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Effects of intrauterine growth restriction during late pregnancy on the cell growth, proliferation, and differentiation in ovine fetal thymuses

  • Zi, Yang;Ma, Chi;He, Shan;Yang, Huan;Zhang, Min;Gao, Feng;Liu, Yingchun
    • Animal Bioscience
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    • 제35권7호
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    • pp.989-998
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    • 2022
  • Objective: This study investigated the effects of intrauterine growth restriction (IUGR) during late pregnancy on the cell growth, proliferation, and differentiation in ovine fetal thymuses. Methods: Eighteen time-mated Mongolian ewes with singleton fetuses were allocated to three groups at d 90 of pregnancy: restricted group 1 (RG1, 0.18 MJ ME/body weight [BW]0.75/d, n = 6), restricted group 2 (RG2, 0.33 MJ ME/BW0.75/d, n = 6) and control group (CG, ad libitum, 0.67 MJ ME/BW0.75/d, n = 6). Fetuses were recovered at slaughter on d 140. Results: The G0/G1 phase cell number in fetal thymus of the RG1 group was increased but the proliferation index and the expression of proliferating cell nuclear antigen (PCNA) were reduced compared with the CG group (p<0.05). Fetuses in the RG1 group exhibited decreased growth hormone receptor (GHR), insulin-like growth factor 2 receptor (IGF-2R), and their mRNA expressions (p<0.05). For the RG2 fetuses, there were no differences in the proliferation index and PCNA expression (p>0.05), but growth hormone (GH) and the mRNA expression of GHR were lower than those of the CG group (p<0.05). The thymic mRNA expressions of cyclin-dependent protein kinases (CDKs including CDK1, CDK2, and CDK4), CCNE, E2-factors (E2F1, E2F2, and E2F5) were reduced in the RG1 and RG2 groups (p<0.05), and decreased mRNA expressions of E2F4, CCNA, CCNB, and CCND were occurred in the RG1 fetuses (p<0.05). The decreased E-cadherin (E-cad) as a marker for epithelial-mesenchymal transition (EMT) was found in the RG1 and RG2 groups (p<0.05), but the OB-cadherin which is a marker for activated fibroblasts was increased in fetal thymus of the RG1 group (p<0.05). Conclusion: These results indicate that weakened GH/IGF signaling system repressed the cell cycle progression in G0/G1 phase in IUGR fetal thymus, but the switch from reduced E-cad to increased OB-cadherin suggests that transdifferentiation process of EMT associated with fibrogenesis was strengthened. The impaired cell growth, retarded proliferation and modified differentiation were responsible for impaired maturation of IUGR fetal thymus.

옥수수 뿌리로부터 분리한 Membrane-bound ATPase의 특성에 관한 연구 (Characterization of the Membrane-bound Adenosine Triphosphatase from Corn Roots)

  • Moon, Hye Yeon;Kwang Soo Roh;Woong Seop Sim
    • Journal of Plant Biology
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    • 제24권4호
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    • pp.171-179
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    • 1981
  • 옥수수 뿌리로부터 분리한 13,000g pellet과 13,000~80,000g pellet 내에 있는 membrane-bound ATPases의 특성을 구명하였다. 13,000g pellet과 13,000~80,000g pellet의 membrane-bound ATPases의 최적 pH는 5와 9였다. Discontinuous sucrose gradient centrifugation에 의한 13,000g pellet의 분획중 Fraction C는 pH 5에서, Fraction D, E 및 F는 pH 5에서보다 pH 9에서 더높은 활성을 나타냈다. 13,000~80,000g pellet의 분획에서 보면, Fraction A, C는 pH 9보다 pH 5에서, Fraction B, D, E 및 F는 pH 5보다 pH 9에서 더 높은 활성을 가겠다. pH 5와 pH 9에서 membrane-bound ATPases의 기질포화 농도는 3~5 mM이며 ATP에 대한 Km 값은 모두 0.25 mM이였다. Vmax 값은 8.0~55.6 $\mu$M Pi/mg membrane protein/hr의 범위에 있었다. Membrane-bound ATPase의 활성은 $K^+$ 이온에 의해 증가되었다.

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SOME NEW RESULTS ON POWER CORDIAL LABELING

  • C.M. BARASARA;Y.B. THAKKAR
    • Journal of applied mathematics & informatics
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    • 제41권3호
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    • pp.615-631
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    • 2023
  • A power cordial labeling of a graph G = (V (G), E(G)) is a bijection f : V (G) → {1, 2, ..., |V (G)|} such that an edge e = uv is assigned the label 1 if f(u) = (f(v))n or f(v) = (f(u))n, For some n ∈ ℕ ∪ {0} and the label 0 otherwise, then the number of edges labeled with 0 and the number of edges labeled with 1 differ by at most 1. In this paper, we investigate power cordial labeling for helm graph, flower graph, gear graph, fan graph and jewel graph as well as larger graphs obtained from star and bistar using graph operations.

POWER CORDIAL GRAPHS

  • C.M. BARASARA;Y.B. THAKKAR
    • Journal of applied mathematics & informatics
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    • 제42권2호
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    • pp.445-456
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    • 2024
  • A power cordial labeling of a graph G = (V (G), E(G)) is a bijection f : V (G) → {1, 2, ..., |V (G)|} such that an edge e = uv is assigned the label 1 if f(u) = (f(v))n or f(v) = (f(u))n, for some n ∈ ℕ ∪ {0} {0} and the label 0 otherwise, then the number of edges labeled with 0 and the number of edges labeled with 1 differ by at most 1. In this paper, we study power cordial labeling and investigate power cordial labeling for some standard graph families.

인공근육에 적용되는 압전복합재료 작동기의 탄소섬유 배향각과 작동변위의 관계 (Relationship Between CFRP Ply Orientation and Performance Stroke in Piezoelectric Zirconate Titanate Composite Actuator(PZTCA) of Artificial Muscle)

  • 김철웅;이성혁;윤광준
    • 한국정밀공학회:학술대회논문집
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    • 한국정밀공학회 2005년도 추계학술대회 논문집
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    • pp.641-644
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    • 2005
  • The aim of this research is to evaluate the relationship between the total effective moment $(M^E)$ and Bemoulli-Euler bending moment (M) when the ply orientations of UD CFRP in Piezoelectric Zirconate Titanate Composite Actuator (PZTCA) are changed. The obtained results as follows. Firstly, as the performance test results by the CFRP ply orientation, the performance of [0] and [90] were stable. However, while the performance of [+45] was suddenly decreased after 5 hours. Secondly, the change of $M^E$ by the CFRP ply orientation was evaluated. As the CFRP ply orientation was increased from [0] to [+60], the $M^E$ were gradually decreased. However, they became a little bit increased from [+60] to [90]. Finally, after the change of M by the CFRP ply orientation was evaluated, it was found that $M^E=2.2M$ was valid for just [0] and that there was a relationship between $M^E$ and M according to the ply orientation.

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A PROPERTY OF COFUNCTORS SF(X,A)

  • So, Kwang Ho
    • Kyungpook Mathematical Journal
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    • 제13권2호
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    • pp.235-240
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    • 1973
  • A k-dimensional vector bundle is a bundle ${\xi}=(E,P,B,F^k)$ with fibre $F^k$ satisfying the local triviality, where F is the field of real numbers R or complex numbers C ([1], [2] and [3]). Let $Vect_k(X)$ be the set consisting of all isomorphism classes of k-dimensional vector bundles over the topological space X. Then $Vect_F(X)=\{Vect_k(X)\}_{k=0,1,{\cdots}}$ is a semigroup with Whitney sum (${\S}1$). For a pair (X, A) of topological spaces, a difference isomorphism over (X, A) is a vector bundle morphism ([2], [3]) ${\alpha}:{\xi}_0{\rightarrow}{\xi}_1$ such that the restriction ${\alpha}:{\xi}_0{\mid}A{\longrightarrow}{\xi}_1{\mid}A$ is an isomorphism. Let $S_k(X,A)$ be the set of all difference isomorphism classes over (X, A) of k-dimensional vector bundles over X with fibre $F^k$. Then $S_F(X,A)=\{S_k(X,A)\}_{k=0,1,{\cdots}}$, is a semigroup with Whitney Sum (${\S}2$). In this paper, we shall prove a relation between $Vect_F(X)$ and $S_F(X,A)$ under some conditions (Theorem 2, which is the main theorem of this paper). We shall use the following theorem in the paper. THEOREM 1. Let ${\xi}=(E,P,B)$ be a locally trivial bundle with fibre F, where (B, A) is a relative CW-complex. Then all cross sections S of ${\xi}{\mid}A$ prolong to a cross section $S^*$ of ${\xi}$ under either of the following hypothesis: (H1) The space F is (m-1)-connected for each $m{\leq}dim$ B. (H2) There is a relative CW-complex (Y, X) such that $B=Y{\times}I$ and $A=(X{\times}I)$ ${\cap}(Y{\times}O)$, where I=[0, 1]. (For proof see p.21 [2]).

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Clues to Understand the Regulatory Metabolisms Governing the Biosynthesis of Fungal Secondary Metabolites

  • Choi, Yoon-E
    • 한국균학회소식:학술대회논문집
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    • 한국균학회 2015년도 춘계학술대회 및 임시총회
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    • pp.14-14
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    • 2015
  • Fungi are of particular interest due to their capacity to produce an extensive array of secondary metabolites. While many secondary metabolites have no known functions to the producing fungal organisms, these metabolites have tremendous importance to humans with beneficial (e.g., antibiotics) or detrimental (e.g., mycotoxins) properties. In this study, two important filamentous fungi, Fusarium verticillioides and Mycosphaerella graminicola were selected as target species and the genes regulatory functions on the biosynthesis of secondary metabolisms were studied. Functional genomics including forward and reverse genetics, and proteomics were utilized to better understand the complex secondary metabolism regulations in both F. verticillioides and M. graminicola. Identified genes in either F. verticillioides or M. graminicola background were CPP1 (a putative protein phosphatase gene), GAC1 (encoding a GTPase activating protein), MCC1(encoding c-type cyclin), and the velvet gene, MVE1. Our data suggest that there are diverse regulatory genes on fungal secondary metabolites with distinct or overlapping functional roles.

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Synthesis and Electroluminescent Properties of Cabazolyl Vinylene Derivatives

  • Seo, H.J.;Park, H.C.;Lee, S.E.;Park, J.W.
    • 한국정보디스플레이학회:학술대회논문집
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    • 한국정보디스플레이학회 2003년도 International Meeting on Information Display
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    • pp.952-954
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    • 2003
  • We report the photo-(PL) and electroluminescence (EL) properties of new conjugated compounds based on carbazolyl vinylene moiety, 3,3'-(1,4-phenylene di-2,1-ethenediyl) bis[9-ethyl-(E,E)-9H-carbazole](PEEC) and 3,3'-([1,1'-biphenyl]-4,4'-diyldi-2,1-ethenediyl)bis[9-ethyl-9H-carbazole](BPEEC), as emitting materials. The ITO/m-MTDATA/NPB/BPEEC/Alq3/LiF/Al device shows bluish-green EL spectrum at 490nm and turn-on voltage at 8V. PEEC shows bluish-green EL around ${\lambda}$ max=496nm and turn-on voltage at 6V and 2.4 Cd/A efficiency in ITO/m-MTDATA/NPB/PEEC/Alq3/LiF/Al device.

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비정질 TbFeCo 합금 증착박막의 자기광학효과 (Magneto-optic effect of thermal evaporated amorphous TbFeCo thin flim)

  • 박용관;양계준
    • E2M - 전기 전자와 첨단 소재
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    • 제5권4호
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    • pp.439-445
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    • 1992
  • 최근 광자기 기록매체로 주목받고 있는 RE-TM계 비정질 박막을 T $b_{x}$ (F $e_{0.9}$ $Co_{0.1}$)$_{100-x}$ (x=14, 17, 20, 23, 27[at%])의 조성비를 갖는 모합금을 만든후 진공증착방법으로 합금박막을 제작하였다. 각시료의 조성에 따른 자기광학효과를 알아보기 위하여 80K부터 600K에서 포화자화( $M_{s}$ )와 보자력( $H_{c}$)에 대한 온도의존성과 자기이방성상수(Ku), Polar Kerr 이력곡선을 측정하였다. 또한 Curie 온도 근처에서 열처리 시간에 따른 자기 토크 곡선의 변화를 분석 함으로써 합금박막 표면의 산화정도를 정성적으로 평가할 수 있었다. 실험결과 T $b_{22.72}$(F $e_{68.78}$ $Co_{8.5}$)인 시료가 상온에서 8.4KOe의 보자력을 가졌으며 T $b_{x}$(x=23[at%])인 경우가 가장 큰 수직자기이방성을 나타냈다. Polar Kerr 이력곡선은 Tb함량 x=25~26[at%]에서 부호가 반전됨을 알 수 있었다.다.다.다.

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공침법에 의한 $Ni_2Y$ 자성 분말의 합성 (Synthesis of $Ni_2Y$ magnetic particles by coprecipitation method)

  • 김한근;사공건
    • E2M - 전기 전자와 첨단 소재
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    • 제9권9호
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    • pp.906-910
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    • 1996
  • Ferroxplana N $i_{2}$Y(B $a_{2}$N $i_{2}$F $e_{12}$ $O_{22}$ ) magnetic particles, which is one of the hexagonal ferrite were synthesized by a coprecipitation method. The coprecipitates were prepared by adding aqueous solution of BaC $I_{2}$ - 2 $H_{2}$O, NiC $I_{2}$ - 6 $H_{2}$O and FeC $I_{3}$ - 6 $H_{2}$O(of which the mole ratio is $Ba^{+2}$ : N $i^{+2}$ : F $e^{3+}$= 1 : 1 : 6) to a mixture of NaOH and N $a_{2}$C $O_{3}$. The shape of Ferroxplana N $i_{2}$Y magnetic particles obtained at 1, 100(.deg. C) was hexagonal plate-like, average particle size and aspect ratio were 2(.mu.m) and 7, respectively.y.

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