• Asian Pacific Journal of Cancer Prevention
• /
• 제14권10호
• /
• pp.5901-5904
• /
• 2013

Objective: To compare the effectiveness of different methods of recall for repeat Pap smear among women who had normal smears in the previous screening. Design: Prospective randomized controlled study. Setting: All community clinics in Klang under the Ministry of Health Malaysia. Participants: Women of Klang who attended cervical screening and had a normal Pap smear in the previous year, and were due for a repeat smear were recruited and randomly assigned to four different methods of recall for repeat smear. Intervention: The recall methods given to the women to remind them for a repeat smear were either by postal letter, registered letter, short message by phone (SMS) or phone call. Main Outcome Measures: Number and percentage of women who responded to the recall within 8 weeks after they had received the recall, irrespective whether they had Pap test conducted. Also the numbers of women in each recall method that came for repeat Pap smear. Results: The rates of recall messages reaching the women when using letter, registered letter, SMS and phone calls were 79%, 87%, 66% and 68%, respectively. However, the positive responses to recall by letter, registered letter, phone messages and telephone call were 23.9%, 23.0%, 32.9% and 50.9%, respectively (p<0.05). Furthermore, more women who received recall by phone call had been screened (p<0.05) compared to those who received recall by postal letter (OR=2.38, CI=1.56-3.62). Conclusion: Both the usual way of sending letters and registered letters had higher chances of reaching patients compared to using phone either for sending messages or calling. The response to the recall method and uptake of repeat smear, however, were highest via phone call, indicating the importance of direct communication.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권24호
• /
• pp.10643-10646
• /
• 2015

Background: To study the relationship between mammographic findings and clinical/pathologic features in women with 1-15mm sized invasive breast cancer. Materials and Methods: We investigated a consecutive series of 134 cases diagnosed in Tianjin Medical University Cancer Institute and Hospital in 2007. Mammographic findings were classified into five groups as follows :1) stellate mass without calcification; 2) non-stellate mass without calcification; 3) intermediate suspicious calcification with or without associated mass; 4) higher probability malignant calcification with or without associated mass; 5) focal asymmetry/distortion without associated calcification. Associations between mammographic and clinical/pathological features (menopause status/family history/histologic grade/lymph node status and ER/PR/HER2 status) was analyzed through logistic regression and chi square tests. Results: Compared to the stellate mass without calcification group, higher probability malignant calcification patients were associated significantly with a positive lymph node status, always presenting in patients who were non-menopausal and with a family history of carcinoma. Conclusions: Higher probability malignant calcifications with or without associated tumor masses are associated with clinical/pathologic features of poor prognosis.

• Journal of Microbiology and Biotechnology
• /
• 제32권8호
• /
• pp.1064-1071
• /
• 2022

Arabinogalactans have diverse biological properties and can be used as pharmaceutical agents. Most arabinogalactans are composed of β-(1→3)-galactan, so it is particularly important to identify β-1,3-galactanases that can selectively degrade them. In this study, a novel exo-β-1,3-galactanase, named PoGal3, was screened from Penicillium oxalicum sp. 68, and hetero-expressed in P. pastoris GS115 as a soluble protein. PoGal3 belongs to glycoside hydrolase family 43 (GH43) and has a 1,356-bp gene length that encodes 451 amino acids residues. To study the enzymatic properties and substrate selectivity of PoGal3, β-1,3-galactan (AG-P-I) from larch wood arabinogalactan (LWAG) was prepared and characterized by HPLC and NMR. Using AG-P-I as substrate, purified PoGal3 exhibited an optimal pH of 5.0 and temperature of 40℃. We also discovered that Zn²⁺ had the strongest promoting effect on enzyme activity, increasing it by 28.6%. Substrate specificity suggests that PoGal3 functions as an exo-β-1,3-galactanase, with its greatest catalytic activity observed on AG-P-I. Hydrolytic products of AG-P-I are mainly composed of galactose and β-1,6-galactobiose. In addition, PoGal3 can catalyze hydrolysis of LWAG to produce galacto-oligomers. PoGal3 is the first enzyme identified as an exo-β-1,3-galactanase that can be used in building glycan blocks of crucial glycoconjugates to assess their biological functions.

• Journal of Microbiology and Biotechnology
• /
• 제24권11호
• /
• pp.1484-1489
• /
• 2014

A novel esterase gene, est01, was successfully unearthed from a biogas digester microbiota metagenomic library. The 1,194 bp est01 gene encodes a protein of 44,804 Da (designated Est01). The amino acid sequence of Est01 shows only moderate (33%) identity to a lipase/esterase. Phylogenetic analysis and biochemical characterization confirmed that Est01 is a new member of family VIII esterases. The purified Est01 from recombinant Escherichia coli BL21 (DE3) showed high hydrolytic activity against short-chain fatty acid esters, suggesting that it is a typical carboxylesterase rather than a lipase. Furthermore, the Est01 was even active at $10^{\circ}C$ (43% activity remained), with the optimal temperature at $20^{\circ}C$, and had a broad pH range from 5.0 to 10.0, with the optimal pH of 8.0. These properties suggest that Est01 is a cold-adaptive esterase and could have good potential for low-temperature hydrolysis application.

• 한국교육학연구
• /
• 제18권3호
• /
• pp.157-182
• /
• 2012

이 연구는 2006년 이후 급속하게 전개되어 온 한국사회 이주배경 아동 청소년 정책의 가시적. 비가시적 아젠다를 비판적으로 분석하여 새로운 정책 패러다임을 모색하려는 목적에서 이루어졌다. 그동안 다양한 차원에서 이루어져 온 이주배경 아동 청소년 정책은 한국사회의 변화를 촉발시키며 다문화사회의 저변을 구축하는 긍정적인 기능을 수행해왔다. 그럼에도 불구하고, 이주배경 아동 청소년에 대한 낙인과 부정적인 이미지의 확산 등 정책의 역효과 문제, 이들에 대한 집중지원으로 인한 일반 소외계층 아동 청소년에 대한 역차별 문제 등으로 많은 비판의 대상이 되어 왔다. 이 연구는 이러한 현실을 염두에 두고 성장기인 이주배경 아동 청소년에 직접적인 영향을 미치는 가족과 교육 정책 아젠다를 분석하여 그 의의와 한계 그리고 시사점과 향후 방향성을 제시하였다.

• 한국학교ㆍ지역보건교육학회지
• /
• 제11권1호
• /
• pp.41-55
• /
• 2010

Objectives: This study was conducted to identify the characteristics of health behavior and self-esteem and the relationship between the two factors among elementary school students with multi-cultural family background. Participants: Survey participants were 87 multi-cultural family children who lived in Seoul and Kyoungi areas and were registered in the local elementary schools. Methods: Data collection was conducted by face-to-face interview survey. Elementary education majored undergraduate students who participated in the education support program for multi-cultural family children in the Ministry of Education and Science and primary researcher of this study interviewed the target students and finished the survey questionnaire based on the students' responses. Survey included general characteristics, multi-cultural family characteristics, self-esteem, and (physical, mental, and social) health behavior. Results: 1. The self-esteem scores of the multi-cultural families children were greater in girl than boys among the children whose parents were graduated from college or more, and whose father had his job. The participants' health behavior scores were different by mothers' educational level. 2. The better they speak in English the greater the self-esteem scores. There was no statistical difference in health behavior scores by the participants' multi-cultural family background. 3. Self-esteem had moderate level correlation with health behavior (r= .56, p= .00) including all sub-categories of physical, mental, and social health. Conclusions: Positive self-esteem level is more sensitive to explain multi-cultural family children's health behaviors, which means that self-esteem is an important factor determining multi-cultural children's health behaviors and their health status in future. Therefore, more research to identify the factors related to health behaviors should be supported and the health promotion programs utilizing self-esteem should be developed for the child and youth with multi-cultural family backgrounds.

• 가족자원경영과 정책
• /
• 제26권1호
• /
• pp.59-73
• /
• 2022

이 연구는 질적 연구 방법론에 의한 현장사례연구로서, 공동육아나눔터의 주요 기능과 운영상의 문제 및 활성화 조건을 탐구한 것이다. 지역(대도시·중소도시·군단위), 개소 시기, 운영기관, 위치, 맞벌이자녀초등전담돌봄 여부, 2020년 돌봄공동체시범사업 참여 여부 등을 고려하여 전국 8지역을 사례로 선정하였다. 공동연구자가 각 센터 현장을 함께 방문하여 센터장 혹은 전담직원을 대상으로 반구조화된 질문지를 활용하여 심층면접을 진행하였다. 면접자료를 전사하여 34개의 하위범주와 12개의 범주를 구성하고, 공동육아나눔터의 주요기능과 운영상의 어려움 그리고 활성화 조건이라는 세 가지 주제를 도출하였다. 이러한 주제를 토대로 공동육아나눔터 활성화방안을 제안하였다.

• Journal of Microbiology and Biotechnology
• /
• 제24권2호
• /
• pp.133-143
• /
• 2014

The development of the gut is controlled and modulated by different interacting mechanisms, such as genetic endowment, intrinsic biological regulatory functions, environment influences and last but no least, the diet influence. In this work, we compared the fecal microbiota of breast-fed (BF), formula-fed (FF), and mixed-fed (MF) infants from Hebei Province, China. By using high-throughput 16S rDNA sequencing analyses, we found some differences in gut microbiota in the three groups. Firmicutes and Proteobacteria were the dominant bacteria at the phylum level in the three groups, where FF infants showed a significant depletion in Bacteroidetes (p < 0.001) and Actinobacteria (p < 0.05). Enterobacteriaceae was the dominant bacteria at the family level in the three groups, but FF infants showed higher Enterobacteriaceae enrichment than BF and MF infants (p < 0.05); the abundance of the Bifidobacteriaceae was only 8.16% in the feces of BF infants, but higher than in MF and FF infants (p < 0.05). The number of genera detected (abundance >0.01%) in BF, MF, and FF infants was only 15, 16, and 13, respectively. This study could provide more accurate and scientific data for the future study of infant intestinal flora.

• Asian-Australasian Journal of Animal Sciences
• /
• 제20권6호
• /
• pp.811-816
• /
• 2007

Adipocyte fatty acid-binding protein (A-FABP), which belongs to the FABP family, plays an essential role in long-chain fatty acid uptake and metabolic homeostasis, especially in adipose tissue. The pattern of A-FABP gene mRNA expression in different growth stages and its relation to intramuscular fat (IMF) accretion in pigs was studied. Fifteen female $Duroc{\times}Landrace{\times}Yorkshire$ pigs in five groups of three pigs each, weighing 1, 30, 50, 70 and 90 kg were used to study developmental gene mRNA expression of A-FABP in various adipose tissues by means of semi-quantitative RT-PCR. Results showed that A-FABP mRNA levels in subcutaneous and ventral adipose tissues first increased from 1 to 50 kg, then gradually declined from 50 to 90 kg. Moreover, the rank order of A-FABP mRNA levels determined in three adipose tissues was as follows: subcutaneous adipose>ventral adipose>mesenteric adipose. A-FABP mRNA expression in mesenteric adipose tissue was constant during development. In addition, a positive correlation from 1 to 50 kg BW pigs and a negative correlation from 50 to 90 kg BW between A-FABP mRNA levels in subcutaneous and ventral adipose and IMF content were found.

• Mycobiology
• /
• 제44권2호
• /
• pp.79-84
• /
• 2016

A coelomycete with characters resembling the asexual morphs in the family Botryosphaeriaceae was isolated from a fallen leaf of an orchid collected in Thailand. Morphological and phylogenetic analyses placed the strain in Neoscytalidium. Phylogenetic relationships among Neoscytalidium species were inferred by analyzing internal transcribed spacers and large subunit of rRNA sequence data and indicate that our strain is a new species, which is introduced and illustrated herein as Neoscytalidium orchidacearum sp. nov.