• Toxicological Research
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• 제20권1호
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• pp.63-69
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• 2004

Casapse-3 protease is known as a key role of apoptotic enzyme, and caspase-3 activity is a central event that occurs upstream of DNA fragmentation during apoptosis. This study demonstrates that chitosan pretreatment inhibits cadmium-induced apoptosis by attenuating the activity of caspase-3. We also analyzed the protective effect of chitosan on DNA fragmentation induced by cadmium. Cadmium toxicity was examined by DNA fragmentation and nuclear condensation with Hoechst stain. Caspase-3 activities were increased cadmium treated group for 3 hours compared with control. When chitosan (150 mg/ml) was pretreated at 30 min before cadmium treatment, cadmium cytotoxicity was suppressed in a dose-dependent manner evaluated by DNA fragmentation and caspase activity. From these results, it is suggest that the protective effect of chitosan pretreatment against cadmium-induced cytotoxicity is mediated through inhibition of caspase-3 protease activation and DNA fragmentation.

• 한국산림과학회지
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• 제108권3호
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• pp.454-467
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• 2019

이 연구는 산지를 이용한 산지경관 파편화 모니터링을 제안하고 실효성을 검토하여 다음의 결과를 도출하였다. 첫째, 산지경관이 최근 9년간 빠르게 파편화되고 있어서 국가적 모니터링과 관리가 시급하다. 둘째, 산지경관(GIS) 기반의 평면적 파편화 조사만으로는 중요한 생태적 변화를 확인할 수 없으므로 다양한 조사체계와의 연계가 필요하다. 셋째, 산지경관 파편화 모니터링을 환경영향평가 재해영향평가 등과 연계하여 사전예방원칙을 제도화해야 한다.

• Mass Spectrometry Letters
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• 제7권4호
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• pp.96-101
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• 2016

To determine the influence of the cationization agent on the collision activated dissociation (CAD) fragmentation behavior of oligosaccharides, the CAD spectra of the singly protonated, sodiated oligosaccharides and singly sodiated and dibenzo-18-crown-6 ether conjugated oligosaccharides were carefully compared. Each of these three different species showed quite different fragmentation spectra. The comparison of singly protonated and sodiated oligosaccharide CAD spectra revealed that different cationization agents affected the cationization agent adduction sites as well as the fragmentation sites within the oligosaccharides. When the mobility of $Na^+$ was limited by the dibenzo-18-crown-6 ether encapsulation agent, the examined linear oligosaccharides showed fragmentation patterns quite different from the unmodified ones. For the dibenzo-18-crown-6 ether conjugated oligosaccharides, the charge-remote fragmentation pathways were more likely to be activated than the chargedirected pathways. This work demonstrates that dibenzo-18-crown-6 ether conjugation can potentially provide a route to selectively activate the charge-remote fragmentation pathways, albeit to a limited extent, in tandem mass spectrometry studies.

• 한국조경학회지
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• 제35권6호
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• pp.37-47
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• 2008

Recently, reckless exploitation of land resources without much consideration for the environmental value of the land has been witnessed to accommodate the ever-increasing demands for regional development. Fragmentation due to land development is a major reason for the declining biodiversity in forest ecosystems. The purposes of this study were (1) to investigate the relationship between the factors of land development and forest fragmentation in 13 watersheds of a metropolitan area and (2) to suggest a forest management plan through the relationship. We carried out a factor analysis to determine explanatory axes of forest fragmentation, and then conducted a correlation analysis between the factor scores and the factor of land development, such as the rate of built-up areas, road density, number of built-up patches, and area of housing developments. The first explanatory axis represented stability of landscape highly related with the rate of the built-up area and road density. The second axis represented the level of fragment highly related with a number of built-up patches. Forest fragmentation patterns of the 13 watersheds were classified for the similarity in forest fragmentation. This study presents the forest management plans including distribution and level of land development and forest conservation.

• Mass Spectrometry Letters
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• 제8권3호
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• pp.65-68
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• 2017

Thiamethoxam is one of the main suspect in honeybee colony collapse disorder (CCD). Due to this reason, thiamethoxam including imidacloprid and clothianidin has been banned for two years in some Europe countries. The CCD phenomenon has also been reported in Korea. Regarding this issue and needs, a new project has started to develop the method for the quatitation of thiamethoxam using isotope dilution mass spectrometry (IDMS). In the process of optimization for the IDMS method with thiamethoxam and $thiamethoxam-d_3$, we observed that the fragment peaks did not correspond to the fragmentation pathway as published elsewhere. Here, we proposed a candidate fragmentation pathway. To validate the proposed fragmentation pathway, another isotope analogue, $thiamethoxam-d_4$, was introduced and the MS/MS spectra of both isotope analogues were compared. In addition, the MS/MS/MS spectra of thiamethoxam were inspected for more evidence of the candidate pathway. Those spectra indicated that the proposed fragmentation pathway could be used to assign the fragment peaks of thiamethoxam.

• 한국실내디자인학회논문집
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• 제23권1호
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• pp.43-51
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• 2014

Over the 30 years of practice, most of David Chipperfield's design projects shows that there are several unique design methods and characteristics applied in the design processes. 'Form Matters' exhibition held in London Design Museum in 2009 was crucial opportunity to summarize his architectural philosophy. The main target of this study is to analyze the Chipperfield's consistent design methods and their meaning based on his architectural philosophy. In chapter 2, his architectural background, early projects and the essence of 'Form Matters' exhibition were studied. In chapter 3, the projects built after 2000 were summarized and main design method that is 'Fragmentation & assemblage' were extracted from them. After selecting 6 case projects, each cases were analyzed in detail based on 3 analytical elements: A) Reason to segment the given volume into smaller volumes, B) Relationship between Fragmentation and Program, C) Relationship between Fragmentation-Assemblage and Interior/Exterior Space. In chapter 5, case analysis were summarized and deep meaning of design method were researched. This study founded that Chipperfield has used Fragmentation-Assemblage method to get 'flexible respond to site conditions', 'appropriate composition of program and space', 'various visual-perceptual experience through intermediary space and materiality'. As a conclusion, his unique design method has a role to logically and aesthetically spatialize all the given contextual situations into a specific architectural 'form'.

• Advanced Composite Materials
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• 제17권1호
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• pp.75-87
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• 2008

Aerospace structural applications, along with high performance marine and automotive applications, require high-strength efficiency, which can be achieved using metal matrix composites (MMCs). Rotating components, such as jet-engine blades and gas turbine parts, require materials that maximize strength efficiency and metallurgical stability at elevated temperatures. Titanium matrix composites (TMCs) are well suited in such applications, since they offer an enhanced resistance to temperature effects as well as corrosion resistance, in addition to optimum strength efficiency. The overall behavior of the composite system largly depends on the properties of the interface between fiber and matrix. Characterization of the fiber.matrix interface at operating temperatures is therefore essential for the developemt of these materials. The fiber fragmentation test shows good reproducibility of results in determining interface properties. This paper deals with the evaluation of fiber fragmentation characteristics in TMCs at elevated temperature and the results are compared with tests at ambient temperature. It was observed that tensile testing at $650^{\circ}C$ of single-fiber TMCs led to limited fiber fragmentation behavior. This indicates that the load transfer from the matrix to the fiber occurs due to interfacial friction, arising predominantly from mechanical clamping of the fiber by radial compressive residual and Poisson stresses. The present work also demonstrates that composite processing conditions can significantly affect the nature of the fiber.matrix interface and the resulting fragmentation of the fiber.

• Mass Spectrometry Letters
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• 제10권2호
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• pp.50-55
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• 2019

The fragmentation statistics of ion trap CID (Collision-Induced Dissociation) spectra using 87,661 tandem mass spectra of doubly charged tryptic peptides are analyzed here. In contrast to the usual method of using intensity information, the frequency of occurrence of fragment ions, with respect to the position of the cleavage site and the residues at these sites is studied in this paper. The analysis shows that the frequency of occurrence of fragment ion peaks is more towards the middle of the peptide than its ends. It was noted that amino acid with an aromatic and basic side chain at N- & C- terminal end of the peptide stimulates more peaks at the lower end of the spectrum. The residue pair effect was shown when the amide bond occurs between acidic and basic residues. The fragmentation at these sites (D/E-H/R/K) stimulates the generation of the y-ion peak. Also, the cleavage site H-H/R/K stimulates the generation of b-ions. K-P environment in the peptide sequence has more tendency to generate y-ions than b-ions. Statistical analysis helps in the visualization of the CID fragmentation pattern. Cleavage pattern along the length of the peptide and the residue pair effects, enhance the knowledge of fragmentation behavior, which is useful for the better interpretation of tandem mass spectra.

• Clinical and Experimental Reproductive Medicine
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• 제46권1호
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• pp.8-13
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• 2019

Objective: Density gradient centrifugation (DGC) is frequently used to isolate high-motility fractions of spermatozoa. We compared the efficacy of four DGC media in terms of the percentage of morphologically normal spermatozoa, DNA fragmentation level, and hyaluronic acid (HA) binding ability. Methods: Thirty men with a total motile spermatozoa count > 80 million participated. Semen samples were divided into four aliquots, which were processed using PureSperm, PureCeption, Sidney, and SpermGrad media, respectively. The DNA fragmentation level was measured using the Halosperm assay kit and HA binding ability was measured using the HBA assay kit. Results: The mean percentage of morphologically normal spermatozoa was significantly enhanced after DGC using all four media (10.3%, 9.9%, 9.8%, and 10.7%, respectively; p< 0.05 for each when compared with 6.9% in raw semen). The DNA fragmentation level was significantly reduced after DGC using PureSperm, PureCeption, and SpermGrad media (6.0%, 6.5%, and 4.9%, respectively; p< 0.05 for each when compared with 11.2% in raw semen), but not after DGC using Sidney media (8.5%, p> 0.05). HA binding ability did not change after DGC using any of the four media. Conclusion: The four media were equally effective for obtaining a sperm fraction with highly motile, morphologically normal sperm. PureSperm, PureCeption, and SpermGrad media were equally effective for acquiring a sperm fraction with less DNA fragmentation.

• 한국터널지하공간학회 논문집
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• 제15권2호
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• pp.81-95
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• 2013

터널 인근에서 폭발이 일어나 붕괴가 발생될 경우 터널의 기능을 회복시키기 위해서는 파쇄영역에 대하여 빠르게 파악하여야 한다. 본 연구에서는 폭발에 따른 거동을 파악하고 파쇄영역을 빠르게 예측할 수 있는 방법을 서술하였다. 이를 위해 SolidWorks를 이용하여 다양한 3차원 요소망을 작성하고, AUTODYN을 이용하여 폭발해석을 수행하였다. 민감도 분석을 실시하여 해석결과를 이용해 폭발위치 등과 같은 폭발변수가 파쇄부피에 미치는 영향을 살펴보았다. 또한 인공신경망 학습자료로 구축하고, 최적의 학습모델을 선정하고, 파쇄부피와 반지름의 예측결과를 검증하였다. 연구결과, 본 연구에서 서술된 방법이 파쇄영역을 빠르고 효과적으로 예측할 수 있음을 확인하였다.