• Title/Summary/Keyword: FRT

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Construction of Recombinant Bombyx mori Nuclear Polyhedrosis Virus Using a FLP/FRT System of Yeast, Saccharomyces cerevisiae 2$\mu$m plasmid (Yeast의 FLP/FRT 시스템을 이용한 BmNPV의 유전자 재조합)

  • 강석우;윤은영
    • Journal of Sericultural and Entomological Science
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    • v.40 no.1
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    • pp.52-59
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    • 1998
  • For the construction of plasmid and bmNPV sarrying the FRT recognition site for the FLP recombinases, we synthesized the wild type FRT dligonucleotides. The target FRT sequences consist of three 13bp repeated DNA sequences; two repeats in a direct orientation and one inverted relative to the other two. In addition, there is an 8bp spacer region between the repeats which determune the orientation of the FRT recombination site. In order to place the FRT site both in target BmNPV genome and the transfer vector, we constructed a plasmid, FRT site both in the target BmNPv genome and the transfer vector, we constructed a plasmid, pFRT$\beta$-gal, carrying the FRT sites within the cloning sites of pSV vector and a recombinant BmNPV, vFRTPH, carrying the FRT sites at a downstream of polyhedrin promotor, respectively. In order to test the functionality of the FLP/FRT site-specific recombination system, vFRTPH, pFRT$\beta$-gal and pHsFLP DNA were co-transfected into BmN-4 cells. The resulting recombinant virus was designated a vFRT$\beta$2-gal. From construction analysis of the vFRT$\beta$2-gal with PCR technique it was concluded that the entire pFRT$\beta$-gal plasmid with $\beta$-galactosidase gene and origines of replication flanked by two functional hybrid FRT sequences. The efficiency of recombination was 8.7%, which was higher than that(2.2%) of recombination between a conventional transfer vector and the wild type BmNPV.

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Correlation of Berg Balance Scale and Functional Reach Test

  • Park, Eun-Young;Kim, Won-Ho
    • Physical Therapy Korea
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    • v.14 no.4
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    • pp.28-34
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    • 2007
  • The purposes of this study were to provide the basic data and investigate the reliability of functional reach test and identify correlation of Berg balance scale (BBS) and functional reach test (FRT). The subjects were twenty healthy young adults and forty-five over 65 years old in order to compare balance ability. These data were analyzed by independent t-test and Pearson's correlation test using SPSS WIN 10.0. The results were as follows. Intrarater reliability coefficients of FRT was .976 and interrater was .942. FRT was significantly correlated with age, height, and BBS (p<.05). There were no significant differences in FRT and BBS by sex. There was significant difference in reach distance between below 74 elderly and above in FRT. FRT is very reliable test for balance and significantly correlated with BBS. Therefore, it is suggested that FRT is a clinically useful tool to substitute for BBS measuring balance ability in the elderly.

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Effects of Feeding Ferritin Gene Transferred Yeast (Saccharomyces serevisiae) on Performance, Iron Concentration in Organs and Egg of Chickens (Ferritin 유전자 전이 효모(Saccharomyces serevisiae)의 급여가 닭의 생산성, 장기 및 계란의 철분함량에 미치는 영향)

  • Ryu, Byeong-Seon;Park, Jae-Hong;Kim, Dae-Hyeok;Ryu, Kyeong-Seon
    • Korean Journal of Poultry Science
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    • v.30 no.4
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    • pp.245-251
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    • 2003
  • Three experiments were conducted to investigate the effect of feeding yeast accumulated transgenic ferritin(FRT, Saccharomyces cerevisiae) as a probiotic on the performance, iron contents in the liver, spleen, bone and yolk of laying hens and broiler chicks. Effects of feeding FRT were compared with that of feeding wild-type yeast(W0) and yeast grown on 20 mM ferric citrate-added medium (W20). In Expt 1, to investigate the effect of feeding yeast (control, W0 FRT) on performance and iron content of organs of broiler chicks which were fed basal diet supplemented with 75mg/kg iron(Fe75) or not (Fe0), three hundred sixty one-day-old male broiler chicks were fed a corn-sov based diet for five weeks. Weight gain, feed intake and feed conversion were measured weekly. In Expt 2, fifteen 33-week-old ISA Brown laying hens were placed in individual cages and were fed control, W0 and FRT diets for Four weeks. In Expt 3, twenty four 45-week-old ISA Brown laying hens were placed in individual cages and were fed a basal diet for a week. Then, experimental diets (control, W0, W20, FRT) were fed for three weeks. Iron contents in the liver, heart, spleen and tibia were determined at the end of all experiments. Iron content in yolk was measured weekly (expt 2, 3). The level of yeast added and iron concentration of FRT were $1{\times}10^8$cfu/kg diet and 500 mg/kg cell (DM) respectively in Expt 3, yeast was supplemented at $2{\times}10^{10}$cfu/kg diet and the iron content of FRT was 1000mg/kg cell (DM). In Expt 1. birds fed Fe75 showed significantly higher weight gain compared with Fe0 (P<0.05). However, weight gain and feed intake of birds fed FRT was significantly lower than control (P<0.05). In Expt 2, the iron content of the liver was decreased in the FRT treatment (P<0.05). In Expt 3, iron concentration of the liver and spleen tended to be increased by feeding FRt. However, the iron content of the tibia tended to be decreased in the FRT treatment. These results suggest that feeding FRT as a probiotic cannot improve performance and iron content in organs of broiler chicks and laying hens.

Flavonoids of Rosa roxburghii Tratt Act as Radioprotectors

  • Xu, Ping;Zhang, Wen-Bo;Cai, Xin-Hua;Lu, Dan-Dan;He, Xiao-Yang;Qiu, Pei-Yong;Wu, Jiao
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.19
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    • pp.8171-8175
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    • 2014
  • Background: To study the radioprotective effects of flavonoids from Rosa roxburghii Tratt (FRT). Materials and Methods: The radioprotective effects of FRT were investigated by examining cell viability, 30-day survival of mice and the number of colony-forming units in spleen (CFU-S) after total-body 60Co irradiation. Results: The survival rates of irradiated cells gradually increased with increasing concentrations of FRT. The survival rate was the highest at 87% with a concentration of $30{\mu}g/mL$. Pretreatment with FRT was needed to realize its radioprotective activity in mice at the dose of 60 mg/kg. With the increasing doses of 30 mg/kg, 60 mg/kg and 120 mg/kg, the numbers of CFU-S increased, and were significantly different compared with the control group. Conclusions: Pretreatment with FRT prior to irradiation resulted in significantly higher cell survival at 24 h after 5 Gy radiation, increased 30-day survival in mice after exposure to a potentially lethal dose of 8 Gy, and resulted in a higher number of CFU-S in mice after exposure to a dose of 6 Gy. These results collectively indicate that FRT is an effective radioprotective agent.

Planar integrated optics for implementation of fractional fourier transform (분수차 퓨리에 변환을 위한 평판집적 광학계 구현)

  • 박선택;김필수;오차환;송석호
    • Korean Journal of Optics and Photonics
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    • v.7 no.4
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    • pp.333-340
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    • 1996
  • We have implemented a planar integrated optics for the fractional Fourier transform (FRT) which has recently been developed as a generalized form of the conventional Fourier transform. FRT optical systems provide versatile tools for analyzing signals and designing hardwares, but require high accuracy and stability in the arrangement of optical components because of their shift-variant characteristic. The planar optical FRT setup composed of free-space optical components integrated on a single glass block makes the FRT of 2-dimensional(2-D) input patterns through the 3-D glass-space. Therefore, taking advantage of the compactness, easy alignment and thermal/mechanical stability, the planar optics can provide a useful approach to realizing an optical fractional correlation system in a practical way. In the experiment, we have obtained accurate FRT results by using the planar integrated optics with 4 different fractional orders of 0.25, 0.5, 0.75, and 1.0.

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Analysis of Spasticity and Balance of Lower Extremity on Swiss Ball Lumbar Stabilization Exercise(LSE) in Patients with Stroke (스위스볼 요부안정화운동에 따른 뇌졸중 환자 하지의 경직도와 균형 분석)

  • Ko, Dae-Sik;Kim, Chan-Kyu;Jung, Dae-In
    • The Journal of the Korea Contents Association
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    • v.11 no.3
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    • pp.262-270
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    • 2011
  • This study examines the effects of spasticity, equilibrium and gait ability at lower extremity on lumbar stabilization exercise(LSE) with Swiss ball. This experiment was conducted to compare BBS, FRT, MAS and TUG by Swiss ball LSE with 34 stroke patients. equilibrium by BBS or FRT, spasticity by MAS and gait ability by TUG were measured after a 8-week traditional exercise physical therapy(17 subject of stroke patient) and a Swiss ball LSE(17 subject of stroke patient). These result lead us to the conclusion that BBS & FRT were statistically increased and MAS and TUG were decreased on Swiss ball LSE group. there were statistically differential effect between each group on FRT and MAS. These results suggest that Swiss ball LSE has the capability to improve spasticity, equilibrium and gait ability at lower extremity. Consequently, Swiss ball LSE would be lead to restoration of muscle spasticity, equilibrium and gait ability on stroke patients.

Correlations between SPPB, FRT, and TUG in Hospitalized Frail People - The Timed Up and Go test, the Functional Reaching Test, and the Short physical performance battery - (노인요양병원 입원환자의 TUG, FRT와 SPPB와의 상관관계 연구)

  • Park, Rae-Joon;Son, Ho-Hee;Cho, Jeung-Sun;Oh, Hyen-Ju;Lee, Hyeon-Hee;Lee, Moon-Hwan;Kim, Seong-Gil
    • The Journal of Korean Physical Therapy
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    • v.23 no.2
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    • pp.17-21
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    • 2011
  • Purpose: The purpose of this study was to determine the correlations, if any, between SPPB (Short physical performance battery), FRT (Functional reach test), and TUG (Timed up and go test) in hospitalized frail people. Methods: Seventy frail elderly patients (male/female: 16/54) participated in this study. All subjects could walk with or without an assisting device and they had no mental problems. Subjects were scored on physical functioning using the SPPB, FRT, and TUG. The data were analyzed using Pearson Correlation Coefficients. Results: There was a statistically significant difference between (a) SPPB and (b) FRT (p<0.01) and TUG (p<0.01). SPPB and FRT scores showed a positive correlation (r = 0.38) and SPPB and TUG scores showed a negative correlation (r = -0.56). Conclusion: SPPB, FRT, TUG scores are correlated with each other. From these results, we conclude that the SPPB is a useful method for predicting walking, balance, and physical function in frail elderly people.

Quality Characteristics of Rice Paper Roll with Frozen Rainbow Trout Oncorhynchus mykiss Meat Treated with Polysaccharide and TGase (다당류 및 TGase를 처리한 동결 무지개송어(Oncorhynchus mykiss)를 활용한 라이스페이퍼롤의 품질 특성)

  • Hye Min Park;Jong Bong Lee;Byoung Kyu An;Woo Jin Lee;Jung-Jin In;Hyeong Gu Han;Seung Ah Son;Yeon Joo Bae;Kil Bo Shim
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.56 no.5
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    • pp.585-595
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    • 2023
  • This study was conducted to optimize the rice paper roll processing conditions with frozen rainbow trout Oncorhynchus mykiss meat (RPR-FRT) treated with polysaccharide and TGase using a response surface methodology and to examine their quality characteristics. The RSM results for the RPR-FRT showed that the optimum condition for the garlic-pepper mixture was 21.2 g and that for starch was 22.6 g based on 150 g of RPR-FRT. The RPR-FRT contained 58.47 g/100 g of moisture, 8.57 g/100 g of crude protein, 3.28 g/100 g of crude lipid, and 1.00 g/100 g of ash. The vitamin E content was 1,010.91 ㎍/100 g. Based on their contents, the samples could be considered good supplements for P, Cr, and Se. The RPR-FRT contained unsaturated fatty acids (75.84%), DHA (10.33%), and EPA (2.56%). Anserine, arginine, glycine, and taurine accounted for 41.93%, 11.27%, 7.13%, and 7.00% of free amino acids in the RPR-FRT, respectively. The sulfur compounds in the RPR-FRT constituted 73.16% of the total flavor compounds. The RPR-FRT prepared using the optimum conditions was superior in masking off-flavor and showed improved nutritional content.

Establishment of Visible & Invisible Factors for FRT Design (FRT 디자인을 위한 가시적, 비가시적 요인 구축)

  • Han Suk-Woo
    • Proceedings of the KSR Conference
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    • 2004.10a
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    • pp.1701-1706
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    • 2004
  • FRT design aims at high-tech vehicles of high efficiency and cleanness, it must materialize the meaning and role as new added value by applying car design aesthetics of a new future-oriented concept. In addition, different from ordinary vehicle design, FRT involves invisible factors such as pleasantness, safety, accessibility, controllability, movability, conveniency, culture, identity, brand image and fashionableness. Because FRT vehicles are transportation means used by various groups of people, they must be approached with the barrier-free design concept. Therefore, in order to enhance the potential of future transportation culture emphasizing creativity based on diversity, it is important to establish core design factors that create new added values and to apply new concepts to their roles.

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A study of urban FRT's Exterior Design (도시형 FRT Exterior Design 연구)

  • Kim, Seong-Nam;Kim, Sang-Joong;Kim, Chang-Hyun
    • Proceedings of the KSR Conference
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    • 2004.10a
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    • pp.1757-1764
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    • 2004
  • It is the urgent task of induce the inhabitants to utilize the public transportation instead of the personal transportation to dissolve the traffic jam. But construction of the subway, the representative substitute means, demands a great of investment expense including various kinds of expenses. So the technical development and research of standardization of FRT(Fuel cell Rubber tired Train), the eco-friendly public transportation system low in construction expenses, effective, excellent in approach as well as in traffic, are propelled positively. This project wants to propose exterior design FRT in which original creative methods are utilized with the target of development and construction of FRT which coincide with anthropocentric eco-friendly traffic system. Utilizing the proposed style as the basic materials, this project wants to grasp difficulties, and to establish the way to solve and develop them.

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