• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.321-324
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• 2001

R. eutropha 의 PHA 생합성 유전자를 포함하는 플라스미드 pSYLl07을 가진 재조합 대장균 GCSC6576 과 A. latus PHA 생합성 유전자를 포함하는 플라스미드 pJC4 한 가진 fadR atoC 돌연변이주 재조합 대장균 LS5218 의 유청으로부터 P(3HB- co -3HV) 합성을 비교하였다. 재조합 대장균 LS5218의 pH-stat 유가식 배양결괴 39 시간에 균체농도 31.8 g/L. P(3HB-co-3HV) 농도 10.6 g/L. P(3HB-co-3HV) 함량 33.4 wt%. 3HV 함량 6.26 mol%를 얻을 수 있었다.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 1996년도 정기총회 및 학술발표회
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• pp.45-45
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• 1996

Flavin-peptides were purified from pyranose oxidase (EC 1.1.3.10) after tryptic- chymotryptic and tryptic digestion. The spectral and chromatographic properties of these flavin peptides showed that the FAD of pyranose oxidase appears to be bound, by way of the 8${\alpha}$-methylene group, to the N-l position of the imidazole ring of the histidine. Automated sequence analysis showed that the amino acid sequence of the tryptic-chymotryptic flavin-peptide from pyranose oxidase is Ser-Thr-X-Trp and that of the tryptic flavin-peptide is Met-Ser-Thr-X-Trp. (omitted)

• 패션비즈니스
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• 제1권3호
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• pp.19-34
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• 1997

The role of fashion image in modern society is being diversified from product planning to consumer satisfaction. In this research, after surveying female college students, the diversified modern fashion image was patterned. On the analogy of the relationship between these patterned image and design factors, the following conclusion was obtained. 1. The most popular styles are jacket and pants suits, three-piece outfits, tight mini-skirts and X-silhouette. Little decoration is used in general. simplicity is shown as trend by utility fabrics in single color-black, brown, beige and blue-with no pattern. Various materials such as wool, cotton, wool or cotton blended with other materials, silk, satin, knit, lace, vinyl and leather are used. 2. Fashion image is divided into four factors; factors that show fad, dignity, activity and simplicity. 3. Factors that show fad; style of tops, material of bottoms and length of bottoms reflect fad. blousons, three-piece outfits, pants rather than skirts, X-silhouette and mini-skirts are prevailing. Vinyl, leather, knit, lace and satin are the materials well used. 4. Factors that show dignity; Style and material of tops, the way of top and bottom pieces are matched and length of bottom or pieces reveal the characteristics. Jackets, blouses, half-coats and one-piece outfits rather than two-pieces are popular. Tight skirts in natural or midi length or pleated skirts are common. Wool, wool blended with other materials, leather and X-silhouette are design factors that reveal dignity well. 5. Design factors that show active image; Straight pants, three-piece outfits, T-shirts, shirt -blouses, vests, and blausons are popular. Knit and lace are used for tops, leather and vinyl are used for bottom pieces and cotton is widely used. Y- or H-silhouette are used to show active image.

• 한국식품영양학회지
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• 제30권3호
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• pp.473-481
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• 2017

The purpose of this study was to investigate the fad dieting status and needs of nutrition education on healthy dieting in male and female collegians according to the desired loss weight. The subjects were 611 students (287 males, 324 females) from universities in Gyeonggi and Gangwon provinces in Korea. The subjects were divided into the following four groups based on the difference between their current body weight and their desired body weight: no desired weight loss (WL0), desired weight loss less than 5 kg (WL5), desired weight loss 6 to 10 kg (WL10), and desired weight loss more than 11 kg (WL11). The fad dieting status was surveyed using a questionnaire about 11 selected trendy dieting methods. The higher desired weight loss group showed higher current weight and BMI in both males and females. Self weight overestimation was higher in the females than in the males, and in the WL10 group than in the other groups of males. As desired loss weight increased, weight loss trials and experienced trendy dieting types increased in both males and females. In the male subjects, the experience of the half meal diet and the chicken breast diet was significantly associated with the desired loss weight. In females, trials of more trendy diet types including the half meal diet, cereal diet, fasting, and Atkins diet were significantly associated with the higher desired loss weight. The need for nutrition education on healthy dieting was high among all the different desired weight loss groups, showing that a minimum of 60% of subjects in each group answered 'necessary and strongly necessary', with the highest in the WL11 group in both males and females. From these results, desired loss weight is associated with higher current weight status, unhealthy weight control practices, and needs for nutrition education in both male and female subjects. Therefore, in the future, nutrition education programs should give greater attention to providing healthy dieting methods for young collegians who desire more weight loss.

• 대한수의학회지
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• 제51권3호
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• pp.209-216
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• 2011

Four strains of fowl adenovirus (FAdV) were isolated from 4 flocks of broiler or layer chickens affected by hydropericardium syndrome in Korea. These FAdVs were classified as serotype 4 by restriction fragment length polymorphism patterns of hexon genes and whole genomes. The virus exhibited cytopathic effects consisting of rounding, ballooning and clustering in primary chicken embryo liver cell cultures. In transmission electron microscopy, virus particles in hexagonal shape aggregated exclusively in the nuclei of hepatocytes of the chickens as the typical appearances in adenovirus infections. Buoyant density of the virus in cesium chloride (CsCl) was 1.34 g/mL. The virus was stable to chloroform, ether, 50~70% ethanol, acidic condition at pH 3, 0.25% trypsin (1 : 250), heat at $50^{\circ}C$ for 30 min, but labile to 100% ethanol, heat at $52{\sim}60^{\circ}C$ for 30 min, 1 M $MgCl_2$ at $50^{\circ}C$ for 1 h, 1 : 2,000 formalin (37%). All of the physicochemical properties pertained to the characteristics of adenoviruses. Eight viral polypeptides were determined in CsCl-purified virus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

• Bulletin of the Korean Chemical Society
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• 제30권12호
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• pp.2984-2988
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• 2009

A gene (PH0311) encoding a hypothetical protein from the genome sequence data of the hyperthermophilic archaeon Pyrococcus horikoshii OT3 was cloned and over-expressed in Escherichia coli. The purified recombinant protein was found to possess FAD-dependent NADH oxidase activity, although it lacked sequence homology to any other known general NADH oxidase family. The product of the PH0311 gene was thus designated PhNOX (NADH oxidase from Pyrococcus horikoshii), with an estimated molecular weight of 84 kDa by gel filtration and 22 kDa by SDS-PAGE, indicating it to be a homotetramer of 22 kDa subunits. PhNOX catalyzed the oxidation of reduced ${\beta}$-NADH with subsequent formation of $H_2O_2$ in the presence of FAD as a cofactor, but not ${\alpha}$-NADH, ${\alpha}$-NADPH, or ${\beta}$-NADPH. PhNOX showed high affinity for ${\beta}$-NADH with a Km value of 3.70 ${\mu}$M and exhibited optimum activity at pH 8.0 and 95$^{\circ}C$ as it is highly stable against high temperature.

• Bulletin of the Korean Chemical Society
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• 제25권5호
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• pp.721-725
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• 2004

Acetohydroxy acid synthase (AHAS) is one of several enzymes that require thiamine diphosphate and a divalent cation as essential cofactors. The active site contains several conserved ionizable groups and all of these appear to be important as judged by the fact that mutation diminishes or abolishes catalytic activity. Recently, we have shown [Yoon, M.-Y., Hwang, J.-H., Choi, M.-K., Baek, D.-K., Kim, J., Kim, Y.-T., Choi, J.-D. FEBS Letters 555 (2003), 185-191] that the activity is pH-dependent due to changes in $V_{max}$ and V/$K_m$. Data were consistent with a mechanism in which substrate was selectively catalyzed by the enzyme with an unprotonated base having a pK 6.48, and a protonated group having a pK of 8.25 for catalysis. Here, we have in detail studied the pH dependence of the kinetic parameters of the cofactors (ThDP, FAD, $Mg^{2+}$) in order to obtain information about the chemical mechanism in the active site. The $V_{max}$ of kinetic parameters for all cofactors was pH-dependent on the basic side. The pK of ThDP, FAD and $Mg^{2+}$ was 9.5, 9.3 and 10.1, respectively. The V/$K_m$ of kinetic parameters for all cofactors was pH-dependent on the acidic and on the basic side. The pK of ThDP, FAD and $Mg^{2+}$ was 6.2-6.4 on the acidic side and 9.0-9.1 on the basic side. The well-conserved histidine mutant (H392) did not affect the pH-dependence of the kinetic parameters. The data are discussed in terms of the acid-base chemical mechanism.

• 재무관리연구
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• 제27권1호
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• pp.121-156
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• 2010

본 연구에서는 2003년~2005년 기간에 코스닥 시장에서 발생한 두 가지 자본시장 진입수단인 IPO와 우회상장을 대상으로 벤치마킹 대비 단기 및 장기 초과보유수익률을 분석하고, 차이점과 의미에 대해 논하였다. 우회상장은 M&A의 성격을 띠고 있지만 동시에 IPO의 성격도 갖고 있다. IPO는 거래소가 제시한 상장요건을 맞춰야 하는 등 제도적인 간섭을 받기 때문에 제도에 의한 자본시장 진입방법이라고 할 수 있다. 반면 우회상장은 이러한 제도의 간섭은 미미하고 당사자 간의 거래에 의하여 진입여부가 결정되기 때문에 거래에 의한 자본시장 진입방법이라고 할 수 있다. 제도가 완전시장을 저해한다는 상식에 기초한다면우회상장이 IPO보다 우수한 진입방법이라고 판단할 수 있지만, 반대로 IPO는 우회상장에 비해 정보의 비대칭성을 줄이고 부실한 기업의 시장진입을 통제할 수 있다는 면에서 장점이 있다고 볼 수 있다. 본 연구의 결과는 정보의 효율성 면에서 IPO가 우수하다는 본문의 주장을 지지하고 있다. 먼저 IPO 기업과 우회상장 기업은 모두 초기에는 높은 초과수익률을 기록하고 장기적으로는 둘 다 저성과를 보이나 우회상장 기업의 장기성과가 더욱 하락하였다. 또한 두 표본 모두 사건 이후 3년 간의 회계적 성과가 하락하였으나 특히 우회상장 기업은 더욱 하락하였으며 모든 기간에서 손실을 기록하였다. 이는 과잉수요(fad)가 코스닥 시장의 IPO는 물론 우회상장에서도 똑같이 존재하며, 우회상장의 경우에는 과대포장(window dressing)의 가능성이 더욱 높음을 뜻한다. 결국 IPO보다는 우회상장이 상장시점에서 회사와 투자자 사이에 더욱 심한 정보의 비대칭성을 보이고 있음을 시사한다.

• KSBB Journal
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• 제18권5호
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• pp.404-407
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• 2003

R. eutropha의 PHA 생합성 유전자를 포함하는 플라스미드 pSYL107을 가진 재조합 대장균 GCSC6576과 A. latus PHA 생합성 유전자를 포함하는 플라스미드 pJC4를 가진 fadR atoC 돌연변이주 재조합 대장균 LS5218의 유청으로부터 P(3HB-co-3HV) 합성을 비교하였다. 재조합 대장균 GCSC6576(pSYL107)의 플라스크 배양에서 acetic acid induction과 oleic acid의 첨가는 3HV 함량을 증가시켰다. 재조합 대장균 LS5218의 pH-stat 유가식 배양결과, 39시간에 균체농도 31.8 g/L, P(3HB-co-3HV) 농도 10.6 g/L, P(3HB-co-3HV) 함량 33.4%, 3HV 함량 6.26 mol%를 얻을 수 있었다.

• 생명과학회지
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• 제28권6호
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• pp.656-662
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• 2018

향상된 인디고이드 생산능력을 갖는 코리네박테리움 유래의 변이 플라빈-함유 모노옥시게나제(cFMO)를 개발하기 위하여, cFMO 효소의 상동성모델을 이용하여 말토오스-결합단백질(MBP)과 융합된 4가지 변이체(F170Y, A210G, A210S, T326S)를 제작하고 그 생화학적 특징을 밝혔다. 정제된 MBP-T326S는 최적 활성을 위하여 야생형보다 고농도의 FAD ($100{\mu}M$)를 요구하며, $100{\mu}M$의 FAD 첨가조건에서 $k_{cat}/K_m$이 3.8배 증가되었다. 인돌 옥시게나제 활성은 야생형의 63-77%를 나타냈다. MBP-T326S는 기질이 존재하지 않을 경우 쓸모없는 NADPH 산화효소 활성이 매우 낮은 수준을 보여주었다(21-24%). T326S이외의 변이 단백질들은 야생형에 비하여 $K_m$은 비슷하며 $k_{cat}/K_m$은 증가하였다. MBP-F170Y와 -A210S 변이단백질은 인돌 옥시게나제 활성이 각각 3.1배, 2.9배 증가하였다. 2.5 g/l의 트립토판을 함유한 LB배지에서 인디고이드 생산을 시험했을 때, 야생형 cFMO를 함유한 대장균은 684 mg/l의 인디고와 104 mg/l의 인디루빈을 생산한 반면, T326S를 함유한 세포는 1,040 mg/l의 인디고와 112 mg/l의 인디루빈을 생산하였다. 이전의 결과인 Methylophaga 유래의 FMO를 발현하는 대장균에서 가장 높은 수준인 920 mg/l의 인디고를 생산한 것과 비교하면, 본 연구결과는 인디고 생산이 13% 높은 수준이였다. 상동성 모델링에 기반한 cFMO의 단백질공학은 인디고이드 생산균을 개발하는데 보다 더 논리적인 전략을 제시하였다.