• Asian-Australasian Journal of Animal Sciences
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• v.19 no.4
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• pp.554-562
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• 2006

Two lines of mice, M16 selected for rapid growth and a randomly selected control ICR as well as their reciprocal crosses were used to study the effects of genotype on whole-body energetics and intestinal responses to monensin. Six mice, eight weeks of age, from each line or reciprocal cross were assigned to one of two treatments, 1) drinking water containing 20 mmol/L monensin dissolved in 0.5% V/V ethanol, and 2) drinking water containing 0.5% V/V ethanol (control) for two weeks. After 11 days (age of 9 weeks and 4 days), whole-body $O_2$ consumption was measured. At the end of two weeks, jejunal $O_2$ consumption, intestinal tissue composition and histomorphometrics as well as the rate and efficiency of glucose absorption were estimated. In comparison with the control, monensin administration in drinking water resulted in less daily water intake (13.4 vs. 15.5 ml/mouse, p<0.01), less protein to DNA ratio of jejunal mucosa (5.41 vs. 6.01 mg/mg, p<0.05), lower villus width (88 vs. $100{\mu}m$, p<0.05), and less jejunal tissue $O_2$ consumption enhancement by alcohol (7.2 vs. 10.5%, p<0.01) in mice. Other than those changes, monensin had little (p>0.05) effect on variables measured in either line of mice or their reciprocal cross. In contrast, the M16 line, selected for rapid growth, as compared to the ICR controls or the reciprocal crosses, had less initial (pre-monensin treatment) whole-body $O_2$ consumption per gram of body weight (1.68 vs. $2.11-2.34{\mu}mol/min{\cdot}g$ BW, p<0.01) as compared to the ICR and reciprocal crosses. In addition, the M16 mice exhibited greater growth (412 vs. 137-210 mg/d, p<0.05), better feed efficiency (41.7 vs. 19.9-29.3 mg gain/g feed, p<0.05), shorter small intestines adjusted for fasted body weight (1.00 vs. 1.22-1.44 cm/g FBW, p<0.05), wider villi (109 vs. $87-93{\mu}m$, p<0.05), more mature height of enterocytes (28.8 vs. $24.4-25.1{\mu}m$, p<0.05) and a lower rate (91 vs. $133-145{\eta}mol\;glucose/min{\cdot}g$ jejunum, p<0.05) and less energetic efficiency (95 vs. $59-72{\eta}mol$ ATP expended/${\eta}mol$ glucose uptake, p<0.05) of glucose absorption compared to the ICR line and the reciprocal cross. Monensin had little (p>0.05) effect on whole-body $O_2$ consumption and jejunal function, whilst selection for rapid growth resulted in an apparent down-regulation of intestinal function. These data suggest that genetic selection for increased growth does not result in concomitant changes in intestinal function. This asynchrony in the selection for production traits and intestinal function may hinder full phenotypic expression of genotypic growth potential.

• Journal of Bio-Environment Control
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• v.11 no.2
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• pp.81-87
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• 2002

This study was conducted to investigate the cooling efficiency and growth of tomatoes by root zone cooling device using a pad-box and cultivated system. The structure of the root zone cooling system using a pad-box was four piece of pads bonded an the side and a fan set at the bottom. Cool wind was generated by the outside air which was punched at intervals of 10 cm along three rows. Cold wind flowed to the root zone in the culture medium. The root zone cooling efficiency of cold wind generation by using a pad-box flowing through a wet-pad was determined. Major characteristic of this cuttural system consist of bed filled with a perlite medium and a ventilation pipe using PVC. The cold wind generation by a pad box (CWP) was compared to that of cold wind generation by a radiator (CWR), cold water circulation using a XL-pipe (CWX) and the control (non-cooling). When the temperature of water supplied was 16.2-18.4$^{\circ}C$, temperatures in the medium were 20.5~23.2$^{\circ}C$ for CWP 22.7~24.2$^{\circ}C$ for CWR, 22.8~24.27$^{\circ}C$ for CWX and 23.1~-29.6$^{\circ}C$ for the control. The results show that the cold wind temperature using the pad-box was lower by 1~2$^{\circ}C$ than that of cold water circulation in the XL-pipe and lower by 5~6$^{\circ}C$ than that of the control. Growth such as leaf length, leaf width, fresh weight and dry weight, was greater in three root zone cooling methods than in the control. Root activity was higher in the rat zone cooling methods than in the control. However, there was no significant difference among root zone cooling methods.

• Journal of The Korean Society of Agricultural Engineers
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• v.57 no.5
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• pp.51-60
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• 2015

In Korea, 69.4 % of duck farms had utilized conventional plastic greenhouses. In this facilities, there are difficulties in controlling indoor environments for raising duck. High rearing density in duct farms also made the environmental control difficult resulting in getting more stressed making their immune system weaker. Therefore, a facility is needed to having structurally enough solidity and high efficiency on the environmental control. So, new design plans of duck house have recently been conducted by National Institute of Animal Science in Korea. As a study in advance to establish standard, computational fluid dynamics (CFD) was used to estimate the aerodynamic problems according to the designs by means of overall and regional ventilation efficiencies quantitatively and qualitatively. Tracer gas decay (TGD) method was used to calculate ventilation rate according to the structural characteristics of duck houses including installation of indoor circulation fan. The results showed that natural ventilation rate was averagely 164 % higher than typically designed ventilation rate, 1 AER ($min^{-1}$). Meanwhile, mechanically ventilated duck houses made 81.2 % of summer ventilation rate requirement. Therefore, it is urgent to develop a new duck house considering more structural safety as well as higher efficiency of environmental control.

• Journal of agriculture & life science
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• v.46 no.3
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• pp.119-127
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• 2012

For the promotion of the evaporative cooling efficiency of hot air in greenhouse in summer, a fog sprayer consisted of a high volume spraying two-fluid nozzle with turbo fan and a blowing fan was set up at 2.2 m height from bottom of small glass greenhouse and tested to estimate the possibility of the greenhouse cooling. The mean droplet size and the volume sprayed by one of fog sprayer were $29{\mu}m$ and $160m{\ell}/min$. All the droplets sprayed and blown by the fog sprayer were evaporated within 2 m radius. The result from the cooling test that two sprayers set up in glass greenhouse of plane area $228m^2$ was represented lower cooling effect that the temperature and relative humidity of inside air of greenhouse were $28.8^{\circ}C$ and 87.5% when those of outside air of greenhouse were $30.2^{\circ}C$ and 81.2%. Through investigation of literatures and results of the cooling test, it was estimated that the water spraying rate of evaporative cooling of single span greenhouse with 50% light curtain and with air change rate of 1 volume/min was $10m{\ell}/min/m^2$ so that the inside air temperature may cool down $2{\sim}3^{\circ}C$ on the basis of $35^{\circ}C$ atmospheric temperature in summer of south korean area.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.11
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• pp.1529-1534
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• 2005

Individual-breed assignments were implemented in six swine populations using twenty six microsatellites recommended by the Food and Agriculture Organization and the International Society for Animal Genetics (FAO-ISAG). Most microsatellites exhibited high polymorphisms as shown by the number of alleles and the polymorphism information content. The assignment accuracy per locus obtained by using the Bayesian method ranged from 33.33% (CGA) to 68.47% (S0068), and the accumulated assignment accuracy of the top ten loci combination added up to 96.40%. The assignment power of microsatellites based on the Bayesian method had positive correlations with the number of alleles and the gene differential coefficient ($G_{st}$) per locus, while it has no relationship to genetic heterozygosity, polymorphism information content per locus and the exclusion probabilities under case II and case III. The percentage of corrected assignment was highest for the Bayesian method, followed by the gene frequency and distancebased methods. The assignment efficiency of microsatellites rose with increase in the number of loci used, and it can reach 98% when using a ten-locus combination. This indicated that such a set of ten microsatellites is sufficient for breed verification purposes.

• The KSFM Journal of Fluid Machinery
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• v.17 no.5
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• pp.43-53
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• 2014

This paper presents a the study on air curtain system of top and bottom bi-directional jet air discharge for blocking the spread of smoke in case of tunnel fire. The five kinds different air curtains of A, B, C, D, and E of models for various performance tested after manufactured. A results of the various performance test obtained the best efficiency from E model air curtain. And optimize the injection angle of the air curtain nozzle through the three-dimensional computational fluid dynamics (CFD) analysis and analyzed the effects of external pressure of tunnel. and also single factor design have been applied. At present, our attention is focused on the velocity distribution(flow width and flow position) of 1.5m on the ground in tunnel. Also, analyzed the influence of draft in the tunnel. Detailed effects of discharge angle of air curtain and velocity at nozzle exit are discussed.

• Bulletin of the Korean Chemical Society
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• v.31 no.7
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• pp.2025-2030
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• 2010

When $Cu^{2+}$ was used as an electron acceptor, removal of $Cu^{2+}$ was achieved from the synthesized wastewater (SW) in the cathode compartment of a microbial fuel cell (MFC). By addition of $KNO_3$, the different initial pH of the SW showed no effect on the removal efficiency of $Cu^{2+}$. For $Cu^{2+}$ concentration of 50 mg/L the removal efficiencies were found to be 99.82%, 99.95%, 99.58%, and 99.97% for the $KNO_3$ concentrations of 0, 50, 100 and 200 mM, and to be 99.4%, 99.9%, 99.7%, and 99.7% for pH values of 2, 3, 4, and 5, respectively. More than 99% $Cu^{2+}$ was removed for the $Cu^{2+}$ concentrations of 10, 50, and 100 mg/L, while only 60.1% of $Cu^{2+}$ was removed for the initial concentration of 200 mg/L (pH 3). The maximum power density was affected by both $KNO_3$ concentration and initial concentration of $Cu^{2+}$. It was increased by a factor of 1.5 (from 96.2 to 143.6 mW/$m^2$) when the $KNO_3$ concentration was increased from 0 to 200 mM (50 mg/L $Cu^{2+}$), and by a factor of 2.7 (from 118 to 319 mW/$m^2$) when $Cu^{2+}$ concentration was increased from 10 to 200 mg/L (pH 3).

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.9
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• pp.4807-4814
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• 2012

Purpose: The chemoresistance of human hepatocellular carcinoma (HCC) to cytotoxic drugs, especially intrinsic or acquired multidrug resistance (MDR), still remains a major challenge in the management of HCC. In the present study, possible mechanisms involved in MDR of HCC were identified using a 5-fluorouracil (5-FU)-resistant human HCC cell line. Methods: BEL-7402/5-FU cells were established through continuous culturing parental BEL-7402 cells, imitating the pattern of chemotherapy clinically. Growth curves and chemosensitivity to cytotoxic drugs were determined by MTT assay. Doubling times, colony formation and adherence rates were calculated after cell counting. Morphological alteration, karyotype morphology, and untrastructure were assessed under optical and electron microscopes. The distribution in the cell cycle and drug efflux pump activity were measured by flow cytometry. Furthermore, expression of potential genes involved in MDR of BEL-7402/5-FU cells were detected by immunocytochemistry. Results: Compared to its parental cells, BEL-7402/5-FU cells had a prolonged doubling time, a lower mitotic index, colony efficiency and adhesive ability, and a decreased drug efflux pump activity. The resistant cells tended to grow in clusters and apparent changes of ultrastructures occurred. BEL-7402/5-FU cells presented with an increased proportion in S and G2/M phases with a concomitant decrease in G0/G1 phase. The MDR phenotype of BEL-7402/5-FU might be partly attributed to increased drug efflux pump activity via multidrug resistance protein 1 (MRP1), overexpression of thymidylate synthase (TS), resistance to apoptosis by augmentation of the Bcl-xl/Bax ratio, and intracellular adhesion medicated by E-cadherin (E-cad). P-glycoprotein (P-gp) might play a limited role in the MDR of BEL-7402/5-FU. Conclusion: Increased activity or expression of MRP1, Bcl-xl, TS, and E-cad appear to be involved in the MDR mechanism of BEL-7402/5-FU.

• 한국신재생에너지학회:학술대회논문집
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• 2005.06a
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• pp.397-402
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• 2005

Photovoltaic (PV) module can generate electricity using sunlight without causing any environmental degradation. Due to higher fossil fuel prices and environmental awareness, PV applications are becoming more popular as clean source of electricity generation. PV output is sensitive to the operating temperature and can be drastically affected in Building Integrated PV (BIPV) systems. PV Solarwall (SWPV) combination and PV systems have been evaluated in this study for improvement in electrical output and system costs. PV modules under forced ventilation. A 75W polycrystalline silicon PV module was fixed on SW in front of the ventilation fan as it was indicated to be the coolest position on the SW in phoenix simulations. The effectiveness of cooling by means of the forced ventilating air stream has been studied experimentally. The results indicate that there appears to be significant difference in temperature as well as electricity output comparing the SWPV and BIPV options. Electrical output power recovered is about $4\%$ during the typical day of the month of February. RETScreen(R) analysis of a 3kW PV system hypothetically located at Taegu has shown that with typical temperature reduction of $15^{{\circ}C$, it is possible to reduce the simple payback periods by one year. The work described in this paper may be viewed as an appraisal of a SWPV system based on its improved electrical and financial performances due to its ability to operate at relatively lower temperatures.

• Korean Journal of Poultry Science
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• v.10 no.2
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• pp.97-101
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• 1983

A study was conducted to investigate possibility of utilizing dried citrus peel (DCP) as an ingridient of broiler diets. Fresh citrus peels were collected from a citrus processing plant, and were sun-dried and ground. Both chemical analyses and a feeding trial were carried out. DCP was analyzed for proximate nutrients, amino acids and some minerals. In the feeding trial, a total of 192 day-old female broiler chicks of Manor strain was divided into 16 groups of 12 birds each. Each group was fed one of the 4 different levels(0, 2, 4 and 6%) of DCP replacing an equivalent amount of wheat bran in the diet with 4 replications for 6 weeks. Body weight gain, feed intake and feed efficiency of broilers fed different levels of DCP showed no significant differences among treatments. Immediately after termination of the feeding trial, cach bird was examined for shank color using Roche's Egg Yolk Color Fan. Shank color index of birds increased consistently (P＜0.05) as the level of DCP fed increased, indicating that DCP can be used as a source of pigments. It was concluded from the results that DCP could be used up to 6% in place of wheat bran in broiler diets without adverse effects.