• Journal of Veterinary Science
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• v.24 no.5
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• pp.67.1-67.15
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• 2023

Background: Feline immunodeficiency virus (FIV) causes an acquired immunodeficiency-like syndrome in cats. FIV is latent. No effective treatment has been developed for treatment the infected cats. The first and second generations non-nucleoside reverse transcriptase inhibitors (NNRTIs) for HIV treatment, nevirapine (NVP) and efavirenz (EFV), and rilpivirine (RPV), were used to investigate the potential of NNRTIs for treatment of FIV infection. Objective: This study aims to use experimental and in silico approaches to investigate the potential of NNRTIs, NVP, EFV, and RPV, for inhibition of FIV reverse transcriptase (FIV-RT). Methods: The FIV-RT and human immunodeficiency virus reverse transcriptase (HIV-RT) were expressed and purified using chromatography approaches. The purified proteins were used to determine the IC₅₀ values with NVP, EFV, and RPV. Surface plasmon resonance (SPR) analysis was used to calculate the binding affinities of NNRTIs to HIV-RT and FIV-RT. The molecular docking and molecular dynamic simulations were used to demonstrate the mechanism of FIV-RT and HIV-RT with first and second generation NNRTI complexes. Results: The IC₅₀ values of NNRTIs NVP, EFV, and RPV against FIV-RT were in comparable ranges to HIV-RT. The SPR analysis showed that NVP, EFV, and RPV could bind to both enzymes. Computational calculation also supports that these NNRTIs can bind with both FIV-RT and HIV-RT. Conclusions: Our results suggest the first and second generation NNRTIs (NVP, EFV, and RPV) could inhibit both FIV-RT and HIV-RT.

• Journal of Veterinary Clinics
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• v.22 no.1
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• pp.1-5
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• 2005

Seroprevalence of feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) infections were surveyed in 875 domestic cats derived from 4 major cities and 4 provinces in Korea. Of those tested, 715 were healthy and 160 were sick. FeLV and FIV were tested with AGEN/sup ⓡ/ FeLV-FIV, a commercial combo test kit. Ten out of 875 cats (1.1 %) were seropositive for FeLV and none out of 875 cats (0%) was positive for FIV, respectively. The positive rates for FeLV were 3.8% in sick cats and 0.6% in healthy cats. The cats at the age of more than 1 year showed higher incidence rates than the younger ones. There were no significant relationships either with the environment or with the sex. The prevalence of seropositive FeLV was 0.7% in mixed cats and 1.6% in pedigree cats. With respect to the regional prevalence of the FeLV, the highest seroprevalence (9.5%) was found in Gyeongsang province.

• Reproductive and Developmental Biology
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• v.31 no.3
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• pp.153-159
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• 2007

In this study, using FIV-based lentivirus vector system, we tried to express hG-CSF in tetracycline-controllable manner. hG-CSF influences the proliferation, differentiation, and survival of cells in the neutrophil lineage. To enhance stability and translation of hG-CSF transcript, WPRE sequence was also introduced into FIV-Tet-On vector at downstream region of either the hG-CSF gene or the sequence encoding rtTA. Primary culture cells (CEF, chicken embryonic fibroblast; PFF, procine fetal fibroblast) infected with the recombinant FIV were cultured in the medium supplemented with or without doxycycline for 48 hours, and induction efficiency was measured by comparing the hG-CSF gene expression level using quantitative real-time PCR, Western blot and ELISA. Higher hG-CSF expression and tighter expression control were observed from the vector in which the WPRE sequence was placed at downstream of the hG-CSF (in CEF) or rtTA (in PEE) gene. This FIV-Tet-On vector system may be helpful in solving serious physiological disturbance problems which has continuously hampered successful production of transgenic animals and gene therapy.

• Journal of the Korean Society of Food Science and Nutrition
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• v.7 no.2
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• pp.7-12
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• 1978

This experiment was conducted to fractionate the trypsin inhibitors and its heat stability of five varieties of soybean. It was observed that water extractable protein of all varieties used was fractionated into three peaks and the second peak seemed to show trypsin inhibitor activity. (TIA). The trypsin inhibitors were fractionated into four fractions-FI, FII, FIII and FIV. FIII showed the highest TIA in Suwon No. 81, Suwon No. 82 and Suwon No. 83 In the case of Kyungnam No. 3 and Suwon No. 62, the highest activity was found in FIV. When trypsin inbibitors fractionated in boiling water-bath for 20 min, the FIV showed the highest heat stability and FI was found to be the weakest.

• Proceedings of the KSME Conference
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• 2004.04a
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• pp.905-910
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• 2004

Vibration characteristics of the FIV test loop for the Flow-Induced Vibration(FIV) study of a PWR partial(5x5) fuel assembly are investigated by the Finite Element(FE) analysis and the modal test. For the FE analysis, 3-D beam element is used for the pipes and the test section and mass element used for the valves and flanges. The 'U' restrainer stiffness determined by numerical simulation is used for the FE model. The result of the FE analysis is compared with that of the modal test. The higher mode similarity between the test and analysis is observed in a few low modes. After that, the mode similarity reduce as the mode goes high. It is concluded that the first to the third vibration modes are observed at the lower parts of the 6 inches restoring line, followed by a local mode at the test section, and the natural frequencies of the modes are 22.4 Hz, 26.0 Hz, 27.5 Hz and 31.4 Hz.

• Journal of Mechanical Science and Technology
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• v.18 no.1
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• pp.74-81
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• 2004

The KSNP Steam Generators (Youngkwang Unit 3 and 4, Ulchin Unit 3 and 4) have a problem of U-tube fretting wear due to Flow Induced Vibration (FIV). In particular, the wear is localized and concentrated in a small area of upper part of U-bend in the Central Cavity region. The region has some conditions susceptible to the FIV, which are high flow velocity, high void fraction, and long unsupported span. Even though the FIV could be occurred by many mechanisms, the main mechanism would be fluid-elastic instability, or turbulent excitation. To remedy the problem, Eggcrate Flow Distribution Plate (EFDP) was installed in the Central Cavity region or Ulchin Unit 5 and 6 steam generators, so that it reduces the flow velocity in the region to a certain level. However, the cause of the FIV and the effectiveness of the EFDP was not thoroughly studied and checked. In this study, therefore the Stability Ratio (SR), which is the ratio of the actual velocity to the critical velocity, was compared between the value before the installation of EFDP and that after. Also the possibility of fluid-elastic instability of KSNP steam generator and the effectiveness of EFDP were checked based on the ATHOS3 code calculation and the Pettigrew's experimental results. The calculated results were plotted in a fluid-elastic instability criteria-diagram (Pettigrew, 1998, Fig. 9). The plotted result showed that KSNP steam generator with EFDP had the margin of Fluid-Elastic Instability by almost 25％.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.33 no.10
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• pp.1108-1118
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• 2009

A Very High Temperature Gas Cooled Reactor (VHTR) has been selected as a high energy heat source of the order of $950^{\circ}C$ for nuclear hydrogen generation, which can produce hydrogen from water or natural gas. A primary hot gas duct (HGD) as a coaxial double-tube type cross vessel is a key component connecting a reactor pressure vessel and an intermediate heat exchanger in the VHTR. In this study, a structural sizing methodology for the primary HGD of the VHTR is suggested in order to modulate a flow-induced vibration (FIV). And as an example, a structural sizing of the horizontal HGD with a coaxial double-tube structure was carried out using the suggested method. These activities include a decision of the geometric dimensions, a selection of the material, and an evaluation of the strength of the coaxial double-tube type cross vessel components. Also in order to compare the FIV characteristics of the proposed design cases, a fluid-structure interaction (FSI) analysis was carried out using the ADINA code.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2009.04a
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• pp.652-653
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• 2009

In this study, flow-induced vibration (FIV) analyses have been conducted for a 3D compressor blade model. Advanced computational analysis system based on computational fluid dynamics (CFD) and computational structural dynamics (CSD) has been developed in order to investigate detailed dynamic responses of designed compressor blades. Fluid domains are modeled using the computational grid system with local grid deforming and remeshing techniques. Reynolds-averaged Navier-Stokes equations with $\kappa-\varepsilon$ turbulence model are solved for unsteady flow problems of the rotating compressor model. A fully implicit time marching scheme based on the Newmark direct integration method is used for computing the coupled aeroelastic governing equations of the 3D compressor blade for fluid-structure interaction (FSI) problems. Detailed dynamic responses and instantaneous pressure contours on the blade surfaces considering flow-separation effects are presented to show the multi-physical phenomenon of the rotating compressor blade.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.19 no.6
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• pp.551-559
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• 2009

In this study, flow-induced vibration(FIV) analyses have been conducted for a 3D compressor blade model. Advanced computational analysis system based on computational fluid dynamics(CFD) and computational structural dynamics(CSD) has been developed in order to investigate detailed dynamic responses of designed compressor blades. Fluid domains are modeled using the computational grid system with local grid deforming and remeshing techniques. Reynolds-averaged Navier-Stokes equations with $\kappa-\epsilon$ turbulence model are solved for unsteady flow problems of the rotating compressor model. A fully implicit time marching scheme based on the Newmark direct integration method is used for computing the coupled aeroelastic governing equations of the 3D compressor blade for fluid-structure interaction(FSI) problems. Detailed dynamic responses and instantaneous pressure contours on the blade surfaces considering flow-separation effects are presented to show the multi-physical phenomenon of the rotating compressor blade.

• Nuclear Engineering and Technology
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• v.33 no.6
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• pp.619-625
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• 2001

An axial-flow-induced vibration model was proposed for a rod supported by two translational springs at both ends. For developing the model, a one-mode approximation was made based on the assumption that the first mode was dominant in vibration behavior of the single span rod. The first natural frequency and mode shape functions for the flow-induced vibration, called the FIV model were derived by using Lagrange＇s method. The vibration displacements at reactor conditions were calculated by the proposed model for the spring-supported rod and by the previous model for the simple-supported(55) rod. As a result, the vibration displacement for the spring-supported rod was larger than that of the 55 rod, and the discrepancy between both displacements became much larger as flow velocity increased. The vibration displacement for the spring-supported rod appeared to decrease with the increase of the spring constant. AS flow velocity increased, the increase rate of vibration displacement was calculated to go linearly up, and that of the rod having the short span length was larger than that of the rod having the long span length although the displacement value itself of the long span rod was larger than that of the short one.