Journal of Practical Agriculture & Fisheries Research (현장농수산연구지)

Korea National University of Agriculture and Fisheries (국립한국농수산대학교 교육개발센터)
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Isolation and Identification from Korean Olive Flounders (Paralichthys olivaceus) Showing Abnormal Swimming Behavior, and Sstudy of Antibiotic Susceptibility

이상유영 증상 국내산 양식 넙치에서의 슈도알테로모나스 분리·동정 및 항생제 감수성 연구

  • Kwon, H. (Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Yang, S. (Department of Aquaculture, Korea National College of Agriculture and Fisheries) ;
  • Kim, J.H. (Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jun, J.W. (Department of Aquaculture, Korea National College of Agriculture and Fisheries)
  • 권혜민 (한국생명공학연구원 감염병연구센터) ;
  • 양소리 (한국농수산대학 어류양식학과) ;
  • 김지형 (한국생명공학연구원 감염병연구센터) ;
  • 전진우 (한국농수산대학 어류양식학과)
  • Received : 2021.05.04
  • Accepted : 2021.06.14
  • Published : 2021.06.28
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Abstract

Olive flounders which were cultured in commercial fish farm showed abnormal swimming behavior in November 2020. Then, gradual mortality was observed in the fish farm. During the diagnosis, bacterial strain KNCFKW-PN1 was isolated from the kidney of the dead fish. Based on the sequence of gyrase B subunit gene, KNCFKW-PN1 was proved to be Pseudoalteromonas nigrifaciens, showing 99.59% nucleotide identity with that of P. nigrifaciens LMG 2227T. According to the result of antibiotic susceptibility test, P. nigrifaciens KNCFKW-PN1 showed intermediate resistance to ciprofloxacin, and was resistant to ampicillin, cefepime, cefotaxime, ceftazidime, and amikacin. This is the first report of the isolation of multiple-antibiotic-resistant P. nigrifaciens from olive flounder.

2020년 11월, 국내의 넙치 양식장에서 양식 중이던 넙치가 이상 유영 소견을 보이다가, 지속적으로 폐사하였다. 질병 진단 과정 중, 폐사어의 신장에서 세균(KNCFKW-PN1)이 분리되었다. gyrase B subunit 유전자의 시퀀스 분석 결과, KNCFKW-PN1 분리주는 기존에 보고된 LMG 2227T 균주의 해당 유전자 시퀀스와 99.59% 유사도를 보여 Pseudoalteromonas nigrifaciens 로 동정이 되었다. 항생제 감수성 실험 결과에 따르면, KNCFKW-PN1 분리주는 ciprofloxacin에 대하여 중등도의 내성을 나타내었고, ampicillin, cefepime, cefotaxime, ceftazidime, amikacin에 내성을 나타내었다. 본 사례는 다제 내성 Pseudoalteromonas nigrifaciens 세균이 넙치로부터 분리된 최고의 보고이다.

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