• Applied Chemistry for Engineering
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• v.20 no.5
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• pp.561-564
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• 2009

Filter media finely interspersed with activated carbons were prepared by a needle punching process without using chemical binders. Their characteristics were investigated efficiently to abate environmentally harmful gas such as acetaldehyde, and were compared with those of cabin air filter coated with activated carbons by using chemical binders. These combination filters were installed on a vehicle fan placed in a test chamber of capacity similar to the interior volume of a commercially available passenger car, and the efficiency of acetaldehyde abatement was measured as a function of time. The filter utilizing chemical binders showed somewhat better performance for the elimination of acetaldehyde despite the adverse effect of the chemical binder that would clog the micropores of the activated carbons. It turned out that the needle punching process had the activated carbons agglomerated due to hydrophobic interactions, resulting in a relatively larger void area than that of the filter utilizing chemical binders.

• Journal of the Korean Society of Hazard Mitigation
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• v.8 no.3
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• pp.37-42
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• 2008

This study is focused on the numerical predictions for different smoke-control ventilation modes for the subway fire. Smoke-control ventilation mode in the domestic platform is that exhausting for the smoke detected zones while supplying air or stopping any ventilation for other zones in the platform. Three cases of smoke control ventilation modes are considered. First and second case are present running smoke control mode in Korea. The third is that smoke-control ventilation fans equipped in the platform are operated in first 4 minute(platform evacuation time, NFPA130) since then the fans equipped in the platform are stopped and the fans equipped in the tunnels are operated. Distributions of heat, carbon monoxide and visible range are compared at a height of 1.7m(passenger breath/sight height) above the platform. The numerical results show that air supply fan operation in the platform causes the smoke disturbance and a rapid spread of the smoke. The switch-operation with fans in both the platform and tunnel are better than operation with only platform fans in smoke rejection efficiency.

• Proceedings of the Korean Institute Of Construction Engineering and Management
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• 2008.11a
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• pp.877-882
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• 2008

The column gap of underground parking lots of office considering a business background and effective parking divisions is planned. A parking plan by column gap of fixed forms in atypical underground parking lots makes much extra storage rooms. Most of the edge of the parking lots are used for fan room. Part of the edge are located at parking ramps. Also warehouses are located in extra storage rooms. In this study compare study economical efficiency between life cycle costs and leases earnings. Consequently, It is proved that the warehouse rental is efficient because of lease earning guaranted years.

• Journal of Microbiology and Biotechnology
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• v.26 no.11
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• pp.1933-1942
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• 2016

A novel ${\alpha}-glucoside$ hydrolase (named PspAG97A) from glycoside hydrolase family 97 (GH97) was cloned from the deep-sea bacterium Pseudoalteromonas sp. K8, which was screened from the sediment of Kongsfjorden. Sequence analysis showed that PspAG97A belonged to GH97, and shared 41% sequence identity with the characterized ${\alpha}-glucoside$ BtGH97a. PspAG97A possessed three key catalytically related glutamate residues. Mutation of the glutamate residues indicated that PspAG97A belonged to the inverting subfamily of GH97. PspAG97A showed significant reversibility against changes in salt concentration. It exhibited halophilic ability and improved thermostability in NaCl solution, with maximal activity at 1.0 M NaCl/KCl, and retained more than 80% activity at NaCl concentrations ranging from 0.8 to 2.0 M for over 50 h. Furthermore, PspAG97A hydrolyzed not only ${\alpha}-1,4-glucosidic$ linkage, but also ${\alpha}-1,6-$ and ${\alpha}-1,2-glucosidic$ linkages. Interestingly, PspAG97A possessed high catalytic efficiency for long-chain substrates with ${\alpha}-1,6-linkage$. These characteristics are clearly different from other known ${\alpha}-glucoside$ hydrolases in GH97, implying that PspAG97A is a unique ${\alpha}-glucoside$ hydrolase of GH97.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2010.11a
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• pp.101-104
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• 2010

Recently, needs for UAVs and small aircraft and small turbo jet or turbo fan engines for these air-crafts are increasing. Size and weight are the two main restrictions in small air-crafts such as UAV or VLJ propulsion system applications. Therefore, high power density is required in small size and designers come up with unconventional solutions in the design of small aero gas turbine engines. One of the solutions is the usage of highly loaded axial compressors. This paper introduces an aerodynamic design method of a highly loaded axial compressor and its review process. Numerical simulation has been carried out to assess the aerodynamic performance of the compressor.

• Advances in aircraft and spacecraft science
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• v.3 no.3
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• pp.243-257
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• 2016

The increase of air traffic volume has brought an increasing amount of issues related to carbon and NOx emissions and noise pollution. Aircraft manufacturers are concentrating their efforts to develop technologies to increase aircraft efficiency and consequently to reduce pollutant discharge and noise emission. Ultra High By-Pass Ratio engine concepts provide reduction of fuel consumption and noise emission thanks to a decrease of the jet velocity exhausting from the engine nozzles. In order to keep same thrust, mass flow and therefore section of fan/nacelle diameter should be increased to compensate velocity reduction. Such feature will lead to close-coupled architectures for engine installation under the wing. A strong jet-wing interaction resulting in a change of turbulent mixing in the aeroacoustic field as well as noise enhancement due to reflection phenomena are therefore expected. On the other hand, pressure fluctuations on the wing as well as on the fuselage represent the forcing loads, which stress panels causing vibrations. Some of these vibrations are re-emitted in the aeroacoustic field as vibration noise, some of them are transmitted in the cockpit as interior noise. In the present work, the interaction between a jet and wing or fuselage is reproduced by a flat surface tangential to an incompressible jet at different radial distances from the nozzle axis. The change in the aerodynamic field due to the presence of the rigid plate was studied by hot wire anemometric measurements, which provided a characterization of mean and fluctuating velocity fields in the jet plume. Pressure fluctuations acting on the flat plate were studied by cavity-mounted microphones which provided point-wise measurements in stream-wise and spanwise directions. Statistical description of velocity and wall pressure fields are determined in terms of Fourier-domain quantities. Scaling laws for pressure auto-spectra and coherence functions are also presented.

• Journal of Microbiology and Biotechnology
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• v.31 no.1
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• pp.8-15
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• 2021

More and more available fungal genome sequence data reveal a large amount of secondary metabolite (SM) biosynthetic 'dark matter' to be discovered. Heterogeneous expression is one of the most effective approaches to exploit these novel natural products, but it is limited by having to clone entire biosynthetic gene clusters (BGCs) without errors. So far, few effective technologies have been developed to manipulate the specific large DNA fragments in filamentous fungi. Here, we developed a fungal BGC-capturing system based on CRISPR/Cas9 cleavage in vitro. In our system, Cas9 protein was purified and CRISPR guide sequences in combination with in vivo yeast assembly were rationally designed. Using targeted cleavages of plasmid DNAs with linear (8.5 kb) or circular (8.5 kb and 28 kb) states, we were able to cleave the plasmids precisely, demonstrating the high efficiency of this system. Furthermore, we successfully captured the entire Nrc gene cluster from the genomic DNA of Neosartorya fischeri. Our results provide an easy and efficient approach to manipulate fungal genomic DNA based on the in vitro application of Cas9 endonuclease. Our methodology will lay a foundation for capturing entire groups of BGCs in filamentous fungi and accelerate fungal SMs mining.

• The Korean Journal of Physiology and Pharmacology
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• v.25 no.2
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• pp.97-109
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• 2021

Neonatal hypoxia/ischemia (H/I), injures white matter, results in neuronal loss, disturbs myelin formation, and neural network development. Neuroinflammation and oxidative stress have been reported in neonatal hypoxic brain injuries. We investigated whether Paeoniflorin treatment reduced H/I-induced inflammation and oxidative stress and improved white matter integrity in a neonatal rodent model. Seven-day old Sprague-Dawley pups were exposed to H/I. Paeoniflorin (6.25, 12.5, or 25 mg/kg body weight) was administered every day via oral gavage from postpartum day 3 (P3) to P14, and an hour before induction of H/I. Pups were sacrificed 24 h (P8) and 72 h (P10) following H/I. Paeoniflorin reduced the apoptosis of neurons and attenuated cerebral infarct volume. Elevated expression of cleaved caspase-3 and Bad were regulated. Paeoniflorin decreased oxidative stress by lowering levels of malondialdehyde and reactive oxygen species generation and while, and it enhanced glutathione content. Microglial activation and the TLR4/NF-κB signaling were significantly down-regulated. The degree of inflammatory mediators (interleukin 1β and tumor necrosis factor-α) were reduced. Paeoniflorin markedly prevented white matter injury via improving expression of myelin binding protein and increasing O1-positive olidgodendrocyte and O4-positive oligodendrocyte counts. The present investigation demonstrates the potent protective efficiency of paeoniflorin supplementation against H/I-induced brain injury by effectually preventing neuronal loss, microglial activation, and white matter injury via reducing oxidative stress and inflammatory pathways.

• Korean Journal of Materials Research
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• v.31 no.12
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• pp.657-664
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• 2021

To build a highly active photocatalytic system with high efficiency and low cast of TiO₂, we report a facile hydrothermal technique to synthesize Ag₂Se-nanoparticle-modified TiO₂ composites. The physical characteristics of these samples are analyzed by X-ray diffraction, scanning electron microscopy with energy dispersive X-ray analysis, transmission electron microscopy and BET analysis. The XRD and TEM results show us that TiO₂ is coupled with small sized Ag₂Se nanoplate, which has an average grain size of about 30 nm in diameter. The agglomeration of Ag₂Se nanoparticles is improved by the hydrothermal process, with dispersion improvement of the Ag₂Se@TiO₂ nanocomposite. Texbrite BA-L is selected as a simulated dye to study the photodegradation behavior of as-prepared samples under visible light radiation. A significant enhancement of about two times the photodegradation rate is observed for the Ag₂Se@TiO₂ nanocomposite compared with the control sample P25 and as-prepared TiO₂. Long-term stability of Ag₂Se@TiO₂ is observed via ten iterations of recycling experiments under visible light irradiation.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2004.03a
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• pp.369-375
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• 2004

Gas turbine engine simulation in terms of transient, steady state performance and operational characteristics is complex work at the various engineering functions of aero engine manufacturers. Especially, efficiency of control system design and development in terms of cost, development period and technical relevance implies controlling diverse simulation and identification activities. The previous engine simulation has been accomplished within a limited analysis area such as fan, compressor, combustor, turbine, controller, etc. and this has resulted in improper engine performance and control characteristics because of limited interaction between analysis areas. In this paper, we propose a new simulation methodology for gas turbine engine performance analysis as well as its digital controller to solve difficulties as mentioned above. The novel method has particularities of (ⅰ) resulting in the integrated control simulation using almost every component/module analysis, (ⅱ) providing automated math model generation process of engine itself, various engine subsystems and control compensators/regulators, (ⅲ) presenting total sophisticated output results and easy understandable graphic display for a final user. We call this simulation system GT3GS (Gas Turbine 3D Graphic Simulator). GT3GS was built on both software and hardware technology for total simulation capable of high calculation flexibility as well as interface with real engine controller. All components in the simulator were implemented using COTS (Commercial Off the Shelf) modules. In addition, described here includes GT3GS main features and future works for better gas turbine engine simulation.