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http://dx.doi.org/10.5657/KFAS.2020.0492

Identification of Genetic Markers Distinguishing Golden Flounders from Normal Olive Flounders Paralichthys olivaceus Using Microsatellite Markers  

Kim, Min Sung (BLUGEN KOREA)
Kwak, Ju Ri (BLUGEN KOREA)
Kim, Tae Hwan (BLUGEN KOREA)
Han, Jae Yong (BLUGEN KOREA)
Park, Ji Been (BLUGEN KOREA)
Jo, Hyeon Kyeong (BLUGEN KOREA)
Suh, Jong-pyo (Haeyeon Fish Farm)
Lee, Woo-jai (BLUGEN KOREA)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.53, no.4, 2020 , pp. 492-498 More about this Journal
Abstract
Despite its economic importance, olive flounder Paralichthys olivaceus aquaculture industry is facing a crisis with a continuous production decline. There have been many solutions to overcome the complicate predicament proposed. Increasing genetic diversity and discovering new commercial value through selective breeding are among them. The aims of the present study are to increase the selection power of the golden flounders. We examined the genetic diversity of the breeder population of golden flounders and developed selective markers for the golden flounder population. The 6 microsatellite (MS) markers were selected from melanogenesis-related genes, which are believed to be involved in the pigmentation of fish. All markers were polymorphic (except PO4) and 5 of them had PIC value of 0.6 or above. All makers had distinctive alleles indicating either normal or golden individuals. For examples, from PO4 marker, the frequency of an allele (316) in the golden population was 100% and in normal population was 0% (P<0.001). Although some more studies with more samples at the later generations should be performed to confirm this result, the 316 allele from PO4 marker could be a distinctive tool for decision of the colors in olive flounders at an early stage of the life cycle.
Keywords
Olive flounder; Leucism; Microsatellite; Genetic diversity;
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