• Analytical Science and Technology
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• v.19 no.6
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• pp.455-459
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• 2006

The extraction and separation of glabridin from licorice root by HPLC was performed in this work. First, by investigating the different extraction solvents, extraction methods and extraction times, a one-hour ultrasonic extraction procedure with ethyl acetate as the extraction solvent was optimized. Then the ethyl acetate extraction was applied to RP-HPLC for separation of glabridin. The column efficiencies and resolutions were experimentally investigated with different mobile phase compositions. Baseline separation of glabridin was obtained under the mobile phase composition of 50/50 vol.% (ACN/water). The retention time of glabridin was 20.3 min. The peak of glabridin was collected from the HPLC elution for several times and identified by LC/MS. Under the optimum extraction and HPLC separation methods, 1.26 g of glabridin per kg licorice root could be extracted.

• Korean Chemical Engineering Research
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• v.54 no.3
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• pp.315-319
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• 2016

This study examined the optimization of hot-water extraction conditions for maximizing the total polyphenol compounds (TPC) extracted from amaranth leaf. The effects of three independent variables, including extraction temperature, extraction time and ethanol concentration on TPC were investigated using central composite design (CCD). The concentration of TPC increased with increased levels of extraction temperature and time. The extraction temperature and the ethanol concentration showed the significant effect on TPC production (p<0.05). The predicted values at the optimized condition were acceptable when compared to the experimental values ($R^2=0.9566$). The optimum extraction conditions were as follows: temperature of $90.1^{\circ}C$, time of 50 min and ethanol concentration of 61.6% (v/v) for the maximum TPC of 12.6 mg GAE/g DM.

• Culinary science and hospitality research
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• v.14 no.3
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• pp.210-222
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• 2008

This study showed that the brown stock, which is the base of demi-glace sauce, extracted by using a high pressure heating extractor is more advantageous than that extracted by the traditional extraction method for the mass production. We compared the former with the latter in terms of minerals and free amino acids. The results of this study are summarized as follows. When mineral contents were compared, the brown stock extracted by high.pressure heating extraction showed the tendency of increase in mineral contents in proportion to heating temperature and heating time, but, from extraction temperature of 140$^{\circ}C$, the contents of K, Mg, Na and P decreased with the increase of extraction time. In addition, mineral contents in the brown stock extracted by high-pressure heating extraction were generally lower than those in brown stock extracted by the traditional extraction method. This result was produced probably because materials were added repeatedly in the traditional method. Amino acids contents in brown stock according to the extraction methods were also examined. They increased with the increase in the number of extractions in the brown stock extracted by the traditional method, and those in the brown stock extracted using a high pressure heating extractor increased with the increase in heating temperature and extraction time. The results of this study are expected to be useful as a practical material for the mass production of brown stock products.

• Journal of the Korean Society for Precision Engineering
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• v.15 no.6
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• pp.167-174
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• 1998

The ultrasonic test is recognized for its significance as a non-destructive testing method to detect volume defects such as porosity and incomplete penetration which reduce strength in the weld zone. This paper illustrates the defect detection in the weld zone of ferritic carbon steel using ultrasonic wave and the evaluation of pattern recognition by chaotic feature extraction using time series signal of detected defects as data. Shown in the time series data were that the time delay was 4 and the embedding dimension was 6 which indicate the geometric dimension of the subject system and the extent of information correlation. Based on fractal dimension and lyapunov exponent in quantitative chaotic feature extraction, feature value of 2.15, 0.47 is presented for porosity and 2.24, 0.51 for incomplete penetration The precision rate of the pattern recognition is enhanced with these values on the total waveform of defect signal in the weld zone. Therefore, we think that the ultrasonic pattern recognition method of weld zone defects of ferritic carbon steel by ultrasonic-chaotic feature extraction proposed in this paper can boost precision rate further than the existing method applying only partial waveform.

• Korean Journal of Food Science and Technology
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• v.7 no.1
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• pp.30-36
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• 1975

The extraction efficiency of orange-yellow pigment from the Gardenia was greatly depended upon the extraction time, extraction temperature, volume of solvent used and fat contents of the Gardenia. From the experimental results, the amounts of extracted pigment (P) was proportional to the $log\;t^{\;1{\cdot}15}$ of extraction time$(t;\;0{\sim}60\;min.)$, the $log\;T^{3{\cdot}73}$ of extraction temperature$(T;\;5{\sim}60^{\circ}C)$, the $log\;S^{3{\cdot}7}$ of volume of solvent$(S;\;5{\sim}50\;ml)$, and the -4X of fat contexts of sample $(X;\;0{\sim}0.\;15)$ at $18^{\circ}C$ for 10 minutes. Finally, the modified empirical equation was derived as follow; $P{\simeq}1.15\;log\;t+3.73\;log\;T+3.7\;log\;S-4X-6.4$ In addition to that, the most optimum conditions of pigment extraction were determined as 30 minutes of operation time, $40^{\circ}C$ of temperature. Deffated Gardenia was more productive than natural Gardenia in the pigment extraction.

• Applied Chemistry for Engineering
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• v.30 no.4
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• pp.408-414
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• 2019

In this study, an antioxidant was extracted from dandelion leaves using traditional hot water and ultrasonic extraction methods. In order to optimize the extraction yield and total flavonoid, an antioxidant, Box-Behnken design (BBD) model among response surface analysis methods was used. In the case of hot water extraction, the extraction temperature and time as well as the ratio of alcohol/ultrapure water were set as variables, and for the ultrasonic extraction, the ultrasonic survey century and irradiation time and the ratio of alcohol/ultrapure water were variables. Optimum extraction conditions in the hot water extraction method were the extraction temperature and time of $45.76^{\circ}C$ and 1.75 h and the ratio of alcohol/ultrapure water of 41.92 vol.%. While for the ultrasonic extraction method the survey century of 512.63 W, the ratio of alcohol/ultrapure water of 56.97 vol.% and the extraction time of 20.79 min were optimum conditions. Expected reaction yield and flavonoid content values under the optimized condition were calculated as 22.09 wt.% and 28.98 mg QE/mL dw, respectively. In addition, the error value of less than 3% was obtained validating our optimization process.

• Food Science and Biotechnology
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• v.17 no.5
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• pp.899-903
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• 2008

This study was conducted in order to elucidate the optimum conditions for the extraction of clove that can be used to elicit antibacterial activity against Streptococcus mutans using the evolutionary operation (EVOP)-factorial design technique. Higher antibacterial activity was achieved in a higher extraction temperature of $80^{\circ}C$ ($r=0.7983^{**}$) and in a longer extraction time of 26 hr ($r=0.6867^*$). Antibacterial activity was not effected by differentiation of ethanol concentration in the extraction solvent (r=-0.0683). The maximum antibacterial activity of clove against S. mutans as determined by the EVOP-factorial design technique was obtained at an extraction temperature of $80^{\circ}C$, an extraction time of 26 hr and a 50% ethanol concentration. Furthermore, the population of S. mutans decreased from an initial concentration of 6.850 to 4.195 log CFU/mL in the third set that is more than 2.6 log cycles by EVOP-factorial design technique.

• Food Science and Biotechnology
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• v.14 no.2
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• pp.190-195
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• 2005

The conditions for the extraction of effective substances from the stem of Opuntia ficus-indica were optimized by a response surface methodology. The total extract yield was maximized under the temperature of $97.77^{\circ}C$, at a time duration of 145.82 min and a water to sample ratio 16.59 mL/g. Moreover, the optimum conditions for the extraction of effective substances were as follows: $84.95^{\circ}C$, 156.50 min and a water/sample ratio of 7.46 mL/g for the phenolics content; and $97.11^{\circ}C$, 139.03 min and a water/sample ratio of 10.91 mL/g for the pectin content. The range of optimum extraction conditions in consideration of the physicochemical properties of the extracts were shown to be as $95-100^{\circ}C$ as the extraction temperature, 120-180 min as extraction time and a water to sample ratio of 5-18 mL/g.

• Korean Chemical Engineering Research
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• v.55 no.4
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• pp.503-509
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• 2017

The present experimental study proposed two ionic liquids (ILs) namely [Aliquat 336] [$HSO_4$] (prepared and characterized at our laboratory) and Cyphos 101 IL (supplied by Cytec Company) dissolved in two different diluents such as DCM (di-chloro-methane) and toluene applied for PMs extraction. The first IL [Aliquat 336] [$HSO_4$] prepared and confirmed the formation of final product by using FT-IR and TGA studies. The primary experiment in solvent extraction processing is kinetic effect; 0 to 30 time varied for PMs by using two ILs and confirmed the optimized extraction equilibrium time. This study was conducted for PMs (Pt, Rh and Cu) extraction and separation from each other by using proposed ILs. This is the primary study of the utilizing green solvents such as ILs as an extractant system for Pt, Rh and Cu extraction and possible separation.

• KSBB Journal
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• v.24 no.2
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• pp.212-215
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• 2009

In this study, the process parameters of liquid-liquid extraction were optimized to obtain a high purity and yield of paclitaxel in a pre-purification step. The optimal solvent ratio (methylene chloride/concentrated methanol extract ratio), extraction times, mixing time, and standing time for liquid-liquid extraction were 0.28 (v/v), 3(times), 30 min, and 40 min, respectively. The polar impurities from the biomass extraction were efficiently removed by liquid-liquid extraction. The complete concentration of liquid-liquid extract by rotary evaporator was reliable enough to obtain a high purity and yield of paclitaxel for subsequent purification steps.