• 한국수정란이식학회지
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• 제10권2호
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• pp.115-120
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• 1995

This study was carried out to investigate On in vitro fertilization, survival rate and developmental rate of rapidly frozen bovine immature oocytes. Immature oocytes cultured for 1, 12, 24, 48 hours in 20% FCS + TCM-199 medium and thereafter rapidly freezing-thawed oocytes inseminated with capacitated sperm. The immature oocytes following dehydration by 1.5M DMSO + 2.0M glycerol + 0.25M sucrose + TCM 199 media + 20% FGS were directly plunged into liquid nitrogen and thawes in 3$0^{\circ}C$ water. Rapid freezing embryos co-cultured in 20% FCS + TCM-199 media containing hormones(21U/mL PMSG, 21U /mL hGG and 1 $\mu$g /mL 17$\beta$-estradiol) and cumulus cells(1 x 105-6 cells). Survival rate was defined as development rate on in vitro culture or FDA-test. The results are summarized as follows ; 1. The in vitro maturation and fertilization rate of immature bovine oocytes on in vitro maturation period(1, 12, 24, 48 hrs) before rapid freezing4hawed were 57.1%, 45.7%, 37.1%, 25.7% and 40.0%, 31.4%, 20.0%, 11.4%, respectively. 2. The survival rate of immature bovine oocytes on in vitro maturation period(1, 12, 24, 48 hrs) before rapid freezing-thawed were 33.3%, 26.7%, 20.0%, and 10.0%, respectively. The survival rate of rapid freezing4hawed immature oocytes was significantly lower than that of non-freezing oocytes. 3. The survival rate of rapid freezing4hawed excellent and good bovine embryos co-cultured in 20% FCS + TCM-199 media containing hormones(PMSG, hCG, 17$\beta$-estradiol) and cumulus cells 4 to 5 hrs and 20 to 24 hrs were 35.0%, 15.0% and 25.0%, 15.0% and 40.0%, 20.0% and 30.0%, 15.0%, respectively. The survival rate of embryos co-cultured in TCM-199 media containing hormones and cumulus cells was significantly higher than that of non co-culture.

• Journal of Advanced Marine Engineering and Technology
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• 제40권9호
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• pp.780-785
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• 2016

선박의 연료로 이용되는 중유는 지구 온난화를 일으키는 유해가스를 배출한다. 이를 저감하기 위해 친환경선박으로 표현되고 있는 녹색선박이 등장하게 되었고, 천연가스를 연료로 하는 액화천연가스(LNG) 연료 선박이 대표적인 사례이다. 본 논문에서는 LNG 선박에 사용되는 극저온 환경에서 고압 하중을 받는 기화기와 출입구 배관의 구조 건전성을 ASME 코드에 따라 평가하고 실용화한 최초의 사례이다. 기화기와 배관은 유한요소법을 사용하여 구조해석을 수행하였다. 기화기는 등가응력을 바탕으로 ASME Section VIII Division 2에 제시된 허용응력과 비교하여 건전성 평가를 수행하였고, 배관은 성분별 응력을 조합하여 ASME B31.3에 제시된 허용응력과 비교 및 건전성 평가를 수행하였다. 각 하중에 대한 구조물들의 응력 결과는 허용응력 범위 이내에 있으므로 구조적 건전성을 유지하는 것으로 평가되었다.

• Steel and Composite Structures
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• 제39권5호
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• pp.615-630
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• 2021

The main purpose of the present work was to study the dynamic instability of a three-layered, thick composite sandwich beam with the functionally graded (FG) flexible core subjected to an axial compressive follower force. Flutter instability of a sandwich cantilever beam was analyzed using the high-order theory of sandwich beams, for the first time. The governing equations in general for sandwich beams with an FG core were extracted and could be used for all types of sandwich beams with any types of face sheets and cores. A polynomial function is considered for the vertical distribution of the displacement field in the core layer along the thickness, based on the results of the first Frosting's higher order model. The governing partial differential equations and the equations of boundary conditions of the dynamic system are derived using Hamilton's principle. By applying the boundary conditions and numerical solution methods of squares quadrature, the beam flutter phenomenon is studied. In addition, the effects of different geometrical and material parameters on the flutter threshold were investigated. The results showed that the responses of the dynamic instability of the system were influenced by the follower force, the coefficients of FGs and the geometrical parameters like the core thickness. Comparison of the present results with the published results in the literature for the special case confirmed the accuracy of the proposed theory. The results showed that the follower force of the flutter phenomenon threshold for long beams tends to the corresponding results in the Timoshenko beam.

• 한국식생활문화학회지
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• 제25권6호
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• pp.652-660
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• 2010

The aim of this study was to develop a KDPS (Korean dietary pattern score) to assess dietary patterns and diet quality of Koreans from a food culture perspective. The KDPS was applied to dietary data collected during the Korean National Health and Nutrition Examination Survey of 2007, and the validity and reliability of the KDPS were evaluated. The targets of the study included 2,278 Korean adults aged 20n89 years. The KDPS was developed using the sum of the scores of 13 components. Each component scored up to 10 points and there was a total of 130 points. The first seven components were for the KSMS (Korean-style meal score) and assessed the dietary balance based on the 3-Chup Bansang daily basic table setting. The components numbered 8 to 13 were for the FGS (food group score), which measured the degree of compliance with the six major food groups based on the Korean recommendation for one serving size of grains, meats, vegetables, fruits, milk, and oils. This KDPS was verified through content validity, concurrent-criterion validity, principal components analysis, and a reliability analysis. The results showed that content validity and construct validity were high. The KDPS developed in this study adhered to the Korean dietary pattern and a healthy diet intake. Furthermore, this study presented an integrated index by scoring the Korean style table setting in addition to evaluating meals from a nutrition perspective. This study can be extended to develop a score for assessing.

• 대한간호학회지
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• 제27권3호
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• pp.563-576
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• 1997

This study was carried out to identify basic data for more efficient operation of comprehensive health check-up centers in the national health care system. The researcher reviewed and analyzed the main symptoms of the subjects according to sex, age, occupation and positive rate in the screening tests of a comprehensive health check-up, performed at one comprehensive health check-up center in located in a hospital. The subjects were 512 persons who had a check-up at the comprehensive health check-up, center in K university hospital in Seoul from October 2, 1996 to March 30, 1997. A questionaire developed by the researcher to obtain subject's general characteristics, main symptoms, medical examination, diagnosed disease was used as the tool for the study. The researcher analyzed the data with SPSS PC/sup +/ program. : the distribution of the subject's general characteristics and main symptoms was described with percentages and the difference between main symptoms and screening tests analyzed with X²-test, t-test, ANOVA. The result of this study are as follows : 1. For the distribution of diagnosis in males. liver disease was most frequent and hypertension was second : in females, hypertention and liver disease were not common in that order. 2. The most common complaints were indigestion in the digestive system, sputum in the respiratory system, irregular heart beat in the cardiovascular system, frequent urine in the urinary system vertigo in the hematologic system. 3. The main symptoms according to sex were for females, a statistically siguificant higher rate of complaints in the digestive system, cardiovascular system, urinary system, hematologic system, musculoskeletal, mental & nervous system as compared to males. 4. The main symptoms according to age were that in the the second decade there wara higher rate of complaints in the digestive system, and cardiovascular system than in the older age groups : and in the fifth decade more in the musculoskeletal system. 5. The main symptoms according to occupation were that formers and fishers had a higher rate of complaints in the mental and nervous system as compared to other occupation groups : and office workers, in gynecology. 6. The main symptoms according to diagnosis, were symptoms of the digestive system, frequent in liver disease : symptoms of the cardiovascular system in allergic disease. 7. The screening tests which showed the highest rates were UGI /FGS, PFT : In males a higher rate of abnormality was seen in abdominal ultrasono- graphy and in, females, in mammography. 8. In screening tests according to sex, males showed a higher positive rate for chest X-rays, and LFT, and females, in mammography, pap smear, CBC. 9. In screening tests according to age, higher positive rates of obesity, high blood pressure, chest X-ray, mammography, pap smear, FBS, CBC, urine test were seen in the fifth decade : PFT and abdominal ultrasonography in the third decade. The subjects who wanted comprehensive health check-up were not asymptomatic but had an illness with several symptoms. It is found that the most frequent diagnosed diseases were hypertention and liver disease. So proper observations must be done for them. Comprehensive health check-ups should be changed to a comprehensive examination including treatment beyond examination for those subjects with positive results.