The influence of incubation temperature, pH and media components on bacteriocin production by Lactobacillus plantarum K11 were investigated. The highest activity was observed in MRS broth, but no bacteriocin activity was obtained in TSB. The bacteriocin was produced from the exponential growth phase and its activity also reached a maximum in MRS broth, but then dropped after 16 hr because of degradation by extracellular proteolytic enzymes or exhaustion of medium nutrients. The optimal temperature and pH for production of bacteriocin were $37^{\circ}C$ and pH 7.0 in MRS broth, respectively. The addition of 0.5 or 1.0% glucose and $0.5{\sim}1.5%$ lactose to MRS resulted in the increase of the bacteriocin production. With 0.5% NaCl and $K_2HPO_4$, the activities were significantly higher than that of control, respectively. However, increasing nitrogen sources such as beef extract, casein, and tryptone and salts such as $NH_4PO_4$, $MgSO_47H_2O$, and $MnSO_4H_2O$, had detected a negative influence upon the bacteriocin production. Consequently, because the bacteriocin produced by L. plantarum K11 was affected by various incubation conditions, the bacteriocin activity of L. plantarum K11 applied in food as a novel starter will be dependent on environmental factors such as fermentation conditions and food ingredients.
Differential tolerance of rice varieties, Tongil and Mangyung(or Milsung) varieties, against herbicides combined with Simetryne was investigated by the rate of application in 4 places; Jeonju, Gwangju, Iri and Milyang, whose soil textures were Lic, Sic, CL and Lic respectively. The results are summarized as follows: 1) Jeonju a. Initial crop injury At the rate of 2kg/10a prod. no initial crop injury was caused(both to Mangyung and Tongil varieties) on light clay soil, even when treated at high temperature of $30^{\circ}C$ and above. Crop injury was first caused at 3kg/l0a prod. to Tongil variety and the damage increased in proportion to the increase of application rate, while no crop injury was caused to Mangyung variety even at high rate of 5kg/l0a prod. b. Yield Tongil variety: Under high temperature condition in a vinyl house no statistical difference was observed between treated and hand weeding plot at the rates of 3kg/l0a prod. and below. Under air temperature conditions no statistical difference was observed between treated and hand weeding plot at the rates of 4kg/l0a prod. and below. Mangyung variety: No statistical difference was observed between treated and hand weeding plot at the rates of 5kg/l0a prod. and below both under air and high temperature conditions. 2) Gwangju a. Initial crop injury Although almost the same tendency as in Jeonju was observed, a little lighter crop injury was observed in general in Gwangju than in Jeonju at the time of application. And so, no crop injury was caused to Tongil variety until 4kg/l0a prod. and above. b. Yield Tongil variety: No statistical difference was observed between treated and hand weeding plot at 4kg/l0a prod. and below. Mangyung variety: No statistical difference was observed between treated and hand weeding plot at 4kg/l0a prod. and below. 3) Iri No crop injury was caused both to Tongil and Mangyung varieties at 4kg/l0a prod. and below. Also, no reduction of grain yield was observed. 4) Milyang A little lighter crop injury was observed in general. No crop injury was caused to Milsung variety at 5kg/l0a prod. ; Only a slight crop injury was caused to Tongil variety. Yield: No statistical difference was observed between treated and hand weeding plot both of Tongil and Milsung varieties even at 5kg/l0a prod. and below.
Seasonal changes of biomass and cell size of bacteria and protozoa, and factors affecting their distribution in Lake Paldang and Kyungan Stream were analyzed from April to December, 2005. Bacterial abundance at Paldang Dam and Kyungan Stream was similar, but it did not much increase during hot summer period. Protozoan carbon biomass was much greater at Kyungan Stream compared to Paldang Dam. HNAN generally accounted for the majority of total protozoan biomass, but ciliates made up the highest proportion in April and November at Paldang Dam and June at both sites. PNAN showed low biomass at both sites, but it was high during spring and fall season. Small-sized HNAN ($3{\sim}7\;{\mu}m$) numerically predominated the protozoan community at both sites. Average cell size of HNAN was bigger at Kyungan Stream where nutrients concentration was much higher than Paldang Dam. Average cell size of ciliates varied seasonally; it was relatively small during the summer. HNAN biomass significantly correlated with Chl-a concentration and ciliates biomass at Paldang Dam, indicating that HNAN increase might link to the ciliates increase. At Kyungan Stream, HNAN biomass showed a significant relationship with PNAN biomass, and Chl-a concentration was closely related with both of HNAN and PNAN biomass. Ciliate biomass showed significant relationship with nutrient (TN, TP) and particulate matter (SS) only at Kyungan Stream. At both sites, protozoan biomass was significantly correlated with bacterial biomass, and ciliates were additionally related flagellates. High biomass of microbial components and the close relationships among them suggest that the energy transfer through the microbial loop may important in the plankton food web of Lake Paldang ecosystem.
The effects of fermentation temperature$(0{\sim}l5^{\circ}C)$ and salt concentration$(1.5{\sim}4.0%)$ on the fermentation property of Chinese cabbage Kimchi were analyzed by response surface methodology. The pH decreased and acidity increased with increasing fermentation time. The reduction and increment velocities of pH and acidity were increased by increasing fermentation temperature and decreasing salt concentration. The optimum pH 4.2 was reached within $14{\sim}24$ days at $5{\sim}15^{\circ}C$, while pHs of 24 days at $0{\sim}5^{\circ}C$ were still lower value than 4.2. The effect of salt concentration more affected terminal fermentation period than initial fermentation period. The maximum edible acidity, 0.75%, was reached within 8 days at $15^{\circ}C$, while acidifies of 24 days at $0^{\circ}C$ were $0.35{\sim}0.43%$. The effects of salt concentration at $0^{\circ}C$ was higher than those at $15^{\circ}C$. The fermentation time, fermentation temperature and salt concentration were the first, second and third affecting factors on the pH and acidity of Kimchi. Based on the coefficients of determination, pH and acidity were highly fitted to the experimental data$(r^2>0.9276)$. For the suitable acidity range, $0.40{\sim}0.75%$, the edible period of Kimchi at $15^{\circ}C,\;10^{\circ}C\;and\;5^{\circ}C$ were 4 days, 10 days and 18 days at the 2.75% of salt concentration, respectively. The edible period increased from 14 days to 19 days with increased salt concentration from 1.50% to 4.00% at $5^{\circ}C$ of fermentation temperature.
Objects : This study was carried out to investigate characteristics of patients who need hypnotics on the night before elective surgery as well as contributing variables for the necessity of hypnotics. Methods : After reviewing the clinical charts of patients who were scheduled to receive surgery by general anesthesia the following day, researchers had semi structural interviews with patients. In addition, Spielberger's State-Trait Anxiety Inventory(SSTAI), Beck Depression Inventory(BDI), Zung's Self-Rating Pain and Distress Scale(ZPDS), and Presleep and Postsleep Questionnaires were administered to patients. A total of 167 patients, who gave reliable information, were divided into two groups based on subjective judgement regarding the necessity for hypnotics on the night before surgery; 29 eligibles for hypnotics and 138 non-eligibles for hypnotics. Demographic and clinical characteristics of patients, some possible factors affecting sleep, psychological characteristics of patients and daytime status and nighttime sleep before surgery were compared between the two groups. In addition, discriminant function analysis was done to find the variables which would best discriminate among patients who differ in terms of necessity for hypnotics on the night before surgery. Results : There was no difference in demographic and clinical characteristics between the two groups; however, the satisfaction level with ward environment was significantly lower in the eligible group for hypnotics than the non-eligible group. Psychologically, the eligible group for hypnotics, compared to the non-eligible group, showed significantly more severe depression, pain, and distress; whereas anxiety level was not different between the two groups. For nighttime sleep before surgery, the eligible group for hypnotics, compared to the non-eligible group, expected poorer sleep before retiring and in fact, reported poorer sleep the following morning. In discriminant function analysis, 'expectation for sleep' and 'pain and distress' were the most potent contributors to discriminate the necessity of hypnotics. Conclusion : For the improvement of the patient's sleep on the night before elective surgery, giving hypnotics and/or analgesics should be determined by patient's opinion about the necessity of the drugs rather than by the therapist's own judgement or any other objective indices.
The purpose of this study was to compare and analyze the differences in scan time, signal-to-noise ratio (SNR), and contrast-to-noise ratio (CNR) in the third lumbar vertebral region including the back fat, spinal cord, and cerebrospinal fluid using the mDixon, T2 TSE, and T2 spectral pre-saturation with inversion-recovery (SPIR) techniques. With the factors affecting the SNR fixed, the lumbar sagittal plane images of 30 adults were compared on mDixon, T2 TSE, and T2 SPIR imaging tests. The test times for mDixon, T2 TSE, and T2 SPIR were 115 seconds, 60 seconds, and 60 seconds, respectively. The mDixon T2 images showed higher SNR than the T2 TSE images at the third lumbar vertebral region (p<0.05), lower SNR in the back fat and cerebrospinal fluid (p<0.05) areas, and comparable SNR in the spinal cord (p>0.05). The CNR between the third lumbar vertebral area and back fat was higher in the mDixon T2 images, and the CNR of the cerebrospinal fluid and spinal cord images was higher in the T2 TSE images (p<0.05). The mDixon T2 FS images CNR was lower for the 3rd lumbar vertebral body region and back fat than the T2 SPIR images, and higher for the spinal cord and cerebrospinal fluid images (p<0.05). The CNR between the third lumbar body and back fat areas was higher in the mDixon T2 FS images (p<0.05), and there was no difference in the CNR in the images of the cerebrospinal fluid and the spinal cord (p>0.05). It is difficult to determine whether the mDixon technique is superior to the conventional T2 TSE and T2 SPIR techniques in terms of test time, SNR, and CNR. This study was confined to patients with simple lower back pain and was limited by controlled experimental conditions. Studies using clinically applied protocols are warranted in the future.
Lee, Bae Hun;Kim, Ji Yung;Sung, Kyung Il;Kim, Byong Wan
Journal of The Korean Society of Grassland and Forage Science
/
v.39
no.2
/
pp.89-96
/
2019
This study was conducted to investigate 106 grassland farms in six provinces including Chungcheongbuk-do, Gangwon-do, Gyeonggi-do, Gyeongsangbuk-do, Gyeongsangnam-do, Jeju-do, and Jeollanam-do to present their problems and causes by surveying the actual state of grassland farms during three years(2014~2016). The grassland survey was divided into three categories; used as the perennial grasses and annual forage crops (Complied with Grassland Act), not used as the perennial grasses and annual forage crops (Not complied with Grassland Act) and failed to meet the farmer and some items are missing or inaccurate (Insufficient contents). Among the surveyed grassland farm, 68 farms (64.2 %) were complied with Grassland Act but 30 farms (28.3 %) were not complied with Grassland Act. Especially, the 8 farms (26.7 %) not complied with Grassland Act used the grassland as other purposes such as tree growing, golf club and swine farm etc.. Therefore, strict on-site investigation by local governments is required to prevent the grassland from being used by illegal purposes. And there's a strong likelihood that 5 farms (62.5 %) avoided the survey violate the positive law. Grassland grades used by the local administrative agencies were not influenced by the factors affecting the yield (existence and non-existence of overseeding and fertilization by grassland grade, soil pH and organic matter content). This results suggest that there is a fundamental problem on the current grassland grade system based on the yield and the irregular time of investigation and lack of on-site investigation are another causes for inaccurate grassland grade. Therefore, the new method evaluating grassland grades which is not based on yield and the thorough on-site investigation by local administrative agencies are necessary when the grassland grade is evaluated.
Song, Ki Eun;Choi, Jae Eun;Jung, Jae Gyeong;Ko, Jong Han;Lee, Kyung Do;Shim, Sang-In
KOREAN JOURNAL OF CROP SCIENCE
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v.66
no.4
/
pp.307-317
/
2021
In recent years, global warming has led to frequent climate change-related problems, and elevated temperatures, among adverse climatic factors, represent a critical problem negatively affecting crop growth and yield. In this context, the present study examined the physiological traits of wheat plants grown under high temperatures. Specifically, the effects of elevated temperatures on seed development after heading were evaluated, and the vegetation indices of different organs were assessed using hyperspectral analysis. Among physiological traits, leaf greenness and OJIP parameters were higher in the high-temperature treatment than in the control treatment. Similarly, the leaf photosynthetic rate during seed development was higher in the high-temperature treatment than in the control treatment. Moreover, temperature by organ was higher in the high-temperature treatment than in the control treatment; consequently, the leaf transpiration rate and stomatal conductance were higher in the control treatment than in the high-temperature treatment. On all measuring dates, the weight of spikes and seeds corresponding to the sink organs was greater in the high-temperature treatment than in the control treatment. Additionally, the seed growth rate was higher in the high-temperature treatment than in the control treatment 14 days after heading, which may be attributed to the higher redistribution of photosynthates at the early stage of seed development in the former. In hyperspectral analysis, the vegetation indices related to leaf chlorophyll content and nitrogen state were higher in the high-temperature treatment than in the control treatment after heading. Our results suggest that elevated temperatures after the booting stage positively affect wheat growth and yield.
Changes in consumer needs and behaviors according to lifestyle changes lead to consumption culture, affecting the automobile market. However, research and research to provide options tailored to the lifestyle of consumers in related markets are still insufficient. Focusing on pickup truck accessories applied to pickup trucks that reflect lifestyle the most among vehicle types, this study first examined the theoretical background of the aftermarket market and lifestyle of pickup trucks. Second, through image mapping, the market possibilities and opportunity factors of pickup trucks were discovered through market size analysis and possibilities, and through this, user types could be classified. Third, interviews were conducted with those representing user types, the contents were organized, and interviews were conducted centering on related groups to create a persona of a user group, and what needs each group's persona wanted. Finally, a design concept suitable for the issue keywords and insights derived for each user lifestyle type was presented. In this study, the user type was divided into ① outdoor activity type, ② hobby activity type, and ③ small-scale work type, and a design case study was conducted by applying the concept suitable for the keyword for each group. For the outdoor activity type, a variable storage structure and a living space-type accessory design were presented, and for the hobby type, a modular decktop design and a sports coupe-type hardtop design were presented. For the small business type, a partition that is easy to fix the load and a stepper design that is easy to board the cargo box were presented. It is expected that the size of the pickup truck aftermarket will be expanded by diversifying the option designs that users want by lifestyle by applying them to the development of pickup truck accessories that fit the lifestyle of pickup truck users in the automobile market, which is currently mass customized.
The forest in the lower Tumen River serves as an important ecosystem spanning the territories of North Korea, Russia, and China, and it provides habitat and movement corridors for diverse mammals, including the endangered Amur tiger (Panthera tigris) and Amur leopard (Panthera pardus). This study focuses on the Mijiang area, situated as a potential ecological corridor connecting North Korea and China in the lower Tumen River, playing a crucial role in conserving and restoring the biodiversity of the Korean Peninsula. This study aimed to identify mammal species and estimate their relative abundance, occupancy, and distribution based on the 48 camera traps installed in the Mijiang area from May 2019 to May 2021. The results confirmed the presence of 18 mammal species in the Mijiang area, including large carnivores like tigers and leopards. Among the dominant mammals, four species of ungulates showed high occupancy and detection rates, particularly the Roe deer (Capreolus pygargus) and Wild boar (Sus scrofa). The roe deer was distributed across all areas with a predicted high occupancy rate of 0.97, influenced by altitude, urban residential areas, and patch density. Wild boars showed a predicted occupancy rate of 0.73 and were distributed throughout the entire area, with factors such as wetland ratio, grazing intensity, and spatial heterogeneity in aspects of the landscape influencing their occupancy and detection rates. Sika deer (Cervus nippon) exhibited a predicted occupancy rate of 0.48, confined to specific areas, influenced by slope, habitat fragmentation diversity affecting detection rates, and the ratio of open forests impacting occupancy. Water deer (Hydropotes inermis) displayed a very low occupancy rate of 0.06 along the Tumen River Basin, with higher occupancy in lower altitude areas and increased detection in locations with high spatial heterogeneity in aspects. This study confirmed that the Mijiang area serves as a habitat supporting diverse mammals in the lower Tumen River while also playing a crucial role in facilitating animal movement and habitat connectivity. Additionally, the occupancy prediction model developed in this study is expected to contribute to predicting mammal distribution within the disrupted Tumen River basin due to human interference and identifying and protecting potential ecological corridors in this transboundary region.
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