• Korean Journal of Soil Science and Fertilizer
• /
• v.45 no.4
• /
• pp.515-523
• /
• 2012

The study investigated the biochemical methane potential (BMP) assay of cellulose supplementing with mixed methanogens and cellulolytic bacteria to improve anaerobic digestion for methane production. For the BMP assay, 7 different microbial supplementation groups were consisted of the cultures of mixed methanogens (M), Fibrobacter succinogenes (FS), Ruminococcus flavefaciensn (RF), R. albus (RA), RA+FS and M+RA+FS including control. The cultures were added in the batch reactors with the increasing dose levels of 1% (0.5 mL), 3% (1.5 mL) and 5% (2.5 mL). Incubation for the BMP assay was carried out for 40 days at $38^{\circ}C$ and anaerobic digestate obtained from an anaerobic digester with pig slurry as inoculum was used. In results, 5% FS increased total biogas and methane production up to 10.4~22.7% and 17.4~27.5%, respectively, compared to other groups (p<0.05). Total solid (TS) digestion efficiency showed a similar trend to the total biogas and methane productions. Generally the TS digestion efficiency of the FS group was higher than that of other groups showing at the highest value of 64.2% in the 5% FS group. Volatile solid (VS) digestion efficiencies of 68.4 and 71.0% in the 5% FS and the 5% RF were higher than other groups. After incubation, pH values in all treatment groups were over 6.4 indicating that methanogensis was not inhibited during the incubation. In conclusion, the results indicated that the hydrolysis stage for methane production in anaerobic batch reactors was the late-limiting stage compared with the methanogenesis stage, and especially, as the supplementation levels of F. succinogenes supplementation increased, the methane production was increased in the BMP assay compared with other microbial culture addition.

• Korean Journal of Food Science and Technology
• /
• v.53 no.3
• /
• pp.356-363
• /
• 2021

This study investigated the quality characteristics of anchovy sauce fermented using Nuruk to maintain a unique flavor, reduce fishy smell, and improve the fermentation rate. Six kinds of fermented fish sauces, including the control, fermentation using traditional Nuruk; SH Koji (Fs-A), JJ Koji (Fs-B), GJ Koji (Fs-C); and fermentation with improved Nuruk; Aspergillus luchuensis (Fs-D) and Aspergillus oryzae (Fs-E), were prepared. Samples were collected at 15 days intervals with 10% Nuruk added to raw anchovy and fermented at 25o C for 60 days. The free amino acids, especially glutamic acid content and amino nitrogen, were the highest in Fs-C, reflecting the high protease activity of Nuruk C (GJ). Regarding overall sensory evaluation, the control was the lowest, whereas Fs-C was highly evaluated among the sample groups. The addition of Nuruk not only shortened the fermentation period, but also increased the overall sensory level by adding umami and reducing fishy odor.

• Analytical Science and Technology
• /
• v.19 no.3
• /
• pp.263-271
• /
• 2006

The levels of total PCBs, Co-PCBs and PCDD/Fs in the transformer insulation oil samples obtained using GC/ECD and HRGC/HRMS were ranged from N.D. to 77.3 ppm, from 0.0863 to 2.49 ppm and from N.D. to 0.00241 ppm, respectively. In terms of WHO-TEQ values, Co-PCBs and PCDD/Fs were ranged from 23.3 to 600 pgTEQ/g and from N.D. to 128 pgTEQ/g, respectively (${\Sigma}Co$-PCBs+PCDD/Fs concentration was calculated 24.4~728 pgTEQ/g). Although, the contribution of PCDD/Fs was below 12% in total TEQ concentration, it is suggested contamination of PCDD/Fs in transformer insulation oils. Among 10 samples, 4 samples showed higher concentration than 2 ppm (specific waste criterion of Korea) and Aroclor 1242, 1248, 1254 and 1260 was detected in samples as a single or mixture of Aroclor. It was shown reliable relationship between concentration of Co-PCBs and those of PCDD/Fs (p<0.003), however, was not shown between production year of transformer and concentration of PCBs. The distribution pattern of Co-PCB congeners showed that the ratios of mono-ortho substituted congeners were higher than non-ortho substituted congeners. Among that, PCB-118 congener was predominant. In addition, the OCDD congener was predominated in PCDD/Fs congeners as above 53%. Moreover, the congener pattern of Co-PCBs was similar to that of Aroclor as well as ambient air, which suggested that PCBs volatilization from transformer insulation oil affected the pattern of Co-PCBs in ambient air.

• Journal of Life Science
• /
• v.20 no.12
• /
• pp.1872-1881
• /
• 2010

Extracts of soybeans fermented by Bacillus subtilis have a wide variety of functions, such as enhancing the body's immune function, fibrinolysis activity, anti-inflammation, anti-cancer, estrogen function and anti-infection effects. Recently, it was reported that the extracts of fermented beans exhibit strong anti-inflammatory and anti-cancer properties by suppressing the transcription of pro-inflammatory cytokine genes and induction of apoptosis, respectively. However, the mechanisms of their cytotoxicity in human gastric cancer cells are poorly understood. In the present study, we investigated the effects of ethyl alcohol extracts from fermented soybean (FS) and yellow agabean (FYA) on cell growth and apoptosis in AGS human gastric cancer cells. A treatment of FS and FYA inhibited the growth of AGS cells in a concentration-dependent manner by inducing apoptosis. FS- and FYA-induced apoptosis were associated with down-regulation of XIAP and cIAP-2, and up-regulation of pro-apoptotic Bax expression. Moreover, a treatment of FS and FYA not only triggered an increase in the levels of death receptor (DR)4, DR5, Fas and FasL, but also induced the activation of casepase-3, -8 and -9. These findings illustrate that FS and FYA may have a therapeutic potential in human gastric AGS cells and as a functional food.

• Journal of Korean Society for Atmospheric Environment
• /
• v.26 no.5
• /
• pp.517-532
• /
• 2010

After establishment of Banwol industrial complex in 1987, Ansan city becomes the largest industrial sector development in Gyeonggi-do, Korea. As the population and industrial activity grow over this region, toxic air pollutants, particularly POPs (Persistent Organic Pollutants) from various emission sources have been major public concerns. Air samples for POPs monitoring were collected at the industrial sites ($A_2$), residential sites ($B_1$, $B_2$), commercial site (C), and rural/remote site (D) of the area of Ansan during 2008 with a prolonged industrial sampling site $A_1$ from 2001 to 2008. All samples were analysed for 2,3,7,8 substituted-polychlorinated dibenzo-p-dioxin and dibenzofurans (PCDD/Fs) and dioxin like polychlorinatd diphenyls (dl-PCBs). In site $A_1$, a steady decline of their concentrations from 2003 to 2008 was observed due to the reinforced emission guideline from waste incinerators. The average concentration of the PCDD/Fs and dl-PCBs ranged between 0.118 pg-TEQ/$m^3$ (rural/remote site D) and 0.532 pg-TEQ/$m^3$ (industrial area $A_2$). These level were generally consistent with previous studies in Gyeonggi-do, while higher than other places. Most of PCDD/Fs congener were partitioned into particle phase, whereas dl-PCBs were partitioned into gas phase. The logarithm of gas-particle partition coefficient $K_P$ of dl-PCBs and PCDD/Fs were well correlated with sub-cooled liquid vapor pressure $P_L$. The slope $m_T$ of log $K_P$ versus log $P_L$ for PCDD/Fs (-1.22) and dl-PCBs (-1.02) in industrial area ($A_2$) were high compared to other residential/commercial area. It suggests that this area was likely influenced by the direct emission source of PCDD/Fs and dl-PCBs. To simulate the partition of PCDD/Fs and dl-PCBs between gas and particle phase, Junge-Pankow model ($P_L$-base) and $K_{oa}$ model were applied. It was found that J-P model was more suitable than the $K_{oa}$ model in this study.

• Analytical Science and Technology
• /
• v.18 no.1
• /
• pp.13-22
• /
• 2005

The elution patterns of seventeen 2,3,7,8-substituted PCDDs/Fs and 12 dioxin-like PCBs were studied by both manual open columns and automatic parallel LC columns in cleanup procedure. PCDDs/Fs and non-ortho-PCBs from other mono-ortho-PCBs were separated on automatic LC column, whereas they were not separated on an open manual column. The elution study on two cleanup methods was carried out using the PAR solution of unlabeled congeners and checked the recovery of each congener. Total recoveries of cleanup fractionation were ranged between 61.9 ~ 96.0% for PCDDs/Fs and 70.4 ~ 79.0% for PCBs by manual open columns and 71.8 ~ 104.5% for PCDDs/Fs and 61.3-120.3% for PCBs by automatic parallel LC columns, respectively. Unfortunately, #169-HxCB and 1,2,3,7,8-PeCDD were not separated on DB-5MS capillary column. The ions of 1,2,3,7,8-PeCDD were selected at M/M+2 instead of M+2/M+4 suggested by EPA method 1613. It is possible to discriminate 1,2,3,7,8-PeCDD and PCB #169 in HRGC/HRMS analysis.

• Asian-Australasian Journal of Animal Sciences
• /
• v.29 no.2
• /
• pp.195-203
• /
• 2016

This study was conducted to evaluate the effect of dietary supplementation with rice bran, flax seed, or sunflower seed to finishing native Korean cattle (Hanwoo) on growth performances, carcass characteristics, fatty acid composition, free amino acid and peptide contents, and sensory evaluations of Longissimus muscle (LM). A total of 39 Hanwoo steers (average age of 22.2 mo and average body weight (BW) of 552.2 kg) were randomly divided into Control, rice bran (RB), flax seed (FS), or Sunflower seed (SS) groups. The steers were group fed for 273 d until they reached an average age of 31.2 mo. Final BW was 768.2, 785.8, 786.2, and 789.0 kg, and average daily gain was 0.79, 0.85, 0.82, and 0.84 kg for the Control, RS, FS, and SS groups, respectively (p>0.05). Fat thickness of the FS group (19.8 mm) was greater (p<0.05) than that of the other groups. Final yield grade converted into numerical values was 2.0 for the RB group, 1.7 for the Control and SS groups, and 1.4 for the FS group. Marbling degrees for the Control, SS, RB, and FS groups were 5.3, 5.1, 4.7, and 4.6, respectively. Percentages of palmitic acid ($C_{16:0}$), stearic acid ($C_{18:0}$), and arachidic acid ($C_{20:0}$) in the LM were not different among the groups. Palmitoleic ($C_{16:1}$) acid was higher (p<0.05) in the SS group. The concentration of oleic acid was highest (p<0.05) in the Control group (47.73%). The level of linolenic acid ($C_{18:3}$) was 2.3 times higher (p<0.05) in the FS group compared to the other groups. Methionine concentration was (p<0.05) higher in FS (1.7 mg/100 g) and SS (1.2 mg/100 g) steers than in the Control or RB groups. Glutamic acid and ${\alpha}$-aminoadipic acid (${\alpha}$-AAA) contents were (p<0.05) higher in the FS group compared to the other groups. LM from the FS group had numerically higher (p>0.05) scores for flavor, umami, and overall palatability in sensory evaluations. In conclusion, supplementation of flax seed to diets of finishing Hanwoo steers improved sensory evaluations which might have been caused by increases in flavor related amino acids such as methionine, glutamic acid and ${\alpha}$-AAA and peptides, anserine and carnosine, and their complex reactions.

• Journal of Korean Society of Environmental Engineers
• /
• v.33 no.12
• /
• pp.900-906
• /
• 2011

To investigate concentration and sources of PCDD/Fs and Co-PCBs in sediments, total 52 sediment samples were measured at 17 sites in Geum River. As a result of analysis, total concentrations of PCDD/Fs and Co-PCBs were ranged from not detected (N.D.)~193.47 pg/g-d.w. (mean value: 84.52 pg/g-d.w) and 0.34~359.19 pg/g-d.w (mean value: 114.65 pg/g-d.w.) respectively. Also, toxic equivalent (TEQ) concentration of PCDD/Fs and Co-PCBs were ranged from N.D.~5.12 (mean value: 0.88) pg I-TEQ/g-d.w and N.D.~0.58 (mean value: 0.09) $WHO_{2005}$-TEQ pg/g-d.w. respectively. The upstream indicate the highest concentration and as it goes to the downstream, it has tendency to decrease. Higher chlorinated compounds were dominant in most of site which detected PCDD/Fs. Occurrence of Co-PCBs isomer patterns appear similar to each sampling site was found. The isomer patterns showed similar between sediment and PCB products. The principal component analysis also showed that the samples contained the characteristics of PCBs products.

• Korean Journal of Agricultural and Forest Meteorology
• /
• v.21 no.4
• /
• pp.269-276
• /
• 2019

A semi-greenhouse experiment with field grown soybean (Glycine max L. cv Deawon, Uram, Jinpong, Soyon, Pungsangnamul, and Haewon) was performed in 2018. The experiment was aimed to investigate individual impacts of drought stress on soybean characteristic and seed yield. The three treatments were used in non-water stress (Control) during the soybean growth season, vegetative stage stress (VS), and flowering period stress (FS). Leaf number, LAI, leaf nitrogen concentration, and leaf biomass were decreased by drought at R4. In our study, the number of pods was 33.6% and 40.5% lower, respectively, in control than in VS and FS. In 100 seed weight, was 16.1% and 10.1% lower, respectively, in control than in VS and FS at R8. As a result, seed yield was 39.8% and 45.1% lower, respectively, in control than in VS and FS. Depending on the drought period, Daewon and Haewon showed a large decrease in yield, while Soyon did not change. The results of this study showed that flower and beginning pod setting stage responded more sensitively to the drought period than vegetative stage. Overall, these results demonstrate soybean seed yield formation more sensitive the during the flowing and beginning pod setting stage. We conclude that adequate water supply for pod setting stage, guaranteeing a high seed yield.

• Journal of the Korean earth science society
• /
• v.30 no.2
• /
• pp.234-246
• /
• 2009

Characteristics of speckle errors of Sea-viewing Wide Field-of-view Sensor (SeaWiFS) chlorophyll-${\alpha}$ concentration were analyzed, and its causes were investigated by using SeaWiFS data in the East Sea from September 1997 to December 2007. The speckles with anomalously high concentrations were randomly distributed and showed remarkably high bias of greater than $10mg/m^3$, compared with their neighboring pixels. The speckles tended to appear frequently in winter, which might be related to cloud distribution. Ten-year averaged cloudiness of winter was much higher over the southeastern part, with frequent speckles, than the northwestern part of the East Sea. Statistical analysis results showed that the number of the speckles was increased as cloudiness increased. Normalized water-leaving radiance of the speckle pixel was considerably low at the short wavelengths (443, 490, and 510 nm), whereas the radiance at 555 nm band was normal. These low measurements produced extraordinarily high concentration from the chlorophyll-${\alpha}$ estimation formula. This study presented the speckle errors of SeaWiFS chlorophyll-${\alpha}$ concentration in the East Sea and suggested that more reliable chlorophyll-${\alpha}$ data based on appropriate ocean color remote sensing techniques should be used for the oceanic application researches.