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Protective Effects of Chitosan on the Cadmium Cytotoxicity in Rat Glioma Cells  

백용아 (원광대학교 의과대학)
이정래 (원광대학교 의과대학)
김강득 (원광대학교 의과대학)
김혜원 (원광대학교 의과대학)
이한솔 (원광대학교 의과대학)
허정무 (원광대학교 의과대학)
오재민 (원광대학교 의과대학)
최민규 (원광대학교 의과대학)
정연태 (원광대학교 의과대학)
Publication Information
Toxicological Research / v.20, no.1, 2004 , pp. 63-69 More about this Journal
Abstract
Casapse-3 protease is known as a key role of apoptotic enzyme, and caspase-3 activity is a central event that occurs upstream of DNA fragmentation during apoptosis. This study demonstrates that chitosan pretreatment inhibits cadmium-induced apoptosis by attenuating the activity of caspase-3. We also analyzed the protective effect of chitosan on DNA fragmentation induced by cadmium. Cadmium toxicity was examined by DNA fragmentation and nuclear condensation with Hoechst stain. Caspase-3 activities were increased cadmium treated group for 3 hours compared with control. When chitosan (150 mg/ml) was pretreated at 30 min before cadmium treatment, cadmium cytotoxicity was suppressed in a dose-dependent manner evaluated by DNA fragmentation and caspase activity. From these results, it is suggest that the protective effect of chitosan pretreatment against cadmium-induced cytotoxicity is mediated through inhibition of caspase-3 protease activation and DNA fragmentation.
Keywords
Cadmium; Cytotoxicity; Chitosan; Rat glioma cell;
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