Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Effect of Bovine Colostrum Factions on the Proliferation of Mouse Splenocytes

초유 유청 분획의 Mouse Splenocyte 증식 효과

  • Ha Woel-Kyu (Food Research & Development Lab., Pasteur Milk Co., Ltd.) ;
  • Won Do-Hee (Department of Food Biotechnology, Sungkyunkwan University) ;
  • Yang Hee-Jin (Department of Food Biotechnology, Sungkyunkwan University) ;
  • Hwang Kyung-A (Department of Food Biotechnology, Sungkyunkwan University) ;
  • Lee Soo-Won (Department of Food Biotechnology, Sungkyunkwan University)
  • 하월규 (파스퇴르유업㈜ 식품연구소) ;
  • 원도희 (성균관대학교 식품생명공학과) ;
  • 양희진 (성균관대학교 식품생명공학과) ;
  • 황경아 (성균관대학교 식품생명공학과) ;
  • 이수원 (성균관대학교 식품생명공학과)
  • Published : 2005.06.01
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Abstract

To investigate the effect of bovine colostral whey fractions on in vitro proliferation of mouse splenocytes, polypeptide fractions were separated from acid whey into 3 fractions depending on molecular weight by ultrafiltration: Fraction I, which contains the polypeptide larger than 10,000 Da, Fraction n, which contains the polypeptide ranging from 1,000 Da to 10,000 Da and Fraction III, which contains the polypeptide smaller than 1,000 Da. Fraction II showed the highest proliferative effect of mouse splenocytes among the colostral whey fractions and this proliferative activity increased in dose dependent manner. Unheated Fraction II and Fraction III showed significantly (p<0.01) higher proliferative effects than others but heated Fraction II showed the highest enhancing effect of mouse splenocyte among heated whey fractions (p<0.01). The supplementation of Fraction II and Fraction m showed greater proliferative effect of mouse splenocytes stimulated by concanavalin A (Con A) than that of whole whey or Fraction L Proliferative effect of mouse splenocytes stimulated by phytohemagglutinin (PHA) was the highest when Fraction II was supplemented Proliferative effect of the colostral whey fractions on mouse splenocytes by stimulation of lipopolysaccharide (LPS) was markedly enhanced by supplementation of Fraction II and Fraction m compared with whole whey and Fraction L It was estimated that colostral whey fraction containing IGF-I positively affected proliferation of mouse splenocyte.

Keywords

References

  1. Baumrucker, C. R. and Blum, J W. (1993) Insulin-like growth factor-I(IGF-I) in milk and dietary effect upon the neonate. In: Progress in endocrinology. Mornex, R., Jaffinol, N., and Leclere, J. (eds). The proceeding of the 9th international congress of endocrinology, Nice 1992. Parthenon Publishing Group, London, pp. 435-444
  2. Binz, K. P., Joller, P., Froesch, P., Binz, H., Zapf, J., and Froesch, E. R. (1990) Repopulation of the atrophied thymus in diabetic rats by insulin-like growth factor I. Proc. Natl. Acad. Sci. USA 87, 3690-3694 https://doi.org/10.1073/pnas.87.10.3690
  3. Clark, R., Strasser, J., McCabe, S., Robbins, K., and Jardieu, P. (1993) Insulin-like growth factor-I stimulation of lymphopoiesis. J. Clin. Invest. 92, 540-548 https://doi.org/10.1172/JCI116621
  4. Donovan, S. M. and Odle, J.(1994) Growth factors in milk as mediators of infant development. Ann. Rev. Nutr. 14, 147-167 https://doi.org/10.1146/annurev.nu.14.070194.001051
  5. Dvorak, B., Williams, C. S., McWilliam, D. L., Shinohara, H., Dominguez, J. A., McCuskey, R. S., Philipps, A. F., and Koldovsky, O. (2000) Milk-borne epidermal growth factor modulates intestinal transforming growth factor-a levels in neonatal rats. Pediatr. Res. 47, 194-200 https://doi.org/10.1203/00006450-200002000-00008
  6. Frankie, B. S. and Kitabchi, A. E. (2004) De novo emergence of growth factor receptors in activated human $CD4^+$ and $CD8^+$ T lymphocytes. Metabolism 53, 117-122 https://doi.org/10.1016/j.metabol.2003.07.015
  7. Fu, Y. K., Arkins, S., Wang, B. S., and Kelley, K. W. (1991) A novel role of growth hormone and insulin-like growth factor-I. Priming neutrophils for superoxide anion secretion. J. Immunol. 146, 1602-1608
  8. Fukushima, R., Saito, H., Inoue, T., Fukatsu, K., Inaba, T., Han, I. S., Furukawa, S., Jin, M. T., and Muto, T. (1999) Prophylactic treatment with growth hormone and insulin-like growth factor I improve systemic bacterial clearance and survival in a murine model of burn-induced sepsis. Burn 25, 425-430 https://doi.org/10.1016/S0305-4179(98)00188-0
  9. Gibson, L. F., Piktel, D., and Landreth, K. S. (1993) Insulin-like growth factor-I potentiates expression of interleukin-7 dependent pro-B cells. Blood 82, 3005-3011
  10. Hinton, P. S., Peterson, C. A., Dahly, E. M., and Ney, D. M. (1998) IGF-I alters lymphocyte survival and regeneration in thymus and spleen after dexamethasone treatment. Am. J. Physiol. 274, R912-R920
  11. Houle, V. M., Schroeder, E. A., OdIe, J., and Donovan, S. M. (1997) Small intestinal disaccharidase activity and ileal villus height are increased in piglets consuming formula containing recombinant human insulin-like growth factor-I. Pediatr. Res. 42, 78-86 https://doi.org/10.1203/00006450-199707000-00013
  12. Johnson, E. W., Jones, L. A., and Kozak, R. W. (1992) Expression and function of insulin-like growth factor receptor on anti-CD3-activated human T lymphocytes. J. Immunol. 148, 63-71
  13. Kimata, H. and Fujimoto, M. (1994) Growth hormone and insulin-like growth factor I induce immunoglobulin(Ig)E and IgG4 production by human B cells. J. Exp. Med. 180, 727-732 https://doi.org/10.1084/jem.180.2.727
  14. Kooijman, R., and Coppens, A. (2004) Insulin-like growth factor-I stimulates IL-10 production in human T cells. J. Leukocyte Biol. 76, 862-867 https://doi.org/10.1189/jlb.0404248
  15. Kooijman, R., Hooghe-Peters, E. L., and Hooghe, R. (1996) Prolactin, growth hormone, and insulin-like growth factor in the immune system. Adv. Immunol. 63, 377-454 https://doi.org/10.1016/S0065-2776(08)60860-3
  16. Kooijman, R., Willem, M., De Haas, C. J., Rijker, G. T., Schuunnan, A. L., Van Buul-Offers, S. C., Heijnen, C. J., and Zeger, B. J. (1992) Expression of type I insulin-like growth factor receptors on human peripheral blood mononuclear cells. Endocrinol. 131, 2244-2250 https://doi.org/10.1210/en.131.5.2244
  17. Korolkiewicz, R. P., Tashima, K., Fijita, A., Kato, S., and Takeuchi, K. (2000) Exogenous insulin-like growth factor (IGF)-I improves the impaired healing of gastric mucosal lesions in diabetic rats. Pharmacol. Res. 41, 221-229 https://doi.org/10.1006/phrs.1999.0581
  18. Landreth, K. S., Narayanan, R., and Dorshkind, K. (1992) Insulin-like growth factor-I regulates pro-B cell differentiation. Blood 80, 1207-1212
  19. Liu, E., Law, H. K. W., and Lau, Y. L. (2003) Insulin-like growth factor-I promotes maturation and inhibits apoptosis of immature cord blood monocytes-derived dendritic cells through MEK and PI3-kinase pathways. Pediatr. Res. 54, 919-925 https://doi.org/10.1203/01.PDR.0000088067.04673.1B
  20. Mendenhall, C. L., Roselle, G. A., Grossman, C. J., and Gartside, P. (1997) The effects of recombinant human insulin-like growth factor-Ion immunological recovery in the malnourished alcoholic rat. Alcohol Clin. Exp. Res. 21, 1682-1689
  21. Renier, G., Clement, I., Desfait, A. C., and Lambert, A. (1996) Direct stimulatory effect of insulin-like growth factor-Ion monocyte and macrophage tumor necrosis factoralpha production. Endocrinol. 137, 4611-4618 https://doi.org/10.1210/en.137.11.4611
  22. Roldan, A., Charreau, E., Schillaci, R., Eugui, E. M., and Allison, A. C. (1989) Insulin-like growth factor-I increases the mitogenic response of human peripheral blood lymphocytes to phytohemagglutinin. Immunol. Lett. 20, 5-8 https://doi.org/10.1016/0165-2478(89)90060-6
  23. Simmen, F. A., Cera, K. R., and Maha, D. C. (1990) Stimulation by colostrum or mature milk of gastrointestinal tissue development in newborn pigs. J. Animal Sci. 68, 3596-3603
  24. Sorensen, O. E., Cowland, J. B., Theilgaard-Monch, K., Liu, L., Ganz, T., and Borregaard, N. (2003) Wound healing and expression of antimicrobial peptides/polypeptides in human keratinocytes, a consequence of common growth factors. J. Immunol. 170, 5583-5589
  25. Stuart, C. A., Meehan, R. T., Neale, L. S., Cintron, N. M., and Furlanetto, R. W. (1991) Insulin-like growth factor-I binds selectively to human peripheral blood monocytes blood monocytes and B-Iymphocytes. J. Clin. Endocrinol. Metab. 72, 1117-1122 https://doi.org/10.1210/jcem-72-5-1117
  26. Tapson, V. F., Boni-Schnetzler, M., Pilch,. P. F., Center, D. M., Berman, J. S. (1988) Structural and functional characterization of the human T-lymphocyte receptor for insulin-like growth factor I in vitro. J. Clin Invest. 82, 950-957 https://doi.org/10.1172/JCI113703
  27. Tu, W., Cheung, P. T., and Lau, Y. L. (1999) IGF-I increases interferon-gamma and IL-6 mRNA expression and protein production in neonatal mononuclear cells. Pediatr. Res. 46, 748-754 https://doi.org/10.1203/00006450-199912000-00018
  28. Uramkpa, F. O, Ismond, M. A., and Akobundu, E. N. (2002) Colostnun and its benefits. Nutr. Res. 22, 755-767 https://doi.org/10.1016/S0271-5317(02)00373-1
  29. Wright, N. A., Paulson, R., Stamp, G., Elia, G., Ahnen, D., Jeffery, R., Longcroft, J., Pike, C, Rio, M-C., and Chambon, P. (1993) Trefoil peptide expression in gastrointestinal epithelial cells in inflammatory bowel disease. Gastroenterol. 104, 12-20.
  30. Xu, X., Mardell, C., Xian, C. J., Zola, H., and Read, L. C. (1995) Expression of functional insulin-like growth factor-I receptor on lymphoid cell subsets of rats. Immunol. 85, 393-399