Effects of Feeding Ferritin Gene Transferred Yeast (Saccharomyces serevisiae) on Performance, Iron Concentration in Organs and Egg of Chickens

Ferritin 유전자 전이 효모(Saccharomyces serevisiae)의 급여가 닭의 생산성, 장기 및 계란의 철분함량에 미치는 영향

  • Ryu, Byeong-Seon (Department of Animal Resource and Biotechnology, Chonbuk National University) ;
  • Park, Jae-Hong (Department of Animal Resource and Biotechnology, Chonbuk National University) ;
  • Kim, Dae-Hyeok (Institute of Molecular Biology and Genetics, Chonbuk National University) ;
  • Ryu, Kyeong-Seon (Institute of Agricultural Science and Technology, Chonbuk National University)
  • 유병선 (전북대학교 동물자원과학과) ;
  • 박재홍 (전북대학교 동물자원과학과) ;
  • 김대혁 (전북대학교 유전공학연구소) ;
  • 류경선 (전북대학교 농업과학기술연구소)
  • Published : 2003.01.01

Abstract

Three experiments were conducted to investigate the effect of feeding yeast accumulated transgenic ferritin(FRT, Saccharomyces cerevisiae) as a probiotic on the performance, iron contents in the liver, spleen, bone and yolk of laying hens and broiler chicks. Effects of feeding FRT were compared with that of feeding wild-type yeast(W0) and yeast grown on 20 mM ferric citrate-added medium (W20). In Expt 1, to investigate the effect of feeding yeast (control, W0 FRT) on performance and iron content of organs of broiler chicks which were fed basal diet supplemented with 75mg/kg iron(Fe75) or not (Fe0), three hundred sixty one-day-old male broiler chicks were fed a corn-sov based diet for five weeks. Weight gain, feed intake and feed conversion were measured weekly. In Expt 2, fifteen 33-week-old ISA Brown laying hens were placed in individual cages and were fed control, W0 and FRT diets for Four weeks. In Expt 3, twenty four 45-week-old ISA Brown laying hens were placed in individual cages and were fed a basal diet for a week. Then, experimental diets (control, W0, W20, FRT) were fed for three weeks. Iron contents in the liver, heart, spleen and tibia were determined at the end of all experiments. Iron content in yolk was measured weekly (expt 2, 3). The level of yeast added and iron concentration of FRT were $1{\times}10^8$cfu/kg diet and 500 mg/kg cell (DM) respectively in Expt 3, yeast was supplemented at $2{\times}10^{10}$cfu/kg diet and the iron content of FRT was 1000mg/kg cell (DM). In Expt 1. birds fed Fe75 showed significantly higher weight gain compared with Fe0 (P<0.05). However, weight gain and feed intake of birds fed FRT was significantly lower than control (P<0.05). In Expt 2, the iron content of the liver was decreased in the FRT treatment (P<0.05). In Expt 3, iron concentration of the liver and spleen tended to be increased by feeding FRt. However, the iron content of the tibia tended to be decreased in the FRT treatment. These results suggest that feeding FRT as a probiotic cannot improve performance and iron content in organs of broiler chicks and laying hens.

Ferritin 유전자 이식 효모 (FRT, Saccharomyces cerevisiae)를 생균제로 급여시에 육계와 산란계의 생산성과 장기 및 난황의 철분 함량에 미치는 영향을 구명하기 위하여 3회의 사양실험을 실시하였다. FRT의 급여효과는 일반 효모 (W0)와 20mM의 구연산 철을 첨가한 배지에서 배양한 효모(W20)의 급여시와 비교하였다. 실험 1에서는 철분을 첨가급여구(75mg/kg; Fe75), 무첨가구 (Fe0) 효모의 급여(무첨가, W0, FRT)가 육계의 생산성과 장기의 철분함량에 미치는 영향을 구명하기 위하여 1일령 육계 수컷 420수를 이용하여 5주간 사양실험을 실시하였다. 매주 증체량과 사료섭취량, 사료요구율을 측정하였다. 실험 2에서는 33주령 이사브라운 산란계 15수를 산란케이지에 개체수용하여 대조구와 W0, FRT 사료를 3주간 급여하였다. 실험 3은 45주령 이사브라운 산란계 24수를 산란케이지에 개체수용하여 1주간 기초사료를 급여한 뒤 시험사료(대조구, W0, W20, FRT)를 3주간 급여하였다. 모든 실험의 종료시 간과 심장, 비장, 경골의 철분함량을 측정하였으며 주간별로 난황의 철분함량을 측정하였으며 주간별로 난황의 철분함량을 측정하였다.(Expt 2, 3) 실험 1과 2에서 효모의 급여량은 사료에 $1{\times}10^8$cfu/kg이었으며 철분함량은 세포 건물기준 500mg/kg이었다. 실험 3에서는 사료에 $2{\times}10^{10}$cfu/kg을 첨가하였으며 철분함량은 1000mg/kg이었다. 실험 1에서 Fe75의 급여는 Fe0에 비해 증체량이 현저히 증가하였다.(P<0.05). 실험 3에서 FRT의 급여는 간과 비장의 철분함량을 증가시키는 경향을 보였으나 경골의 철분함량은 감소하는 경향을 보였다. 이상의 결과에서 FRT의 급여는 생균제로서 육계와 산란계의 생산성과 장기의 철분함량을 개선하지 못하였다.

Keywords

References

  1. AOAC 1994 Official Methods of Analysis (16th Ed.). Associationof official Analytical Chemists. Washinton DC.
  2. Aoyagi S, Baker DH 1995 Iron requirement of chicks fed asemipurified diet based on casein and soy proteinconcentrate. Poult Sci 74:412-415.
  3. Bhattacharya RK, Esh GC 1964 Metabolic relationship betweendietary protein and iron. 1lI. Influence of protein and itsbreakdown products on iron absorption in partially irondepleted rats. Indian J Biochem I:142.
  4. Davis PN, Norris LC, Kratzer FH 1968 Iron bioavai1ability incalcium and phosphorus sources. J Anim Sci 67(Suppl.1):253. (Abstr.)
  5. Deeley RG, Mullinix DP, Wetekam W, Kronenberg 8M,Meyers M, Eldridge JD, Goldberger RF 1975 Vitellogeninsynthesis in the avian liver. Vitellogenin is the precursorof the egg yolk phosphoproteins. J Biol Chern 250:9060-9066.
  6. Erdman JW Jr 1979 Oilseed phytates: nutritional implications.J Am Oil Chern Soc 56:736-741.
  7. Forbes GB, Reina JC 1972 Effect of age on gastrointestinalabsorption (Fe, Sr, Pb) in the rat. J Nutr 102:647-652.
  8. Harrison PM, Arosio P 1996 The ferritins: molecular properties,iron storage function and cellular regulation. BiochimBiophys Acta 1275:161-203.
  9. Henry PR, Miller ER 1995 Iron bioavailability. Pages 169-200in: Bioavailability of Nutrients for Animals: Amino Acids,Minerals, and Vitamins. Ammerman CB, Baker DH andLewis AJ, ed Academic Press San Diego CA
  10. Kegley EB, Spears JW, Flowers WL, Schoenherr WD 2002Iron methionine as a source of iron for the neonatal pig.Nutrition Research 22:1209-1217.
  11. Line JS, Bailey JS, Cox NA, Stem NJ, Tompkins T 1998 Effectof yeast supplemented feed on salmonella and campylobacter population in broilers. Poult Sci 77:405-410.
  12. Morris ER 1987 Iron. Pages 79-142 in: Trace Elements inHuman and Animal Nutrition. 5th ed Vol I Mertz W edAcademic Press Tnc New York NY.
  13. National Research Council 1994 Nutrient requirements ofpoultry (9th Ed). National academy press Washington DCUSA.
  14. Onifade AA, Odunsi AA, Babatunde GM, Olorede BR, MumaE 1999 Comparison of the supplemental effects ofSaccharomyces cerevisiae and antibiotics in low-proteinand high-fibre diets fed to broiler chickens. Arch Tierernahr52:29-39.
  15. Richards MP 1989 Influence of egg production on zinc, copperand iron metabolism in the turkey hen tMeleagrisgallopavo). Comparative Biochemistry and Physiology PartA: Physiology 93:811-817.
  16. SAS/STAT 1996 SAS user guide. release 6.12 edition, SAS InstInc Cary NC.
  17. Scott ML, Nesheim MC, Young RJ 1982 Essential inorganicelements. Pages 277-382 in : Nutrition of the chicken. 3rded WF Humphrey Press Inc Geneva New York.
  18. Shin YM, Kwon TH, Kim KS, Chae KS, Kim DH, Kim JH,Yang MS 2001 Enhanced iron uptake of Saccharomycescerevisiae by heterologous expression of a tadpole ferritingene. Applied and Environmental Microbiology 67:12801283.
  19. Stanley VG, Ojo R, Woldesenbet S, Hutchinson DH, KubenaLF 1993 The use ot:Saccharomyces cerevisiae to suppressthe effects of aflatoxicosis in broiler chicks. Poult Sci72:1867-72.
  20. 박재홍 류명선 김상호 나종삼 김종승 류경선 2003 효모배양물의 첨가사료가 계사내 유해가스 발생 및 육계의 생산성에 미치는 영향. 한국동물자원과학회지 45(1):41-48.
  21. 이현우 김인호 김춘수 손중천 1997 효모의 급여가 육계의 성장 및 장내 대장균의 변화에 미치는 영향. 한국가금학회지 24(2):67-72.