• Title/Summary/Keyword: F2 generation

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Selection Efficiency of Resistant Tobacco Plants to Bacterial wilt Disease Using Two Haploid Methods (반수체 육종법을 이용한 잎담배 세균성마름병 저항성 개체 선발의 효율성 비교)

  • 정윤화
    • Journal of the Korean Society of Tobacco Science
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    • v.15 no.2
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    • pp.137-144
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    • 1993
  • The present study was conducted to compare the efficiency of individual plant selection for resistance to bacterial wilt with 30 dihaploid lines derived by anther culture and Nicotiana africana method in Fl and F2 generation from a cross between Bright Yellow 4 (BY4) and NC95. F2 dihaploid lines were selected from bacterial wilt disease resistant plants screened under the naturally infested filed conditions. The populations of FB - ADH and FB MDH derived from F2 individual plants with bacterial wilt resistance showed higher resistance to the disease than the populations of Fl - ADH and Fl - MDH, respectively, and no difference for the disease resistance appeared by the haploid deriving method within a generation.

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Inheritance of Resistance to Phytophthora capsici by Inoculums in Korean Hot Pepper (고추 역병균의 접종원에 따른 역병 저항성의 유전 양식)

  • Soh, Jaewoo;Han, Kyung-Sook;Lee, Sung-Chan;Lee, Jung-Sup
    • Research in Plant Disease
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    • v.18 no.4
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    • pp.317-323
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    • 2012
  • The study aims to identify the pathogenicity of Phytophthora. capsici isolates in major pepper-producing areas in Korea and the inherit genetic pattern of phytophthora blight resistance by inocula. With five kinds of testing materials including 'Kataguma (Sakata Korea)' peppers, a disease-susceptible material, '#308', a phytophthora blight resistance material, 'CM334', and their $F_1$ and $F_2$, respective isolates of P. capsici obtained from Icheon, Eumseong, Buan, Imsil and Yeongyang regions together with six kinds of peppers' inoculum including PA-159 (KACC No.40482) received from Korean Agricultural Culture Collection were used for inoculation. The disease-susceptible material '#308', the resistant material 'CM334' and the non-segregating generation of $F_1$ represented 4.94-5.00, 1.00-1.07, and 1.01-1.08 phytophthora blight incidence respectively in the group comparison by isolate. This result means that the phytophthora blight resistance was clearly distinguished among testing materials in the group comparison by P. capsici isolate. Moreover, $F_2$ segregating generation showed 1.79-2.31 phytophthora blight incidence which turned out to be identical in the group comparison by the six isolates of P. capsici isolate and with similarity between both the resistant and susceptible materials. Thus, the result proved that using the six isolates of P. capsici tested as inocula was suitable to investigate the phytophthora blight resistance. When it comes to group comparison of $F_2$ segregation generation, however, isolates were divided with PA-159 isolate being the center: a group consisting of isolates from Icheon, Buan, and Imsil and a group consisting of Yeongyang and Eumseong isolates with higher pathogenicity. The expected segregation ratio of the phytophthora blight resistance in $F_2$ generation by isolate was analyzed. PA-159 isolate showed 3:1 or 9:3:3:1, indicating that one to two genes were involved. On the other hand, results also proved that there is an interaction of genes since both Eumseong and Yeongyang isolates showed a segregation ratio of 11:5 while the Icheon isolate represented 12:3:1.

[ $F_2$] Production of Gynogenetic Diploid in Olive Flounder, Paralichthys olivaceus (자성발생성 2배체 넙치의 제 2세대 생산)

  • Jeong Chang Hwa;Moon Young Bong;Park In-Seok;Kim Dong Soo
    • Journal of Aquaculture
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    • v.9 no.3
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    • pp.287-291
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    • 1996
  • [ $F_2$ ] generation of gynogenetic diploids were produced in olive flounder, Paralichthys olivaceus, by brother-sister mating with full-sibling gynogenetic diploids ($F_1$). The induced ovulations and spawnings were conducted by using intraperitoneal injections of HCG (2,000 IU/kg body weight) with photoperiod controls. Floating rates of artificially ovulated eggs were ranged from 22.9 to $65.7\%$. Fertilization and hatching rates were ranged from 69.0 to $86.2\%$ and 36.8 to $85.8\%$, respectively. All of those three rates between two experimental groups were not significantly different (P>0.05). Average survival rates of $F_2$ generation of 40-day-old 효nogenetic diploid larvae were slightly lower than those of controls (P<0.05), however growth rates were much higher than those of their diploid controls.

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Spectral Irradiance and Underwater Transmission Characteristics of a Combined High-Luminance Light-Emitting Diodes as the Light Source for Fishing Lamps (복수 조합에 의한 고휘도 발광 다이오드의 분광분포와 수중투과특성)

  • Choi, Sok-Jin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.42 no.6
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    • pp.703-710
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    • 2009
  • The spectral irradiance and underwater transmission characteristics of a combined high-luminance light-emitting diode (LED) lights have been studied to evaluate suitable light sources for fishing lamps of the next generation. The wavelengths at which the irradiance was maximum were changed from 473, 501, 525, and 465 nm for blue, peacock blue, green, and white LED light to 475, 504 and 528 nm for [$F_{WB}$], [$F_{PB}$] and [$F_{GB}$] combined LED lights, respectively. If the irradiance characteristics at 400-700 nm wavelengths are set as 100%, the irradiance rates at 450-499 nm and 500-549 nm were decreased from 82.4% and 56% for blue, peacock blue LED light to 60.0%, 38.5% for [$F_{WB}$], [$F_{WP}$] combined LED lights. The underwater transmission characteristics of the combined LED lights were superior in the order [$F_{WB}$], [$F_{BP}$], [$F_{GB}$] in optical water type I; [$F_{WB}$], [$F_{PB}$], [$F_{GP}$] in optical water type II-III; and [$F_{GP}$], [$F_{WP}$], [$F_{PB}$] in optical water type 1. Setting the 10m depth underwater transmission characteristics of the combined LED lights in optical water type I at 100%, the transmission of water types II, III and 1 drops to 29.5%, 8.0% and 2.2%. Based on the distribution of spectral irradiance and underwater transmission characteristics calculated in optical water types II-III, where was the jigging ground for fishing lamps, the [$F_{WB}]$ and [$F_{GP}$] combined LED lights can be used as a suitable light sources for fishing lamps of the next generation.

Effect of Ambient Conditions on the Soot Generation of Decane Fuel Droplet (분위기 조건이 Decane 액적의 Soot 생성에 미치는 영향)

  • Lim, Young Chan;Suh, Hyun Kyu
    • Journal of ILASS-Korea
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    • v.19 no.4
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    • pp.211-215
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    • 2014
  • The main purpose of this study is to provide basic information of droplet soot generation of decane fuel. To achieve this, this paper presents the experimental results on the decane droplet combustion conducted under various ambient pressure($P_{amb}$), and oxygen concentration($O_2$) conditions. At the same time, the experimental study was conducted in terms of soot volume fraction($f_v$) and its maximum value. Also, visualization of single fuel droplet was conducted by high resolution CCD camera and ambient pressure($P_{amb}$) and oxygen concentration($O_2$) was changed by control system. It was revealed that higher ambient pressure($P_{amb}$), and oxygen concentration($O_2$) enhanced the soot generation and improved the maximum soot volume fraction( $f_v$).

Studies on the High Protein Mutants of Rice (수도 고단백 돌연변이계통에 관한 연구)

  • Chang-Yawl Harn;J. L. Won
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.20
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    • pp.63-68
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    • 1975
  • Several high protein mutant lines(M4 plant generation, 1974) obtained from X-ray irradiated Jinheung variety were examined at three different locations for their agronomic characters, protein and grain yields. On the other hand, high protein-short culmed-early maturity mutant line No. 398 (M$_{10}$ plant generation, 1974) induced from Hokwang was crossed back to its mother to investigate the gene(s) controlling protein and its pleiotropic relation to other mutated characters. Although variation of protein percent of mutant lines from Jinheung was comparatively large depending on year and location, most of the high protein mutant lines had higher protein yield per unit area than the mother variety and their grain yields were equal to or better than the mother, being resistant to both leaf and neck blast. They were several days earlier-maturing and had shorter-culm except one mutant line. The culm length and heading date of F$_1$ between high protein mutant 398 and its mother Hokwang were intermediate. Accurate assessment of segregation of culm length and heading date in F$_2$ generation and protein percent in F$_3$ seeds will be conducted in 1975.

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A Study on the Evaluation of Barley Hybrids in their Early Generation (보리의 교배조합 검정연구)

  • 김흥배
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.33 no.4
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    • pp.386-391
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    • 1988
  • Five barley crosses and their progenies (F$_2$, F$_3$, and F$_4$) were evaluated the potentiality of hybrid populations to segregate superior yielders in later generation. Four characters used for evaluation were number of spike, number of grain, spike weight and grain weight per plant. Superiority value (Y) of number of spike was best in SB76588${\times}$SB72648 and average of superior plants in F$_4$, was excellent in this cross. Milyang 6${\times}$Suwon 203 showed high Y value and average of F$_4$ superior plants in the number of grain. Spike weight showed the highest Y value and excellent averages of superior plant in Milyang 6${\times}$Suwon 203. Superiority value of grain weight per plant coincided with average of F$_4$ superior plants in 3 crosses and SB76588${\times}$SB72648 was the best among the crosses.

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Neutronic simulation of the CEFR experiments with the nodal diffusion code system RAST-F

  • Tran, Tuan Quoc;Lee, Deokjung
    • Nuclear Engineering and Technology
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    • v.54 no.7
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    • pp.2635-2649
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    • 2022
  • CEFR is a small core-size sodium-cooled fast reactor (SFR) using high enrichment fuel with stainless-steel reflectors, which brings a significant challenge to the deterministic methodologies due to the strong spectral effect. The neutronic simulation of the start-up experiments conducted at the CEFR have been performed with a deterministic code system RAST-F, which is based on the two-step approach that couples a multi-group cross-section generation Monte-Carlo (MC) code and a multi-group nodal diffusion solver. The RAST-F results were compared against the measurement data. Moreover, the characteristic of neutron spectrum in the fuel rings, and adjacent reflectors was evaluated using different models for generation of accurate nuclear libraries. The numerical solution of RAST-F system was verified against the full core MC solution MCS at all control rods fully inserted and withdrawn states. A good agreement between RAST-F and MCS solutions was observed with less than 120 pcm discrepancies and 1.2% root-mean-square error in terms of keff and power distribution, respectively. Meanwhile, the RAST-F result agreed well with the experimental values within two-sigma of experimental uncertainty. The good agreement of these results indicating that RAST-F can be used to neutronic steady-state simulations for small core-size SFR, which was challenged to deterministic code system.

Production of ${\alpha}$1,3-Galactosyltransferase (GalT) Double Knock-out (-/-) Transgenic Pigs for Xenotransplantation (${\alpha}$1,3-Galactosyltransferase(GalT) 유전자가 완전 Knock-out(-/-)된 바이오장기용 형질 전환 돼지 생산)

  • Hwang, Seong-Soo;Oh, Keun-Bong;Kim, Dong-Hoon;Woo, Jea-Seok;Shim, Ho-Sup;Yun, Ik-Jin;Park, Jin-Ki;Im, Gi-Sun
    • Journal of Embryo Transfer
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    • v.27 no.1
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    • pp.9-14
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    • 2012
  • This study was conducted to analyze the transgenic efficiency and sex ratio in ${\alpha}$-1,3-galactosyltransferase (GalT) knock-out (KO) transgenic pigs according to generation. GalT KO piglets were produced by artificial insemination or natural mating. The transgenic confirmation of GalT KO was evaluated by PCR amplification using specific primers. After electrophoresis, three types of bands were detected such as 2.3 kb single band (Wild), 2.3 and 3.6kb double bands (GalT KO -/+; heterozygote), and 3.6kb single band (GalT KO -/-; homozygote). Transgenic efficiency in F1 generation was 64.5% (23/35) of GalT KO (-/+). In F2 generation, GalT KO transgenic efficiency was 36.4% (21/57, Wild), 47.5% (28/57, GalT KO -/+), and 16.1% (8/57, GalT KO -/-), respectively. Interestingly, no homozygote piglets were born in 6 deliveries among total 11 deliveries, although they were pregnant between male (M) and female (F) $F_1$ heterozygote. In the 5 litters including at least one GalT KO -/- piglet, the transgenic efficiency was 13.3% (2/24, Wild), 51.3% (14/24, GalT KO -/+), and 35.3% (8/24, GalT KO -/-), respectively. The sex ratio of M and F was 40:60 in $F_1$ and 49:51 in $F_2$ generation, respectively. Based on these results, GalT KO transgenic pigs have had a reproductive ability with a normal range of transgenic efficiency and sex ratio.