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Synthesis of Poly[3-hydroxybutyrate-co-3-hydroxyvalerate] by Recombinant Escherichia coli from Whey  

김범수 (충북대학교 화학공학부)
이상엽 (한국과학기술원 생명화학공학과)
Publication Information
KSBB Journal / v.18, no.5, 2003 , pp. 404-407 More about this Journal
Abstract
Two recombinant Escherichia coli strains, GCSC6576 harboring a plasmid pSYL107 containing the Ralstonia eutropha polyhydroxyalkanoate (PHA) biosynthesis genes and a fadR atoC mutant LS5218 harboring a plasmid pJC4 containing the Alcaligenes latus PHA biosynthesis genes were compared for their ability to synthesize poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV) from whey. The 3HV fraction could be increased by acetic acid induction and oleic acid supplementation in flask cultures of recombinant E. coli GCSC6576. With the pH-stat fed-batch culture of recombinant E. coli LS5218, we obtained a cell concentration, a P(3HB-co-3HV) concentration, a P(3HB-co-3HV) content, and a 3HV fraction of 31.8 g/L, 10.6 g/L, 33.4%, and 6.26 mol%, respectively in 39 h.
Keywords
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate); recombinant Escherichia coli; whey; fed-batch culture;
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