• Journal of Animal Science and Technology
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• 제64권3호
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• pp.481-499
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• 2022

This study aims to determine the effects of D-methionine (D-Met) isomer and the methionine precursor 2-hydroxy-4-methylthiobutanoic acid i (HMBi) supplementation on milk protein synthesis on immortalized bovine mammary epithelial cell (MAC-T). MAC-T cells were seeded using 10-cm dishes and cultured in Dulbecco's modified Eagle's medium/F12 (DMEM/F12) basic medium. The basic medium of DMEM/F12 was replaced with the lactogenic DMEM/ F12 differentiation medium when 90% of MAC-T cells reached confluency. The best dosage at 0.6 mM of D-Met and HMBi and incubation time at 72 h were used uniformly for all treatments. Each treatment was replicated six times wherein treatments were randomly assigned in a 6-well plate. Cell, medium, and total protein were determined using a bicinchoninic acid protein assay kit. Genes, proteomics and metabolomics analyses were also done to determine the mechanism of the milk protein synthesis pathway. Data were analyzed by two-way analysis of variance (ANOVA) with supplement type and plate as fixed effects. The least significant difference test was used to evaluate the differences among treatments. The HMBi treatment group had the highest beta-casein and S6 kinase beta-1 (S6K1) mRNA gene expression levels. HMBi and D-Met treatments have higher gene expressions compared to the control group. In terms of medium protein content, HMBi had a higher medium protein quantity than the control although not significantly different from the D-Met group. HMBi supplementation stimulated the production of eukaryotic translation initiation factor 3 subunit protein essential for protein translation initiation resulting in higher medium protein synthesis in the HMBi group than in the control group. The protein pathway analysis results showed that the D-Met group stimulated fructose-galactose metabolism, glycolysis pathway, phosphoinositide 3 kinase, and pyruvate metabolism. The HMBi group stimulated the pentose phosphate and glycolysis pathways. Metabolite analysis revealed that the D-Met treatment group increased seven metabolites and decreased uridine monophosphate (UMP) production. HMBi supplementation increased the production of three metabolites and decreased UMP and N-acetyl-L-glutamate production. Taken together, D-Met and HMBi supplementation are effective in stimulating milk protein synthesis in MAC-T cells by genes, proteins, and metabolites stimulation linked to milk protein synthesis.

• Journal of Ginseng Research
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• 제47권1호
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• pp.106-116
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• 2023

Background: Pirarubicin (THP) is an anthracycline antibiotic used to treat various malignancies in humans. The clinical usefulness of THP is unfortunately limited by its dose-related cardiotoxicity. Ginsenoside F1 (GF1) is a metabolite formed when the ginsenosides Re and Rg1 are hydrolyzed. However, the protective effects and underlying mechanisms of GF1 on THP-induced cardiotoxicity remain unclear. Methods: We investigated the anti-apoptotic and anti-oxidative stress effects of GF1 on an in vitro model, using H9c2 cells stimulated by THP, plus trigonelline or AKT inhibitor imidazoquinoxaline (IMQ), as well as an in vivo model using THP-induced cardiotoxicity in rats. Using an enzyme-linked immunosorbent test, the levels of malondialdehyde (MDA), brain natriuretic peptide (BNP), creatine kinase (CK-MB), cardiac troponin (c-TnT), lactate dehydrogenase (LDH), superoxide dismutase (SOD) and glutathione (GSH) were determined. Nuclear factor (erythroid-derived2)-like 2 (Nrf2) and the expression of Nrf2 target genes, including heme oxygenase-1 (HO-1), glutathione-S-transferase (Gst), glutamate-cysteine ligase modifier subunit (GCLM), and expression levels of AKT/Bcl-2 signaling pathway proteins were detected using Western blot analysis. Results: THP-induced myocardial histopathological damage, electrocardiogram (ECG) abnormalities, and cardiac dysfunction were reduced in vivo by GF1. GF1 also decreased MDA, BNP, CK-MB, c-TnT, and LDH levels in the serum, while raising SOD and GSH levels. GF1 boosted Nrf2 nuclear translocation and Nrf2 target gene expression, including HO-1, Gst, and GCLM. Furthermore, GF1 regulated apoptosis by activating AKT/Bcl-2 signaling pathways. Employing Nrf2 inhibitor trigonelline and AKT inhibitor IMQ revealed that GF1 lacked antioxidant and anti-apoptotic effects. Conclusion: In conclusion, GF1 was found to alleviate THP-induced cardiotoxicity via modulating Nrf2 and AKT/Bcl-2 signaling pathways, ultimately alleviating myocardial oxidative stress and apoptosis.

• 한방재활의학과학회지
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• 제33권2호
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• pp.1-18
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• 2023

Objectives The purpose of this study was to evaluate the antioxidant, anti-inflammatory and wound healing effects of Samul-tang-ga-dansam water extract (SD) in wound-induced mice. Methods The mice were divided into five groups (n=7): the normal group, the control group, the positive control group, the low-dose SD group and the high-dose SD group. The normal group had no wounds and the other groups were wounded on the back with a leather punch. Distilled water was administered to the control group, 200 mg/kg of vitamin E was administered to the positive control group. In the low-dose SD group and the high-dose SD group, 1.23 g/kg and 2.47 g/kg of SD were administered, respectively. Antioxidant and anti-inflammatory protein levels were evaluated using western blot analysis. Skin tissue was analyzed by H&E, Masson's trichrome staining method. Results Oral administration of the SD significantly reduced the visible skin damage and decreased the reactive oxygen species and ONOO^- activity of the serum. It significantly increased heme oxygenase-1, superoxide dismutase, catalase, GPx-1/2, Nrf2 and Keap-1 which are antioxidant-related factors in skin tissue and reduced NF-κB p65, inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor α, interleukin (IL)-1β, IL-6 which are inflammation-related factors. Also, SD significantly decreased NOX2, p22^phox and p47^phox and increased α-smooth muscle actin and COL1A1 protein expression in fibroblasts involved in connective tissue repair. According to histological examination, the thickened epithelial layer was thinned and collagen fibers were increased to accelerate wound healing. Conclusions It is suggested that Samul-tang-ga-dansam has antioxidant and anti-inflammatory effects and promotes wound tissue repair.

• 한국작물학회지
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• 제27권3호
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• pp.276-282
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• 1982

본 시험은 1981년에 참깨품종 "수원 9호"를 Pot에 공시하여 토양의 배수력 차이에 따른 참깨의 생육과 수량형성의 차이를 구명하고자 시도되었다. 즉 점토와 세사를 용량비(V/V Ratio)로 각각 0:100, 25:75, 50:50, 75:25 및 100:0가 되도록 혼합한 토양에 6일 간격 20mm 관수와 3일 간격 10mm 관수의 효과와 반응차이를 해석하였다. 토양의 용량비에 따른 수분의 배수성은 점토혼합율에 따라 거의 직선적으로 감소하는 경향이었다. 토양배수성 차이에 따른 참깨의 생육반응은 순세사토에서만 한해가 인정되므로 관수의 증대가 요구되었고 그 외의 배수조건의 차이에 따른 생육반응에는 통계적 유의차가 없었다. 수량구성요소 가운데서 수량에 기여도가 가장 컸던 것은 주당삭수로서 수량과 마찬가지로 점토와 사토가 75%:25%로 혼합된 조건 즉 배수성보다는 보습성이 양호한 토양에서 가장 많았으며 삭당입수나 등숙성은 선행형질인 주당삭수와의 부적상관 때문에 보습성 보다는 배수성이 양호한 토양에서 증대되는 경향을 나타내었다. 관수방법간에 이들 수량구성 특성의 차이는 없었으나 수량은 6일 간격 20mm보다 3일 간격 10mm 관수로서 보습력(점사율 75%:25%)이 높은 조건에서 최다수준이었으며 과도한 배수성이나 보습성의 조건에서는 뚜렷한 감수의 경향이었다.감수의 경향이었다.

• Nuclear Engineering and Technology
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• 제55권3호
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• pp.1061-1070
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• 2023

Over the last few years, lead-free inorganic metal perovskites have gained impressive ground in empowering satellites in space exploration owing to their material stability and performance evolution under extreme space environments. The present work has examined the versatility of eight such perovskites as space radiation shielding materials by computing their photon, charged particles and neutron interaction parameters. Photon interaction parameters were calculated for a wide energy range using PAGEX software. The ranges of heavy charged particles (H, He, C, N, O, Ne, Mg, Si and Fe ions) in these perovskites were estimated using SRIM software in the energy range 1 keV-10 GeV, and that of electrons was computed using ESTAR NIST software in the energy range 0.01 MeV-1 GeV. Further, the macroscopic fast neutron removal cross-sections were also calculated to estimate the neutron shielding efficiencies. The examined shielding parameters of the perovskites varied depending on the radiation type and energy. Among the selected perovskites, Cs₂TiI₆ and Ba₂AgIO₆ displayed superior photon attenuation properties. A 3.5 cm thick Ba₂AgIO₆-based shield could reduce the incident radiation intensity to half its initial value, a thickness even lesser than that of Pb-glass. Besides, CsSnBr₃ and La_0.8Ca_0.2Ni_0.5Ti_0.5O₃ displayed the highest and lowest range values, respectively, for all heavy charged particles. Ba₂AgIO₆ showed electron stopping power (on par with Kovar) better than that of other examined materials. Interestingly, La_0.8Ca_0.2Ni_0.5Ti_0.5O₃ demonstrated neutron removal cross-section values greater than that of standard neutron shielding materials - aluminium and polyethylene. On the whole, the present study not only demonstrates the employment prospects of eco-friendly perovskites for shielding space radiations but also suggests future prospects for research in this direction.

• 한방재활의학과학회지
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• 제33권3호
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• pp.47-65
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• 2023

Objectives We evaluated the wound healing effects of Dangguijakyak-san (DJ) using C57BL/6 mice that were generated open wound. Methods The study was conducted with seven C57BL/6 mice assigned to each group, divided into the normal group, control group, vitamin E group, DJ low-dose group, DJ high-dose group. We measured total polyphenol, flavonoid contents, the size of the wound, liver function, pro-inflammatory cytokine activity in serum, inflammation-related proteins, adhesion molecules and chemokine proteins, collagen-related proteins in skin tissue and histopathological changes by H&E and Masson's staining. Results DJ treatment significantly reduced the area of the wound compared to the control group. Also, inflammatory cytokines were reduced and the expression of anti-inflammatory-related factors (interleukin-4 [IL-4] and IL-10) was significantly increased in the DJ treatment group. We identified that DJ treatment inhibits both pathways of inflammation, the mitogen-activated protein kinases and nuclear factor-κB pathway. Moreover, the protein expressions of Sirt1 (sirtuin 1), MCP-1 (monocyte chemoattractant protein 1), ICAM-1 (intercellular adhesion molecule 1), and VCAM-1 (vascular cell adhesion molecule 1) were decreased by DJ administration. Also, the expression of α-smooth muscle actin and collagen type I alpha 1, collagen-related proteins, that help skin recovery was significantly increased in the DJ treatment group. Histopathologically, a relatively thin epithelial layer could be observed in the DJ administration group, as well as an increase in fibroblasts and collagen fibers. Conclusions These data suggest that DJ treatment is effective in wound healing, suppressing inflammatory proteins, increasing skin repair factors and improving histopathological changes caused by wounds.

• 분석과학
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• 제21권2호
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• pp.93-101
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• 2008

SPME 분석기법은 헤드스페이스(Headspace: HS) 방식으로 기체상태의 흡착을 유도하거나, 액상시료에 직접 접촉하는 방식으로 분석대상 물질을 추출할 수 있다. 본 연구에서는 CAR/PDMS fiber를 이용하여, 액상시료의 VOC 분석을 시도하였다. 3 가지 변수 (흡착온도, 흡착시간, 교반여부)의 강약을 동시에 감안한 8가지 조합조건에서 분석효율을 조사하였다. (1) 용출온도: 30 대비 $50^{\circ}C$. (2) SPME fiber 시료노출시간: 10과 30 min. (3) 교반적용 여부: 무 교반 대비 1200 rpm. 8가지의 조합형 분석조건에서 HS-SPME 방식을 적용하여분석한 결과, S50-30 (stirring speed: 1200 rpm, exposure temp: 50oC, exposure time: 30 min)에서 가장 분석효율이 높게 나타났다. 가장 좋은 감도를 보인 S50-30방식의 분석 회수율을 GC에 직접주입하는 방식을 임의의 기준으로 평가하였을 때, 성분에 따라 상대 회수율이 45.5~68.5%로 나타났다. 본 연구의 결과, 3가지 변수 중에서 교반여부는 검량 특성을 결정짓는 가장 중요한 인자로 나타났다.

• 대한토목학회논문집
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• 제28권2A호
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• pp.281-288
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• 2008

콘크리트와 강재의 특성을 살린 복합구조 교량에 있어서 새로운 구조 형식인 복부 파형강판 PSC 박스거더 교량은 시공성 및 경제적인 측면에서 우수한 장점이 있다. 그러나 이와 관련된 기존 연구는 전단 좌굴에 대한 복부 파형강판의 거동 및 접합부의 형상 개발과 관련하여 한정된 측면이 있으며, 교량 자체로서의 비틀림 거동에 대한 연구는 다소 부족한 실정으로 합리적인 비틀림 해석모델의 개발과 거동 예측에 대한 연구가 필요하다고 판단된다. 본 연구에서는 복부 파형강판 PSC 박스거더 교량에 대하여, 공간트러스 모델을 이용한 Rausch의 제안식과 RC 요소의 연화효과가 고려된 평형 방정식, 적합 조건식을 활용하고 복부 파형강판 구조 형태에 의하여 추가적으로 고려해야 할 구성 방정식과 모델, 제안식 등을 적용한 비틀림 해석모델을 제시하고 기존에 수행된 부재 실험결과와의 비교를 통하여 해석모델의 합리성을 검토하였다. 또한 복부 파형강판 PSC 박스의 비틀림 거동에 영향을 미치는 프리스트레싱 힘과 콘크리트 압축강도를 변수로 한 해석을 진행하였으며, 제안된 비틀림 해석모델을 이용하여 복부 파형 강판과 프리스트레스 된 콘크리트 부재의 단면 형상비(H/B)에 따른 해석을 수행하여 보다 합리적인 비틀림 정수와 이와 관련한 수정식을 제안하였다.

• 한국식생활문화학회지
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• 제22권2호
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• pp.176-184
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• 2007

The purpose of this dissertation is to analyze the factors which university students feel as stress in their daily life and the correlation between these and their eating habits. The subject are students enrolled at 'H' university in Asan. There are 801 students who response for this research. We surveyed and analyzed the correlation between the life stress and eating habit of university students. It is analyzed that the objects suffered stress most by 'schooling' and 'job complication.' Surveying the general eating habit of university students, it is found that they do not eat meal regularly, i.e., 'average meals (40.9%)' and 'irregular meal(35.2%).' No significant difference was observed in the regularity of meals between sexes. When it comes to overeating or voracity, male students answered 'sometimes(77.6%)' or 'often(22.5%)', but female students answered 'sometimes(66.2%)' or 'often(28.2%)', which means that female students are more frequent in overeating or voracity. The frequency of eating snack is more than once a day 60.1% in female students and 47.3% in male students, which shows that the rate of female students is much higher than that of male students. Surveying the correlation between stress that male and female university students suffer and the regular meal, we found that both of them tend to eat meal irregularly if they are stressed. Comparing the stress for its factor, male students eat meal irregularly if they are stressed by 'schooling' and female students eat meal irregularly if they are stressed by 'figure.' Checking the correlation between stress and time spent for meal, it is found that while male students eat meal in a hurry when they are stressed by 'job affair', female students do so when they are stressed by 'home economy. Analyzing the correlation between frequency of snack and stress, both male and female tend to eat snack more frequently when they are stressed more. In general, males like the oily food more than male, but when students get stresses, females want to eat oily food than male. Analyzing the correlation between stress and preferential menu, males like green vegetables, sea food in the life get less stresses than others Females like a menu of meat get higher stress than others.

• 운동과학
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• 제21권3호
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• pp.289-298
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• 2012

본 연구는 당뇨를 유발한 흰쥐에서 트레드밀 운동이 망막 신경세포의 사멸에 억제 효과가 있는지를 실험 하였다. 본 연구에서 Sprague-Dawley계 흰쥐 28마리를 대조군, 운동군, 당뇨군, 당뇨운동군으로 분류하여 각 군당 7마리씩 배정하였다. 당뇨는 streptozotocin을 복강에 주사하여 유발하였다. 운동군은 분당 8 m의 속도로 하루 30분씩 주 5회, 총 12주 동안 트레드밀 운동을 실시하였다. 본 연구의 결과, 당뇨 유발 흰쥐의 망막에서 세포사멸 인자인 terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL)-양성 세포수 그리고 caspase-3, cytochrome c, Bax의 발현이 증가되었으며, 항 세포사멸 인자인 Bcl-2의 발현은 감소되었다. 트레드밀 운동은 TUNEL-양성 세포수 그리고 caspase-3, cytochrome c, Bax의 발현을 감소시켰으며, Bcl-2의 발현은 증가시켰다. 본 실험의 결과, 당뇨에 의한 망막의 세포사멸 증가에 트레드밀 운동이 억제 작용을 나타내었으며, 따라서 트레드밀 운동은 당뇨 환자들에서 후유증을 경감시키는데 효과적인 치료법임을 알 수 있었다.