• Title/Summary/Keyword: FM1

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Characteristics of Hemolysin Produced by Vibrio cholerae non-O1 FM-3 Isolated from Sea Water (해수분리 Vibrio cholerae non-O1 FM-3의 Hemolysin)

  • KIM Shin-Hee;PARK Mi-Yeon;LEE Young-Eon;CHO Myo-Heon;CHANG Dong-Suck
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.4
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    • pp.556-561
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    • 1997
  • Vibrio cholerae non-O1 FM-3 was isolated from sea water, and it showed the same bacteriological characteristics as Vibrio cholerae non-O1 ATCC 25872. V. cholerae non-O1 FM-3 presented the highest hemolytic activity at stationary phase of its growth. The hemolytic activity was decreased in accordance with increasing of pretense activity of its cultural supernatant. The characteristics of the hemolysin produced by V. cholerae non-O1 FM-3 were investigated after partial purification with a Sephadex G-100 chromatography. The hemolytic activity of purified protein was stable at $4^{\circ}C$ while it was completely lost by heating at $60^{\circ}C$ for 30 min. The activity of hemolysin was increased by addition of divalent cations such as $Ca^{2+},\;Mg^{2+},\;and\;Mn^{2+}$ while it was inhibited by additions of $Zn^{2+}$. When the hemolysin was incubated with suspensions of erythrocytes at $4^{\circ}C$ and $37^{\circ}C$, respectively, hemolysis was not observed at $4^{\circ}C$ but at $37^{\circ}C$. It means that hemolysis by purified hemolysin was temperature-dependent while its binding step of hemolysin to cell membrane was temperature-independent.

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Effects of Salinity on Chlorophyll Fluorescence from Porphyra Thalli and Comparison of Species with Different Intertidal Distribution

  • Hong Dang Diem;Kim Tae Hoon;Hwang Mi Sook;Chung Ik Kyo;Lee Choon-Hwan
    • Fisheries and Aquatic Sciences
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    • v.1 no.1
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    • pp.122-128
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    • 1998
  • Characteristic changes in chlorophyll fluorescence from thalli of red alga, Porphyra, under high salt stress and during subsequent recovery were investigated, and the differences in the sensitivity to the stress among four species of Porphyra with different intertidal distributions were compared. By the treatment of NaCl with $9\%$ or higher concentrations, photochemical efficiency (Fv/Fm) decreased in a biphasic pattern: a rapid decrease was observed within 1­3 h and followed by a slow decline. The decrease of Fv/Fm was mainly due to the increase of Fo without significant increases of Fm. When the thalli treated with $15\%$ NaCl for 6 h were returned to natural sea water for recovery, the increase of Fv/Fm also showed a biphasic pattern: a rapid increase of Fv/Fm was observed within 2 h and followed by a slow increase. Differences in the sensitivity to salt stress among the four species could be found during recovery after the treatment of severe salt stress. After the treatment of $20\%$ NaCl for 6 h, Fv/Fm decreased below 0.3 in all of the four Porphyra species, and the species living in upper parts of the intertidal zone (P. suborbiculata and P. pseudolinearis) could recover better compared with the species in lower parts of the intertidal zone (P. seriata and P. yezoensis), during recovery for 24 h. The species collected from the coast of the South Sea seemed to be more tolerant than those in the East Sea.

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Propofol Patient-Controlled Sedation Using $Perfusor^{\circledR}$ fm (B. Braun, Germany) Infusion Pump in Dental Patients-Preliminary Study (치과 환자에서의 $Perfusor^{\circledR}$ fm 자가통증조절기를 이용한 Propofol 자가진정조절법)

  • Park, Chang-Joo;Yum, Kwang-Won;Kim, Hyun-Jeong
    • Journal of The Korean Dental Society of Anesthesiology
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    • v.2 no.2 s.3
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    • pp.97-100
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    • 2002
  • Background: Patient-controlled sedation (PCS) has been blown for a safe and effective sedative method on the same pharmacological concepts of patient-controlled analgesia. Many different kinds of infusion devices have been used but they often have too long nominal infusion rate and lockout time. $Perfuser^{\circledR}$ fm (B. Braun, Germany) is a new PCA device with 999.9 ml/hr nominal infusion rate and minimum 1 min lockout time. In this study, the feasibility of propofol PCS using $Perfuser^{\circledR}$ fm was examined in order to provide a safe satisfactory sedation for dental patients. Methods: Eleven healthy patients presenting for oral surgery were studied. Propofol PCS was performed using $Perfuser^{\circledR}$ fm, which was set to deliver a bolus dose of 5 mg with 999.9 ml/hr nominal infusion rate and 1 min lockout time. Propofol loading dose was randomly assigned to a bolus dose ${\times}$ 0, 2, and 3 (initial bolus). Patients were told to press the bolus button as often as they needed to relieve discomfort. Results: Total infused dose of propofol was mean 1.8 mg/kg/hr and D (Delivery)/A (Attempt) ratio was mean 72.8%. All patients was awake and there were no clinically significant intraoperative side effects during the sedation. Almost all patients were very satisfied with this type of PCS. Conclusion: Propofol PCS using $Perfuser^{\circledR}$ fm infusion pump provided good conscious sedation for dental procedures.

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FM Reflectometric Measurement of Group Velocities of Microwave Transmission Lines

  • Park, Yong-Hyun;Lee, Jeong-Hae
    • Journal of electromagnetic engineering and science
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    • v.3 no.1
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    • pp.67-71
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    • 2003
  • In this paper, frequency modulated(FM) reflectometry is proposed to measure group velocity of microwave transmission line Various microwave transmission lines such as periodically loaded conducting posts in a waveguide and nonradiative dielectric(NRD) guide are adopted to measure their group velocity The result compared with that from network analyzer shows good agreement, indicating the validity of our measurement method.

A Study on Low Delay FM Detector for AF Band (AF대용 저지연 FM 검파기에 관한 연구)

  • 김형교;이충웅
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.17 no.6
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    • pp.24-27
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    • 1980
  • 본 논문에서는 J.Klapper와 E.J. Kratt[1]III에 의하여 제안된 저지연 FM검파기의 일반적인 왜곡해석을 Taylor[2] 반수전개법에 의하여 또한 상기의 저지연 FM검파기에서 사용한 RLC Notch 필터를 동 FM 검파기의 IC화를 고려하여 Twin-Tee RC 능동필터로 대치하고 예상되는 검파신호의 지연시간을 검토하였다.

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A Study on Real-time Discrimination of FM Radio Broadcast Speech/Music (실시간 FM 방송중 음악/음성 검출에 관한 연구)

  • 황진만;강동욱;김기두
    • Proceedings of the IEEK Conference
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    • 2003.07e
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    • pp.2136-2139
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    • 2003
  • 본 논문은 FM 라디오 방송중의 오디오 신호를 블록단위로 음악 및 음성을 검출하는 알고리즘에 대한 것으로, 이를 기반으로 방송중의 노래(가요, 팝, 클래식‥‥)만을 자동으로 인식하여 녹음하는 알고리즘을 개발한다. 본 논문에서는 기존에 제안되었던 것[1-4]과 같이 단지 음악과 음성을 구분함과 동시에 음악구간의 논리적 조합으로 이루어진 노래를 자동으로 인식하여 녹음하는 것을 알고리즘의 최종 목표로 한다. 알고리즘의 접근 역시 기존의 음소단위의 모델링을 거치는 GMM 기반의 접근이 아니기 때문에 모델링에 대한 훈련과정이 필요 없고, 시간영역에서의 오디오신호가 가지고 있는 직관적인 특징을 분석함으로써 비교적 적은 연산으로 실시간 구현이 가능하다.

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Effects of Coupling Resistor Resistance Discrepancy from Characteristic Impedance on Discriminating Characteristics of Super Wide-Band FM Line Discriminator (전송선형 초광대역 FM변별기에서 결합 저항기의 오차가 미치는 영향)

  • 이충웅
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.7 no.4
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    • pp.1-5
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    • 1970
  • This presents the way in shich the input impedance, and the output waveform of the line discriminator are influenced by a discrepancy of less than $\pm$10% in the coupling resistor resistance from the optium value(Characteristic impedance of the transmission line used in the line discriminator)

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Utilization of Graded Levels of Finger Millet (Eleusine coracana) in Place of Yellow Maize in Commercial Broiler Chicken Diets

  • Rama Rao, S.V.;Raju, M.V.L.N.;Reddy, M.R.;Panda, A.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.1
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    • pp.80-84
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    • 2005
  • An experiment was conducted to study the performance, carcass traits, serum lipid profile and immune competence in commercial broilers (2 to 42 d of age) fed graded levels (25, 50, 75 and 100%) of finger millet (FM) (Elusine coracana) in place (w/w) of yellow maize (YM). Each diet was fed to eight replicates (five female Vencobb broilers/replicate) housed in stainless steel battery brooders. The estimated metabolizable energy content of FM was about 540 kcal less than the YM. FM contained more protein (10.42 vs. 9.05%) and fibre (9.52 vs. 2.24%) compared to YM. Body weight gain, ready to cook yield, relative weights of giblet, liver, intestine and length of intestine at 42 d of age was not affected due to replacing YM with FM. But, the feed efficiency decreased in broilers fed diets containing 75 and 100% FM in place of YM at both 21 and 42 d of age. The amount of fat deposited in abdominal area decreased and the relative weight of gizzard increased with increase in level of FM in the diet. The serum HDL cholesterol at 21 and 42 d of age and serum triglycerides at 42 d of age decreased with increase in level of FM in diet. The relative weight of spleen and antibody titers against sheep red blood cells (SRBC) at 5 d post inoculation (PI) decreased in broilers fed FM at 100% of YM. However, the relative weight of bursa, SRBC titers at 10 d PI, antibody titers against ND virus and mortality were not affected due to incorporation of FM in place of YM in diet. The fat content in thigh muscle and liver decreased, while the protein content in these tissues increased with increase in the level of FM in broiler diet. Based on the results, it may be concluded that YM can be replaced with FM up to 25% on weight basis without affecting weight gain, carcass yields and immunity in commercial broiler diet (up to 42 d of age). Further, inclusion of finger millet reduced the fat deposition in thigh muscle, liver and in abdominal area compared to those fed maize as the principal source of energy.

Diurnal and Seasonal Variation of Chlorophyll Fluorescence from Korean Fir Plants on Mt. Halla (한라산 구상나무 잎의 엽록소형광의 일변화와 계절적 변화)

  • 오순자;고정군;김응식;오문유;고석찬
    • Korean Journal of Environmental Biology
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    • v.19 no.1
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    • pp.43-48
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    • 2001
  • Chlorophyll fluorescence of needles of Korean fir (Abies koreana) plants and environmental factors of their natural habitat were investigated in order to obtain the information for environmental adaptation and conservation of Korean fir plants. The photochemical efficiency of photosystem II, Fv/Fm, of Korean fir needles was significantly low (0.19-0.36) in the winter, whereas it was high (0.8-0.86) in the summer. The Fv/Fm value of the winter was slightly higher at mid-day than at dawn, suggesting that mid-day environmental conditions of the winter were favorable on needles of Korean fir plants. In contrast, the mid-day Fv/Fm value of the summer maintained high (around 0.8). It indicates that mid-day environmental conditions of the summer did not induce photodamage, although it caused a slight decrease in the Fv/Fm values. The non-photochemical fluorescence quenching (NPQ) of Korean fir needles was very low (0-0.01) all through the day in the winter. However, it was high (0.76) at mid-day in the summer. These results suggest that Korean fir plants have a system for the protection of PS II from mid-day environmental stresses of the summer. In the winter, the Fv/Fm values were positively correlated with temperature, light intensity and relative humidity, although NPQ values showed no correlation with any of them. In the summer, the Fv/Fm values were positively correlated with relative humidity but negatively correlated with temperature and light intensity. These results indicate that increase of tempera-ture, light intensity and relative humidity lead to promotion of the photochemical efficiency in the winter and high temperature and light intensity may cause photoinhibition in the summer.

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Isolation and Characterization of Extracellular Polymeric Substances (EPS)-producing bacteria for restoration of burnt forest soils (산불토양복원을 위한 Extracellular Polymeric Substances (EPS) 생성세균의 분리, 동정 및 특성에 관한 연구)

  • Lee, Gun-Young;Song, In-Geun;Chung, Jae-Chun;Kim, Young-Jun
    • Journal of the Korea Organic Resources Recycling Association
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    • v.12 no.4
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    • pp.139-147
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    • 2004
  • We have isolated two bacterial strains, FM-02 and AL-02, which produced EPS from forest soil for the restoration of forest fire by promoting soil aggregation. FM-02 was found to be Gram negative rod and belong to Beta Proteobacterium sp. through 16s-rDNA sequence analysis, and AL-02 was Gram positive rod and showed 81% of similarity to Zoogloea sp. through the analysis of 16s-rDNA sequence. FM-02 and AL-02 produced about 1.8g and 8.3g of EPS, respectively, per 1L of culture as dry weight. Flocculation activity (FA) was also measured in two strains. FM-02 showed 2.31 FA against active carbon, and AL-02 showed 6.21 FA against kaolin clay. From these results, we expect that AL-02 strain will be applied as a good biological material for the reduction of forest soil erosion by wild and rain after fire through promoting coagulation of soil particles.

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