• Title/Summary/Keyword: Factions

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Antioxidant Flavonoids and Chlorogenic Acid from the Leaves of Erobotrya japonica

  • Jung, Hyun-Ah;Park, Jong-Cheol;Chung, Hae-Young;Kim, Jong;Choi, Jae-Sue
    • Archives of Pharmacal Research
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    • v.22 no.2
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    • pp.213-218
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    • 1999
  • The antioxidant activity of Eriobotrya japonica was determined by measuring the radical scavenging effect on DPPH (1,1-diphenyl-2-picrylhydrazyl) radical and lipid peroxidation produced when mouse liver homogenate was exposed to the air at $37^{\circ}C$, using 2-thiobarbituric acid (TBA). The methanol extract and its factions of Eriobotrya japonica leaves showed strong antioxidant activity. The antioxidant activity of EtOAc and n-BuOH soluble fractions were stronger than the others, and were further purified by repeated silica gel, MCl gel CHP-20P, and Sephadex LH-20 column chromatography. Antioxidant chlorogenic acid, quercetin-3-sambubioside from n-BuOH fraction and methyl chlorogenate, kaempferol- and quercetin-3-rhamnosides, together with the inactive ursolic acid and$ 2{\alpha}$-hydroxyursolic acid from EtOAc fraction were isolated. Antioxidant flavonoids and chlorogenic acid also showed prominent inhibitory activity against free radical generation in dichlorofluorescein (DCF) method.

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Characteristics of Inorganic Phosphorus Fractions Accrued from the Application of paper Mill Sludge Compost in Naked Soil(I) (제지슬러지퇴비를 시용한 나지 토양의 무기태인산의 특성변화(I))

  • Lim, Hyun-Taek;Chang, Ki-Woon
    • Journal of the Korea Organic Resources Recycling Association
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    • v.8 no.3
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    • pp.104-111
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    • 2000
  • The study war carried out to evaluate the characteristics of inorganic phosphate factions in soil mixed with paper mill sludge compost(PMSC). The experiments were conducted by an incubation test. For the incubation test, application rates of PMSC to soil were 0, 2, 8, and 16%. Water content of the samples mixed with PMSC were adjusted to 10% and 15% and incubating temperatures of the samples were kept at $4^{\circ}C$ and $30^{\circ}C$, respectively. The results can be concluded as follows; pH increased depending on the higher rates of PMSC application to the soil during the incubation tests, because calcium content was $4.9cmol_c/kg$ and contents of nutrients were high. When the application rates of PMSC were increased Saloid-P and Fe-P decreased, while Al-P and Ca-P in soil increased during the incubation tests.

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Structure and Isolation of Antioxidative Substance Derived from Phaeodactylum tricornutum (Phaeodactylum tricornutum으로부터 항산화성 물질의 분리 및 구조)

  • KIM Se-Kwon;BYUN Hee-Guk;BAEK Ho-Chul;PARK Pyo-Jam;KANG Ok-Ju;KIM Jong-Bae
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.34 no.5
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    • pp.556-562
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    • 2001
  • The antioxidative activity of marine microalgae, Phaeodactylum tricornutum (P. tricornutum) of Bacillariophyceae, was determined by measuring radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. The chloroform fraction of P. tricornutum showed strong antioxidative activity, The potential antioxidative activity of factions extracted with mixture solution of organic solvents was detected in dichloromethane : methanol (2 : 1) fraction, This fraction was further purified by preparative thin layer chromatography (PTLC) and repeated reverse-phase HPLC. On the basis of chemical and spectoscopic evidence from results obtained by UV, FT-IR, EIMS and NMR, the compound purified from P. tricornutum was identified as zeaxanthin.

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Determinants of Movie Success from Foreign Countries in Korea (한국 개봉 미국 제외 외국 영화 흥행 결정 요인)

  • Wang, Bing Xin;Chon, Bum Soo
    • The Journal of the Korea Contents Association
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    • v.16 no.2
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    • pp.96-105
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    • 2016
  • This study examined determinants of movie success from foreign countries except the U.S. in Korea. This research is based on analyses of data based on 500 movies between 2004 and 2013. The major results are following; firstly, in terms of movie success, there were significant differences among three attributes including the origin of countries, genres and distributors. Although determinants of movie success from Japan were animation genre and major distributors, they were action genre and major distributors for European countries. Determinants of movie success from other countries was only animation genre. However, there were no any explanation factions for Chinese movies.

A study on problem solution method for works improvement and advancement quality of life on Job analysis of Emergency Medical Technicians (응급구조사의 직무분석에 따른 업무개선과 삶의 질 향상을 위한 문제점 해결 방법에 관한 연구)

  • Kim, Jae-Gab;Koh, Jae-Moon;Kim, Kyung-Wan;Jung, Young-Tae
    • The Korean Journal of Emergency Medical Services
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    • v.11 no.1
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    • pp.73-80
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    • 2007
  • This study is a descriptive survey research to know the levels of job satisfaction and influential factors for emergency medical technicians(EMT). This study sampled Level 1 and Level 2 Rescue Members from fire stations and EMT from general hospitals in the Gwangju City and JeollaNamdo area. Interviews for the 75 participants of this convenience sample were conducted for a two month period from July to September 2006. Data was collected on interviewing questionnaires. When looking at the results above internal factions were most influential towards satisfaction and self-responsibility, fulfillment, displays of Aptitude were highest for satisfaction. However, organizational factors were the lowest. There is a need for reform in promotion term periods and policy as well as promotion opportunities. Influential factors for job satisfaction were Role Performance Ability, Intentions for Changing Jobs, and Job Selection Motivation. Therefore, the more insufficient your ability to perform your job the less satisfied you are with it and the more fear you feel from experiences on the job the less satisfied you are with your job. Henceforth, there is a need to reform the educational programs offered to EMT for job training emphasizing job performance ability and the ability to judge for oneself on emergency situations, and reform the promotional system. Finally, there is a need for psychological consultation to offer stability to those after treating an emergency situation and encouragement for personal religion.

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Effect of Bovine Colostrum Factions on the Proliferation of Mouse Splenocytes (초유 유청 분획의 Mouse Splenocyte 증식 효과)

  • Ha Woel-Kyu;Won Do-Hee;Yang Hee-Jin;Hwang Kyung-A;Lee Soo-Won
    • Food Science of Animal Resources
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    • v.25 no.2
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    • pp.250-256
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    • 2005
  • To investigate the effect of bovine colostral whey fractions on in vitro proliferation of mouse splenocytes, polypeptide fractions were separated from acid whey into 3 fractions depending on molecular weight by ultrafiltration: Fraction I, which contains the polypeptide larger than 10,000 Da, Fraction n, which contains the polypeptide ranging from 1,000 Da to 10,000 Da and Fraction III, which contains the polypeptide smaller than 1,000 Da. Fraction II showed the highest proliferative effect of mouse splenocytes among the colostral whey fractions and this proliferative activity increased in dose dependent manner. Unheated Fraction II and Fraction III showed significantly (p<0.01) higher proliferative effects than others but heated Fraction II showed the highest enhancing effect of mouse splenocyte among heated whey fractions (p<0.01). The supplementation of Fraction II and Fraction m showed greater proliferative effect of mouse splenocytes stimulated by concanavalin A (Con A) than that of whole whey or Fraction L Proliferative effect of mouse splenocytes stimulated by phytohemagglutinin (PHA) was the highest when Fraction II was supplemented Proliferative effect of the colostral whey fractions on mouse splenocytes by stimulation of lipopolysaccharide (LPS) was markedly enhanced by supplementation of Fraction II and Fraction m compared with whole whey and Fraction L It was estimated that colostral whey fraction containing IGF-I positively affected proliferation of mouse splenocyte.

Screening the Antibacterial Activities of Streptomyces Extracts against Phytopathogens Xanthomonas oryzae pathovar oryzae, Xanthomonas campestris pathovar vesicatoria, and Pectobacterium carotovorum pathovar carotovorum

  • Kim, Seung-Hwan;Cheng, Jinhua;Yang, Seung Hwan;Suh, Joo-Won;Song, Eun-Sung;Kang, Lin-Woo;Kim, Jeong-Gu
    • Journal of Applied Biological Chemistry
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    • v.58 no.3
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    • pp.253-258
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    • 2015
  • Xanthomonas oryzae pv. oryzae (Xoo), X. campestris pv. vesicatoria (Xcv), and Pectobacterium carotovorum pv. carotovorum (Pcc) are the causative agents of bacterial blight in rice, bacterial spot in pepper, and bacterial soft rot in carrot and cabbage, respectively. To isolate novel microbial extracts with antimicrobial activities against these bacteria, approximately 5,300 different Streptomyces extracts were prepared and tested. Microbial cultures from various Streptomyces strains isolated from the Jeju Island, Baekam, Mankyoung river, Jiri mountain etc. in Korea were extracted into three different factions -secreted hydrophobic, secreted hydrophilic, and mycelia- using ethyl acetate, water, and methanol. Initially, 34, 29, and 10 extracts were selected as having antibacterial activities against Xoo, Xcv, and Pcc, respectively. Extracts 1169G4, 1172E9, and 1172E10 had the highest growth inhibition activities against both Xoo and Xcv, and extracts 1151H7 and 1152H7 showed the highest growth inhibition activities against Pcc.

Study of Nutrient Untake, Blood Lipids, and Obesity in Non Insulin Dependent Diabetes Mellitus Male Individuals (인슐린 비의존형 당뇨병 남성 환자의 영양소 섭취량, 혈중 지질 및 비만도에 관한 연구)

  • 최미자;김미경
    • Journal of the East Asian Society of Dietary Life
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    • v.4 no.2
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    • pp.17-26
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    • 1994
  • This study was an attempt to investigate various factions, such as the calorie, nutrient intake, physical activity, blood lipids, obesity prevalence and body fat distribution on NIDDM male diabetics. General characteristics, physical activity and exercise levels of subjects were invesigated by interviewing, daily calorie and nutrient intake were measured by convenient method. The following anthropometric measurements were made on all participats : weight, height. Also waist and hip circumference were measured on 174 male diabetics to get waist-to-hip circumference ratio as index of the body fat distribution. For measurement of plasma lipids, 12-hour fasting blood samples were drawn The results of this study were summarized as follows : 1. At the onset of diabetes, the major self-diagnosed symptoms were polydipsia, fatigue, and body weight redution 2. The average of daily energy intake of male diabetics was 2106 Kcal which is 96% of the RDA Percentage of energy is that carbohydrate:protein : pat=70:14:16. 3. Among the NIDDM male subjects, 59% was exercise regularly. 4. Obese subjects above in the ideal body weight of 120% are presently 17%, but 39% of subjects were reported to be obese in the past. The mean BMI of the male NIDDM diabetics is 23.3${\pm}$2.6 and the past mean BMI was 25.2${\pm}$2.7 The mean WHR was 0.93${\pm}$0.10. 5. When diabetics were divided into obese and nonobese group according to RBW, energy intake, blood pressure, blood glucose and total cholesterol were not significantly different between the two groups, but LDL and VLDL-cholesterol were significantly higher in the obese group. 6. RBW did not correlate with cholesterol and triglyceride, but WHR correlated significantly with cholesterol and triglyceride. In conclusion, these results from a present study support previous findings indicating that not only the degree of obesity but also the localization of fat is a risk factor for diabetes.

Phenolic Acid Contents and ROS Scavenging Activity of Dandelion(Taraxacum officinale) (민들레(Taraxacum officinale) 잎과 뿌리의 페놀산 조성 및 활성산소 소거활성)

  • Kim, Young-Chan;Rho, Jeong-Hae;Kim, Kyung-Tack;Cho, Chang-Won;Rhee, Young-Kyung;Choi, Ung-Kyu
    • Food Science and Preservation
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    • v.15 no.3
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    • pp.325-331
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    • 2008
  • The propose of this study was to investigate the antioxidant activity of 80% ethanol extracts and various solvent fractions of dandelion (Taraxacmn officinale) leaves and roots, Total phenolics and phenolic acid contents were also examined. The total phenol content of leaves and roots were $7.9{\pm}0.4%$ and $9.4{\pm}0.3%$ respectively. Eight phenolic acids were separated by GC, among which caffeic acid (113.7 mg%)and m-coumaric acid (152.6 mg) were the dominant phenolic acids in leaves and roots, respectively. Amongst solvent functions of leaves and roots, the ethyl acetate fraction showed the strongest radical scavenging activity. A strong correlation was found between total phenol content and electron-donating ability, and ABTS radical scavenging activity showed a similar trend as electron-donating ability. Hydroxyl-radical-scavenging activity and lipid peroxidation were significantly higher in the ethyl acetate fraction than other factions. In particular, the SOD-like activity was highest (43.6%) in the ethyl acetate fraction of dandelion leaves, and was higher than that of trolox. Thus, the ethyl acetate fraction of dandelion leaves exhibited significant phenol content, antioxidant activity, and free-radical-scavenging effects.

Degradation of [$^{14}C$]Carbofuran in Soils and Characterization of its Nonextractable Residues (토양중 [$^{14}C$Carbofuran의 분해 및 비추출성 잔류분의 특성)

  • Park, Chang-Kyu;Lee, Young-Deuk
    • Applied Biological Chemistry
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    • v.38 no.3
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    • pp.263-268
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    • 1995
  • A study was undertaken to compare degradation patterns of carbofuran in soils between submerged and upland moisture conditions [$3-^{14}C$]Carbofuran was treated in each soils at the rate of 1.0 mg/kg (87.8 kBq $^{14}C/50g$ soil) and the time-course analysis for distribution of radioactivity and degradation products were conducted. Differences in the pathway and rate of carbofuran degradation in soils were observed between submerged and upland moisture conditiona major degradation being hydrolysis at 7-C position and oxidation at 3-C position, respectively. Carbofuran showed less persistence in soils of higher moisture contents A significant portion, $24{\sim}39%$ of the total radioactivity, resided in soils as nonextractable residues at 60 days after treatment The nonextractable radioactivity was mainly located in soil organic matter, fulvic acid, humic acid and humin factions Gel filtration chromatography confirmed the incorporation of carbofuran and its degradation products into the organic matter.

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