• Korean Journal of Agricultural Science
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• v.11 no.2
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• pp.252-263
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• 1984

This study was conducted to find out the effect of thyroid function on Hypophysis, and serum FSH and LH concentrations in female rats. One hundred and forty-four female rats (wistar-imamichi albino rats) of 25 days old were divided into 4 groups; thyroidectomy (Thx.) propylthiouracil (PTU), thyroxine (Thyro.) and control (Cont.) groups. Thirty-six heads of rats were arranged to each group. The thyroid glands of the thyroidectomized groups were removed by surgery. The PTU treated groups were drunk the propylthiouracil solution of 0.05% and the thyroxine treated groups were administered subcutaneously with $30{\mu}g$ per 100 g body weight on 3 days intervals. Every 6 heads of rats in each group were sacrificed at 1, 2, 3, 4, 5, and 6 weeks after treatment with time elapse for investigating the weights and histological changes of hypophysis. The results obtained were as follows : 1. The weights of hypophysis at all treated groups were higher than control group, but significantly increased from 3 to 5 weeks. The significance was not recognized between Thyro. and control groups, and Thx. and PTU groups. 2. In the histological changes of hypophysis, eosinophils atrophied from 4 weeks after treatment in Thx. and PTU groups, and basophils showed hypertrophy and hyperplasia from 2 weeks after treatment and thereafter this tendency was more serious showing vacuolization from 4 weeks after treatment. In Thyro. treated group, eosinophils showed slight hypertrophy and basophils atrophied from 5 weeks after treatment but the group showed the similar histological changes in comparison with the control group. 3. The changes of the concentrations of serum FSH at all observation times were significantly recognized among all observation groups. The concentrations in True and PTU groups were significantly lower than those in control group, but those in Thyro. group were significantly higher than those in control group. 4. The changes of the concentrations of serum LH in all treated groups were significantly lower than those in control group. The significance was not recognized between True and PTU group, and Thyro. and control groups.

• Korean Journal of Agricultural Science
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• v.11 no.1
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• pp.103-107
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• 1984

The present study was carried out to invest igate on changing phases of the concentrations of serum LH, FSH, prolactin, testosterone, GOT and CPT in male pigs at various body weight. Six Duroc ${\times}$ Large White ${\times}$ Landrace boars were used to obtain serial blood samples at approximately 20kg body weight intervals from birth to 130kg body weight. The results obtained in this study were as follows: 1. Serum LH concentrations ranged between 2.89 and 4.25 mIU/ml from birth to 130kg body weight. FSH concentrations were below 1.25 mIU/ml under the limit of detection of the assay. 2. Serum prolactin concentrations were 8.05ng/ml at birth, between 3.07 and 4.25ng/ml from 15 to 50kg body weight, between 7.30 and 6.89 ng/ml from 70 to 90kg body weight and then declined to 4.10 ng/ml at 110kg body weight. 3. Serum testosterone concentrations were 0.42 ng/ml at birth, between 0.11 and 0.09 ng/ml from 15to 30kg body weight, and then increased to 1.15ng/ml at 50kg body weight and remained fairly constant thereafter. 4. Serum GOT concentrations were highest (130.75 karmen units/ml) at birth, and rapidly declined between 58.56 and 65.25 karmen units/ml from 15 to 70kg body weight and then increased 83.60 karmen units/ml at 90kg body weight. 5. Serum GOT concentrations were highest (63.25 karmen ullits/ml) at birth and rapidly declined between 27.25 and 30.75 karmen units/ml from 15 to 70kg body weight and then increased to 41.62 karmen units/ml at 90kg body weight.

• Korean Journal of Agricultural Science
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• v.11 no.2
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• pp.233-243
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• 1984

The present study was carried out to determine the changes of the sex hormone levels in serum throughout the estrous cycle and the gestation period on the Landrace gilts. The blood samples were taken from the vein of six gilts. LH, FSH, prolactin, progesterone, $estradiol-17{\beta}$ and cortisol in serum were analyzed by the radioimmunoassay methods. The results obtained on this study were summarized as follows; 1. The age at puberal estrus was 179.5 days, the weight at puberal estrus was 88.2kg, the length of estrous cycle was 21.3days, the gestation length was 114days and the litter size was 9.5 head in the Landrace gilts. 2. During the estrous cycle, the serum LH and prolactin concentrations were below 1.56mIU/ml and 2.4ng/ml, respectively, under the limit of detection of the assay. The FSH concentrations ranged from 1.50 to 2.20mIU/ml for day 6~15 after the estrus and they were below 1.25mIU/ml from day 3 to day + 3, with day 0 being the first day of the estrus. 3. Progesterone concentrations were 1.90ng/ml at day 0 of the estrus and increased about 13.1ng/ml at day 3 of the estrus, and reached peak levels at day 9. $Estradiol-17{\beta}$ concentrations were below 27.2pg/ml throughout the luteal phase, and reached about 27.2pg/ml at day 0 and day 18. Cortisol concentrations reached peak levels at dey 0 and ranged from 24.65 to 28.57ng/ml throughout the luteal phase. 4. During the gestation period, the concentrations of LH, FSH and prolactin ranged of 3.10~4.37mIU/ml, 1.30~1.80mIU/ml and 2.60~6.70ng/ml, respectively. 5. Progesterone concentrations declined from 38.90~16.85ng/ml throughout the pregnancy to 1.90ng/ml at the time of parturition. $Estradiol-17{\beta}$ concentrations increased from 27.20pg/ml at 15 days after the pregnancy to 620.17pg/ml at the time of parturition. Cortisol concentrations reached peak levels at the time of parturition and ranged from 13.58 to 22.31ng/ml throughout the pregnancy.

• The Journal of Korean Obstetrics and Gynecology
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• v.34 no.3
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• pp.175-191
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• 2021

Objectives: This study aims to analyze the medical record of eight cases of polycystic ovary syndrome (PCOS) in a local Korean medicine clinic, in order to provide evidence on Korean medicine treatment of PCOS and to suggest the direction of future studies. Methods: The medical records of eight cases diagnosed with PCOS based on 2003 Rotterdam Criteria and whose total testosterone level was 0.53 ng/ml and over were retrospectively analyzed. The primary outcome was total testosterone level, and the secondary outcomes included luteinizing hormone (LH) and follicle stimulating hormone (FSH) levels, LH/FSH ratio, weight, last mentrual period (LMP), numerical rating scale (NRS) score of dysmenorrhea, and other associated symptoms pre- and post-treatment. Korean medicine treatment consists of the complex herbal prescription consisting of Yukmijihwang-tang, Baekho-tang, Daesiho-tang, and Gyejibokryeong-hwan, modified according to symptoms; acupuncture and electroacupuncture on 中脘 (CV12), 下脘 (CV10), 關元 (CV4), 石門 (CV5), 合谷 (LI4), 太衝 (LR3), 三陰交 (SP6), and 懸鍾 (GB39); and indirect moxibustion on 神闕 (CV8). Results: The average total testosterone level lowered statistically significantly after the treatment. The average FSH and LH levels lowered with insignificance, while the average LH/FSH ratio and weight lowered statistically significantly. During the treatment period of three to six months, every patient had the menstrual cycle shortened except for one case; and two cases with infertility problem succeeded in being pregnant. There was no adverse event. Conclusions: The Korean medicine treatment was found effective in treating PCOS, especially regarding hyperandrogenism, amenorrhea or oligomenorrhea, and infertility.

• Clinical and Experimental Reproductive Medicine
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• v.33 no.3
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• pp.149-157
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• 2006

Objective: This study was to investigate the clinical efficiency of clomiphene citrate/GnRH antagonist protocol comparing with the clomiphene citrate only protocol in infertile women with normal ovulatory cycles. Method: Among 116 patients, 43 were received assisted reproductive technologies using natural ovulatory cycle, 38 and 35 were received clomiphene citrate only protocol and clomiphene citrate/GnRH antagonist combined protocol, respectively, and the clinical results were compared and analyzed Results: In each group, basal levels of LH, FSH, $E_2$ and FSH, $E_2$ on hCG day injected were not different, but LH level and endometrial thickness on hCG injected day were decreased significantly and the pregnancy rate was increased significantly in clomiphene citrate/GnRH antagonist group. Conclusion: The pregnancy rate was increased significantly in clomiphene citrate/GnRH antagonist group compared with natural ovulatory cycle and clomiphene citrate only group.

• Korean Journal of Agricultural Science
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• v.12 no.1
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• pp.70-74
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• 1985

Serum levels of LH, FSH, prolactin, estradiol-$17{\beta}$ and progesterone were investigated every 20 days after the day of mating for the period of 140 days, at the day of parturition and thereafter 10 th and 20th day postpartum in Korean native goats. Serum levels of LH were highest with 1.95 mIU/ml at the 60 days after gestation, but maintained high levels until 100 days after gestation, and thereafter decreased gradually to 0.02 mIU/ml at the day of parturition. Prolactin concentrations increased from the 140 days after gestation, and showed highest levels with 29.75 ng/ml at the day of parturition, then decreased gradually. FSH levels determined were below 1.25 mIU/ml during the experimental period. Estradiol-$17{\beta}$ concentrations increased gradually from 7.51 pg/ml at the 20 days after gestation to 159.62 pg/ml at the day of parturition, and decreased rapidly after parturition. Progesterone levels were highest with 6.62 ng/ml at the 120 days after gestation, the decreased rapidly to 1.25 ng/ml at the day of parturition.

• Clinical and Experimental Reproductive Medicine
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• v.10 no.2
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• pp.25-37
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• 1983

As the cytogenetic developed, cytogenetic study has also developed progressively. This study is a systematical cytogenetic and clinico-hormonal analysis of 20 cases Wp.ere gonadal dysgenesis was diagnosed and deferred to the Dept. of obstetrics and Gynecology, Yonsei University, Medical School from Jan. 1974 to Aug. 1983. Twenty patients with the diagnosis of gonada dysgenesis have been assesed as to possible correlations between clinical, homonal and cytogenic findings. The desults were as follows; l. Gonadal dysgenesis were found in 20 cases, consisting of 15 cases (75%) of turnurs syndrome, 4 case of pure gonadal dysgenesis (20%), 46. XX and 1 case of mixed gonadal dysgenesis, 45,XO/46,XY. 2. Patients with XO karyotype, turner's ryndorme, have a resonably constant clinical picture of sexual infantilism with streak gonads, short status and webbed neck. 3. 17 cases were found primary amenorhea and two cases were noted with 2 ndary amenorrhea. one case has been presented with menstruation. 4. The rudimentary streak gonads were found in 7 cases of 8 cases and one case has a rudimentary streak gonad on one side and a testis on the contralateral side. 5. The study showed that potients with gonadal dysgenesis had an average of about 4-8 times higher basal FSH and about 3-7 times higher basal LH than that of the early follicular phase of normal menstrual cycle. 6. Two cases of three gonadal dysgenesis patieats, who performed LH-RH challage test, showed that the serum FSH levels reached the maximal level at 30 min after injection of CHRH and the serum LH level reached the maximal level at 60 min ofter injection of LHRH one case showed no significant response to LH-RH injection. Thus, bu studying simultoneously the clinical, cytogenic, hormonal aspects and visualization of gonads, we have gained some practical insight into the requirements for proper disgnosis and treatment.

• Korean Journal of Animal Reproduction
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• v.7 no.1
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• pp.30-40
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• 1983

This experiment was carried out to investigate the effects of unilateral and bilateral ovariectomy in immature rats on the weight of body, ovary, uterus and adrenal gland and the change of serum FSH and LH level. Ninty Sprague-Dawley female rats, 23${\pm}$2 days old, were divided into 3 groups with 30 heads per group; control, unilaterally and bilaterally ovariectomized group. Each group was subdivided into 6 groups according to 6 experimental periods; day 4, 8, 12, 16, 20, and 24 after operation. Five rats at every 4 days intervals were measured their body weights and sacrificed for the measurement of their ovarian, uterine and adrenal weights and at the same time blood samples were taken for the determination of serum FSH and LH level by radioimmunoassay. The following results were obtained: 1. Body weights in the unilaterally and bilaterally ovariectomized goups were higher than those of control groups during all experimental periods, even though there were no significant differences among the above 3 groups. 2. A significant hypertrophy of the remained ovary in the unilaterally ovariecto mized group was observed from day 16 till day 24 after operation. The ovarian weight; 22.1${\pm}$1.73mg, at day 16 in control group was smaller than the unilaterally ovariectomized group weighing 50.5${\pm}$8.45mg (p<0.01) and the ovarian weights, 75.9${\pm}$2.25mg and 63.3${\pm}$7.08mg ; at day 20 and 24 in unilaterally ovariectomized group were significantly larger than 29.1${\pm}$2.33mg and 26.3${\pm}$1.76mg in control group, respectively (p<0.01 and p<0.05). 3. The uterus of bilaterally ovariectomized group were remarkably atrophied from day 8 after operation as compared with those of control and unilaterally ovariectomized group. The uterine weight at day 24 was 96.7${\pm}$9.15mg for control group, 139.4${\pm}$1.73mg for unilaterally ovariectomized group and 21.7${\pm}$1.08mg for bilaterally ovariectomized group, respectively and there were significant differences among 3 groups (p<0.01). 4. A statistically significant increase ofthe weight of adrenal gland was observed at day 16 in the unilaterally ovariectomized group with 24.4${\pm}$2.58mg against 15.5${\pm}$3.09mg in control group and 13.9${\pm}$1.38mg in bilaterally ovariectomized group (p<0.05). The adrenal gland weight in unilaterally ovariectomized group with 24.7${\pm}$1.63mg at day 20 and 31.2${\pm}$1.62mg at day 24 increased significantly as compared with bilaterally ovariectomized group with 15.1${\pm}$13.11mg at day 20 and 15.6${\pm}$1.76mg at day 24. 5. Serum FSH level of unilaterally ovariectomized group increased remarkably up to 2.97${\pm}$0.37mIU/ml at day 4 after operaton and then decreased gradually. Serum FSH level of bilaterally ovariectomized group were higher than those of control group throughout all experimental periods. 6. Serum LH level ofunitelarally ovariectomized group with 3.17${\pm}$0.32mIU/iml at day 4 and 3.57${\pm}$0.58mIU/ml at day 24 increased noticeably more than those of control group with 1.79${\pm}$0.16 mIU/ml at day 4 and 2.17${\pm}$0.27mIU/ml at day 24 (p<0.05).

• 대한생식의학회:학술대회논문집
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• 2002.11a
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• pp.89-90
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• 2002

• Proceedings of the Korean Aquaculture Society Conference
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• 2006.05a
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• pp.369-370
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• 2006