• BMB Reports
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• v.37 no.2
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• pp.199-205
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• 2004

The six lumbrokinase fractions (F1 to F6) with fibrinolytic activities were purified from earthworm Lumbricus rubellus lysates using the procedures of autolysis, ammonium sulfate fractionation, and column chromatography. The proteolytic activities on the casein substrate of the six iso-enzymes ranged from 11.3 to 167.5 unit/mg with the rank activity orders of F2 > F1 > F5 > F6 > F3 > F4. The fibrinolytic activities of the six fractions on the fibrin plates ranged from 20.8 to 207.2 unit/mg with rank orders of F6 > F2 > F5 > F3 > F1 > F4. The molecular weights of each iso-enzyme, as estimated by SDS-PAGE, were 24.6 (F1), 26.8 (F2), 28.2 (F3), 25.4 (F4), 33.1 (F5), and 33.0 kDa (F6), respectively. The plasminogen was activated into plasmin by the enzymes. The optimal temperature of the six iso-enzymes was $50^{\circ}C$, and the optimal pH ranged from pH 4-12. The four iso-enzymes (F1-F4) were completely inhibited by PMSF. The two enzymes (F5 and F6) were completely inhibited by aprotinin, TLCK, TPCK, SBTI, LBTI, and leupeptin. The N-terminal amino acid (aa) sequences of the first 20 to 22 residues of each fraction had high homology. All six isoenzymes had identical aa residues 2-3 and 13-15. The N-terminal 21-22 aa sequences of the F2, F3, and F4 isoenzymes were almost the same. The N-terminal aa sequences of F5 and F6 were identical.

• Clinical and Experimental Reproductive Medicine
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• v.20 no.2
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• pp.157-160
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• 1993

The human follicular f1uids(F.F.) may be considered to contribute the maturation of the oocytes on the in vitro culture. To investigate the effects of epidermal growth factor (E.G.F.), which is present in mature and immature follicular fluids, we had experiments of mouse oocytes maturation in vitro. The endpoints assayed were rated as percentage of oocytes undergoing germinal vesicle breakdown(G.V.B.D.) and polar body(P.B.) formation at 12 hours after in vitro culture. The rates of G.B.B.D. were 87% in mature F.F. 68% in immature F.F. and 78% in Ham's F-10 medium respectively. And overall the mature F.F. seem to stimulate on in vitro oocyte maturation compared with either immature F.F. or Ham's F-10 medium. As the concentration of addition of E.G.F. in immature F.F., the rates of G.V.B.D. and P.B. formation were 82 %, 23% in addition with 2 ng/ml while 84%, 32% in addition with 4 ng/ml respectivly. And at the concentration of addition of E.G.F. in Ham's F-10 media as well, the rates of G.V.B.D. and P.B. formation were 84%, 40% and 82%, 44% in addition with each 2ng, 4ng. AccordinglY there was no influence on the oocytes maturation at the addition of E.G.F. to each immature F.F. and Ham's F-10 medium. In conclusion, the E.G.F. is not able to induce oocyte maturation independent of it's effects in immature F.F. and Ham's F-10 media.

• The Pure and Applied Mathematics
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• v.24 no.2
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• pp.109-127
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• 2017

In this paper, we solve the following quadratic ${\rho}-functional$ inequalities ${\parallel}f({\frac{x+y+z}{2}})+f({\frac{x-y-z}{2}})+f({\frac{y-x-z}{2}})+f({\frac{z-x-y}{2}})-f(x)-f(y)f(z){\parallel}$ (0.1) ${\leq}{\parallel}{\rho}(f(x+y+z)+f(x-y-z)+f(y-x-z)+f(z-x-y)-4f(x)-4f(y)-4f(z)){\parallel}$, where ${\rho}$ is a fixed non-Archimedean number with ${\mid}{\rho}{\mid}$ < ${\frac{1}{{\mid}4{\mid}}}$, and ${\parallel}f(x+y+z)+f(x-y-z)+f(y-x-z)+f(z-x-y)-4f(x)-4f(y)-4f(z){\parallel}$ (0.2) ${\leq}{\parallel}{\rho}(f({\frac{x+y+z}{2}})+f({\frac{x-y-z}{2}})+f({\frac{y-x-z}{2}})+f({\frac{z-x-y}{2}})-f(x)-f(y)f(z)){\parallel}$, where ${\rho}$ is a fixed non-Archimedean number with ${\mid}{\rho}{\mid}$ < ${\mid}8{\mid}$. Using the direct method, we prove the Hyers-Ulam stability of the quadratic ${\rho}-functional$ inequalities (0.1) and (0.2) in non-Archimedean Banach spaces and prove the Hyers-Ulam stability of quadratic ${\rho}-functional$ equations associated with the quadratic ${\rho}-functional$ inequalities (0.1) and (0.2) in non-Archimedean Banach spaces.

• Research in Plant Disease
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• v.29 no.2
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• pp.118-129
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• 2023

Fusarium sambucinum species complex (FSAMSC) is an important taxonomic group, causing severe plant diseases. Many studies were carried out on FSAMSC plant diseases in Korea, but only 2 species (F. graminearum, F. sambucinum) from 14 host plants were registered in the List of Plant Disease in Korea. To clarify FSAMSC diversity and their pathogenecity, we examined FSAMSC isolates preserved in the Korean Agricultural Culture Collection. Fifty-seven strains were reidentifed as 7 species (F. asiaticum, F. graminearum, F. vorosii, F. meridionale, F. boothii, F. kyushuense, F. armeniacum) based on multi-locus sequence typing analysis. According to previous reports and result of this study, 5 species (F. asiaticum, F. graminearum, F. vorosii, F. armeniacum, F. sambucinum) were pathogenic on 24 host plants in FSAMSC, while the pathogenicity of 3 species (F. meridionale, F. boothii, F. kyushuense) were not clear.

• Honam Mathematical Journal
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• v.1 no.1
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• pp.21-25
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• 1979

Abelian군(群)의 presheaf에 관한 직적(直積)과 직화(直和)를 Category 입장에서 정의(定義)하고 presheaf $F_{\lambda}\;({\lambda}{\epsilon}{\Lambda})$들의 두 직적(直積)(또는 直和)은 서로 동형적(同型的) 관계(關係)에 있으며, 특히 ${\phi}:X{\rightarrow}Y$가 homeomorphism이라 하고 ${\phi}_*F$를 X상(上)의 presheaf F의 direct image이라 하면 (1) $({\phi}_*F, \;{\phi}_*(f_{\lambda})_{{\lambda}{\epsilon}{\Lambda}})$가 $({\phi}_*F_{\lambda})_{{\lambda}{\epsilon}{\Lambda}}$의 직적(直積)일 때 오직 그때 한하여 $(F,\;(f_{\lambda})_{{\lambda}{\epsilon}{\Lambda}})$는 $(F_{\lambda})_{{\lambda}{\epsilon}{\Lambda}})$의 직적(直積)이다. (2) $({\phi}_*F,\;{\phi}_*(l_{\lambda})_{{\lambda}{\epsilon}{\Lambda}})$가 $({\phi}_*F_{\lambda})_{{\lambda}{\epsilon}{\Lambda}}$의 직화(直和)일 때 오직 그때 한하여 $(F,\;(l_{\lambda})_{{\lambda}{\epsilon}{\Lambda}})$는 $(F_{\lambda})_{{\lambda}{\epsilon}{\Lambda}})$의 직화(直和)이다. Let $(F_{\lambda})_{{\lambda}{\epsilon}{\Lambda}})$ be an indexed set of presheaves of abelian group on topological space X. We can define the cartesian product $$\prod_{{\lambda}{\epsilon}{\Lambda}}\;F_{\lambda}$$ of $(F_{\lambda})_{{\lambda}{\epsilon}{\Lambda}})$ by $$(\prod_{{\lambda}{\epsilon}{\Lambda}}\;F_{\lambda})(U)=\prod_{{\lambda}{\epsilon}{\Lambda}}(F_{\lambda}(U))$$ for U open in X $${\rho}_v^u:\;(\prod_{{\lambda}{\epsilon}{\Lambda}}\;F_{\lambda})(U){\rightarrow}(\prod_{{\lambda}{\epsilon}{\Lambda}}\;F_{\lambda})(V)((s_{\lambda})_{{\lambda}{\epsilon}{\Lambda}}{\rightarrow}(_{\lambda}{\rho}_v^u(s_{\lambda}))_{{\lambda}{\epsilon}{\Lambda}})$$ for $V{\subseteq}U$ open in X where $_{\lambda}{\rho}^U_V$ is a restriction of $F_{\lambda}$, And we have natural presheaf morphisms ${\pi}_{\lambda}$ and ${\iota}_{\lambda}$ such that ${\pi}_{\lambda}(U):\;({\prod}_\;F_{\lambda})(U){\rightarrow}F_{\lambda}(U)((s_{\lambda})_{{\lambda}{\epsilon}{\Lambda}}{\rightarrow}s_{\lambda})$ ${\iota}_{\lambda}(U):\;F_{\lambda}(U){\rightarrow}({\prod}\;F_{\lambda})(U)(s_{\lambda}{\rightarrow}(o,o,{\cdots}\;{\cdots}o,s_{\lambda},o,{\cdots}\;{\cdots}o)$ for $(s_{\lambda}){\epsilon}{\prod}_{\lambda}\;F_{\lambda}(U)$ and $(s_{\lambda}){\epsilon}F_{\lambda}(U)$.

• Mycobiology
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• v.30 no.3
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• pp.139-145
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• 2002

Gibberella fujikuroi is species complex. This species complex includes Fusarium tabacinum, F. moniliforme(=F. verticillioides), F. nygamai, F. proliferatum as well as F. subglutinans. Our objective was to develop a technique to differentiate between isolates of F. subglutinans, F. proliferatum and F. verticillioides. Thirty-two strains of F. subglutinans, six strains from F. verticillioides and five strains of F. Proliferatum isolated from maize in Austria were studied using random amplified polymorphic DNA(RAPD). F. subglutinans strains clustered very closely, with similarity ranging from $87{\sim}100%$. On the other hand, all the amplification patterns of F. verticillioides were identical, as well as in the case of F. proliferatum. Our results indicated that these Fusaria species are distinct species and hence RAPD markers can be quick and reliable for differentiating them.

• Journal of applied mathematics & informatics
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• v.19 no.1_2
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• pp.127-133
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• 2005

An f-coloring of a graph G = (V, E) is a coloring of edge set E such that each color appears at each vertex v $\in$ V at most f(v) times. The minimum number of colors needed to f-color G is called the f-chromatic index $\chi'_f(G)$ of G. Any graph G has f-chromatic index equal to ${\Delta}_f(G)\;or\;{\Delta}_f(G)+1,\;where\;{\Delta}_f(G)\;=\;max\{{\lceil}\frac{d(v)}{f(v)}{\rceil}\}$. If $\chi'_f(G)$= ${\Delta}$f(G), then G is of $C_f$ 1 ; otherwise G is of $C_f$ 2. In this paper, the classification problem of complete graphs on f-coloring is solved completely.

• The Pure and Applied Mathematics
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• v.23 no.3
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• pp.247-263
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• 2016

Let $M_{1}f(x,y):=\frac{3}{4}f(x+y)-\frac{1}{4}f(-x-y)+\frac{1}{4}f(x-y)+\frac{1}{4}f(y-x)-f(x)-f(y)$, $M_{2}f(x,y):=2f(\frac{x+y}{2})+f(\frac{x-y}{2})+f(\frac{y-x}{2})-f(x)-f(y)$. Using the direct method, we prove the Hyers-Ulam stability of the additive-quadratic ρ-functional inequalities (0.1) $N(M_{1}f(x,y),t){\geq}N({\rho}M_{2}f(x,y),t)$ where ρ is a fixed real number with |ρ| < 1, and (0.2) $N(M_{2}f(x,y),t){\geq}N({\rho}M_{1}f(x,y),t)$ where ρ is a fixed real number with |ρ| < $\frac{1}{2}$.

• Applied Biological Chemistry
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• v.49 no.1
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• pp.25-29
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• 2006

This study was carried out to isolate the possible anti-tumor promoters from the citrus peel (Citrus natsudaidai Hayata). We fractionated the cold-pressed oil of citrus peel by column chromatography, HPLC and TLC. The analysis on column chromate-graphy yielded seven peaks $(F-I{\sim}F-VII)$, all of which showed single spot on TLC analysis ($R_f$ for $F-I{\sim}VIII$; 0.31, 0.13, 0.13, 0.78, 0.79, 0.69 and 0.84). Among the seven fractions, three fractions (F-I, -II and F-IV) were re-analyzed on HPLC, also showing single peak except for one fraction (F-IV) which was divided two peaks. The retention times $(R_f)$ of F-I and F-II was 3 min. and 2.5 min., respectively, but these of two peaks from F-IV were 2 min. and 4.5 min., respectively. Since the area of the latter peak (4.5 min.) was very smaller than that of the former one (2 min.), it is considered that the latter one did not appear on TLC analysis. The inhibitory effect on tumor promoter 12-O-tetradecanoylphorbol-13-acetate(TPA)-induced Epstein-Barr virus activation in Raji cells was tested for the seven fraction obtained. It decreased in order of F-VI (82.3+1.3%) > F-I (80.4+1.6%) > F-II (77.2+0.9%) > F-III (75.0+1.2%) > F-IV (74.1=1.0%) > F-V (71.0+1.1%) > F-VII (70.2+1.2%). These results imply that some constiuents contained in citrus peels have the inhibitory activity of TPA-induced tumor promotion.

• Honam Mathematical Journal
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• v.37 no.4
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• pp.473-486
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• 2015

In this paper, we investigate a stability problem for a functional equation f(-x - y - z) - f(x + y) - f(y + z) - f(x + z) + 2f(x) + 2f(y) + 2f(z) - f(-x) - f(-y) - f(-z) = 0 by applying the direct method.