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Fusarium Species from Sorghum in Thailand

  • Mohamed Nor, Nik M.I.;Salleh, Baharuddin;Leslie, John F.
    • The Plant Pathology Journal
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    • v.35 no.4
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    • pp.301-312
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    • 2019
  • Sorghum is the fifth most important cereal worldwide, spreading from Africa throughout the world. It is particularly important in the semi-arid tropics due to its drought tolerance, and when cultivated in Southeast Asia commonly occurs as a second crop during the dry season. We recovered Fusarium from sorghum in Thailand and found F. proliferatum, F. thapsinum and F. verticillioides most frequently, and intermittent isolates of F. sacchari and F. beomiforme. The relatively high frequencies of F. proliferatum and F. verticillioides, suggest mycotoxin contamination, particularly fumonisins and moniliformin, should be evaluated. Genetic variation within the three commonly recovered species was characterized with vegetative compatibility, mating type, Amplified Fragment Length Polymorphisms (AFLPs), and female fertility. Effective population number ($N_e$) was highest for F. verticillioides and lowest for F. thapsinum with values based on mating type allele frequencies higher than those based on female fertility. Based on AFLP genetic variation, the F. thapsinum populations were the most closely related, the F. verticillioides populations were the most distantly related, and the F. proliferatum populations were in an intermediate position. The genetic variation observed could result if F. thapsinum is introduced primarily with seed, while F. proliferatum and F. verticillioides could arrive with seed or be carried over from previous crops, e.g., rice or maize, which sorghum is following. Confirmation of species transmission patterns is needed to understand the agricultural systems in which sorghum is grown in Southeast Asia, which are quite different from the systems found in Africa, Australia, India and the Americas.

Regeneration Studies in Grateloupia filicina (J.V. Lamouroux) C. Agardh - An Important Carrageenophyte and Edible Seaweed

  • Baweja, Pooja;Sahoo, Dinabandhu
    • ALGAE
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    • v.24 no.3
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    • pp.163-168
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    • 2009
  • Grateloupia filicina (J.V. Lamouroux) C. Agardh (Halymeniaceae, Cryptonemiales, Rhodophyta) is an edible seaweed as well as an important source of carrageenan. In the present study, attempt has been made to develop a suitable protocol for effective regeneration of the seaweed and the rapid multiplication of the desired varieties. The young upright thallus of G. filicina was grown in axenic culture using both solid and liquid media. The various media tested were f/2, Provasoli’s Enriched Seawater (PES) and Enriched Seawater (ESW). The effect of glycerol (as a carbon source) and various plant growth regulators i.e. auxin (NAA) and cytokinins (Kinetin and BA) were tested. Although, regeneration of young thalli was observed from the cut ends in all the media, better growth was found in f/2, PES, f/2 (0.5% Glycerol), f/2 (NAA ${10^{-5}}_M)\;and\;f/2\;(BA\;{10^{-6}}_M$). On the other hand callusing was observed only in solid media supplemented with low concentration of Glycerol (0.5%) in f/2, NAA ${10^{-5}}_M\;in\;f/2,\;PES\;and\;BA\;{10^{-5}}_M$ in f/2. Young thalli were developed from the callus sub culture after 40 days of inoculation.

A CHANGE OF SCALE FORMULA FOR CONDITIONAL WIENER INTEGRALS ON CLASSICAL WIENER SPACE

  • Yoo, Il;Chang, Kun-Soo;Cho, Dong-Hyun;Kim, Byoung-Soo;Song, Teuk-Seob
    • Journal of the Korean Mathematical Society
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    • v.44 no.4
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    • pp.1025-1050
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    • 2007
  • Let $X_k(x)=({\int}^T_o{\alpha}_1(s)dx(s),...,{\int}^T_o{\alpha}_k(s)dx(s))\;and\;X_{\tau}(x)=(x(t_1),...,x(t_k))$ on the classical Wiener space, where ${{\alpha}_1,...,{\alpha}_k}$ is an orthonormal subset of $L_2$ [0, T] and ${\tau}:0 is a partition of [0, T]. In this paper, we establish a change of scale formula for conditional Wiener integrals $E[G_{\gamma}|X_k]$ of functions on classical Wiener space having the form $$G_{\gamma}(x)=F(x){\Psi}({\int}^T_ov_1(s)dx(s),...,{\int}^T_o\;v_{\gamma}(s)dx(s))$$, for $F{\in}S\;and\;{\Psi}={\psi}+{\phi}({\psi}{\in}L_p(\mathbb{R}^{\gamma}),\;{\phi}{\in}\hat{M}(\mathbb{R}^{\gamma}))$, which need not be bounded or continuous. Here S is a Banach algebra on classical Wiener space and $\hat{M}(\mathbb{R}^{\gamma})$ is the space of Fourier transforms of measures of bounded variation over $\mathbb{R}^{\gamma}$. As results of the formula, we derive a change of scale formula for the conditional Wiener integrals $E[G_{\gamma}|X_{\tau}]\;and\;E[F|X_{\tau}]$. Finally, we show that the analytic Feynman integral of F can be expressed as a limit of a change of scale transformation of the conditional Wiener integral of F using an inversion formula which changes the conditional Wiener integral of F to an ordinary Wiener integral of F, and then we obtain another type of change of scale formula for Wiener integrals of F.

ON INTEGRAL MEANS OF DERIVATIVES OF UNIVALENT FUNCTIONS

  • Elhosh, M.M.
    • Bulletin of the Korean Mathematical Society
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    • v.24 no.1
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    • pp.13-17
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    • 1987
  • Let S denote the class of nivalent functions normalized so that f(0)=f'(0)-1=0 in vertical bar z vertical bar <1. Let $S_{\alpha}$$^{*}$, -.pi./2<.alpha.<.pi./2, denote the subclass of S that satisfies Re $e^{i{\alpha}}$zf'(z)/f(z).geq.0 in vertical bar z vertical bar <1; then f is called .alpha.-spiral-like and the case .alpha.=0 is the class of normalized starlike functions [6, pp.52]. Let T denote the class of functions f normalized as above and satisfying Im z[Im f(z)]..geq.0 in vertical bar z vertical bar <1; then f is called typically real and T contains those functions of S whose coefficients are real [6, pp.55]. Also, in view of [6, pp.231], let B(.lambda.) be the class of function normalized as above and map vertical bar z vertical bar <1 onto the complement of an arc with radial angle .lambda.(0<.lambda.<.pi./2). The radial angle is meant to be the angle between the tangent and radial vectors to the arc. This note includes a sharp version for Corollary 1 of [2] when f.mem. $S_{\alpha}$$^{*}$ as well as a logarithmic coefficient estimate.nt estimate.

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Relationship between production of exoenzymes and serotypes of Pseudomonas aeruginosa isolated from clinical specimens and hospital environments (녹농균(綠膿菌)의 균체외효소산생능(菌體外酵素産生能)과 그의 혈청형(血淸型)과의 관계(關係))

  • Moon, Hong-Yong;Cho, Yag-Ja
    • The Journal of the Korean Society for Microbiology
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    • v.15 no.1
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    • pp.47-54
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    • 1980
  • Exoenzymes, protease(P) and elastase(E) produced by Pseudomonas aeruginosa are reported to have close relationship with pathogenicity of Pseudomonas aeruginosa. Productibility of exoenzymes P and E were studied and compared in environmental isolates from hospital environments and clinical isolates from various clinical specimens, also, the relationship between their enzyme production and serotype were reviewed. 1. Clinical isolates were typed into nine serotypes A, B, C, D, E, F, G, H and I. Serotype E had the highest incidence of 24%, followed by B with 16.8%, G, 15.1% and C, 9.3%. 2. Environmental isolates were, typed as serotype B, C, E, F, G, H, I, K and M. Serotype I had the highest incidence of 26.6%, followed by C, F and M each incidence of 14.3%. 3. In the typing of the above two groups, serotypes A and D were found only in the clinical isolates and serotypes K, and M were found only in the environmental isolates. Serotypes J and L were found in neither clinical isolates nor environmental isolates. 4. In the distribution of serotypes from various clinical specimens, serotype G among isolates from pus showed incidence of 20.4%, and serotypes E and B were 19.5% separately. Serotype E had incidence of 22.6% and 20.0% in urine and sputa respectively, showing a high rate compared to the other serotypes. 5. The incidence of strains producing both exoenzymes P and E was 77.8% in the preserved strains of clinical isolates and 76.2% in the environmental isolates. There were no significant difference between the two groups. 6. Serotypes A and H, which are preserved strains from clinical isolates showed productibility of both exoenzymes P and E, the other serotypes showed productibility of various combination of exoenzymes. Among the environmental isoaltes, production of both exoenzymes P and E were seen in serotypes E, F, G, H, I and K and no serotype produced only P or E. 7. In ability to produce exoenzymes of isolates from sources of various clinical specimens, strains producing both exoenzymes P and E were found most frequently in pus with incidence rate of 82.0%, followed by 80.0% in sputum and urine. 8. Almost all the fresh strains of clinical isolates were producers of both exoenzymes P and E.

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Synthesis of Nucleophilic Adducts of Thiols (Ⅳ). Addition of Glutathione to $\beta$-Nitrostyrene Derivatives

  • Kim, Tae-Rin;Choi, Sung-Yong;Choi, Won-Sik
    • Bulletin of the Korean Chemical Society
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    • v.4 no.2
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    • pp.92-95
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    • 1983
  • The addition products of glutathione to ${\beta}$ -nitrostyrene derivatives were synthesized. ${\beta}$ -Nitrostyrene (1a), p-methyl-${\beta}$-nitrostyrene (1b), 3,4,5-trimethoxy-${\beta}$-nitrostyrene (1c), o-, m- and p-chloro-${\beta}$-nitrostyrene (1e, 1f, 1g) and o-, m- and p-methoxy-${\beta}$-nitrostyrene (1h, 1i, 1j) undergo addition reactions with glutathione to form S-(2-nitro-1-phenylethyl)-L-glutathione (5a), S-[2-nitro-1-(p-methyl)phenylethyl]-L-glutatione (5b), S-[2-nitro-1-(3', 4', 5'-trimethoxy)phenylethyl]-L-glutathione (5c), S-[2-nitro-1-(o-chloro)phenylethyl]-L-glutathione (5e), S-[2-nitro-1-(m-choro)phenylethyl]-L-glutathione (5f), S-[2-nitro-1-(p-chloro)phenylethyl]-L-glutathione (5g), S-[2-nitro-x-(o-methoxy)-phenylethyl]-L-glutathion e(5h), S-[2-nitro-x-(m-methoxy)phenylethyl]-L-glutathion e (5i), and S-[2-nitro-1-(p-methoxy)phenylethy])-L-glutathione (5j), respectively. The structure of adducts were identified by UV and IR-spectra, molecular weight measurement, and elemental analysis.

Transcriptional Activation and Repression of Cell Cycle Regulatory Molecules by Trichostatin A (Trichostatin A 처리에 의하 세포주기 조절인자들의 전사활성화 및 불활성화)

  • Baek Jong-Soo;Lee Hee-Kyung;Cho Young-Su;Kim Sung-Young;Park Kwan-Kyu;Chang Young-Chae
    • Journal of Life Science
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    • v.15 no.6 s.73
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    • pp.994-1004
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    • 2005
  • The dihydrofolate reductase (dhfr) promoter contains cis-acting element for the transcription factors Spl and E2F. Transcription of dhfr gene shows maximal activity during the Gl/S phase of cell cycle. The member of the Spl transcriptional factor family can act as both negative and positive regulators of gene expression. There was a report that Spl-Rb and E2F4-pl30 complexes cooperate to establish stable repression of dhfr gene expression in CHOC400 cells. Here, we examined the role of HDAC in dhfr, cyclin E, and cyclin A gene regulation using the histone deacetylation inhibitor, trichostatin A (TSA) in U2OS and C33A cells, a Rb-positive human osteosarcoma cell line, and a Rb-negative cervical carcinoma cell line, respectively. When the dhfr promoter constructs were applied in U2OS cells, TSA markedly stimulated over 14-fold of dhfr promoter activity through dhfr-Spl sites by the deletion of an E2F element. In contrast, the deletion of dhfr-Spl binding sites completely abolished promoter stimulation by TSA. The dhfr promoter activity including dhfr-Spl sites increased only 2-fold in C33A cells. Promoter activity containing only dhfr-E2F site did not have much effect by the treatment of TSA in both U2OS and C33A cells. On the other hand, treatment with TSA induced significantly mRNA expression of dhfr and cyclin E, whereas levels of cyclin A decreased in U2OS cells, but had no effect in C33A cells. These results indicate that TSA have contradictory effect, activation of dhfr and cyclin E genes on Gl phase, and down-regulation of cyclin A on G2 phase through transcriptional regulation in U2OS cells.

A comparative analysis of volatile organic compound levels in field samples between different gas chromatographic approaches (분석기법의 차이에 따른 현장시료의 VOC 분석결과 비교연구: 분석오차의 발생 양상과 원인)

  • Ahn, Ji-Won;Pandey, Sudhir Kumar;Kim, Ki-Hyun
    • Analytical Science and Technology
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    • v.23 no.5
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    • pp.465-476
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    • 2010
  • In this study, a number of volatile organic compounds (VOCs) including benzene, toluene, p-xylene, styrene, and methyl ethyl ketone were analyzed from samples collected in ambient air and under the field conditions. These samples were analyzed independently by two different set-ups for VOC analyses, i.e., between [1] gas chromatography/flame ionization detector with tube sampling - (F-T system) and [2] gas chromatography/mass spectrometer with bag sampling (M-B system). The analytical results derived by both systems showed fairly similar patterns in relative sense but with moderately large differences in absolute sense. The results of M-B system were high relative to F-T system with the F-T/M-B ratio below 1. If the relative biases of the two measurement techniques are derived in terms of percent difference (PD) in concentration values, the results were generally above 35% on average. A student t-test was applied to investigate the statistical significance of those differences between the systems. The results of both analytical systems were different at 95% confidence level for toluene, p-xylene, styrene, and methyl ethyl ketone (P < 0.043). However, F-T and M-B systems showed strong correlations for toluene and p-xylene. The observed bias is explained in large part by such factors as the differences in standard phases used for each system and the chemical loss inside the bag sampler.

Evidence of Spin Reorientation by Mössbauer Analysis

  • Myoung, Bo Ra;Kim, Sam Jin;Kim, Chul Sung
    • Journal of Magnetics
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    • v.19 no.2
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    • pp.126-129
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    • 2014
  • We report the crystallographic and magnetic properties of $Ni_{0.3}Fe_{0.7}Ga_2S_4$ by means of X-ray diffractometer (XRD), a superconducting quantum interference device (SQUID) magnetometer, and a M$\ddot{o}$ssbauer spectroscopy. In particular, $Ni_{0.3}Fe_{0.7}Ga_2S_4$ was studied by M$\ddot{o}$ssbauer analysis for evidence of spin reorientation. The chalcogenide material $Ni_{0.3}Fe_{0.7}Ga_2S_4$ was fabricated by a direct reaction method. XRD analysis confirmed that $Ni_{0.3}Fe_{0.7}Ga_2S_4$ has a 2-dimension (2-D) triangular lattice structure, with space group P-3m1. The M$\ddot{o}$ssbauer spectra of $Ni_{0.3}Fe_{0.7}Ga_2S_4$ at spectra at various temperatures from 4.2 to 300 K showed that the spectrum at 4.2 K has a severely distorted 8-line shape, as spin liquid. Electric quadrupole splitting, $E_Q$ has anomalous two-points of temperature dependence of $E_Q$ curve as freezing temperature, $T_f=11K$, and N$\acute{e}$el temperature, $T_N=26K$. This suggests that there appears to be a slowly-fluctuating "spin gel" state between $T_f$ and $T_N$, caused by non-paramagnetic spin state below $T_N$. This comes from charge re-distribution due to spin-orientation above $T_f$, and $T_N$, due to the changing $E_Q$ at various temperatures. Isomer shift value ($0.7mm/s{\leq}{\delta}{\leq}0.9mm/s$) shows that the charge states are ferrous ($Fe^{2+}$), for all temperature range. The Debye temperature for the octahedral site was found to be ${\Theta}_D=260K$.