. In the presence of the magnetic field, magnetic beads are attracted and moved to high gradient magnetic fields. A multi-layered microfluidic channel consists of top and bottom layers in order to separate magnetic beads in the vertical direction. Our channel is easily integrated magnetic cell sorter, especially on-chip microelectromagnet or permanent magnet device. Fast separation of magnetic beads in top and bottom channels can be used in high throughput screening to monitor the efficiency of blood and drug compounds.

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The Phase Separation Phenomena of PSf/NMP Solutions in Varying Relative Humidities

  • 김윤보;박현채;강용수;김화용
    • Proceedings of the Membrane Society of Korea Conference
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    • 1995.10a
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    • pp.45-46
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    • 1995
  • Generally, two different techniques have been employed for the precipitation of membranes from a polymer casting solution. In the first method, a precipitant is introduced from a vapor phase. In this case the precipitaion rate is very slow and a more or less symmetric structure is formed. In the second method, a precipitant is added to a casting solution by immersing the cast polymer film in a nonsolvent bath. In this case the precipitation rate is very fast and a skinned asymmetric membrane structure is obtained. In this study, we introduced water-vapor to PSf/NMP solution and took photographs of phase separation phenomena of polymer lean phase.

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Separation and Determination of Cefatrizine ${\cdot}$ Propylene Glycol by High-performance Liquid Chromatography (High Performance Liquid Chromatography 를 이용한 Cefatrizine ${\cdot}$ Propylene Glycol 의 분리 및 정량)

  • Kwon, Shoon-Ja;Lee, Ki-Chang;Choe, Koang-Hoon
    • Journal of the Korean Applied Science and Technology
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    • v.6 no.1
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    • pp.27-30
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    • 1989
  • The fast separation and determination of cefatrizine${\cdot}$propylene glycol and inmpurities - TACA: 7-amino-3-(1,2,3-triazol-4-yl)thiomethyl-3-cephem-4-carboxylic acid and 7-ACA; 7-amino cephalosporanic acid - was performed by the high poerformance liquid chromatography using octadecyl siland (ODS) column. Methanol and ammonium phosphate buffer [$0.03M(NH_4)_2\;HPO_4$, (pH 7.5)] was used analyze, as eluent. The experimental value of the contents of cefatrizine${\cdot}$propylene glycol and impurities agree with the theoretical value of those.