• 미생물학회지
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• 제29권5호
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• pp.319-328
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• 1991

this study was performed for the purpose of surveying the distribution and characteristics of the Vibrio species in the diseased farm flounders (Paralichthys olivaceus) during the winter season in Korea. Samples were collected along the southern coast in the Sinam, Keoje Island, and Teosu areas from November '90 to Feburary '91. Nine species of Vibrio were identified as V. tubiashii (85 strains), V. damsela (78 strains), V. anguillarum (21 strains), V. compbelli (9 strains), V. fluvialis (9 strain), V. costicola (1 strain), V. alginolyticus (1 strain), V. gazogenes (1 strain), V. marinus (1 strain), and unidentified 3 groups of bacteria (154 strains) which may have related with V. tubiashii were isolated. Eleven kinds of discs (BBL Co.) were used for antibiotic susceptibility test and 116 strains were selected for the experiment. Most bacteria were powerfully inhibited their growth by 4 antibiotics such as cephalothin, chloramphenicol, colistin, and tetracycline, but they were poorly inhibited by 4 antibiotics such as carbenicillin, kanamycin, penicillin G, and streptomycin.

• 한국수산과학회지
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• 제53권4호
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• pp.492-498
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• 2020

Despite its economic importance, olive flounder Paralichthys olivaceus aquaculture industry is facing a crisis with a continuous production decline. There have been many solutions to overcome the complicate predicament proposed. Increasing genetic diversity and discovering new commercial value through selective breeding are among them. The aims of the present study are to increase the selection power of the golden flounders. We examined the genetic diversity of the breeder population of golden flounders and developed selective markers for the golden flounder population. The 6 microsatellite (MS) markers were selected from melanogenesis-related genes, which are believed to be involved in the pigmentation of fish. All markers were polymorphic (except PO4) and 5 of them had PIC value of 0.6 or above. All makers had distinctive alleles indicating either normal or golden individuals. For examples, from PO4 marker, the frequency of an allele (316) in the golden population was 100% and in normal population was 0% (P<0.001). Although some more studies with more samples at the later generations should be performed to confirm this result, the 316 allele from PO4 marker could be a distinctive tool for decision of the colors in olive flounders at an early stage of the life cycle.

• 현장농수산연구지
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• 제23권1호
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• pp.81-87
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• 2021

2020년 11월, 국내의 넙치 양식장에서 양식 중이던 넙치가 이상 유영 소견을 보이다가, 지속적으로 폐사하였다. 질병 진단 과정 중, 폐사어의 신장에서 세균(KNCFKW-PN1)이 분리되었다. gyrase B subunit 유전자의 시퀀스 분석 결과, KNCFKW-PN1 분리주는 기존에 보고된 LMG 2227^T 균주의 해당 유전자 시퀀스와 99.59% 유사도를 보여 Pseudoalteromonas nigrifaciens 로 동정이 되었다. 항생제 감수성 실험 결과에 따르면, KNCFKW-PN1 분리주는 ciprofloxacin에 대하여 중등도의 내성을 나타내었고, ampicillin, cefepime, cefotaxime, ceftazidime, amikacin에 내성을 나타내었다. 본 사례는 다제 내성 Pseudoalteromonas nigrifaciens 세균이 넙치로부터 분리된 최고의 보고이다.

• 한국어병학회지
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• 제37권1호
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• pp.71-78
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• 2024

Streptococcosis, caused by Streptococcus iniae and Streptococcus parauberis is an important bacterial disease that affects in olive flounder in Korea. In Korea, multivalent bacterial vaccines are used to prevent streptococcal diseases in aquaculture. In this study, commercial vaccines containing formalin-inactivated bacterial cells of S. iniae and S. parauberis were administered at six fish farms and one unvaccinated fish farm were designated for investigation (Wando; 4 sites and Jeju; 3 sites). Blood was collected from vaccinated and unvaccinated olive flounders, and titers of antibodies against S. iniae and S. parauberis in serum were analyzed using ELISA. After a one shot vaccination in the farms at Jeju (farm A) and Wando (farm D), the proportion of individuals with specific antibodies against S. parauberis OD values of 0.4 or higher was 60% and 53.5%, respectively. But after booster vaccination, the proportion of individuals with serum OD values of 0.4 or higher was higher substantially increased to 96.6% (farm A) and 100% (farm D). The levels of S. parauberis specific antibodies of olive flounder were increased after vaccination in three fish farms (farm D, E, and F), but not S. iniae specific antibodies.

• 환경생물
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• 제19권2호
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• pp.173-181
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• 2001

제주도내 넙치양어장에 빈발하여 피해를 입히는 에드와드병의 원인균인 Edwardsiella tarda의 출현 시기와 분포를 알기 위하여, 1997년 6월부터 1998년 5월가지 제주도내 5개 넙치양어장에서 사용하고 있는 해수(주입수, 사육수, 배출수)와 사육중인 넙치의 장기조직 (간장, 비장, 신장, 장)을 조사하였다. 조사 방법은 해수인 경우는 DSSS배지에 직접 도말하는 방법과, 증균 배양후 도말하는 방법으로 균수를 계수하였고, 장기 조직은 각 장기를 호모게나이져 한 후 10배 희석계열을 만들어 DSSS한천배지에 도말하고 균수를 계수하였다. 그 결과를 요약하면 5개 넙치양어장의 해수에서 E. tarda의 검출은 주입수에서는 1997년 7월, 9월, 11월과 1998년 2월, 3월, 4월에 검출되었고, 사육수 및 배출수에서는 연중 검출되었으며, 특히 고수온기인 6월에서 9월 사이에 다른 달에 비하여 검출량이 높게 나타났다. 양어장에 따른 장기조직 내에서 E. tarda의 분포는 순환율이 낮고, 배설물과 찌꺼기 배출이 잘 되지 않은 장타원형 수조인 B양어장에서 검출량이 높게 나타났으며, 순환율이 높고 유기물 배출이 잘되는 A양어장은 전혀 검출이 안 되었으며, 동일조건인 양어장에서는 낮게 나타났다. 넙치 장기조직에 따른 E. tarda의 검출은 간장과 장에서 대체로 검출량이 높게 나타났으며, 특히 고수온기인 6월과 9월 사이에 높게 검출되었다.

• 대한수의학회지
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• 제47권3호
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• pp.291-298
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• 2007

Olive flounder, Paralichthys olivaceus is one of the most important cultured fish in Korea, its farming has been negatively impacted by viral, bacterial and parasitic diseases. Streptococcal infection was considered as a serious problem because of significant economic losses in olive flounder farm industry. The development and evaluation of vaccine for protection against infection by this agent were required. We evaluated the safety and efficacy of ${\beta}$-hemolytic Streptococcus (S.) iniae vaccine on olive flounder Three hundreds of flounders (weight $119.8{\pm}20.7g$, body length $22.6{\pm}1.4cm$) were reared in 0.5 tons aquaria in land-marine tank system. Seawater was provided from the sea of Inchon in Korea, and water temperature was set to $22^{\circ}C$ and $25^{\circ}C$ in the vaccination and challenge test, respectively. We used the formalin-inactivated ${\beta}$-hemolytic S. iniae (F2K) vaccine (M VAC INIAE; Kyoritsu seiyaku, Japan) originated in Japan. The vaccine was intraperitoneally administered to fish. Both of vaccinated group and control group were challenged with intraperitoneally injection by virulent S. iniae SI-36 isolates with $1.0{\times}10^7CFU/fish$ at 3 weeks after vaccination. Difference on mortality of control and vaccinated group (90.0 and 15.0%, 76.5 and 8.0% respectively) at two trials were found significant (p<0.05), and relative percent survival were 83.4% and 89.5%, respectively. The dead fishes were showed dark pigmentation of skin, abdominal extension, hemorrhagic ascites, and liver necrosis, and isolated the S. iniae strain from ascites, liver and kidney. We confirmed the safety and efficacy of ${\beta}$-hemolytic S. iniae vaccine by determinations of the optimal management condition and artificial challenge test in olive flounder.

• 한국임상수의학회지
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• 제26권3호
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• pp.226-230
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• 2009

We evaluated the efficacy of $\beta$-hemolytic Streptococcus(S.) iniae vaccine on cultured olive flounder. Three hundred flounders(weight $50{\pm}5$ g) were obtained from two farm at Wando and Taean in the southern and western coast of Korea at May and June 2007, respectively. Twenty of flounders moved in 0.5 tons aquaria in land-marine tank system of National Veterinary Research and Quarantine Service. Seawater was transported from the sea of Inchon in western Korea, and water temperature maintained to $22^{\circ}C$ and $25^{\circ}C$ during the vaccination and challenge test, respectively. We used the formalin-inactivated $\beta$-hemolytic S. iniae vaccine produced by domestic manufacturers. The vaccine was intraperitoneally administered to fish. The vaccinated and control group were challenged with intraperitoneal injection by virulent S. iniae SI-36 isolates with $5.0{\times}10^8$ CFU/fish at 3 weeks after vaccination. We evaluated the vaccine efficacy by calculating numbers of dead fish, and observing of clinical signs, exterior and gross lesions, and examining bacteria isolation and identification. Thirty-four(25.2%) of 135 control and vaccinated group fish were dead with serious anemia, abdominal extension, and hernia of intestine during 3 weeks post vaccination. We isolated Neoheterobothrium hirame from the buccal cavity and Edwardsiella tarda from kidney of dead and diseased fish. When infected fish with these agents were challenged with S. iniae SI-36 isolates, the cumulative mortality of control and vaccinated group were 86.7, and 46.7%, respectively. However, significant differences(p<0.05) were observed on cumulative mortality between control(20.0%) and vaccinated group(95.0%) at second trials with 40 healthy, and relative percent survival(RPS) was 78.0%. We confirmed that the efficacy of $\beta$-hemolytic S. iniae vaccine on olive flounder were impacted by health condition such as bacterial and parasitic diseases.