• 한국잡초학회지
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• 제30권2호
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• pp.122-134
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• 2010

본 연구의 목적은 다양한 생물종의 Protox 유전자 과다발현 제초제 저항성 형질전환 벼의 저항성 수준과 생육저해 원인이 생육시기별 tetrapyrrole 중간물질 축적과 대사 경로 물질, 활성산소 발생, 지질과산화 작용 및 항산화 효소 능력과 관련성이 있는지를 조사하는데 있다. Myxococcus xanthus(MX, MX1, PX), Arabidopsis thaliana(AP31, AP36, AP37) 및 인간(H45, H48, H49) Protox 과다발현 형질전환벼는 비형질전환벼에 비해 oxyfluorfen에 각각 43~65, 41~72 및 17~70배 저항성을 보였다. 다양한 생물종의 Protox 유전자 과다발현 제초제 저항성 형질전환 벼 중에 일부 라인은 다른 광조건하에서 정상적인 생육을 보인 반면에 일부 라인은 초장 및 생체중 감소가 야기되었다. 그러나 일관성 있게 파종 후 7일과 14일에 초장의 감소가 야기 되었던 형질전환 라인은 AP37뿐이었다. 또한 이들 형질전환 벼 라인들은 저광 및 암조건보다 고광조건하에서 초장 및 생체중 감소가 뚜렷하였다. 파종 후 7일째 Proto IX 축적은 AP31, AP37 및 H48에서 비형질전환벼에 비해 유의적으로 많았고, 파종 후 14일째에는 PX, AP37 및 H48에서 유의적으로 많았다. 파종 후 7일째 Mg-Proto IX은 일부 라인(H41과 H48)을 제외하고 그리고 Mg-Proto IX monomethyl ester는 MX, MX1, PX를 제외한 형질전환 라인 간에 차이가 없었다. 비록 terapyrrole 중간물질이 축적 되었더라도 이들 축적량은 Protox 과다발현 형질전환벼의 생육을 저해 하는데 충분하지 않았을 것으로 생각된다. 또한 활성산소종 ($H_2O_2$와 ${O_2}^{{\cdot}_-}$), MDA, ALA 합성 및 엽록소 함량에서도 형질전환 라인간에 유의적인 차이가 인정되지 않아 tetrapyrrole 축적량이 형질전환 벼의 생육저해에 충분하지 못했음을 확인할 수 있었다. 따라서 일부 형질전환벼에서 초기 생육저해는 어떤 단일 요인에 기인되는 것보다 복합적인 요인에 의해 기인되는 것으로 생각된다.

• Archives of Pharmacal Research
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• 제27권6호
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• pp.619-623
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• 2004

Tissue factor (TF, tissue thromboplastin) is a membrane bound glycoprotein, which acceler-ates the blood clotting, activating both the intrinsic and the extrinsic pathways to serve as a cofactor for activated factor VII (Vila). The TF-factor Vila complex (TF/VIIa) proteolytically activates factors IX and X, which leads to the generation of thrombin and fibrin clots. In order to isolate TF inhibitors, by means of a bioassay-directed chromatographic separation technique, from the leaves of Eriobotrya japonica Lindley (Rosaceae), a known sesquiterpene glycoside (2) and ferulic acid (3) were isolated as inhibitors that were evaluated using a single-clotting assay method for determining TF activity. Another sesquiterpene glycoside (1) was also isolated but was inactive in the assay system. Compound 3 was yielded by alkaline hydrolysis of compound 2. The structures of compounds 1, 2, and 3 were identified by means of spectral analysis as $3-O-{\alph}-L-rhamnopyranosyl-(1{\rightarrow}4)-a-L-rhamnopyranosyl-(1{\rightarrow}2)-[{\alph}-L-rhamnopyrano-syl-(1{\rightarrow}6)]-{\beta}-D-glucopyranosyl nerolidol$ (1), $3-O-{\alph}-L-rhamnopyranosyl-(1{\rightarrow}4)-{\alph}-L-rhamnopyr-anosyl-(1{\rightarrow}2)-[{\alph}-L-(4-trans-feruloyl)-rhamnopyranosyl-(1{\rightarrow}6)]-{\beta}-D-glucopyranosyl$ nerolidol (2) and ferulic acid (3), respectively. Compounds 2 and 3 inhibited 50% of the TF activity at con-centrations of 2 and $369{\;}\mu\textrm{m}/TF$ units, respectively.

• 대한본초학회지
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• 제23권4호
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• pp.191-196
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• 2008

Objectives: This research was investigated to find out the effect of the anticoagulant Perillae folium extract. Methods: To examine an active effect of anticoagulation in Perillae folium extract, the study measured Prothrombin time(PT) and activated partial thromboplastin time(APTT) of human plasma in vitro and measured bleeding time and arterio-venous shunt model in rats in vivo. Results: Bleeding time of Perillae folium extract in vivo had a significant increase 1.6 times and thrombus weight of Perillae folium extract had a significant reduction of thrombus weight as 68%. Perillae folium extract had an effect of anticoagulation by operating on extrinsic pathway factor II, V, VII, X and intrinsic pathway factor VIII, IX, X, VI, VII in the coagulation system. Conclusions: Considering the above mentioned results, it is judged that a Perillae folium extract has a control effect of thrombus creation.

• BMB Reports
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• 제35권2호
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• pp.199-205
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• 2002

An anticoagulant protein was purified from the edible portion of a blood ark shell, Scapharca broughtonii, by ammonium sulfate precipitation and column chromatography on DEAE-Sephadex A-50, Sephadex G-75, DEAE-Sephacel, and Biogel P-l00. In vitro assays with human plasma, the anticoagulant from 'S. broughtonii, prolonged the activated partial thromboplastin time (APTT) and inhibited the factor LX in the intrinsic pathway of the blood coagulation cascade. But, the fibrin plate assay did not show that the anticoagulant is a fibrinolytic protease. The molecular mass of the purified S. broughtonii anticoagulant was measured to be about 26.0kDa by gel filtration on a Sephadex G-75 column and SDS-PAGE under denaturing conditions. The optimum activity in the APTT assay was exhibited at pH 7.0-7.5 and $40-45^{\circ}C$ in the presence of $Ca^{2+}$.

• 대한본초학회지
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• 제24권2호
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• pp.7-11
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• 2009

Objectives : This research was investigated anticoagulant effect of the Chaenomelis Fructus extract. Methods : To examine an active effect of anticoagulation in Chaenomelis Fructus extract, the study measured Prothrombin time(PT) and activated partial thromboplastin time (APTT) of human plasma in vitro and measured bleeding time and arterio-venous shunt model in rats in vivo. Results : Bleeding time of Chaenomelis Fructus extract in vivo had a significant increase as about 1.6 times and thrombus weight of Chaenomelis Fructus extract had a significant reduction of thrombus weight as 50%. Chaenomelis Fructus extract represented an effect of anticoagulation by operating on extrinsic pathway factor II, V, VII, X and intrinsic pathway factor VIII, IX, X, XI, XII in the coagulation system. Conclusions : Considering the above mentioned results, it is judged that a Chaenomelis Fructus extract has a control effect of thrombus creation.

• 대한방사선기술학회지:방사선기술과학
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• 제23권1호
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• pp.13-19
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• 2000

We have presented with the "A study on overexposure rate according to over-density in chest X-ray radiography(I)" last year. In this report, We could calculate the entrance skin dose from chest X-ray film density the formula $I_0=Ix/e^{-{\mu}x}{\times}mG$, (mG is Bucky factor) was used to deliver the skin dose. At that time, There was two problems that the Bucky factor from maker was not equal to field experience and the field size influenced on the Attenuation Rate. The experiment of Bucky factor was done from film method and retried the Attenuation Rate of Acryle phantom according to Good & Poor geometry. As the results, The Bucky factor from maker higher than in this experiments $30{\sim}40%$. The Attenuation Rate in good geometric condition brings about a little alteration compare with poor geometric condition. In the field experiment, we could get the chest image with very low entrance skin radiation dose $29.3{\mu}Sv$, especially with air gap methode, the entrance skin dose was detected $10{\mu}Sv$.

• 한국콘텐츠학회논문지
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• 제22권4호
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• pp.700-705
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• 2022

혈우병은 혈액 응고에 필요한 13개의 인자들 중 1개라도 결핍될 경우 혈액 응고에 장애가 발생하여 지혈 이상을 보이는 선천성 출혈성 질환이다. 13개의 인자들 중 결핍된 응고 인자에 따라 혈우병 A(제8번 인자, factor VIII), 혈우병 B(제9번 인자, factor IX), 혈우병 C(제10번 인자, factor X)로 분류된다. 혈우병 A 진단을 받은 40대 남성 환자가 전체적인 만성 치아 주위 염증으로 인한 치조골 흡수 및 치주농양을 문제를 주소로 을지대학교병원 치과에 내원하였고, 초진당시 전반적인 골흡수 및 염증상태를 보였다. 혈액응고인자의 농도를 적절히 유지하며 관혈적 및 비관혈적 치료를 시행한 바, 10년 동안의 정기적인 내원으로 #35, #36 부위를 제외한 나머지 부위에서 별다른 합병증이나 출혈성향은 관찰되지 않았고, 전반적으로 양호한 구강 상태를 보였으며 부작용이나 합병증은 보이지 않았다.

• The Korean Journal of Physiology and Pharmacology
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• 제15권2호
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• pp.83-88
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• 2011

A large body of evidence has indicated that induction of endogenous antioxidative proteins seems to be a reasonable strategy for delaying the progression of cell injury. In our previous study, cilostazol was found to increase the expression of the antioxidant enzyme heme oxygenase-1 (HO-1) in synovial cells. Thus, the present study was undertaken to examine whether cilostazol is able to counteract tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$)-induced cell death in endothelial cells via the induction of HO-1 expression. We exposed human umbilical vein endothelial cells (HUVECs) to TNF-${\alpha}$ (50 ng/ml), with or without cilostazol ($10{\mu}M$). Pretreatment with cilostazol markedly reduced TNF-${\alpha}$-induced viability loss in the HUVECs, which was reversed by zinc protoporphyrine IX (ZnPP), an inhibitor of HO-1. Moreover, cilostazol increased HO-1 protein and mRNA expression. Cilostazol-induced HO-1 induction was markedly attenuated not only by ZnPP but also by copper-protoporphyrin IX (CuPP). In an assay measuring peroxisome proliferator-activated receptor-${\gamma}$ (PPAR-${\gamma}$) transcription activity, cilostazol directly increased PPAR-${\gamma}$ transcriptional activity which was completely abolished by HO-1 inhibitor. Furthermore, increased PPAR-${\gamma}$ activity by cilostazol and rosiglitazone was completely abolished in cells transfected with HO-1 siRNA. Taken together, these results indicate that cilostazol up-regulates HO-1 and protects cells against TNF-${\alpha}$-induced endothelial cytotoxicity via a PPAR-${\gamma}$-dependent pathway.

• Journal of Dental Anesthesia and Pain Medicine
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• 제18권6호
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• pp.333-337
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• 2018

Ramsay Hunt syndrome is a type of acute herpes zoster, which occurs by reactivation of the varicella-zoster virus at the geniculate ganglion. Clinical presentation of Ramsay Hunt syndrome includes a vesicular rash on the ear (herpes zoster oticus) or in the oral mucosa accompanied by acute peripheral facial nerve paralysis. Other cranial nerves such as V, IX, XI, and XII are often involved. Additional variability of the clinical picture of Ramsay Hunt syndrome is produced by varying patterns of skin involvement explained by individual anastomoses between cranial and cervical nerves. Combination treatment containing anti-viral agents and steroids is recommended for the treatment of Ramsay Hunt syndrome. Additionally, early diagnosis of Ramsay Hunt syndrome is a crucial factor to improve damaged nerves in Ramsay Hunt syndrome, which initiates treatment as soon as possible.

• 한국의류학회지
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• 제21권1호
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• pp.170-181
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• 1997

The purpose of this study was to determine the effect of knit structure and knit density (machine tightness factor) on the dimensional properties and K1-4 values of weft-knitted fabrics followed over eleven cycles of mechanical relaxation to provide the basic data for constructing weft-knitted fabrics for outwear with excellent dimensional stability The eighteenth weft-knitted fabrics were produced with different knit structure (1$\times$1 rib, half-cardigan rib, half-milano rib, interlock, single pique, crossmiss interlock) and machine tightness factor (loose, medium, tight) for this study. Dimensional properties such as width, lengh, area shrinkage and dimensional parameter (K) of eighteenth knitted fabrics including thickness and bulk property were measured. The results were as follows; 1. The dimensional behavior of the Ix1 rib and interlock in relaxation cycles was anisotropic, i.e., length shrinkage was usually associated with a width expansion, whereas the other weft-kntted fabrics which have tuck or miss loops in the knit structure behaved isotropically, i.e., length and width shrinkages were usually found. It was proposed that the difference in dimensional behavior between these structures was due to the dissimilar nonrelaxed geometrical shapes of the individual structural units forming these weft-knitted structures. The mechanical relaxation shrinkage of weft-knitted cotton fabrics was dependent on the tightness of construction. For a range of fabrics knitted on this study, an increase in fabric tightness caused a decrease in the length shrinkage of the fabric accompanied by an increase in its width shrinkage.