• Journal of Microbiology and Biotechnology
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• v.25 no.1
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• pp.119-126
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• 2015

Among the various human growth factors, epidermal growth factor (hEGF, consisting of 53 amino acids) has various effects on cell regeneration, stimulation of proliferation, migration of keratinocytes, formation of granulation tissues, and stimulation of fibroblast motility, which are important for wound healing. Owing to their multiple activities, EGFs are used as pharmaceutical and cosmetic agents. However, their low productivity, limited target specificity, and short half-life inhibit their application as therapeutic agents. To overcome these obstacles, we fused the collagen-binding domain (CBD) of Vibrio mimicus metalloprotease to EGF protein. About 18 or 12 amino acids (aa) (of the 33 total amino acids), which were essential for collagen-binding activity, were combined with the N- and C-termini of EGF. We constructed, expressed, and purified EGF (53 aa)-CBD (18 aa), EGF (53 aa)-CBD (12 aa), CBD (18 aa)-EGF (53 aa), and CBD (12 aa)-EGF (53 aa). These purified recombinant proteins increased the numbers of cells in treated specimens compared with non-treated specimens and control hEGF samples. The collagen-binding activities were also evaluated. Furthermore, CBD-hybridized hEGF induced phosphorylation of the EGF receptor. These results suggested that these fusion proteins could be applicable as small therapeutic agents in wound tissue healing.

• The Journal of Korean Obstetrics and Gynecology
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• v.24 no.3
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• pp.146-155
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• 2011

Objectives: Since early developed measurement of Postpartum depression(PPD) doesn't reflect physical symptoms, we need to find out how PPD and related physical disorders are expressed in functional medical methods. The study was conducted to find out characteristics of 7 Zone diagnosis and postpartum physical symptoms on PPD. Methods: To 216 postpartum women who did normal delivery, we measured 7-zone-diagnosis Factor AA's height on 5th day and 15th day postpartum. And we surveyed EPDS and postpartum physical symptoms question on 15th day postpartum. Subjects were divided into normal and depression group by EPDS score. And we compared Factor AA's height between two groups and Factor AA's height change in each group. Results: In depression group, Factor AA's height was higher than normal group on 5th day and 15th day postpartum. In both groups Factor AA's height was decreased on 15th day compared to 5th day postpartum. In normal group 2, 6, 7 zone were significantly decreased and in depression group only 6 zone was significantly decreased. Conclusions: These results demonstrate that postpartum depression group has low recuperative power. And these can be judged by Factor AA's height change.

• Journal of Acupuncture Research
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• v.24 no.6
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• pp.159-170
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• 2007

Objectives : The 7-zone-diagnostic system is a diagnostic device to predetermine bodily locations by measuring the energy of a living body. This study was to investigate the relation between the different patterns of Factor AA in a 7-zone-diagnostic system and clinical parameters. The purpose of this study is to relate Korean traditional medicine and western medicine using the data from the 7-zone-diagnostic system and the clinical parameters. Methods : This study was carried out with the data from some clinical parameters. We made two groups according to the Factor AA patterns of VEGA-DFM 722, the 7-zone-diagnostic system The Factor AA patterns of Group A named hyperenergy is all the red bar graphs that arehigher than the normal range. The Factor AA patterns of Group B named hypoenergy is all the red bar graphs that are lower than the normal range. After the data from clinical parameters corresponding with conditions of each group were selected, the data from clinical parameters among each group was analyzed statistically. Results : The values of Weight, GOT, r-GTP, Uric acid and BMI of Group A are higher than those of Group B. The values of Sodium and Phosphorus of Group A are lower than those of Group B. Conclusions : To conclude, it is thought that Group A has a heat-excess type but Group B has colddeficient type.

• Journal of Acupuncture Research
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• v.25 no.6
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• pp.67-76
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• 2008

Objectives : The 7-zone-diagnostic system is a diagnostic device to predetermine bodily locations by measuring the energy of body. This study was to investigate the relation between the different patterns of Zone 1, 2, 3 of Factor AA in CP-6000A(VEGA, Germany), 7-zone-diagnostic system and clinical parameters. The purpose of this study was relation Korean traditional medicine and western medicine with the data from 7-zone-diagnostic system and the clinical parameters. Methods : This study was carried out with the data from some clinical parameters. We made three groups according to the Factor AA patterns of CP-6000A. The Factor AA pattern of Group A is that the red bar graph of zone 1, 2, 3 were higher than the normal range and the others were the normal range. The Factor AA pattern of Group B was that the red bar graph of zone 1, 2, 3 was the normal range and the others were the normal range. The Factor AA pattern of Group C was that the red bar graph of zone 1, 2, 3 was lower than the normal range and the others were the normal range. After the data from clinical parameters to correspond with conditions of each group were selected, the data from clinical parameters among each groups analyzed statistically. Results : The values of GOT, GPT, r-GPT, Triglyceride, BUN, Uric acid of group A was higher than group C. Gastroscope of group A and B was higher than group C. Conclusions : It is thought that the red bar graph of zone 1, 2, 3 is higher, the group has the higher energy and the energy has a character of fire(熱). Those patterns have a high risk of hyperlipermia and liver, stomach disease.

• Journal of Acupuncture Research
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• v.25 no.4
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• pp.71-80
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• 2008

Objectives : The 7-zonediagnostic system is a diagnostic device to predetermine bodily locations by measuring the energy of body. This study was to investigate the relation between the different patterns of Zone 4 of Factor AA in VEGA DFM 722 (VEGA, Germany), 7-zone-diagnositic system and heart rate variability. Methods : We made three groups according to the Factor AA patterns of VEGA DFM 722. The Factor AA pattern of Group A is that the red bar graph of zone 4 was higher than the normal range. The Factor AA patterns of Group B was that the red bar graph of zone 4 was located at the normal range. The Factor AA patterns of Group C was that the red bar graph of zone 4 was lower than the normal range. We investigated how to difference of the index of heart rate variability(HRV, LX-3202, LAXTHA, Korea) according to each groups. Results : Complexity, HRV-index, RMSSD, SDSD values of Group B were higher than other Groups. pNN50 values of Group B were lower than other groups. And Ln(TP), Ln(VLF), Ln(LF), Ln(HF) values of Group B were higher than other groups. Conclusions : We presumed that Group B was healthier than other groups for the stress.

• The Bulletin of The Korean Astronomical Society
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• v.41 no.1
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• pp.79.3-80
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• 2016

We have investigated the correlation analysis between global temperature anomaly and two main factors: geomagnetic activity (aa index) of Earth external factor and CO2 of Earth internal factor. For this, we used NOAA Global Surface Temperature anomaly (Ta) data from 1868 to 2015. The aa index indicates the geomagnetic activity measured at two anti-podal subauroral stations (Canberra Australia and Hartland England) and the CO2 data come from historical ice core records and NOAA/ESRL data. From the comparison between (Ta) and aa index, we found several interesting things, First, the linear correlation coefficient between two parameters increases until 1985 and then decreases rapidly. Second, the scattered plot between two parameters shows a boundary of the correlation tendency (positive and negative correlation) near 1985. A partial correlation of (Ta) and two main factors (aa index, CO2) also shows that the geomagnetic effect (aa index) is dominant until about 1985 and the CO2 effect becomes much more important after then. These results indicate that the CO2 effect become very an important factor since at least 1985. For a further analysis, we simply assume that Ta = Ta(aa)+Ta(CO2) and made a linear regression between (Ta) and aa index from 1868 to 2015. A linear model is then made from the linear regression between energy consumption (a proxy of CO2 effect) and Ta-Ta(aa) since 1985. Our results will be discussed in view of the prediction of global warming.

• Journal of Acupuncture Research
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• v.25 no.2
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• pp.139-149
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• 2008

Objectives : The 7-zone-diagnostic system is a diagnostic device to predetermine bodily locations by measuring the energy of body. This study was to investigate the relation between the different patterns of Zone 2, 6 of Factor AA in VEGA DFM 722(VEGA, Germany), 7-zone-diagnostic system and clinical parameters. The purpose of this study was relation Korean traditional medicine and western medicine with the data from 7-zone-diagnostic system and the clinical parameters. Methods : This study was carried out with the data from some clinical parameters. We made two groups according to the Factor AA patterns of VEGA DFM 722. The Factor AA pattern of Group A is that the red bar graph of zone 2 was higher than the normal range and the red bar graph of zone 6 was lower than the normal range. The Factor AA pattern of Group B was that the red bar graph of zone 2 was lower than the normal range and the red bar graph of zone 6 was higher than the normal range. After the data from clinical parameters to correspond with conditions of each group were selected, the data from clinical parameters between each gropus analyzed statistically. Results and Conclusions : The values of Direct Bilirubin, GOT, BUN and BUN/Creatinine ratio of Group A were higher than them of Group B. The values of Sodium and Tyroxine of Group A were lower than them of Group B. These results mean that Group A has low energy but has increading tendency. To conclude, it is thought that the red bar graph of zone 2 is higher, the group is the more increasing and the red bar graph of zone 6 is lower, the group has the lower energy.

• The Korea Journal of Herbology
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• v.28 no.6
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• pp.129-133
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• 2013

Objectives : The purpose of this study was to investigate the effects of Angelicae acutilobae Radix Water Extract (AA) on the production of cytokines in RAW 264.7 cell stimulated with lipopolysaccharide (LPS). Method : RAW 264.7 cells were cotreated with AA (50 and $100{\mu}g/mL$) and lipopolysaccharide (LPS; $1{\mu}g/mL$) for 24 hours. After 24 hours treatment, using bead-based multiplex cytokine assay, concentrations of various cytokines such as interleukin(IL)-6, tumor necrosis factor-alpha(TNF-${\alpha}$) granulocyte colony-stimulating factor(G-CSF), granulocyte macrophage colony-stimulating factor(GM-CSF), and macrophage inflammatory protein(MIP)-$1{\alpha}$ were measured. Result : AA significantly inhibited LPS-induced production of IL-6 and MIP-$1{\alpha}$ from LPS-stimulated RAW 264.7 cells at the concentration of $50{\mu}g/mL$. AA significantly inhibited LPS-induced production of TNF-${\alpha}$ from LPS-stimulated RAW 264.7 cells at the concentration of $100{\mu}g/mL$. AA significantly inhibited LPS-induced production of G-CSF and GM-CSF in RAW 264.7 cells at the concentrations of 50 and $100{\mu}g/mL$. Conclusion : These results suggest that AA has anti-inflammatory effect related with its inhibition of proinflammatory cytokines such as IL-6, TNF-${\alpha}$, G-CSF, GM-CSF, and MIP-$1{\alpha}$ in LPS-induced macrophages.

• International Journal of Oral Biology
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• v.45 no.4
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• pp.169-178
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• 2020

L-ascorbic acid (L-AA; vitamin C) induces apoptosis in cancer cells. This study aimed to elucidate the molecular mechanisms of L-AA-induced apoptosis in human laryngeal epidermoid carcinoma Hep-2 cells. L-AA suppressed the viability of Hep-2 cells and induced apoptosis, as shown by the cleavage and condensation of nuclear chromatin and increased number of Annexin V-positive cells. L-AA decreased Bcl-2 protein expression but upregulated Bax protein levels. In addition, cytochrome c release from the mitochondria into the cytosol and activation of caspase-9, -8, and -3 were enhanced by L-AA treatment. Furthermore, apoptosis-inducing factor (AIF) and endonuclease G (EndoG) were translocated into the nucleus during apoptosis of L-AA-treated Hep-2 cells. L-AA effectively inhibited the constitutive nuclear factor-κB (NF-κB) activation and attenuated the nuclear expression of the p65 subunit of NF-κB. Interestingly, L-AA treatment of Hep-2 cells markedly activated Akt and mitogen-activated protein kinase (MAPK; extracellular signal-regulated kinase 1/2, p38, and c-Jun N-terminal kinase [JNK]) and and LY294002 (Akt inhibitor), SB203580 (p38 inhibitor) or SP600125 (a JNK inhibitor) decreased the levels of Annexin V-positive cells. These results suggested that L-AA induces the apoptosis of Hep-2 cells via the nuclear translocation of AIF and EndoG by modulating the Bcl-2 family and MAPK/Akt signaling pathways.

• Journal of Acupuncture Research
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• v.27 no.2
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• pp.1-10
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• 2010

Objectives : The 7-zone-diagnostic system is a diagnostic device to predetermine bodily locations by measuring the energy of body. This study was to investigate the relation between the different patterns of Zone 2 of Factor AA in CP-6000A (VEGA, Germany), 7-zone-diagnostic system and the factors of Bell's Palsy. Methods : This study was carried out with the data from factors of Bell's Palsy(age, sex, period after onset, postauricular pain, season which the disease happen, contributing factor). We made three groups according to the different patterns of Zone 2 of Factor AA in CP-6000A. The Factor AA pattern of Group A is that the red bar graph of zone 2 were lower than the normal range. The Factor AA pattern of Group B was that the red bar graph of zone 2 was the normal range. The Factor AA pattern of Group C was that the red bar graph of zone 2 was higher than the normal range. After collecting the data about factors of Bel's Palsy to correspond with conditions of each group were selected, the data was analyzed statistically. Results : The values of number of patient and period after onset of group A was higher than group B and C. The values of ratio of postauricular pain of group B and C was higher than group A. The season of disease happened of group A is spring and winter, group B is winter and group C is summer and autumn. The contributing factor of disease happened of group A is none, and group B and C is stress and clod. Conclusions : In Bell's Palsy, it is thought that the red bar graph of zone 2 is lower, the group have lower energy(虛) and the energy has a character of cold(寒), and zone 2 is higher, the group has the higher energy(實) and the energy has a character of fire(熱).