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http://dx.doi.org/10.5012/bkcs.2010.31.7.1902

Fast Microchip Electrophoresis Using Field Strength Gradients for Single Nucleotide Polymorphism Identification of Cattle Breeds  

Oh, Doo-Ri (Department of Chemistry and Research Institute of Physics and Chemistry (RINPAC), Chonbuk National University)
Cheong, Il-Cheong (Hankyong National University)
Lee, Hee-Gu (Medical Genomic Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Eo, Seong-Kug (Department of Microbiology, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
Kang, Seong-Ho (Department of Chemistry and Research Institute of Physics and Chemistry (RINPAC), Chonbuk National University)
Publication Information
Bulletin of the Korean Chemical Society / v.31, no.7, 2010 , pp. 1902-1906 More about this Journal
Abstract
A microchip electrophoresis (ME) method was developed using a programmed field strength gradients (PFSG) for the single nucleotide polymorphism (SNP) based fast identification of cattle breeds. Four different Korean cattle (Hanwoo) and Holstein SNP markers amplified by allele-specific polymerase chain reaction were separated in a glass microchip filled with 0.5% poly(ethyleneoxide) ($M_r$ = 8 000 000) by PFSG as follows: 750 V/cm for 0 - 14 s, 166.7 V/cm for 14 - 31 s, 83.3 V/cm for 31 - 46 s, and 750 V/cm for 46 - 100 s. The cattle breeds were clearly distinguished within 45 s. The ME-PFSG method was 7 times and 5 times faster than the constant electric field ME method and the capillary electrophoresis- PFSG method, respectively, with a high resolving power ($R_s$ = 5.05 - 9.98). The proposed methodology could be a powerful tool for the fast and simultaneous determination of SNP markers for various cattle breeds with high accuracy.
Keywords
Cattle breed; Microchip gel electrophoresis; Field strength gradients; Fast separation; Single nucleotide polymorphism (SNP);
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
Times Cited By SCOPUS : 1
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